Osteoarthritis (OA) is a chronic degenerative joint disease with an inflammatory component. It is associated with progressive histological alterations and disabling symptoms. Today, drugs such as glucocorticoids (GCs) and nonsteroidal anti-inflammatory drugs (NSAIDs) are commonly employed for the treatment of osteoarthritis but have serious and life-threatening side effects. The current study aims to evaluate the effects of alcoholic essences of Laurus nobilis L. (AELN) on pro-inflammatory cytokines such as cyclooxygenase-2 (COX-2, isoform), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), interleukin-18 (IL-18), tumor necrosis factor-alpha (TNF-α), and nitric oxide (NO), as well as prostaglandin E2 (PGE2) on inflammatory cells, similar osteoarthritis in synoviocytes, and monocytes/ macrophages, and to compare it with dexamethasone (DEX) and ibuprofen (IBP). After collecting the leaves of the Laurus nobilis L. (LN) and after drying, the essences were collected by the Center for Genetic and Biological Resources of Iran. Synovial cells were isolated from the synovial membrane of the radiocarpal joint cartilage of an 8-month-old Holstein cow. THP-1 cells were prepared from the Pasteur Institute of Iran. Cells were cultivated and exposed to lipopolysaccharide (LPS) stimulation without, or in the presence of, DEX, IBP, or alcoholic essences of Laurus nobilis L. (AELN) The gene expressions of IL-1β, TNF-α, IL-18, COX-2, and iNOS were evaluated by real-time PCR. Concentrations of NO and PGE2 were measured by ELISA methods. Treatment of the studied cell with alcoholic essences of Laurus nobilis L, before stimulation with lipopolysaccharide, reduces the expression of proinflammatory cytokine genes such as cyclooxygenase-2, nitric oxide synthase, interleukin-6, interleukin-1 beta, interleukin-18, tumor necrosis factor-alpha, and It also reduces the production of nitric oxide and prostaglandin E2 by almost 50%. This reduction is significant compared to the 90% reduction due to treatment with dexamethasone and ibuprofen. Significant reduction in the expression of pro-inflammatory cytokines by alcoholic essences of Laurus nobilis L can be considered as a new drug in the treatment of osteoarthritis and requires further studies in laboratory animals and clinical studies.
| Published in | Biomedical Sciences (Volume 8, Issue 1) |
| DOI | 10.11648/j.bs.20220801.13 |
| Page(s) | 10-19 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Osteoarthritis, Laurus nobilis L., Synoviocytes, Monocytes/Macrophages, Proinflammatory Cytokine
| [1] | Chow YY, Chin KY. The role of inflammation in the pathogenesis of osteoarthritis. Mediators of inflammation. 2020 Mar 3; 2020. DOI: https://doi.org/10.1155/2020/8293921. |
| [2] | V. B. Kraus, F. J. Blanco, M. Englund, M. A. Karsdal, and L. S. Lohmander, “Call for standardized definitions of osteoarthritis and risk stratification for clinical trials and clinical use,” Osteoarthritis and Cartilage, vol. 23, no. 8, pp. 1233–1241, 2015. DOI: https://doi.org/10.1016/j.joca.2015.03.036. |
| [3] | Kotti, L. D. Duffell, A. A. Faisal, and A. H. McGregor, “The complexity of human walking: a knee osteoarthritis study,” PloS One, vol. 9, no. 9, article e107325, 2014. DOI: https://doi.org/10.1371/journal.pone.0107325. |
| [4] | Tehrani‐Banihashemi A, Davatchi F, Jamshidi AR, Faezi T, Paragomi P, Barghamdi M. Prevalence of osteoarthritis in rural areas of I ran: a WHO‐ILAR COPCORD study. International journal of rheumatic diseases. 2014 May; 17 (4): 384-8. DOI: https://doi.org/10.1111/1756-185X.12312. |
| [5] | Mendy A, Park J, Vieira ER. Osteoarthritis and risk of mortality in the USA: a population-based cohort study. International journal of epidemiology. 2018 Dec 1; 47 (6): 1821-9. DOI: https://doi.org/10.1093/ije/dyy187. |
| [6] | Scherer HU, Häupl T, Burmester GR. The etiology of rheumatoid arthritis. Journal of autoimmunity. 2020 Jun 1; 110: 102400. DOI: https://doi.org/10.1016/j.jaut.2019.102400. |
| [7] | Anderson AS, Loeser RF. Why is osteoarthritis an age-related disease? Best practice & research Clinical rheumatology. 2010 Feb 1; 24 (1): 15-26. DOI: https://doi.org/10.1016/j.berh.2009.08.006. |
| [8] | Goldring MB, Goldring SR. Articular cartilage and subchondral bone in the pathogenesis of osteoarthritis. Annals of the New York Academy of Sciences. 2010 Apr; 1192 (1): 230-7. DOI: https://doi.org/10.1111/j.1749-6632.2009.05240.x. |
| [9] | Abramoff B, Caldera FE. Osteoarthritis: pathology, diagnosis, and treatment options. Medical Clinics. 2020 Mar 1; 104 (2): 293-311. DOI: 10.1016/j.mcna.2019.10.007. |
| [10] | Scanzello CR, Goldring SR. The role of synovitis in osteoarthritis pathogenesis. Bone. 2012 Aug; 51 (2): 249-57. DOI: 10.1016/j.bone.2012.02.012. |
| [11] | Hou, SM., Hou, CH. & Liu, JF. CX3CL1 promotes MMP-3 production via the CX3CR1, c-Raf, MEK, ERK, and NF-κB signaling pathways in osteoarthritis synovial fibroblasts. Arthritis Res Ther 19, 282 (2017). DOI: https://doi.org/10.1186/s13075-017-1487-6. |
| [12] | Ioan-Facsinay, A., Kloppenburg, M. An emerging player in knee osteoarthritis: the infrapatellar fat pad. Arthritis Res Ther 15, 225 (2013). DOI: https://doi.org/10.1186/ar4422. |
| [13] | Goldring, Mary B.; Otero, Miguel Inflammation in osteoarthritis, Current Opinion in Rheumatology: September 2011 - Volume 23 - Issue 5 - p 471-478. http://dx.doi.org/10.1097/BOR.0b013e328349c2b1. |
| [14] | Tecchio C, Micheletti A, Cassatella MA. Neutrophil-derived cytokines: facts beyond expression. Frontiers in Immunology. 2014; 5: 508. DOI: 10.3389/fimmu.2014.00508. |
| [15] | Thomson A, Hilkens CM. Synovial macrophages in osteoarthritis: the key to understanding pathogenesis? Frontiers in Immunology. 2021; 12. DOI: 10.3389/fimmu.2021.678757. |
| [16] | Sellam J, Berenbaum F. The role of synovitis in pathophysiology and clinical symptoms of osteoarthritis. Nat Rev Rheumatol. 2010 Nov; 6 (11): 625-35. DOI: 10.1038/nrrheum.2010.159. |
| [17] | Wang Y, Che M, Xin J, Zheng Z, Li J, Zhang S. The role of IL-1β and TNF-α in intervertebral disc degeneration. Biomedicine & Pharmacotherapy. 2020 Nov 1; 131: 110660. DOI: https://doi.org/10.1016/j.biopha.2020.110660. |
| [18] | Deligne C, Casulli S, Pigment A, Bougault C, Campillo-Gimenez L, Nourissat G, Berenbaum F, Elbim C, Houard X. Differential expression of interleukin-17 and interleukin-22 in inflamed and non-inflamed synovium from osteoarthritis patients. Osteoarthritis and cartilage. 2015 Nov 1; 23 (11): 1843-52. DOI: https://doi.org/10.1016/j.joca.2014.12.007. |
| [19] | Li Y, Zhou Y. Interleukin-17: the role for pathological angiogenesis in ocular neovascular diseases. The Tohoku journal of experimental medicine. 2019; 247 (2): 87-98. DOI: 10.1620/tjem.247.87. |
| [20] | Monasterio G, Castillo F, Betancur D, Hernández A, Flores G, Díaz W, Hernández M, Vernal R. Osteoarthritis of the temporomandibular joint: clinical and immunological diagnosis, the pathogenic role of the immuno-inflammatory response, and immunotherapeutic strategies based on T regulatory lymphocytes. In Temporomandibular Joint Pathology-Current Approaches and Understanding 2018 Feb 28. IntechOpen. DOI: 10.5772/intechopen.72496. |
| [21] | Carrión M, Juarranz Y, Seoane IV, Martínez C, González-Álvaro I, Pablos JL, Gutiérrez-Cañas I, Gomariz RP. VIP modulates IL-22R1 expression and prevents the contribution of rheumatoid synovial fibroblasts to IL-22-mediated joint destruction. Journal of Molecular Neuroscience. 2014 Jan; 52 (1): 10-7. DOI: https://doi.org/10.1007/s12031-013-0177-3 |
| [22] | Fernando MR, Reyes JL, Iannuzzi J, Leung G, McKay DM. The pro-inflammatory cytokine, interleukin-6, enhances the polarization of alternatively activated macrophages. PloS one. 2014 Apr 15; 9 (4): e94188. DOI: https://doi.org/10.1371/journal.pone.0094188. |
| [23] | Yujie Sun, Lugang Zhou, Dongmei Lv, Hongzhi Liu, Tian He, Xin Wang, Poly (ADP-ribose) polymerase 1 inhibition prevents interleukin-1β-induced inflammation in human osteoarthritic chondrocytes, Acta Biochimica et Biophysica Sinica, Volume 47, Issue 6, June 2015, Pages 422–430, DOI: https://doi.org/10.1093/abbs/gmv033. |
| [24] | Ma C, Zhang Y, Li YQ, Chen C, Cai W, Zeng YL. The role of PPARγ in advanced glycation end product-induced inflammatory response in human chondrocytes. PloS one. 2015 May 29; 10 (5): e0125776. DOI: 10.1371/journal.pone.0125776. |
| [25] | Shan Y, Qi C, Liu Y, Gao H, Zhao D, Jiang Y. Increased frequency of peripheral blood follicular helper T cells and elevated serum IL‑21 levels in patients with knee osteoarthritis. Molecular medicine reports. 2017 Mar 1; 15 (3): 1095-102. DOI: https://doi.org/10.3892/mmr.2017.6132. |
| [26] | Chahal KK, Kaur M, Bhardwaj U, Singla N, Kaur A, Kaur M, Bhardwaj U, Singla N, Kaur A. A review on the chemistry and biological activities of Laurus nobilis L. alcoholic essences. Journal of Pharmacognosy and Phytochemistry. 2017; 6 (4): 1153-61. |
| [27] | Caputo L, Nazzaro F, Souza LF, Aliberti L, De Martino L, Fratianni F, Coppola R, De Feo V. Laurus nobilis: Composition of alcoholic essences and its biological activities. Molecules. 2017 Jun; 22 (6): 930. https://doi.org/10.3390/molecules22060930. |
| [28] | Sokolove J, Lepus CM. Role of inflammation in the pathogenesis of osteoarthritis: latest findings and interpretations. Ther Adv Musculoskelet Dis. 2013 Apr; 5 (2): 77-94. Doi: 10.1177/1759720X12467868. |
| [29] | Maghsoudi H, Hallajzadeh J, Rezaeipour M. Evaluation of the effect of polyphenol of escin compared with ibuprofen and dexamethasone in synoviocyte model for osteoarthritis: an in vitro study. Clinical rheumatology. 2018 Sep; 37 (9): 2471-8. DOI: https://doi.org/10.1007/s10067-018-4097-z. |
| [30] | Eriksson JE, Dechat T, Grin B, Helfand B, Mendez M, Pallari HM, Goldman RD. Introducing intermediate filaments: from discovery to disease. The Journal of clinical investigation. 2009 Jul 1; 119 (7): 1763-71. DOI: 10.1172/JCI38339. |
| [31] | Richter E, Ventz K, Harms M, Mostert J and Hochgräfe F. Induction of Macrophage Function in Human THP-1 Cells has Associated with Rewiring of MAPK Signaling and Activation of MAP3K7 (TAK1) Protein Kinase. Front. Cell Dev. Biol. (2016) 4: 21. DOI: https://doi.org/10.3389/fcell.2016.00021. |
| [32] | Jeong, Y. H., Oh, Y. C., Cho, W. K., Shin, H., Lee, K. Y., & Ma, J. Y. (2016). Anti-inflammatory effects of Viola yedoensis and the application of cell extraction methods for investigating bioactive constituents in macrophages. BMC complementary and alternative medicine, 16 (1), 180. DOI: 10.1186/s12906-016-1142-9. |
| [33] | Padumadasa, C., Dharmadana, D., Abeysekera, A., & Thammitiyagodage, M. (2016). In vitro antioxidant, anti-inflammatory and anticancer activities of ethyl acetate soluble proanthocyanidins of the inflorescence of Cocos nucifera L. BMC complementary and alternative medicine, 16 (1), 345. DOI: 10.1186/s12906-016-1335-2. |
| [34] | Garbison KE, Heinz BA, Lajiness ME, Weidner JR, Sittampalam GS, Lajiness ME, Protocol SP, Lamore SD, Scott CW, Peters MF, Axelsson H. Assay Guidance Manual. Eli Lilly & Company and the National Center for Advancing Translational Sciences. 2004. |
| [35] | Nitrite/Nitrate Assay Kit, colorimetric Used for detection of nitric oxide metabolite. Sigma-Aldrich, Catalog Number 23479. https://www.sigmaaldrich.com/US/en/product/sigma/mak367. |
| [36] | Latorre Uriza C, Velosa-Porras J, Roa NS, Quiñones Lara SM, Silva J, Ruiz AJ, Escobar Arregoces FM. Periodontal disease, inflammatory cytokines, and PGE2 in pregnant patients at risk of preterm delivery: a pilot study. Infectious diseases in obstetrics and gynecology. 2018 Aug 1; 2018. DOI: https://doi.org/10.1155/2018/7027683. |
| [37] | Maghsoudi H, Haj-allahyari S. Anti-inflammatory Effect of Alcoholic Extract of Nigella sativa L on Bovine Fibroblast-like synoviocyte and THP-1. International Journal of Contemporary Research and Review. 2018 Feb 17; 9 (02): 20181-91. DOI: https://doi.org/10.15520/ijcrr/2018/9/02/428. |
| [38] | Özcan M, Chalchat JC. Effect of different locations on the chemical composition of alcoholic essencess of laurel (Laurus nobilis L.) leaves growing wild in Turkey. Journal of Medicinal food. 2005 Sep 1; 8 (3): 408-11. 10.1089/jmf.2005.8.408. |
| [39] | Cherrat L, Espina L, Bakkali M, García-Gonzalo D, Pagán R, Laglaoui A. Chemical composition and antioxidant properties of Laurus nobilis L. and Myrtus communis L. alcoholic essences from Morocco and evaluation of their antimicrobial activity acting alone or in combined processes for food preservation. Journal of the Science of Food and Agriculture. 2014 Apr; 94 (6): 1197-204. https://doi.org/10.1002/jsfa.6397. |
| [40] | Li Y, Lai Y, Wang Y, Liu N, Zhang F, Xu P. 1, 8-Cineol protect against influenza-virus-induced pneumonia in mice. Inflammation. 2016 Aug; 39 (4): 1582-93. https://doi.org/10.1007/s10753-016-0394-3. |
| [41] | Lima PR, de Melo TS, Carvalho KM, de Oliveira ÍB, Arruda BR, de Castro Brito GA, Rao VS, Santos FA. 1, 8-cineole (eucalyptol) ameliorates cerulein-induced acute pancreatitis via modulation of cytokines, oxidative stress, and NF-κB activity in mice. Life Sciences. 2013 Jul 10; 92 (24-26): 1195-201. https://doi.org/10.1016/j.lfs.2013.05.009. |
| [42] | Zheng W, Wang SY. Antioxidant activity and phenolic compounds in selected herbs. Journal of Agricultural and Food chemistry. 2001 Nov 19; 49 (11): 5165-70. DOI: https://doi.org/10.1021/jf010697n. |
| [43] | Craig WJ. Health-promoting properties of common herbs. The American journal of clinical nutrition. 1999 Sep 1; 70 (3): 491s-9s. DOI: https://doi.org/10.1093/ajcn/70.3.491s. |
| [44] | Kuo PC, Schroeder RA. The emerging multifaceted roles of nitric oxide. Annals of surgery. 1995 Mar; 221 (3): 220. DOI: 10.1097/00000658-199503000-00003. |
| [45] | Dubois RN, Abramson SB, Crofford L, Gupta RA, Simon LS, Van De Putte LB, Lipsky PE. Cyclooxygenase in biology and disease. The FASEB journal. 1998 Sep; 12 (12): 1063-73. DOI: https://doi.org/10.1096/fasebj.12.12.1063. |
| [46] | Ahmadi A. Synthesis and anti-inflammatory evaluation of novel thiadiazol derivatives of Mefenamic acid. Bulletin of the Chemical Society of Ethiopia. 2017 Jul 19; 31 (1): 171-5. DOI: 10.4314/bcse.v31i1.15. |
| [47] | Ferrero-Miliani L, Nielsen OH, Andersen PS, Girardin S. Chronic inflammation: the importance of NOD2 and NALP3 in an interleukin-1β generation. Clinical & Experimental Immunology. 2007 Feb; 147 (2): 227-35. DOI: DOI: https://doi.org/10.1111/j.1365-2249.2006.03261.x. |
| [48] | Raymuev KV. Pro-inflammatory and anti-inflammatory cytokines in the pathogenesis of osteoarthritis. Herald of North-Western State Medical University named after II Mechnikov. 2018 Nov 19; 10 (3): 19-27. DOI: https://doi.org/10.17816/mechnikov201810319-27. |
| [49] | Kulkarni P, Martson A, Vidya R, Chitnavis S, Harsulkar A. Pathophysiological landscape of osteoarthritis. Advances in Clinical Chemistry. 2021 Jan 1; 100: 37-90. DOI: https://doi.org/10.1016/bs.acc.2020.04.002. |
| [50] | Orrem HL, Shetelig C, Ueland T, Limalanathan S, Nilsson PH, Husebye T, Aukrust P, Seljeflot I, Hoffmann P, Eritsland J, Mollnes TE. Soluble IL-1 receptor 2 is associated with the left ventricular remodeling in patients with ST-elevation myocardial infarction. International journal of cardiology. 2018 Oct 1; 268: 187-92. DOI: https://doi.org/10.1016/j.ijcard.2018.05.032. |
| [51] | Wojdasiewicz P, Poniatowski ŁA, Nauman P, Mandat T, Paradowska-Gorycka A, Romanowska-Próchnicka K, Szukiewicz D, Kotela A, Kubaszewski Ł, Kotela I, Kurkowska-Jastrzębska I. Cytokines in the pathogenesis of hemophilic arthropathy. Cytokine & growth factor reviews. 2018 Feb 1; 39: 71-91. DOI: https://doi.org/10.1016/j.cytogfr.2017.11.003. |
| [52] | Kumar A, Arshad M, Singh A, Khan H, Swaroop S. Association of Cytokine TNF-α in Development of Osteoarthritis: A Comprehensive Study. Journal of Ecophysiology and Occupational Health. 2018 Dec 1; 18 (3-4): 117-21. DOI: https://doi.org/10.18311/jeoh/2018/21447. |
| [53] | Ferrer MD, Busquets-Cortés C, Capo X, Tejada S, Tur JA, Pons A, Sureda A. Cyclooxygenase-2 inhibitors as a therapeutic target in inflammatory diseases. Current medicinal chemistry. 2019 Jun 1; 26 (18): 3225-41. DOI: 10.2174/0929867325666180514112124. |
APA Style
Hossein Maghsoudi, Mahsa Khosrogardi, Amir Akbarnejad Eshkalak, Younes Tatar Mamaghani, Gholamreza Bakhshi Khanaki, et al. (2022). The Effect of the Alcoholic Essence of Laurus nobilis L. on Pro-inflammatoiry Cytokine Gene Expression in Synoviocytes and Macrophage/Monocyte. Biomedical Sciences, 8(1), 10-19. https://doi.org/10.11648/j.bs.20220801.13
ACS Style
Hossein Maghsoudi; Mahsa Khosrogardi; Amir Akbarnejad Eshkalak; Younes Tatar Mamaghani; Gholamreza Bakhshi Khanaki, et al. The Effect of the Alcoholic Essence of Laurus nobilis L. on Pro-inflammatoiry Cytokine Gene Expression in Synoviocytes and Macrophage/Monocyte. Biomed. Sci. 2022, 8(1), 10-19. doi: 10.11648/j.bs.20220801.13
AMA Style
Hossein Maghsoudi, Mahsa Khosrogardi, Amir Akbarnejad Eshkalak, Younes Tatar Mamaghani, Gholamreza Bakhshi Khanaki, et al. The Effect of the Alcoholic Essence of Laurus nobilis L. on Pro-inflammatoiry Cytokine Gene Expression in Synoviocytes and Macrophage/Monocyte. Biomed Sci. 2022;8(1):10-19. doi: 10.11648/j.bs.20220801.13
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author = {Hossein Maghsoudi and Mahsa Khosrogardi and Amir Akbarnejad Eshkalak and Younes Tatar Mamaghani and Gholamreza Bakhshi Khanaki and Enayatollah Yazdanpanah},
title = {The Effect of the Alcoholic Essence of Laurus nobilis L. on Pro-inflammatoiry Cytokine Gene Expression in Synoviocytes and Macrophage/Monocyte},
journal = {Biomedical Sciences},
volume = {8},
number = {1},
pages = {10-19},
doi = {10.11648/j.bs.20220801.13},
url = {https://doi.org/10.11648/j.bs.20220801.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bs.20220801.13},
abstract = {Osteoarthritis (OA) is a chronic degenerative joint disease with an inflammatory component. It is associated with progressive histological alterations and disabling symptoms. Today, drugs such as glucocorticoids (GCs) and nonsteroidal anti-inflammatory drugs (NSAIDs) are commonly employed for the treatment of osteoarthritis but have serious and life-threatening side effects. The current study aims to evaluate the effects of alcoholic essences of Laurus nobilis L. (AELN) on pro-inflammatory cytokines such as cyclooxygenase-2 (COX-2, isoform), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), interleukin-18 (IL-18), tumor necrosis factor-alpha (TNF-α), and nitric oxide (NO), as well as prostaglandin E2 (PGE2) on inflammatory cells, similar osteoarthritis in synoviocytes, and monocytes/ macrophages, and to compare it with dexamethasone (DEX) and ibuprofen (IBP). After collecting the leaves of the Laurus nobilis L. (LN) and after drying, the essences were collected by the Center for Genetic and Biological Resources of Iran. Synovial cells were isolated from the synovial membrane of the radiocarpal joint cartilage of an 8-month-old Holstein cow. THP-1 cells were prepared from the Pasteur Institute of Iran. Cells were cultivated and exposed to lipopolysaccharide (LPS) stimulation without, or in the presence of, DEX, IBP, or alcoholic essences of Laurus nobilis L. (AELN) The gene expressions of IL-1β, TNF-α, IL-18, COX-2, and iNOS were evaluated by real-time PCR. Concentrations of NO and PGE2 were measured by ELISA methods. Treatment of the studied cell with alcoholic essences of Laurus nobilis L, before stimulation with lipopolysaccharide, reduces the expression of proinflammatory cytokine genes such as cyclooxygenase-2, nitric oxide synthase, interleukin-6, interleukin-1 beta, interleukin-18, tumor necrosis factor-alpha, and It also reduces the production of nitric oxide and prostaglandin E2 by almost 50%. This reduction is significant compared to the 90% reduction due to treatment with dexamethasone and ibuprofen. Significant reduction in the expression of pro-inflammatory cytokines by alcoholic essences of Laurus nobilis L can be considered as a new drug in the treatment of osteoarthritis and requires further studies in laboratory animals and clinical studies.},
year = {2022}
}
TY - JOUR T1 - The Effect of the Alcoholic Essence of Laurus nobilis L. on Pro-inflammatoiry Cytokine Gene Expression in Synoviocytes and Macrophage/Monocyte AU - Hossein Maghsoudi AU - Mahsa Khosrogardi AU - Amir Akbarnejad Eshkalak AU - Younes Tatar Mamaghani AU - Gholamreza Bakhshi Khanaki AU - Enayatollah Yazdanpanah Y1 - 2022/02/16 PY - 2022 N1 - https://doi.org/10.11648/j.bs.20220801.13 DO - 10.11648/j.bs.20220801.13 T2 - Biomedical Sciences JF - Biomedical Sciences JO - Biomedical Sciences SP - 10 EP - 19 PB - Science Publishing Group SN - 2575-3932 UR - https://doi.org/10.11648/j.bs.20220801.13 AB - Osteoarthritis (OA) is a chronic degenerative joint disease with an inflammatory component. It is associated with progressive histological alterations and disabling symptoms. Today, drugs such as glucocorticoids (GCs) and nonsteroidal anti-inflammatory drugs (NSAIDs) are commonly employed for the treatment of osteoarthritis but have serious and life-threatening side effects. The current study aims to evaluate the effects of alcoholic essences of Laurus nobilis L. (AELN) on pro-inflammatory cytokines such as cyclooxygenase-2 (COX-2, isoform), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), interleukin-18 (IL-18), tumor necrosis factor-alpha (TNF-α), and nitric oxide (NO), as well as prostaglandin E2 (PGE2) on inflammatory cells, similar osteoarthritis in synoviocytes, and monocytes/ macrophages, and to compare it with dexamethasone (DEX) and ibuprofen (IBP). After collecting the leaves of the Laurus nobilis L. (LN) and after drying, the essences were collected by the Center for Genetic and Biological Resources of Iran. Synovial cells were isolated from the synovial membrane of the radiocarpal joint cartilage of an 8-month-old Holstein cow. THP-1 cells were prepared from the Pasteur Institute of Iran. Cells were cultivated and exposed to lipopolysaccharide (LPS) stimulation without, or in the presence of, DEX, IBP, or alcoholic essences of Laurus nobilis L. (AELN) The gene expressions of IL-1β, TNF-α, IL-18, COX-2, and iNOS were evaluated by real-time PCR. Concentrations of NO and PGE2 were measured by ELISA methods. Treatment of the studied cell with alcoholic essences of Laurus nobilis L, before stimulation with lipopolysaccharide, reduces the expression of proinflammatory cytokine genes such as cyclooxygenase-2, nitric oxide synthase, interleukin-6, interleukin-1 beta, interleukin-18, tumor necrosis factor-alpha, and It also reduces the production of nitric oxide and prostaglandin E2 by almost 50%. This reduction is significant compared to the 90% reduction due to treatment with dexamethasone and ibuprofen. Significant reduction in the expression of pro-inflammatory cytokines by alcoholic essences of Laurus nobilis L can be considered as a new drug in the treatment of osteoarthritis and requires further studies in laboratory animals and clinical studies. VL - 8 IS - 1 ER -
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