Expression of Metallothioneins in Placental and Fetal Tissues in Undisturbed and PGM-Zn Treated Syngeneic Pregnancy

Hrvoje Jakovac; Damir Grebić; Ines Mrakovcic-Šutić; Daniel Rukavina; Biserka Radošević-Stašić

doi:doi:10.11648/j.ajbio.s.2015030202.12

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Expression of Metallothioneins in Placental and Fetal Tissues in Undisturbed and PGM-Zn Treated Syngeneic Pregnancy

Hrvoje Jakovac, Damir Grebić, Ines Mrakovcic-Šutić, Daniel Rukavina, Biserka Radošević-Stašić

Published in American Journal of BioScience (Volume 3, Issue 2-2)

Received: 16 January 2015 Accepted: 19 January 2015 Published: 2 March 2015

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Abstract

Metallothioneins (MTs) are cysteine-rich proteins, which have been implicated in regulation of physiological processes, such as cell growth, repair, differentiation, apoptosis and immunoregulation, as well as in the protection against heavy metals, oxidant damages, inflammation and other stressful conditions. To investigate their roles in physiological and detoxification processes at the maternal-fetal interface and in fetal organogenesis we examined the tissue expression of MT I/II isoforms in undisturbed syngeneic pregnancy and after the treatment with peptidoglycan monomer linked with zinc (PGM-Zn). The data showed that in undisturbed pregnancy the MTs were highly expressed at junctional zone of placenta, on endovascular trophoblast giant cells, glycogen cells and spongiotrophoblast cells, as well as on villous trophoblast cells. In the fetus they were found predominantly in the liver and in epithelial tissues, such as intestine, pancreas and kidney. Treatment with PGM-Zn markedly enhanced the intensity of MT staining both at the maternal-fetal interface and in fetal organs. The data imply that MTs are involved in the protection of trophoblast cells against the pregnancy-induced deregulation of redox and neuro-immuno-endocrine homeostasis, in transport and storage of essential metals required for fetal organogenesis, as well as in the protection of fetus against bacterial toxins.

Published in	American Journal of BioScience (Volume 3, Issue 2-2) This article belongs to the Special Issue Oxidants-Antioxidants, The Biological Balance
DOI	10.11648/j.ajbio.s.2015030202.12
Page(s)	1-7
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Metallothionein I/II, Peptidoglycan Monomer Linked with Zinc, Syngeneic Pregnancy, Feto-Placental Unit, Fetal Organogenesis

References

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Hrvoje Jakovac, Damir Grebić, Ines Mrakovcic-Šutić, Daniel Rukavina, Biserka Radošević-Stašić. (2015). Expression of Metallothioneins in Placental and Fetal Tissues in Undisturbed and PGM-Zn Treated Syngeneic Pregnancy. American Journal of BioScience, 3(2-2), 1-7. https://doi.org/10.11648/j.ajbio.s.2015030202.12

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Hrvoje Jakovac; Damir Grebić; Ines Mrakovcic-Šutić; Daniel Rukavina; Biserka Radošević-Stašić. Expression of Metallothioneins in Placental and Fetal Tissues in Undisturbed and PGM-Zn Treated Syngeneic Pregnancy. Am. J. BioScience 2015, 3(2-2), 1-7. doi: 10.11648/j.ajbio.s.2015030202.12

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Hrvoje Jakovac, Damir Grebić, Ines Mrakovcic-Šutić, Daniel Rukavina, Biserka Radošević-Stašić. Expression of Metallothioneins in Placental and Fetal Tissues in Undisturbed and PGM-Zn Treated Syngeneic Pregnancy. Am J BioScience. 2015;3(2-2):1-7. doi: 10.11648/j.ajbio.s.2015030202.12

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@article{10.11648/j.ajbio.s.2015030202.12,
  author = {Hrvoje Jakovac and Damir Grebić and Ines Mrakovcic-Šutić and Daniel Rukavina and Biserka Radošević-Stašić},
  title = {Expression of Metallothioneins in Placental and Fetal Tissues in Undisturbed and PGM-Zn Treated Syngeneic Pregnancy},
  journal = {American Journal of BioScience},
  volume = {3},
  number = {2-2},
  pages = {1-7},
  doi = {10.11648/j.ajbio.s.2015030202.12},
  url = {https://doi.org/10.11648/j.ajbio.s.2015030202.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.s.2015030202.12},
  abstract = {Metallothioneins (MTs) are cysteine-rich proteins, which have been implicated in regulation of physiological processes, such as cell growth, repair, differentiation, apoptosis and immunoregulation, as well as in the protection against heavy metals, oxidant damages, inflammation and other stressful conditions. To investigate their roles in physiological and detoxification processes at the maternal-fetal interface and in fetal organogenesis we examined the tissue expression of MT I/II isoforms in undisturbed syngeneic pregnancy and after the treatment with peptidoglycan monomer linked with zinc (PGM-Zn). The data showed that in undisturbed pregnancy the MTs were highly expressed at junctional zone of placenta, on endovascular trophoblast giant cells, glycogen cells and spongiotrophoblast cells, as well as on villous trophoblast cells. In the fetus they were found predominantly in the liver and in epithelial tissues, such as intestine, pancreas and kidney. Treatment with PGM-Zn markedly enhanced the intensity of MT staining both at the maternal-fetal interface and in fetal organs. The data imply that MTs are involved in the protection of trophoblast cells against the pregnancy-induced deregulation of redox and neuro-immuno-endocrine homeostasis, in transport and storage of essential metals required for fetal organogenesis, as well as in the protection of fetus against bacterial toxins.},
 year = {2015}
}

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TY  - JOUR
T1  - Expression of Metallothioneins in Placental and Fetal Tissues in Undisturbed and PGM-Zn Treated Syngeneic Pregnancy
AU  - Hrvoje Jakovac
AU  - Damir Grebić
AU  - Ines Mrakovcic-Šutić
AU  - Daniel Rukavina
AU  - Biserka Radošević-Stašić
Y1  - 2015/03/02
PY  - 2015
N1  - https://doi.org/10.11648/j.ajbio.s.2015030202.12
DO  - 10.11648/j.ajbio.s.2015030202.12
T2  - American Journal of BioScience
JF  - American Journal of BioScience
JO  - American Journal of BioScience
SP  - 1
EP  - 7
PB  - Science Publishing Group
SN  - 2330-0167
UR  - https://doi.org/10.11648/j.ajbio.s.2015030202.12
AB  - Metallothioneins (MTs) are cysteine-rich proteins, which have been implicated in regulation of physiological processes, such as cell growth, repair, differentiation, apoptosis and immunoregulation, as well as in the protection against heavy metals, oxidant damages, inflammation and other stressful conditions. To investigate their roles in physiological and detoxification processes at the maternal-fetal interface and in fetal organogenesis we examined the tissue expression of MT I/II isoforms in undisturbed syngeneic pregnancy and after the treatment with peptidoglycan monomer linked with zinc (PGM-Zn). The data showed that in undisturbed pregnancy the MTs were highly expressed at junctional zone of placenta, on endovascular trophoblast giant cells, glycogen cells and spongiotrophoblast cells, as well as on villous trophoblast cells. In the fetus they were found predominantly in the liver and in epithelial tissues, such as intestine, pancreas and kidney. Treatment with PGM-Zn markedly enhanced the intensity of MT staining both at the maternal-fetal interface and in fetal organs. The data imply that MTs are involved in the protection of trophoblast cells against the pregnancy-induced deregulation of redox and neuro-immuno-endocrine homeostasis, in transport and storage of essential metals required for fetal organogenesis, as well as in the protection of fetus against bacterial toxins.
VL  - 3
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Author Information

Hrvoje Jakovac

Department of Physiology and Immunology, Medical Faculty, University of Rijeka, Rijeka, Croatia
Damir Grebić
Ines Mrakovcic-Šutić
Daniel Rukavina

Croatian Academy of Sciences and Arts, Department of Clinical and Transplantation Immunology and Molecular Medicine, University of Rijeka, Rijeka, Croatia
Biserka Radošević-Stašić

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Hrvoje Jakovac, Damir Grebić, Ines Mrakovcic-Šutić, Daniel Rukavina, Biserka Radošević-Stašić. (2015). Expression of Metallothioneins in Placental and Fetal Tissues in Undisturbed and PGM-Zn Treated Syngeneic Pregnancy. American Journal of BioScience, 3(2-2), 1-7. https://doi.org/10.11648/j.ajbio.s.2015030202.12

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Hrvoje Jakovac; Damir Grebić; Ines Mrakovcic-Šutić; Daniel Rukavina; Biserka Radošević-Stašić. Expression of Metallothioneins in Placental and Fetal Tissues in Undisturbed and PGM-Zn Treated Syngeneic Pregnancy. Am. J. BioScience 2015, 3(2-2), 1-7. doi: 10.11648/j.ajbio.s.2015030202.12

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Hrvoje Jakovac, Damir Grebić, Ines Mrakovcic-Šutić, Daniel Rukavina, Biserka Radošević-Stašić. Expression of Metallothioneins in Placental and Fetal Tissues in Undisturbed and PGM-Zn Treated Syngeneic Pregnancy. Am J BioScience. 2015;3(2-2):1-7. doi: 10.11648/j.ajbio.s.2015030202.12

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@article{10.11648/j.ajbio.s.2015030202.12,
  author = {Hrvoje Jakovac and Damir Grebić and Ines Mrakovcic-Šutić and Daniel Rukavina and Biserka Radošević-Stašić},
  title = {Expression of Metallothioneins in Placental and Fetal Tissues in Undisturbed and PGM-Zn Treated Syngeneic Pregnancy},
  journal = {American Journal of BioScience},
  volume = {3},
  number = {2-2},
  pages = {1-7},
  doi = {10.11648/j.ajbio.s.2015030202.12},
  url = {https://doi.org/10.11648/j.ajbio.s.2015030202.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.s.2015030202.12},
  abstract = {Metallothioneins (MTs) are cysteine-rich proteins, which have been implicated in regulation of physiological processes, such as cell growth, repair, differentiation, apoptosis and immunoregulation, as well as in the protection against heavy metals, oxidant damages, inflammation and other stressful conditions. To investigate their roles in physiological and detoxification processes at the maternal-fetal interface and in fetal organogenesis we examined the tissue expression of MT I/II isoforms in undisturbed syngeneic pregnancy and after the treatment with peptidoglycan monomer linked with zinc (PGM-Zn). The data showed that in undisturbed pregnancy the MTs were highly expressed at junctional zone of placenta, on endovascular trophoblast giant cells, glycogen cells and spongiotrophoblast cells, as well as on villous trophoblast cells. In the fetus they were found predominantly in the liver and in epithelial tissues, such as intestine, pancreas and kidney. Treatment with PGM-Zn markedly enhanced the intensity of MT staining both at the maternal-fetal interface and in fetal organs. The data imply that MTs are involved in the protection of trophoblast cells against the pregnancy-induced deregulation of redox and neuro-immuno-endocrine homeostasis, in transport and storage of essential metals required for fetal organogenesis, as well as in the protection of fetus against bacterial toxins.},
 year = {2015}
}

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TY  - JOUR
T1  - Expression of Metallothioneins in Placental and Fetal Tissues in Undisturbed and PGM-Zn Treated Syngeneic Pregnancy
AU  - Hrvoje Jakovac
AU  - Damir Grebić
AU  - Ines Mrakovcic-Šutić
AU  - Daniel Rukavina
AU  - Biserka Radošević-Stašić
Y1  - 2015/03/02
PY  - 2015
N1  - https://doi.org/10.11648/j.ajbio.s.2015030202.12
DO  - 10.11648/j.ajbio.s.2015030202.12
T2  - American Journal of BioScience
JF  - American Journal of BioScience
JO  - American Journal of BioScience
SP  - 1
EP  - 7
PB  - Science Publishing Group
SN  - 2330-0167
UR  - https://doi.org/10.11648/j.ajbio.s.2015030202.12
AB  - Metallothioneins (MTs) are cysteine-rich proteins, which have been implicated in regulation of physiological processes, such as cell growth, repair, differentiation, apoptosis and immunoregulation, as well as in the protection against heavy metals, oxidant damages, inflammation and other stressful conditions. To investigate their roles in physiological and detoxification processes at the maternal-fetal interface and in fetal organogenesis we examined the tissue expression of MT I/II isoforms in undisturbed syngeneic pregnancy and after the treatment with peptidoglycan monomer linked with zinc (PGM-Zn). The data showed that in undisturbed pregnancy the MTs were highly expressed at junctional zone of placenta, on endovascular trophoblast giant cells, glycogen cells and spongiotrophoblast cells, as well as on villous trophoblast cells. In the fetus they were found predominantly in the liver and in epithelial tissues, such as intestine, pancreas and kidney. Treatment with PGM-Zn markedly enhanced the intensity of MT staining both at the maternal-fetal interface and in fetal organs. The data imply that MTs are involved in the protection of trophoblast cells against the pregnancy-induced deregulation of redox and neuro-immuno-endocrine homeostasis, in transport and storage of essential metals required for fetal organogenesis, as well as in the protection of fetus against bacterial toxins.
VL  - 3
IS  - 2-2
ER  -

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