Production, Partial Purification, and Characterization of Lipase from <i>Aspergillus niger</i> and Its Application in Treatment of Vegetable Oil Effluent

Chimaobi Sunday Vincent; Charles Ogugua Nwuche; Mida Habila Mayel; Sabinus Oscar Onyebuchi Eze

doi:doi:10.11648/j.cbe.20220701.11

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Production, Partial Purification, and Characterization of Lipase from Aspergillus niger and Its Application in Treatment of Vegetable Oil Effluent

Chimaobi Sunday Vincent, Charles Ogugua Nwuche, Mida Habila Mayel, Sabinus Oscar Onyebuchi Eze

Published in Chemical and Biomolecular Engineering (Volume 7, Issue 1)

Received: 15 November 2021 Accepted: 7 December 2021 Published: 29 March 2022

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Abstract

Vegetable oil factories generate considerable quantity of waste. The wastewater from the production process causes severe environmental pollution. Although, several conventional and biological treatment methods have been tested, the outcomes are usually fraught with significant draw backs. The enzymatic method was employed in the present study due to their many beneficial attributes. Lipase was produced by submerged fermentation using the effluent as a sole source of carbon. Upon partial purification, a 3-fold increase in lipase activity was obtained. The enzyme reacted optimally at 50°C, pH of 6.0 and substrate concentration of 7mg/ml. The crude ezyme displayed identical characteristics with the partially purified one. The crude and partially purified lipase were used to treat the wastwater separately. From the results, crude lipase liberated higher fatty acids (1.0591%) from the vegetable oil effluent than the partially purified lipase (0.6066%) did. Kinetic parameters of the enzyme indicate a K_m value of 8 mg/ml and V_max value of 344.83 μmol/min. The results of this study could be applied for the purpose of formulating appropriate policy directives that will aid clean-up of pollution from vegetable oil factories. In addition, vegetable wastewaters can be used as substrates for the production of lipases which could be processed for industiral and biotechnological applications.

Published in	Chemical and Biomolecular Engineering (Volume 7, Issue 1)
DOI	10.11648/j.cbe.20220701.11
Page(s)	1-7
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2022. Published by Science Publishing Group

Keywords

Lipase, Vegetable Oil Effluent, Aspergillus niger, Submerged Fermentation

References

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APA Style

Chimaobi Sunday Vincent, Charles Ogugua Nwuche, Mida Habila Mayel, Sabinus Oscar Onyebuchi Eze. (2022). Production, Partial Purification, and Characterization of Lipase from Aspergillus niger and Its Application in Treatment of Vegetable Oil Effluent. Chemical and Biomolecular Engineering, 7(1), 1-7. https://doi.org/10.11648/j.cbe.20220701.11

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ACS Style

Chimaobi Sunday Vincent; Charles Ogugua Nwuche; Mida Habila Mayel; Sabinus Oscar Onyebuchi Eze. Production, Partial Purification, and Characterization of Lipase from Aspergillus niger and Its Application in Treatment of Vegetable Oil Effluent. Chem. Biomol. Eng. 2022, 7(1), 1-7. doi: 10.11648/j.cbe.20220701.11

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Chimaobi Sunday Vincent, Charles Ogugua Nwuche, Mida Habila Mayel, Sabinus Oscar Onyebuchi Eze. Production, Partial Purification, and Characterization of Lipase from Aspergillus niger and Its Application in Treatment of Vegetable Oil Effluent. Chem Biomol Eng. 2022;7(1):1-7. doi: 10.11648/j.cbe.20220701.11

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@article{10.11648/j.cbe.20220701.11,
  author = {Chimaobi Sunday Vincent and Charles Ogugua Nwuche and Mida Habila Mayel and Sabinus Oscar Onyebuchi Eze},
  title = {Production, Partial Purification, and Characterization of Lipase from Aspergillus niger and Its Application in Treatment of Vegetable Oil Effluent},
  journal = {Chemical and Biomolecular Engineering},
  volume = {7},
  number = {1},
  pages = {1-7},
  doi = {10.11648/j.cbe.20220701.11},
  url = {https://doi.org/10.11648/j.cbe.20220701.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cbe.20220701.11},
  abstract = {Vegetable oil factories generate considerable quantity of waste. The wastewater from the production process causes severe environmental pollution. Although, several conventional and biological treatment methods have been tested, the outcomes are usually fraught with significant draw backs. The enzymatic method was employed in the present study due to their many beneficial attributes. Lipase was produced by submerged fermentation using the effluent as a sole source of carbon. Upon partial purification, a 3-fold increase in lipase activity was obtained. The enzyme reacted optimally at 50°C, pH of 6.0 and substrate concentration of 7mg/ml. The crude ezyme displayed identical characteristics with the partially purified one. The crude and partially purified lipase were used to treat the wastwater separately. From the results, crude lipase liberated higher fatty acids (1.0591%) from the vegetable oil effluent than the partially purified lipase (0.6066%) did. Kinetic parameters of the enzyme indicate a Km value of 8 mg/ml and Vmax value of 344.83 μmol/min. The results of this study could be applied for the purpose of formulating appropriate policy directives that will aid clean-up of pollution from vegetable oil factories. In addition, vegetable wastewaters can be used as substrates for the production of lipases which could be processed for industiral and biotechnological applications.},
 year = {2022}
}

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TY - JOUR
T1 - Production, Partial Purification, and Characterization of Lipase from Aspergillus niger and Its Application in Treatment of Vegetable Oil Effluent
AU - Chimaobi Sunday Vincent
AU - Charles Ogugua Nwuche
AU - Mida Habila Mayel
AU - Sabinus Oscar Onyebuchi Eze
Y1 - 2022/03/29
PY - 2022
N1 - https://doi.org/10.11648/j.cbe.20220701.11
DO - 10.11648/j.cbe.20220701.11
T2 - Chemical and Biomolecular Engineering
JF - Chemical and Biomolecular Engineering
JO - Chemical and Biomolecular Engineering
SP - 1
EP - 7
PB - Science Publishing Group
SN - 2578-8884
UR - https://doi.org/10.11648/j.cbe.20220701.11
AB - Vegetable oil factories generate considerable quantity of waste. The wastewater from the production process causes severe environmental pollution. Although, several conventional and biological treatment methods have been tested, the outcomes are usually fraught with significant draw backs. The enzymatic method was employed in the present study due to their many beneficial attributes. Lipase was produced by submerged fermentation using the effluent as a sole source of carbon. Upon partial purification, a 3-fold increase in lipase activity was obtained. The enzyme reacted optimally at 50°C, pH of 6.0 and substrate concentration of 7mg/ml. The crude ezyme displayed identical characteristics with the partially purified one. The crude and partially purified lipase were used to treat the wastwater separately. From the results, crude lipase liberated higher fatty acids (1.0591%) from the vegetable oil effluent than the partially purified lipase (0.6066%) did. Kinetic parameters of the enzyme indicate a Km value of 8 mg/ml and Vmax value of 344.83 μmol/min. The results of this study could be applied for the purpose of formulating appropriate policy directives that will aid clean-up of pollution from vegetable oil factories. In addition, vegetable wastewaters can be used as substrates for the production of lipases which could be processed for industiral and biotechnological applications.
VL - 7
IS - 1
ER -

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Author Information

Chimaobi Sunday Vincent

Department of Biochemistry, Faculty of Biological Sciences, University of Nigeria, Enugu, Nigeria
Charles Ogugua Nwuche

Department of Microbiology, Faculty of Biological Sciences, University of Nigeria, Enugu, Nigeria
Mida Habila Mayel

Department of Biochemistry, Federal University, Wukari, Nigeria
Sabinus Oscar Onyebuchi Eze

Department of Biochemistry, Faculty of Biological Sciences, University of Nigeria, Enugu, Nigeria

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Chimaobi Sunday Vincent, Charles Ogugua Nwuche, Mida Habila Mayel, Sabinus Oscar Onyebuchi Eze. (2022). Production, Partial Purification, and Characterization of Lipase from Aspergillus niger and Its Application in Treatment of Vegetable Oil Effluent. Chemical and Biomolecular Engineering, 7(1), 1-7. https://doi.org/10.11648/j.cbe.20220701.11

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Chimaobi Sunday Vincent; Charles Ogugua Nwuche; Mida Habila Mayel; Sabinus Oscar Onyebuchi Eze. Production, Partial Purification, and Characterization of Lipase from Aspergillus niger and Its Application in Treatment of Vegetable Oil Effluent. Chem. Biomol. Eng. 2022, 7(1), 1-7. doi: 10.11648/j.cbe.20220701.11

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AMA Style

Chimaobi Sunday Vincent, Charles Ogugua Nwuche, Mida Habila Mayel, Sabinus Oscar Onyebuchi Eze. Production, Partial Purification, and Characterization of Lipase from Aspergillus niger and Its Application in Treatment of Vegetable Oil Effluent. Chem Biomol Eng. 2022;7(1):1-7. doi: 10.11648/j.cbe.20220701.11

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@article{10.11648/j.cbe.20220701.11,
  author = {Chimaobi Sunday Vincent and Charles Ogugua Nwuche and Mida Habila Mayel and Sabinus Oscar Onyebuchi Eze},
  title = {Production, Partial Purification, and Characterization of Lipase from Aspergillus niger and Its Application in Treatment of Vegetable Oil Effluent},
  journal = {Chemical and Biomolecular Engineering},
  volume = {7},
  number = {1},
  pages = {1-7},
  doi = {10.11648/j.cbe.20220701.11},
  url = {https://doi.org/10.11648/j.cbe.20220701.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cbe.20220701.11},
  abstract = {Vegetable oil factories generate considerable quantity of waste. The wastewater from the production process causes severe environmental pollution. Although, several conventional and biological treatment methods have been tested, the outcomes are usually fraught with significant draw backs. The enzymatic method was employed in the present study due to their many beneficial attributes. Lipase was produced by submerged fermentation using the effluent as a sole source of carbon. Upon partial purification, a 3-fold increase in lipase activity was obtained. The enzyme reacted optimally at 50°C, pH of 6.0 and substrate concentration of 7mg/ml. The crude ezyme displayed identical characteristics with the partially purified one. The crude and partially purified lipase were used to treat the wastwater separately. From the results, crude lipase liberated higher fatty acids (1.0591%) from the vegetable oil effluent than the partially purified lipase (0.6066%) did. Kinetic parameters of the enzyme indicate a Km value of 8 mg/ml and Vmax value of 344.83 μmol/min. The results of this study could be applied for the purpose of formulating appropriate policy directives that will aid clean-up of pollution from vegetable oil factories. In addition, vegetable wastewaters can be used as substrates for the production of lipases which could be processed for industiral and biotechnological applications.},
 year = {2022}
}

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