Treatment with Number 2 Feibi Recipe, a Compound Traditional Chinese Medicine Reduces Bleomycin-induced Pulmonary Fibrosis in Mice

Qi Long; Zhao Heng Liu; Jie Niu; Hao Ge Liu; Xiao Feng Gu; Qing Lu Pang; Fang Cao; Yang Jiao

doi:doi:10.11648/j.cmr.20211003.13

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Treatment with Number 2 Feibi Recipe, a Compound Traditional Chinese Medicine Reduces Bleomycin-induced Pulmonary Fibrosis in Mice

Qi Long, Zhao Heng Liu, Jie Niu, Hao Ge Liu, Xiao Feng Gu, Qing Lu Pang, Fang Cao, Yang Jiao

Published in Clinical Medicine Research (Volume 10, Issue 3)

Received: 15 April 2021 Accepted: 8 May 2021 Published: 14 May 2021

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Abstract

Objective: Oxidative stress with reactive oxygen species (ROS) generated from exogenous oxidants and pollutants exposure is involved in the pathogenesis of Idiopathic pulmonary fibrosis (IPF). Number 2 Feibi Recipe (Number 2 FBR) is a traditional Chinese herbal formula which can attenuate the lung injury induced by PM2.5. The present study is to explore the effect and mechanism of Number 2 FBR on bleomycin (BLM)-induced pulmonary fibrosis in C57BL/6Cnc mice. Method: Bleomycin-induced C57BL/6Cnc mice were treated with Number 2 FBR and Sulforphane for two weeks. HE and Masson trichrome staining were performed to evaluate pathological changes in lung tissues. The extent of lung fibrosis was evaluated with fibrosis scores, collagen volume fraction, and hydroxyproline concentration. Levels of SOD and 8-iso-PGF2α in lung tissues were measured by using commercial assay kits. The levels of Nrf2, SOD, GSH-Px, and TGF-β1 relative protein and mRNA in lung tissues were measured by real time PCR and Western blot respectively. Results: The results showed that Number 2 FBR ameliorated bleomycin-induced pathological changes, collagen deposition and significantly decreased fibrosis scores, collagen volume fraction, and hydroxyproline concentration in the mice lungs. Additionally, Number 2 FBR inhibited the expression of 8-iso-prostaglandin F2α (8-iso-PGF2α) and transforming growth factor beta1 (TGF-β1), and increased the expression of Nuclear Factor Erythroid 2-Related Factor 2 (Nrf2), superoxide dismutase (SOD) and Glutathione peroxidase (GSH-Px) in lung tissues. Conclusion: Number 2 FBR has an effect of anti-fibrosis by regulating the lung oxidants and antioxidants balance.

Published in	Clinical Medicine Research (Volume 10, Issue 3)
DOI	10.11648/j.cmr.20211003.13
Page(s)	73-83
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Number 2 Feibi Recipe, Chinese Medicine, Pulmonary Fibrosis, Nrf2, Bleomycin, Mice

References

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Qi Long, Zhao Heng Liu, Jie Niu, Hao Ge Liu, Xiao Feng Gu, et al. (2021). Treatment with Number 2 Feibi Recipe, a Compound Traditional Chinese Medicine Reduces Bleomycin-induced Pulmonary Fibrosis in Mice. Clinical Medicine Research, 10(3), 73-83. https://doi.org/10.11648/j.cmr.20211003.13

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Qi Long; Zhao Heng Liu; Jie Niu; Hao Ge Liu; Xiao Feng Gu, et al. Treatment with Number 2 Feibi Recipe, a Compound Traditional Chinese Medicine Reduces Bleomycin-induced Pulmonary Fibrosis in Mice. Clin. Med. Res. 2021, 10(3), 73-83. doi: 10.11648/j.cmr.20211003.13

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Qi Long, Zhao Heng Liu, Jie Niu, Hao Ge Liu, Xiao Feng Gu, et al. Treatment with Number 2 Feibi Recipe, a Compound Traditional Chinese Medicine Reduces Bleomycin-induced Pulmonary Fibrosis in Mice. Clin Med Res. 2021;10(3):73-83. doi: 10.11648/j.cmr.20211003.13

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@article{10.11648/j.cmr.20211003.13,
  author = {Qi Long and Zhao Heng Liu and Jie Niu and Hao Ge Liu and Xiao Feng Gu and Qing Lu Pang and Fang Cao and Yang Jiao},
  title = {Treatment with Number 2 Feibi Recipe, a Compound Traditional Chinese Medicine Reduces Bleomycin-induced Pulmonary Fibrosis in Mice},
  journal = {Clinical Medicine Research},
  volume = {10},
  number = {3},
  pages = {73-83},
  doi = {10.11648/j.cmr.20211003.13},
  url = {https://doi.org/10.11648/j.cmr.20211003.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cmr.20211003.13},
  abstract = {Objective: Oxidative stress with reactive oxygen species (ROS) generated from exogenous oxidants and pollutants exposure is involved in the pathogenesis of Idiopathic pulmonary fibrosis (IPF). Number 2 Feibi Recipe (Number 2 FBR) is a traditional Chinese herbal formula which can attenuate the lung injury induced by PM2.5. The present study is to explore the effect and mechanism of Number 2 FBR on bleomycin (BLM)-induced pulmonary fibrosis in C57BL/6Cnc mice. Method: Bleomycin-induced C57BL/6Cnc mice were treated with Number 2 FBR and Sulforphane for two weeks. HE and Masson trichrome staining were performed to evaluate pathological changes in lung tissues. The extent of lung fibrosis was evaluated with fibrosis scores, collagen volume fraction, and hydroxyproline concentration. Levels of SOD and 8-iso-PGF2α in lung tissues were measured by using commercial assay kits. The levels of Nrf2, SOD, GSH-Px, and TGF-β1 relative protein and mRNA in lung tissues were measured by real time PCR and Western blot respectively. Results: The results showed that Number 2 FBR ameliorated bleomycin-induced pathological changes, collagen deposition and significantly decreased fibrosis scores, collagen volume fraction, and hydroxyproline concentration in the mice lungs. Additionally, Number 2 FBR inhibited the expression of 8-iso-prostaglandin F2α (8-iso-PGF2α) and transforming growth factor beta1 (TGF-β1), and increased the expression of Nuclear Factor Erythroid 2-Related Factor 2 (Nrf2), superoxide dismutase (SOD) and Glutathione peroxidase (GSH-Px) in lung tissues. Conclusion: Number 2 FBR has an effect of anti-fibrosis by regulating the lung oxidants and antioxidants balance.},
 year = {2021}
}

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TY  - JOUR
T1  - Treatment with Number 2 Feibi Recipe, a Compound Traditional Chinese Medicine Reduces Bleomycin-induced Pulmonary Fibrosis in Mice
AU  - Qi Long
AU  - Zhao Heng Liu
AU  - Jie Niu
AU  - Hao Ge Liu
AU  - Xiao Feng Gu
AU  - Qing Lu Pang
AU  - Fang Cao
AU  - Yang Jiao
Y1  - 2021/05/14
PY  - 2021
N1  - https://doi.org/10.11648/j.cmr.20211003.13
DO  - 10.11648/j.cmr.20211003.13
T2  - Clinical Medicine Research
JF  - Clinical Medicine Research
JO  - Clinical Medicine Research
SP  - 73
EP  - 83
PB  - Science Publishing Group
SN  - 2326-9057
UR  - https://doi.org/10.11648/j.cmr.20211003.13
AB  - Objective: Oxidative stress with reactive oxygen species (ROS) generated from exogenous oxidants and pollutants exposure is involved in the pathogenesis of Idiopathic pulmonary fibrosis (IPF). Number 2 Feibi Recipe (Number 2 FBR) is a traditional Chinese herbal formula which can attenuate the lung injury induced by PM2.5. The present study is to explore the effect and mechanism of Number 2 FBR on bleomycin (BLM)-induced pulmonary fibrosis in C57BL/6Cnc mice. Method: Bleomycin-induced C57BL/6Cnc mice were treated with Number 2 FBR and Sulforphane for two weeks. HE and Masson trichrome staining were performed to evaluate pathological changes in lung tissues. The extent of lung fibrosis was evaluated with fibrosis scores, collagen volume fraction, and hydroxyproline concentration. Levels of SOD and 8-iso-PGF2α in lung tissues were measured by using commercial assay kits. The levels of Nrf2, SOD, GSH-Px, and TGF-β1 relative protein and mRNA in lung tissues were measured by real time PCR and Western blot respectively. Results: The results showed that Number 2 FBR ameliorated bleomycin-induced pathological changes, collagen deposition and significantly decreased fibrosis scores, collagen volume fraction, and hydroxyproline concentration in the mice lungs. Additionally, Number 2 FBR inhibited the expression of 8-iso-prostaglandin F2α (8-iso-PGF2α) and transforming growth factor beta1 (TGF-β1), and increased the expression of Nuclear Factor Erythroid 2-Related Factor 2 (Nrf2), superoxide dismutase (SOD) and Glutathione peroxidase (GSH-Px) in lung tissues. Conclusion: Number 2 FBR has an effect of anti-fibrosis by regulating the lung oxidants and antioxidants balance.
VL  - 10
IS  - 3
ER  -

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Author Information

Qi Long

Graduate School, Beijing University of Chinese Medicine, Beijing, China
Zhao Heng Liu

School of Life Sciences, Beijing University of Chinese Medicine, Beijing, China
Jie Niu

Dongfang Affiliated Hospital, Beijing University of Chinese Medicine, Beijing, China
Hao Ge Liu

Graduate School, Beijing University of Chinese Medicine, Beijing, China
Xiao Feng Gu

Graduate School, Beijing University of Chinese Medicine, Beijing, China
Qing Lu Pang

Graduate School, Beijing University of Chinese Medicine, Beijing, China
Fang Cao

Dongfang Affiliated Hospital, Beijing University of Chinese Medicine, Beijing, China
Yang Jiao

Dongfang Affiliated Hospital, Beijing University of Chinese Medicine, Beijing, China

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Qi Long, Zhao Heng Liu, Jie Niu, Hao Ge Liu, Xiao Feng Gu, et al. (2021). Treatment with Number 2 Feibi Recipe, a Compound Traditional Chinese Medicine Reduces Bleomycin-induced Pulmonary Fibrosis in Mice. Clinical Medicine Research, 10(3), 73-83. https://doi.org/10.11648/j.cmr.20211003.13

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ACS Style

Qi Long; Zhao Heng Liu; Jie Niu; Hao Ge Liu; Xiao Feng Gu, et al. Treatment with Number 2 Feibi Recipe, a Compound Traditional Chinese Medicine Reduces Bleomycin-induced Pulmonary Fibrosis in Mice. Clin. Med. Res. 2021, 10(3), 73-83. doi: 10.11648/j.cmr.20211003.13

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Qi Long, Zhao Heng Liu, Jie Niu, Hao Ge Liu, Xiao Feng Gu, et al. Treatment with Number 2 Feibi Recipe, a Compound Traditional Chinese Medicine Reduces Bleomycin-induced Pulmonary Fibrosis in Mice. Clin Med Res. 2021;10(3):73-83. doi: 10.11648/j.cmr.20211003.13

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@article{10.11648/j.cmr.20211003.13,
  author = {Qi Long and Zhao Heng Liu and Jie Niu and Hao Ge Liu and Xiao Feng Gu and Qing Lu Pang and Fang Cao and Yang Jiao},
  title = {Treatment with Number 2 Feibi Recipe, a Compound Traditional Chinese Medicine Reduces Bleomycin-induced Pulmonary Fibrosis in Mice},
  journal = {Clinical Medicine Research},
  volume = {10},
  number = {3},
  pages = {73-83},
  doi = {10.11648/j.cmr.20211003.13},
  url = {https://doi.org/10.11648/j.cmr.20211003.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cmr.20211003.13},
  abstract = {Objective: Oxidative stress with reactive oxygen species (ROS) generated from exogenous oxidants and pollutants exposure is involved in the pathogenesis of Idiopathic pulmonary fibrosis (IPF). Number 2 Feibi Recipe (Number 2 FBR) is a traditional Chinese herbal formula which can attenuate the lung injury induced by PM2.5. The present study is to explore the effect and mechanism of Number 2 FBR on bleomycin (BLM)-induced pulmonary fibrosis in C57BL/6Cnc mice. Method: Bleomycin-induced C57BL/6Cnc mice were treated with Number 2 FBR and Sulforphane for two weeks. HE and Masson trichrome staining were performed to evaluate pathological changes in lung tissues. The extent of lung fibrosis was evaluated with fibrosis scores, collagen volume fraction, and hydroxyproline concentration. Levels of SOD and 8-iso-PGF2α in lung tissues were measured by using commercial assay kits. The levels of Nrf2, SOD, GSH-Px, and TGF-β1 relative protein and mRNA in lung tissues were measured by real time PCR and Western blot respectively. Results: The results showed that Number 2 FBR ameliorated bleomycin-induced pathological changes, collagen deposition and significantly decreased fibrosis scores, collagen volume fraction, and hydroxyproline concentration in the mice lungs. Additionally, Number 2 FBR inhibited the expression of 8-iso-prostaglandin F2α (8-iso-PGF2α) and transforming growth factor beta1 (TGF-β1), and increased the expression of Nuclear Factor Erythroid 2-Related Factor 2 (Nrf2), superoxide dismutase (SOD) and Glutathione peroxidase (GSH-Px) in lung tissues. Conclusion: Number 2 FBR has an effect of anti-fibrosis by regulating the lung oxidants and antioxidants balance.},
 year = {2021}
}

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TY  - JOUR
T1  - Treatment with Number 2 Feibi Recipe, a Compound Traditional Chinese Medicine Reduces Bleomycin-induced Pulmonary Fibrosis in Mice
AU  - Qi Long
AU  - Zhao Heng Liu
AU  - Jie Niu
AU  - Hao Ge Liu
AU  - Xiao Feng Gu
AU  - Qing Lu Pang
AU  - Fang Cao
AU  - Yang Jiao
Y1  - 2021/05/14
PY  - 2021
N1  - https://doi.org/10.11648/j.cmr.20211003.13
DO  - 10.11648/j.cmr.20211003.13
T2  - Clinical Medicine Research
JF  - Clinical Medicine Research
JO  - Clinical Medicine Research
SP  - 73
EP  - 83
PB  - Science Publishing Group
SN  - 2326-9057
UR  - https://doi.org/10.11648/j.cmr.20211003.13
AB  - Objective: Oxidative stress with reactive oxygen species (ROS) generated from exogenous oxidants and pollutants exposure is involved in the pathogenesis of Idiopathic pulmonary fibrosis (IPF). Number 2 Feibi Recipe (Number 2 FBR) is a traditional Chinese herbal formula which can attenuate the lung injury induced by PM2.5. The present study is to explore the effect and mechanism of Number 2 FBR on bleomycin (BLM)-induced pulmonary fibrosis in C57BL/6Cnc mice. Method: Bleomycin-induced C57BL/6Cnc mice were treated with Number 2 FBR and Sulforphane for two weeks. HE and Masson trichrome staining were performed to evaluate pathological changes in lung tissues. The extent of lung fibrosis was evaluated with fibrosis scores, collagen volume fraction, and hydroxyproline concentration. Levels of SOD and 8-iso-PGF2α in lung tissues were measured by using commercial assay kits. The levels of Nrf2, SOD, GSH-Px, and TGF-β1 relative protein and mRNA in lung tissues were measured by real time PCR and Western blot respectively. Results: The results showed that Number 2 FBR ameliorated bleomycin-induced pathological changes, collagen deposition and significantly decreased fibrosis scores, collagen volume fraction, and hydroxyproline concentration in the mice lungs. Additionally, Number 2 FBR inhibited the expression of 8-iso-prostaglandin F2α (8-iso-PGF2α) and transforming growth factor beta1 (TGF-β1), and increased the expression of Nuclear Factor Erythroid 2-Related Factor 2 (Nrf2), superoxide dismutase (SOD) and Glutathione peroxidase (GSH-Px) in lung tissues. Conclusion: Number 2 FBR has an effect of anti-fibrosis by regulating the lung oxidants and antioxidants balance.
VL  - 10
IS  - 3
ER  -

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