Improvement of Phenolic Compounds and Antihyperlipidemic Activity of <i>Hibiscus sabdariffa</i> L. Calyxes Powder Using CDS Processing

Linda Stella Mbassi; Elie Baudelaire Djantou; Richard Marcel Nguimbou; Amadou Dicko; Nicolas Yanou Njintang

doi:doi:10.11648/j.ajls.20231102.13

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Improvement of Phenolic Compounds and Antihyperlipidemic Activity of Hibiscus sabdariffa L. Calyxes Powder Using CDS Processing

Linda Stella Mbassi, Elie Baudelaire Djantou, Richard Marcel Nguimbou, Amadou Dicko, Nicolas Yanou Njintang

Published in American Journal of Life Sciences (Volume 11, Issue 2)

Received: 10 March 2022 Accepted: 11 August 2022 Published: 9 June 2023

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Abstract

The aim of this study was to evaluate the effect of controlled differential sieving processing (CDSp) on the content of phenolic compounds and antihyperlipidemic activity of H. sabdariffa calyxes powder. For this, four granulometric classes (<180 μm, 180-212 μm, 212-315 μm, ≥315 μm) were analyzed. Unsieved powder and lyophilized ethanolic extract were used for comparison. First, we identified and quantified the phenolic compounds present in the samples by chromatography in liquid phase coupled to a UV detector and mass spectrometry (LC-MS). Second, we evaluated the antihyperlipidemic activity of the different fractions in adult male rats at a dose of 250 mg/kg and compared them with those of unsieved powder and the ethanolic extract. LC-MS analysis identified seven compounds (quercetin, rutin, catechin, ferrulic acid, p-coumaric acid, protocatechic acid and cafeic acid) in the different sample of H. sabdariffa calyxes powder. The granulometric class 212-315 μm showed the best concentration, higher than those of the other granulometric class and crude powder. This fraction also showed content of phenolic compounds higher than those of ethanolic extract except for p-coumaric acid, and protocatechic acid which were concentrated equally in both samples. Antihyperlipidemic activity were significantly influenced (P <0.05) by particle sizes. The best activity was obtained in the particle size classes of 212-315 µm and <180 µm which were significantly (P <0.05) higher than those of the other fractions and of the unsieved powder to improve the lipid parameters (total cholesterol, LDL-C, HDL-C and triglycerides), transaminases (ALAT and ASAT) and creatine. However, compared to the ethanolic extract, only the granulometric class of 212-315 µm showed a significantly higher activity. CDSp can be an alternative to ethanol extraction and may be an option to improve the content of phenolic compounds and antihyperlipidemic activity of plant powders.

Published in	American Journal of Life Sciences (Volume 11, Issue 2)
DOI	10.11648/j.ajls.20231102.13
Page(s)	24-35
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Antihyperlipidemic Activity, Hibiscus sabdariffa Calyxes, Powder Fraction, Lyophilized Ethanolic Extract, Phenolic Compounds

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Linda Stella Mbassi, Elie Baudelaire Djantou, Richard Marcel Nguimbou, Amadou Dicko, Nicolas Yanou Njintang. (2023). Improvement of Phenolic Compounds and Antihyperlipidemic Activity of Hibiscus sabdariffa L. Calyxes Powder Using CDS Processing. American Journal of Life Sciences, 11(2), 24-35. https://doi.org/10.11648/j.ajls.20231102.13

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Linda Stella Mbassi; Elie Baudelaire Djantou; Richard Marcel Nguimbou; Amadou Dicko; Nicolas Yanou Njintang. Improvement of Phenolic Compounds and Antihyperlipidemic Activity of Hibiscus sabdariffa L. Calyxes Powder Using CDS Processing. Am. J. Life Sci. 2023, 11(2), 24-35. doi: 10.11648/j.ajls.20231102.13

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AMA Style

Linda Stella Mbassi, Elie Baudelaire Djantou, Richard Marcel Nguimbou, Amadou Dicko, Nicolas Yanou Njintang. Improvement of Phenolic Compounds and Antihyperlipidemic Activity of Hibiscus sabdariffa L. Calyxes Powder Using CDS Processing. Am J Life Sci. 2023;11(2):24-35. doi: 10.11648/j.ajls.20231102.13

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@article{10.11648/j.ajls.20231102.13,
  author = {Linda Stella Mbassi and Elie Baudelaire Djantou and Richard Marcel Nguimbou and Amadou Dicko and Nicolas Yanou Njintang},
  title = {Improvement of Phenolic Compounds and Antihyperlipidemic Activity of Hibiscus sabdariffa L. Calyxes Powder Using CDS Processing},
  journal = {American Journal of Life Sciences},
  volume = {11},
  number = {2},
  pages = {24-35},
  doi = {10.11648/j.ajls.20231102.13},
  url = {https://doi.org/10.11648/j.ajls.20231102.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20231102.13},
  abstract = {The aim of this study was to evaluate the effect of controlled differential sieving processing (CDSp) on the content of phenolic compounds and antihyperlipidemic activity of H. sabdariffa calyxes powder. For this, four granulometric classes (H. sabdariffa calyxes powder. The granulometric class 212-315 μm showed the best concentration, higher than those of the other granulometric class and crude powder. This fraction also showed content of phenolic compounds higher than those of ethanolic extract except for p-coumaric acid, and protocatechic acid which were concentrated equally in both samples. Antihyperlipidemic activity were significantly influenced (P <0.05) by particle sizes. The best activity was obtained in the particle size classes of 212-315 µm and <180 µm which were significantly (P <0.05) higher than those of the other fractions and of the unsieved powder to improve the lipid parameters (total cholesterol, LDL-C, HDL-C and triglycerides), transaminases (ALAT and ASAT) and creatine. However, compared to the ethanolic extract, only the granulometric class of 212-315 µm showed a significantly higher activity. CDSp can be an alternative to ethanol extraction and may be an option to improve the content of phenolic compounds and antihyperlipidemic activity of plant powders.},
 year = {2023}
}

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TY  - JOUR
T1  - Improvement of Phenolic Compounds and Antihyperlipidemic Activity of Hibiscus sabdariffa L. Calyxes Powder Using CDS Processing
AU  - Linda Stella Mbassi
AU  - Elie Baudelaire Djantou
AU  - Richard Marcel Nguimbou
AU  - Amadou Dicko
AU  - Nicolas Yanou Njintang
Y1  - 2023/06/09
PY  - 2023
N1  - https://doi.org/10.11648/j.ajls.20231102.13
DO  - 10.11648/j.ajls.20231102.13
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 24
EP  - 35
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.20231102.13
AB  - The aim of this study was to evaluate the effect of controlled differential sieving processing (CDSp) on the content of phenolic compounds and antihyperlipidemic activity of H. sabdariffa calyxes powder. For this, four granulometric classes (H. sabdariffa calyxes powder. The granulometric class 212-315 μm showed the best concentration, higher than those of the other granulometric class and crude powder. This fraction also showed content of phenolic compounds higher than those of ethanolic extract except for p-coumaric acid, and protocatechic acid which were concentrated equally in both samples. Antihyperlipidemic activity were significantly influenced (P <0.05) by particle sizes. The best activity was obtained in the particle size classes of 212-315 µm and <180 µm which were significantly (P <0.05) higher than those of the other fractions and of the unsieved powder to improve the lipid parameters (total cholesterol, LDL-C, HDL-C and triglycerides), transaminases (ALAT and ASAT) and creatine. However, compared to the ethanolic extract, only the granulometric class of 212-315 µm showed a significantly higher activity. CDSp can be an alternative to ethanol extraction and may be an option to improve the content of phenolic compounds and antihyperlipidemic activity of plant powders.
VL  - 11
IS  - 2
ER  -

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Author Information

Linda Stella Mbassi

Institute of Agricultural Research for Development (IRAD), Ngaoundere, Cameroon
Elie Baudelaire Djantou

Department of Food Sciences and Nutrition, National School of Agro-industrial Science, University of Ngaoundere, Ngaoundere, Cameroon
Richard Marcel Nguimbou

Department of Food Sciences and Nutrition, National School of Agro-industrial Science, University of Ngaoundere, Ngaoundere, Cameroon
Amadou Dicko

University of Lorraine, Chemistry and Physics Laboratory - Multi-Scale Approach to Complex Media, Metz, France
Nicolas Yanou Njintang

Department of Biological Sciences, Faculty of Sciences, University of Ngaoundere, Ngaoundere, Cameroon

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Linda Stella Mbassi, Elie Baudelaire Djantou, Richard Marcel Nguimbou, Amadou Dicko, Nicolas Yanou Njintang. (2023). Improvement of Phenolic Compounds and Antihyperlipidemic Activity of Hibiscus sabdariffa L. Calyxes Powder Using CDS Processing. American Journal of Life Sciences, 11(2), 24-35. https://doi.org/10.11648/j.ajls.20231102.13

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ACS Style

Linda Stella Mbassi; Elie Baudelaire Djantou; Richard Marcel Nguimbou; Amadou Dicko; Nicolas Yanou Njintang. Improvement of Phenolic Compounds and Antihyperlipidemic Activity of Hibiscus sabdariffa L. Calyxes Powder Using CDS Processing. Am. J. Life Sci. 2023, 11(2), 24-35. doi: 10.11648/j.ajls.20231102.13

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AMA Style

Linda Stella Mbassi, Elie Baudelaire Djantou, Richard Marcel Nguimbou, Amadou Dicko, Nicolas Yanou Njintang. Improvement of Phenolic Compounds and Antihyperlipidemic Activity of Hibiscus sabdariffa L. Calyxes Powder Using CDS Processing. Am J Life Sci. 2023;11(2):24-35. doi: 10.11648/j.ajls.20231102.13

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@article{10.11648/j.ajls.20231102.13,
  author = {Linda Stella Mbassi and Elie Baudelaire Djantou and Richard Marcel Nguimbou and Amadou Dicko and Nicolas Yanou Njintang},
  title = {Improvement of Phenolic Compounds and Antihyperlipidemic Activity of Hibiscus sabdariffa L. Calyxes Powder Using CDS Processing},
  journal = {American Journal of Life Sciences},
  volume = {11},
  number = {2},
  pages = {24-35},
  doi = {10.11648/j.ajls.20231102.13},
  url = {https://doi.org/10.11648/j.ajls.20231102.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20231102.13},
  abstract = {The aim of this study was to evaluate the effect of controlled differential sieving processing (CDSp) on the content of phenolic compounds and antihyperlipidemic activity of H. sabdariffa calyxes powder. For this, four granulometric classes (H. sabdariffa calyxes powder. The granulometric class 212-315 μm showed the best concentration, higher than those of the other granulometric class and crude powder. This fraction also showed content of phenolic compounds higher than those of ethanolic extract except for p-coumaric acid, and protocatechic acid which were concentrated equally in both samples. Antihyperlipidemic activity were significantly influenced (P <0.05) by particle sizes. The best activity was obtained in the particle size classes of 212-315 µm and <180 µm which were significantly (P <0.05) higher than those of the other fractions and of the unsieved powder to improve the lipid parameters (total cholesterol, LDL-C, HDL-C and triglycerides), transaminases (ALAT and ASAT) and creatine. However, compared to the ethanolic extract, only the granulometric class of 212-315 µm showed a significantly higher activity. CDSp can be an alternative to ethanol extraction and may be an option to improve the content of phenolic compounds and antihyperlipidemic activity of plant powders.},
 year = {2023}
}

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TY  - JOUR
T1  - Improvement of Phenolic Compounds and Antihyperlipidemic Activity of Hibiscus sabdariffa L. Calyxes Powder Using CDS Processing
AU  - Linda Stella Mbassi
AU  - Elie Baudelaire Djantou
AU  - Richard Marcel Nguimbou
AU  - Amadou Dicko
AU  - Nicolas Yanou Njintang
Y1  - 2023/06/09
PY  - 2023
N1  - https://doi.org/10.11648/j.ajls.20231102.13
DO  - 10.11648/j.ajls.20231102.13
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 24
EP  - 35
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.20231102.13
AB  - The aim of this study was to evaluate the effect of controlled differential sieving processing (CDSp) on the content of phenolic compounds and antihyperlipidemic activity of H. sabdariffa calyxes powder. For this, four granulometric classes (H. sabdariffa calyxes powder. The granulometric class 212-315 μm showed the best concentration, higher than those of the other granulometric class and crude powder. This fraction also showed content of phenolic compounds higher than those of ethanolic extract except for p-coumaric acid, and protocatechic acid which were concentrated equally in both samples. Antihyperlipidemic activity were significantly influenced (P <0.05) by particle sizes. The best activity was obtained in the particle size classes of 212-315 µm and <180 µm which were significantly (P <0.05) higher than those of the other fractions and of the unsieved powder to improve the lipid parameters (total cholesterol, LDL-C, HDL-C and triglycerides), transaminases (ALAT and ASAT) and creatine. However, compared to the ethanolic extract, only the granulometric class of 212-315 µm showed a significantly higher activity. CDSp can be an alternative to ethanol extraction and may be an option to improve the content of phenolic compounds and antihyperlipidemic activity of plant powders.
VL  - 11
IS  - 2
ER  -

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