Pyrin Gene Polymorphisms and <i>H. pylori</i>-associated Dyspepsia: A Sri Lankan Study

Yashodha Weerasinghe; Chinthika Gunasekara; Manjula Weerasekera; Surangi Jayakody; Bimalka Seneviratne; Deepaka Weerasekara; Chaturika Jayasinghe; Navoda Perera; Thilini Gamage; Neluka Fernando

doi:doi:10.11648/j.ajlm.20210601.11

Pyrin Gene Polymorphisms and H. pylori-associated Dyspepsia: A Sri Lankan Study

Yashodha Weerasinghe, Chinthika Gunasekara, Manjula Weerasekera, Surangi Jayakody, Bimalka Seneviratne, Deepaka Weerasekara, Chaturika Jayasinghe, Navoda Perera, Thilini Gamage, Neluka Fernando

Published in American Journal of Laboratory Medicine (Volume 6, Issue 1)

Received: 20 December 2020 Accepted: 11 January 2021 Published: 22 January 2021

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Abstract

Background/Aim: A considerable high number of dyspeptic patients were reported even with the decreasing prevalence of H. pylori in Sri Lanka. Several microbial, host, and environmental factors may associate with the disease outcome. Pyrin secreted by the white blood cells may modulate the inflammatory process by assembling inflammasome complexes in response to pathogen infection. This study focused on the role of pyrin gene polymorphism in gastric mucosal severity and H. pylori infection. Materials and Methods: Among the ninety dyspeptic patients three gastric biopsies were taken and the presence of H. pylori, yeast species and the gastric mucosal severity was determined. EDTA blood was used for DNA extraction and identification of pyrin gene polymorphism. 12 MEFV gene mutations were tested. Results: Most of the patients (61%) had mild chronic gastritis. Among them 11.1% specimens gave positive bands for NL1/LS2 PCR of yeast DNA. H. pylori was positive in 17 patients. No homozygous mutations were found in the MEFV gene. The most common three heterozygous mutations were E148Q (45%), P369S (5%), M680I (11.6%). No significant difference was observed between the presence of the gene polymorphism, gastric mucosal severity or the presence of H. pylori and yeast species in the study group. Conclusion: The absence of homozygous mutations in the MEFV gene suggests that it is not a main factor contributing to gastric mucosal severity. The presence of H. pylori and yeasts reinforce the concept that stomach is a non-sterile environment.

Published in	American Journal of Laboratory Medicine (Volume 6, Issue 1)
DOI	10.11648/j.ajlm.20210601.11
Page(s)	1-7
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

H. pylori, Gut Microbiota, Pyrin

References

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APA Style

Yashodha Weerasinghe, Chinthika Gunasekara, Manjula Weerasekera, Surangi Jayakody, Bimalka Seneviratne, et al. (2021). Pyrin Gene Polymorphisms and H. pylori-associated Dyspepsia: A Sri Lankan Study. American Journal of Laboratory Medicine, 6(1), 1-7. https://doi.org/10.11648/j.ajlm.20210601.11

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ACS Style

Yashodha Weerasinghe; Chinthika Gunasekara; Manjula Weerasekera; Surangi Jayakody; Bimalka Seneviratne, et al. Pyrin Gene Polymorphisms and H. pylori-associated Dyspepsia: A Sri Lankan Study. Am. J. Lab. Med. 2021, 6(1), 1-7. doi: 10.11648/j.ajlm.20210601.11

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AMA Style

Yashodha Weerasinghe, Chinthika Gunasekara, Manjula Weerasekera, Surangi Jayakody, Bimalka Seneviratne, et al. Pyrin Gene Polymorphisms and H. pylori-associated Dyspepsia: A Sri Lankan Study. Am J Lab Med. 2021;6(1):1-7. doi: 10.11648/j.ajlm.20210601.11

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@article{10.11648/j.ajlm.20210601.11,
  author = {Yashodha Weerasinghe and Chinthika Gunasekara and Manjula Weerasekera and Surangi Jayakody and Bimalka Seneviratne and Deepaka Weerasekara and Chaturika Jayasinghe and Navoda Perera and Thilini Gamage and Neluka Fernando},
  title = {Pyrin Gene Polymorphisms and H. pylori-associated Dyspepsia: A Sri Lankan Study},
  journal = {American Journal of Laboratory Medicine},
  volume = {6},
  number = {1},
  pages = {1-7},
  doi = {10.11648/j.ajlm.20210601.11},
  url = {https://doi.org/10.11648/j.ajlm.20210601.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajlm.20210601.11},
  abstract = {Background/Aim: A considerable high number of dyspeptic patients were reported even with the decreasing prevalence of H. pylori in Sri Lanka. Several microbial, host, and environmental factors may associate with the disease outcome. Pyrin secreted by the white blood cells may modulate the inflammatory process by assembling inflammasome complexes in response to pathogen infection. This study focused on the role of pyrin gene polymorphism in gastric mucosal severity and H. pylori infection. Materials and Methods: Among the ninety dyspeptic patients three gastric biopsies were taken and the presence of H. pylori, yeast species and the gastric mucosal severity was determined. EDTA blood was used for DNA extraction and identification of pyrin gene polymorphism. 12 MEFV gene mutations were tested. Results: Most of the patients (61%) had mild chronic gastritis. Among them 11.1% specimens gave positive bands for NL1/LS2 PCR of yeast DNA. H. pylori was positive in 17 patients. No homozygous mutations were found in the MEFV gene. The most common three heterozygous mutations were E148Q (45%), P369S (5%), M680I (11.6%). No significant difference was observed between the presence of the gene polymorphism, gastric mucosal severity or the presence of H. pylori and yeast species in the study group. Conclusion: The absence of homozygous mutations in the MEFV gene suggests that it is not a main factor contributing to gastric mucosal severity. The presence of H. pylori and yeasts reinforce the concept that stomach is a non-sterile environment.},
 year = {2021}
}

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TY - JOUR
T1 - Pyrin Gene Polymorphisms and H. pylori-associated Dyspepsia: A Sri Lankan Study
AU - Yashodha Weerasinghe
AU - Chinthika Gunasekara
AU - Manjula Weerasekera
AU - Surangi Jayakody
AU - Bimalka Seneviratne
AU - Deepaka Weerasekara
AU - Chaturika Jayasinghe
AU - Navoda Perera
AU - Thilini Gamage
AU - Neluka Fernando
Y1 - 2021/01/22
PY - 2021
N1 - https://doi.org/10.11648/j.ajlm.20210601.11
DO - 10.11648/j.ajlm.20210601.11
T2 - American Journal of Laboratory Medicine
JF - American Journal of Laboratory Medicine
JO - American Journal of Laboratory Medicine
SP - 1
EP - 7
PB - Science Publishing Group
SN - 2575-386X
UR - https://doi.org/10.11648/j.ajlm.20210601.11
AB - Background/Aim: A considerable high number of dyspeptic patients were reported even with the decreasing prevalence of H. pylori in Sri Lanka. Several microbial, host, and environmental factors may associate with the disease outcome. Pyrin secreted by the white blood cells may modulate the inflammatory process by assembling inflammasome complexes in response to pathogen infection. This study focused on the role of pyrin gene polymorphism in gastric mucosal severity and H. pylori infection. Materials and Methods: Among the ninety dyspeptic patients three gastric biopsies were taken and the presence of H. pylori, yeast species and the gastric mucosal severity was determined. EDTA blood was used for DNA extraction and identification of pyrin gene polymorphism. 12 MEFV gene mutations were tested. Results: Most of the patients (61%) had mild chronic gastritis. Among them 11.1% specimens gave positive bands for NL1/LS2 PCR of yeast DNA. H. pylori was positive in 17 patients. No homozygous mutations were found in the MEFV gene. The most common three heterozygous mutations were E148Q (45%), P369S (5%), M680I (11.6%). No significant difference was observed between the presence of the gene polymorphism, gastric mucosal severity or the presence of H. pylori and yeast species in the study group. Conclusion: The absence of homozygous mutations in the MEFV gene suggests that it is not a main factor contributing to gastric mucosal severity. The presence of H. pylori and yeasts reinforce the concept that stomach is a non-sterile environment.
VL - 6
IS - 1
ER -

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Author Information

Yashodha Weerasinghe

Department of Microbiology, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka
Chinthika Gunasekara

Department of Microbiology, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka
Manjula Weerasekera

Department of Microbiology, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka
Surangi Jayakody

Department of Community Medicine, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka
Bimalka Seneviratne

Department of Pathology, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka
Deepaka Weerasekara

Department of Surgery, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka
Chaturika Jayasinghe

Department of Medical Laboratory Sciences, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka
Navoda Perera

Department of Medical Laboratory Sciences, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka
Thilini Gamage

Department of Microbiology, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka
Neluka Fernando

Department of Microbiology, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka

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APA Style

Yashodha Weerasinghe, Chinthika Gunasekara, Manjula Weerasekera, Surangi Jayakody, Bimalka Seneviratne, et al. (2021). Pyrin Gene Polymorphisms and H. pylori-associated Dyspepsia: A Sri Lankan Study. American Journal of Laboratory Medicine, 6(1), 1-7. https://doi.org/10.11648/j.ajlm.20210601.11

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ACS Style

Yashodha Weerasinghe; Chinthika Gunasekara; Manjula Weerasekera; Surangi Jayakody; Bimalka Seneviratne, et al. Pyrin Gene Polymorphisms and H. pylori-associated Dyspepsia: A Sri Lankan Study. Am. J. Lab. Med. 2021, 6(1), 1-7. doi: 10.11648/j.ajlm.20210601.11

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AMA Style

Yashodha Weerasinghe, Chinthika Gunasekara, Manjula Weerasekera, Surangi Jayakody, Bimalka Seneviratne, et al. Pyrin Gene Polymorphisms and H. pylori-associated Dyspepsia: A Sri Lankan Study. Am J Lab Med. 2021;6(1):1-7. doi: 10.11648/j.ajlm.20210601.11

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@article{10.11648/j.ajlm.20210601.11,
  author = {Yashodha Weerasinghe and Chinthika Gunasekara and Manjula Weerasekera and Surangi Jayakody and Bimalka Seneviratne and Deepaka Weerasekara and Chaturika Jayasinghe and Navoda Perera and Thilini Gamage and Neluka Fernando},
  title = {Pyrin Gene Polymorphisms and H. pylori-associated Dyspepsia: A Sri Lankan Study},
  journal = {American Journal of Laboratory Medicine},
  volume = {6},
  number = {1},
  pages = {1-7},
  doi = {10.11648/j.ajlm.20210601.11},
  url = {https://doi.org/10.11648/j.ajlm.20210601.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajlm.20210601.11},
  abstract = {Background/Aim: A considerable high number of dyspeptic patients were reported even with the decreasing prevalence of H. pylori in Sri Lanka. Several microbial, host, and environmental factors may associate with the disease outcome. Pyrin secreted by the white blood cells may modulate the inflammatory process by assembling inflammasome complexes in response to pathogen infection. This study focused on the role of pyrin gene polymorphism in gastric mucosal severity and H. pylori infection. Materials and Methods: Among the ninety dyspeptic patients three gastric biopsies were taken and the presence of H. pylori, yeast species and the gastric mucosal severity was determined. EDTA blood was used for DNA extraction and identification of pyrin gene polymorphism. 12 MEFV gene mutations were tested. Results: Most of the patients (61%) had mild chronic gastritis. Among them 11.1% specimens gave positive bands for NL1/LS2 PCR of yeast DNA. H. pylori was positive in 17 patients. No homozygous mutations were found in the MEFV gene. The most common three heterozygous mutations were E148Q (45%), P369S (5%), M680I (11.6%). No significant difference was observed between the presence of the gene polymorphism, gastric mucosal severity or the presence of H. pylori and yeast species in the study group. Conclusion: The absence of homozygous mutations in the MEFV gene suggests that it is not a main factor contributing to gastric mucosal severity. The presence of H. pylori and yeasts reinforce the concept that stomach is a non-sterile environment.},
 year = {2021}
}

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