Identification of Class I Integrons Gene in Staphylococcus Strains Isolated from Clinical Samples

Parasto Veise; Rashid Ramazanzadeh; Zahra Dailami Khiababi; Bahare Derakhshi; Nour Amirmozafari

doi:doi:10.11648/j.cb.20130103.11

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Identification of Class I Integrons Gene in Staphylococcus Strains Isolated from Clinical Samples

Parasto Veise, Rashid Ramazanzadeh, Zahra Dailami Khiababi, Bahare Derakhshi, Nour Amirmozafari

Published in Cell Biology (Volume 1, Issue 3)

Received: 12 November 2013 Accepted: Published: 30 December 2013

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Abstract

Introduction and Objectives: Antimicrobial resistance is a major contemporary public health threat. Strategies to contain antimicrobial resistance have been comprehensively set forth, however in developing countries where the need for effective antimicrobials is greatest implementation has proved problematic. Staphylococcus is an important Nasocomial infectious agent which is notorious for rapidly gaining antimicrobial resistance genes. Integrons are a series of mobile genetic elements that are able to express gene cassettes encoding various antibiotic resistances. This study aimed to identify integron class I gene cassettes in clinical Staphylococcus isolates recovered from patients in Sanandaj, Iran hospitals. Materials and Methods: A total of 200 Staphylococci spp. was recovered from nose and throat swabs of patients (ICU and infection wards) in Toohid and Beasat hospitals in Sanandaj, Iran. Following bacterial DNA extraction, Class I Integron gene was detected by PCR. Results: Out of the 200 Staphylococci spp. , 81 (40.5%) isolates were carriers of class I integron . The integron expressing isolates included 35 cases (23.5%) of Staphylococcus epidermidis, 37 cases (40.1%) of Staphylococcus aureus, and 9 cases (36%) of Staphylococcus saprophyticus. Conclusion: Results indicated that frequency of class I integron gene is quite high among clinical Staphylococcus isolates in Sanandaj area. For control of antibiotic resistance spread, screening of clinical samples for these genes and elucidation of their genetic diversity is crucial.

Published in	Cell Biology (Volume 1, Issue 3)
DOI	10.11648/j.cb.20130103.11
Page(s)	24-27
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Staphylococcus Aureus, Coagulase Negative Staphylococcus, Integrons Class I Gene

References

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[14]	Xu, Z., et al., First confirmation of integrons-bearing methicillin-resistant Staphylococcus aureus. Curr Microbiol, 2008, 57(3):p. 264-268.
[15]	Liu H, Wang H, Huang M, Mei Y, Gu B, Wu R, Huang Y, Chen Y, Xu Y, Wang T. Analysis of antimicrobial resistance and class 1 integrons among strains from upper respiratory tract of healthy adults. J Thorac Dis, 2013;5(2):149-55
[16]	Pal, N., et al., Detection of inducible clindamycin resistance among Staphylococcal isolates from different clinical specimens in western India Department of Microbiology, Jaipur, India, 2010, 56(3), 182-185.
[17]	John Burpo , F., A critical review of PCR primer design algorithms and cross hybridization case study. Biochemistry, 2001;218: 1 – 1
[18]	Pal, N., et al., Detection of inducible clindamycin resistance among Staphylococcal isolates from different clinical specimens in western India Department of Microbiology, Jaipur, India, 2010, 56(3), 182-185.
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APA Style

Parasto Veise, Rashid Ramazanzadeh, Zahra Dailami Khiababi, Bahare Derakhshi, Nour Amirmozafari. (2013). Identification of Class I Integrons Gene in Staphylococcus Strains Isolated from Clinical Samples. Cell Biology, 1(3), 24-27. https://doi.org/10.11648/j.cb.20130103.11

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ACS Style

Parasto Veise; Rashid Ramazanzadeh; Zahra Dailami Khiababi; Bahare Derakhshi; Nour Amirmozafari. Identification of Class I Integrons Gene in Staphylococcus Strains Isolated from Clinical Samples. Cell Biol. 2013, 1(3), 24-27. doi: 10.11648/j.cb.20130103.11

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AMA Style

Parasto Veise, Rashid Ramazanzadeh, Zahra Dailami Khiababi, Bahare Derakhshi, Nour Amirmozafari. Identification of Class I Integrons Gene in Staphylococcus Strains Isolated from Clinical Samples. Cell Biol. 2013;1(3):24-27. doi: 10.11648/j.cb.20130103.11

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@article{10.11648/j.cb.20130103.11,
  author = {Parasto Veise and Rashid Ramazanzadeh and Zahra Dailami Khiababi and Bahare Derakhshi and Nour Amirmozafari},
  title = {Identification of Class I Integrons Gene in Staphylococcus Strains Isolated from Clinical Samples},
  journal = {Cell Biology},
  volume = {1},
  number = {3},
  pages = {24-27},
  doi = {10.11648/j.cb.20130103.11},
  url = {https://doi.org/10.11648/j.cb.20130103.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cb.20130103.11},
  abstract = {Introduction and Objectives: Antimicrobial resistance is a major contemporary public health threat. Strategies to contain antimicrobial resistance have been comprehensively set forth, however in developing countries where the need for effective antimicrobials is greatest implementation has proved problematic. Staphylococcus is an important Nasocomial infectious agent which is notorious for rapidly gaining antimicrobial resistance genes. Integrons are a series of mobile genetic elements that are able to express gene cassettes encoding various antibiotic resistances. This study aimed to identify integron class I gene cassettes in clinical Staphylococcus isolates recovered from patients in Sanandaj, Iran hospitals. Materials and Methods: A total of 200 Staphylococci spp. was recovered from nose and throat swabs of patients (ICU and infection wards) in Toohid and Beasat hospitals in Sanandaj, Iran. Following bacterial DNA extraction, Class I Integron gene was detected by PCR. Results: Out of the 200 Staphylococci spp. , 81 (40.5%) isolates were carriers of class I integron . The integron expressing isolates included 35 cases (23.5%) of Staphylococcus epidermidis, 37 cases (40.1%) of Staphylococcus aureus, and 9 cases (36%) of Staphylococcus saprophyticus. Conclusion: Results indicated that frequency of class I integron gene is quite high among clinical Staphylococcus isolates in Sanandaj area. For control of antibiotic resistance spread, screening of clinical samples for these genes and elucidation of their genetic diversity is crucial.},
 year = {2013}
}

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TY  - JOUR
T1  - Identification of Class I Integrons Gene in Staphylococcus Strains Isolated from Clinical Samples
AU  - Parasto Veise
AU  - Rashid Ramazanzadeh
AU  - Zahra Dailami Khiababi
AU  - Bahare Derakhshi
AU  - Nour Amirmozafari
Y1  - 2013/12/30
PY  - 2013
N1  - https://doi.org/10.11648/j.cb.20130103.11
DO  - 10.11648/j.cb.20130103.11
T2  - Cell Biology
JF  - Cell Biology
JO  - Cell Biology
SP  - 24
EP  - 27
PB  - Science Publishing Group
SN  - 2330-0183
UR  - https://doi.org/10.11648/j.cb.20130103.11
AB  - Introduction and Objectives: Antimicrobial resistance is a major contemporary public health threat. Strategies to contain antimicrobial resistance have been comprehensively set forth, however in developing countries where the need for effective antimicrobials is greatest implementation has proved problematic. Staphylococcus is an important Nasocomial infectious agent which is notorious for rapidly gaining antimicrobial resistance genes. Integrons are a series of mobile genetic elements that are able to express gene cassettes encoding various antibiotic resistances. This study aimed to identify integron class I gene cassettes in clinical Staphylococcus isolates recovered from patients in Sanandaj, Iran hospitals. Materials and Methods: A total of 200 Staphylococci spp. was recovered from nose and throat swabs of patients (ICU and infection wards) in Toohid and Beasat hospitals in Sanandaj, Iran. Following bacterial DNA extraction, Class I Integron gene was detected by PCR. Results: Out of the 200 Staphylococci spp. , 81 (40.5%) isolates were carriers of class I integron . The integron expressing isolates included 35 cases (23.5%) of Staphylococcus epidermidis, 37 cases (40.1%) of Staphylococcus aureus, and 9 cases (36%) of Staphylococcus saprophyticus. Conclusion: Results indicated that frequency of class I integron gene is quite high among clinical Staphylococcus isolates in Sanandaj area. For control of antibiotic resistance spread, screening of clinical samples for these genes and elucidation of their genetic diversity is crucial.
VL  - 1
IS  - 3
ER  -

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Author Information

Parasto Veise

Microbiology department, Islamic Azad University of Zanjan
Rashid Ramazanzadeh

Cellular & Molecular Research Center and Microbiology Department, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj- Iran
Zahra Dailami Khiababi

Microbiology department, Islamic Azad University of Zanjan
Bahare Derakhshi

Student Research Committee, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj- Iran
Nour Amirmozafari

Microbiology department, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

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APA Style

Parasto Veise, Rashid Ramazanzadeh, Zahra Dailami Khiababi, Bahare Derakhshi, Nour Amirmozafari. (2013). Identification of Class I Integrons Gene in Staphylococcus Strains Isolated from Clinical Samples. Cell Biology, 1(3), 24-27. https://doi.org/10.11648/j.cb.20130103.11

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ACS Style

Parasto Veise; Rashid Ramazanzadeh; Zahra Dailami Khiababi; Bahare Derakhshi; Nour Amirmozafari. Identification of Class I Integrons Gene in Staphylococcus Strains Isolated from Clinical Samples. Cell Biol. 2013, 1(3), 24-27. doi: 10.11648/j.cb.20130103.11

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AMA Style

Parasto Veise, Rashid Ramazanzadeh, Zahra Dailami Khiababi, Bahare Derakhshi, Nour Amirmozafari. Identification of Class I Integrons Gene in Staphylococcus Strains Isolated from Clinical Samples. Cell Biol. 2013;1(3):24-27. doi: 10.11648/j.cb.20130103.11

Copy | Download

@article{10.11648/j.cb.20130103.11,
  author = {Parasto Veise and Rashid Ramazanzadeh and Zahra Dailami Khiababi and Bahare Derakhshi and Nour Amirmozafari},
  title = {Identification of Class I Integrons Gene in Staphylococcus Strains Isolated from Clinical Samples},
  journal = {Cell Biology},
  volume = {1},
  number = {3},
  pages = {24-27},
  doi = {10.11648/j.cb.20130103.11},
  url = {https://doi.org/10.11648/j.cb.20130103.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cb.20130103.11},
  abstract = {Introduction and Objectives: Antimicrobial resistance is a major contemporary public health threat. Strategies to contain antimicrobial resistance have been comprehensively set forth, however in developing countries where the need for effective antimicrobials is greatest implementation has proved problematic. Staphylococcus is an important Nasocomial infectious agent which is notorious for rapidly gaining antimicrobial resistance genes. Integrons are a series of mobile genetic elements that are able to express gene cassettes encoding various antibiotic resistances. This study aimed to identify integron class I gene cassettes in clinical Staphylococcus isolates recovered from patients in Sanandaj, Iran hospitals. Materials and Methods: A total of 200 Staphylococci spp. was recovered from nose and throat swabs of patients (ICU and infection wards) in Toohid and Beasat hospitals in Sanandaj, Iran. Following bacterial DNA extraction, Class I Integron gene was detected by PCR. Results: Out of the 200 Staphylococci spp. , 81 (40.5%) isolates were carriers of class I integron . The integron expressing isolates included 35 cases (23.5%) of Staphylococcus epidermidis, 37 cases (40.1%) of Staphylococcus aureus, and 9 cases (36%) of Staphylococcus saprophyticus. Conclusion: Results indicated that frequency of class I integron gene is quite high among clinical Staphylococcus isolates in Sanandaj area. For control of antibiotic resistance spread, screening of clinical samples for these genes and elucidation of their genetic diversity is crucial.},
 year = {2013}
}

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TY  - JOUR
T1  - Identification of Class I Integrons Gene in Staphylococcus Strains Isolated from Clinical Samples
AU  - Parasto Veise
AU  - Rashid Ramazanzadeh
AU  - Zahra Dailami Khiababi
AU  - Bahare Derakhshi
AU  - Nour Amirmozafari
Y1  - 2013/12/30
PY  - 2013
N1  - https://doi.org/10.11648/j.cb.20130103.11
DO  - 10.11648/j.cb.20130103.11
T2  - Cell Biology
JF  - Cell Biology
JO  - Cell Biology
SP  - 24
EP  - 27
PB  - Science Publishing Group
SN  - 2330-0183
UR  - https://doi.org/10.11648/j.cb.20130103.11
AB  - Introduction and Objectives: Antimicrobial resistance is a major contemporary public health threat. Strategies to contain antimicrobial resistance have been comprehensively set forth, however in developing countries where the need for effective antimicrobials is greatest implementation has proved problematic. Staphylococcus is an important Nasocomial infectious agent which is notorious for rapidly gaining antimicrobial resistance genes. Integrons are a series of mobile genetic elements that are able to express gene cassettes encoding various antibiotic resistances. This study aimed to identify integron class I gene cassettes in clinical Staphylococcus isolates recovered from patients in Sanandaj, Iran hospitals. Materials and Methods: A total of 200 Staphylococci spp. was recovered from nose and throat swabs of patients (ICU and infection wards) in Toohid and Beasat hospitals in Sanandaj, Iran. Following bacterial DNA extraction, Class I Integron gene was detected by PCR. Results: Out of the 200 Staphylococci spp. , 81 (40.5%) isolates were carriers of class I integron . The integron expressing isolates included 35 cases (23.5%) of Staphylococcus epidermidis, 37 cases (40.1%) of Staphylococcus aureus, and 9 cases (36%) of Staphylococcus saprophyticus. Conclusion: Results indicated that frequency of class I integron gene is quite high among clinical Staphylococcus isolates in Sanandaj area. For control of antibiotic resistance spread, screening of clinical samples for these genes and elucidation of their genetic diversity is crucial.
VL  - 1
IS  - 3
ER  -

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