Non-tuberculous mycobacteria (NTM) are microorganisms of the genus Mycobacterium. They are widely present in the environment (soil, water, sediment, aquatic plants ....). They are responsible for many infections as reported by several authors. The purpose of this study is to isolate and identify mycobacteria in the water and sediments found in hyperendemic areas and hypoendemic of Buruli ulcer. A total of 473 samples were obtained. As follows, 251 samples from water and 222 from sediment distributed according to sampling sites. The sample decontamination was performed with Cetylpiridium Chloride (CPC), followed by neutralization with phosphate buffer. A total of 8 species (12.3%) were identified in our study. 50.77% of species identified were found in hyper endemic zones against, 49.23% of species at the hypo-endemic zones. Species like M. peregrinum, like M. smegmatis, like M. peregrinum, M. immunogenicum, M. chelonae, M. mucogenicum, M. abscessus, M. sp. were isolated in this study. The species M, peregrinum (13.84%) was the most common in all sites, except in the sites of Bodo and Bouaké. This study reveals the presence of fast growing mycobacteria such as M. peregrinum in water and in sediment in Côte d`Ivoire, which represents the potential risk of contamination in humans especially in people who are in permanent contact with water.
| Published in | Frontiers in Environmental Microbiology (Volume 2, Issue 6) |
| DOI | 10.11648/j.fem.20160206.11 |
| Page(s) | 28-33 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Mycobacterium, Buruli Ulcer, Non-tuberculous Mycobacteria, Culture
| [1] | Griffith DE, Aksamit T, Brown-Elliott BA, Catanzaro A, Daley C, Gordin F, Holland SM, Horsburgh R, Huitt G, Iademarco MF, Iseman M, Olivier K, Ruoss S, von Reyn CF, Wallace RJ Jr, Winthrop K, An official ATS/IDSA statement: Diagnosis, treatment, and prevention of non tuberculous mycobacterial diseases. Am J Respir Crit Care Med 2007; 175: 367-416. |
| [2] | Winthrop Kevin L, M. D, Abrams Marcy, R. N, Yakrus Mitchell, M. S, M. P. H, Ira Schwartz, R. N, M. P. H, Janet Ely, B. A, Gillies Duncan, B. A, And Vugia Duc J, M. D, M. P. H. (2002). An outbreak of mycobacterial furunculosis associated with footbaths at a nail salon. New England Journal of Medicine 346 (18): 1366-1371. |
| [3] | Sniezek P. J, Graham B. S, Busch HB, Lederman ER, Lim ML, Poggemyer K, Kao A, Mizrahi M, Washabaugh G, Yakrus M., Winthrop K. (2003). Rapidly growing mycobacterial infections after pedicures. Archives of Dermatological Research 139 (1): 629-634. |
| [4] | Marsollier L, Robert R, Aubry J, Saint Andre J P, Kouakou H, Legras P, Manceau A L, Mahaza C, Carbonnelle B, Aquatic insects as a vector for Mycobacterium ulcerans. Appl. Environ. Microbiol. 68,4623-4628 (2002). |
| [5] | Marsollier L., Stinear T, Aubry J, Saint André JP, Robert R, Legras P, Manceau AL, Audrain C, Bourdon S, Kouakou H, Carbonnelle B (2004) Aquatic plants stimulate the growth of and biofilm formation by Mycobacterium ulcerans in axenic culture and harbor these bacteria in the environment. App Env. Microbiol 70: 1097–1103. |
| [6] | Ziza Jean Marc et Desplaces Nicole (2006) Infections ostéoarticulaires à mycobactéries atypiques. Revue du rhumatisme, 73: 394-400. |
| [7] | Euzéby J. P. (2010) Techniques de diagnostiques en mycobactériologie. Dictionnaire de Bactériologie Vétérinaire http:// www.mycobacterie.fr. |
| [8] | Inderlied C. B, Kemper C. A et Bermudez L. M (1993). The Mycobacterium avium complex. Clinical Microbiology Reviews 6 (3): 266-310. |
| [9] | Caruso G, Passàli F. M, Salerni L, Molinaro G. et Messina M. (2009). Head and neck mycobacterial infections in pediatric patients. International Journal of Pediatric Otorhinolaryngology 73 (1): 38-41. |
| [10] | Del Rio Camacho G, Soriano Guillén L, Flandes Aldeyturriaga J, Hernández García B et Bernácer Borja M. (2010). Endobronchial atypical mycobacteria in an immunocompetent child. Pediatric Pulmonology 45 (5): 511-513. |
| [11] | Chilima BZ, Clark IM, Floyd S, Fine PE, Hirsch PR (2006). Distribution of environmental mycobacteria in Karonga District Northern Malawi. Appl. Environ. Microbiol. 72: 2243-2250. |
| [12] | Kubica, G P, Kaufmann A J, and Dye W E 1964. Comments on the use of the new mucolytic agent, N-acetyl-l-cysteine, as a sputum digestant for the isolation of mycobacteria. Am. Rev. Respir. Dis.89:284-286. |
| [13] | Trujillo, M., E. Velazquez, R. M. Kroppenstedt, P. Schumann, R. Rivas, P. F. Mateos, et al. (2004). Mycobacterium psychrotolerans sp. nov., isolated from pond water near a uranium mine. International Journal of Systematic and Evolutionary Microbiology 54 (5): 1459-1463. |
| [14] | Brown, B. A., B. Springer, V. A. Steingrube, R. W. Wilson, G. E. Pfyffer, M. J. Garcia, et al. (1999). Mycobacterium wolinskyi sp. nov. And Mycobacterium goodie sp. nov., two new rapidly growing species related to Mycobacterium smegmatis and associated with human wound infections: a cooperative study from the International Working Group on Mycobacterial Taxonomy. International Journal of Systematic Bacteriology 49 (4): 1493-1511. |
| [15] | Falkinham JO III: Epidemiology of infection by non tuberculous mycobacteria. Clin Microbiol Rev 1996, 9: 177-215. |
| [16] | Dailloux M, Laurain C, Weber R, Hartemann P: Water and non tuberculous mycobacteria. Water Res 2010, 33:2219-2228. |
| [17] | Martín-Casabona N, Bahrmand A R, Bennedsen J, Østergaard Thomsen V, Curcio M, Fauville-Dufaux M, Feldman K, Havelkova M, Katila M-L, Köksalan K, Pereira M F, Rodrigues F, Pfyffer G E, Portaels F, Rosselló Urgell J, Rüsch-Gerdes S, Spanish Group for Non-Tuberculosis Mycobacteria, Tortoli E, Vincent V, Watt B (2004). Non-tuberculous mycobacteria : patterns of isolation. A multi-country retrospective survey. Int. J. Tuberc. Lung Dis. 8: 1186-1193. |
| [18] | Brown et Wallace, 1992, Skin, soft tissue, and bone infections due to Mycobacterium chelonae chelonae: importance of prior corticosteroid therapy, frequency of disseminated infections, and resistance to oral antimicrobials other than clarithromycin, J Infect Dis. 1992 Aug; 166 (2): 405-12. |
| [19] | Schiavone S, Coquery M (2011). Guide d’échantillonnage et de pré- traitement des sédiments en milieu continental pour les analyses physico-chimiques de la DCE. Cemagref, 24 p. |
| [20] | Clovice Kankya, Adrian Muwonge, Berit Djønne, Musso Munyeme, John Opuda-Asibo1, Eystein Skjerve, James Oloya1, Vigdis Edvardsen and Tone B Johansen, 2011, Isolation of non-tuberculous mycobacteria from pastoral ecosystems of Uganda: Public Health significance BMC Public Health, 11: 320 1471-2458. |
| [21] | Tim Stinear, Ford T, and Vincent V 2004. Analytical methods for the detection of waterborne and environmental pathogenic mycobacteria, p. 55–73. In S. Pedley, J. Bartram, G. Rees, A. Dufour, and J. Cotruvo (ed.), Pathogenic mycobacteria in water: a guide to public health consequences, monitoring and management. IWA Publishing, London, United Kingdom. |
| [22] | Carbonnelle B, Dailloux M., Lea L, Jeanne M., Chantal P, Cahier de formation mycobactéries, mycobacterioses, 2003. |
| [23] | Barksdale L. and Kim K. S. (1977): Mycobacterium. Bacteriol. Rev. 41: 217. |
| [24] | Runyon E H (1959): Anonymous mycobacteria in pulmonary disease. Med. Clin. N. Amer. 43: 273-290. |
| [25] | Mohammad Rahbar, Aboulfathlamei, Homayoon Babazadeh and Shoherh Afsharyavari, 2010, Isolation of rapid growing mycobacteria from soil and water in Iran, African journal of Biotechnology, vol 9(24), pp. 3618-3621. |
| [26] | Kirschner RA Jr, Parker BC, Falkinham JO III: Epidemiology of infection by non tuberculous mycobacteria. Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium scrofulaceum in acid, brown-water swamps of the south eastern United States and their association with environmental variables. Am Rev Respir Dis 1992, 145: 271-275. |
| [27] | Stinear TP, Seemann T, Pidot S, Frigui W, Reysset G, et al. Reduction evolution and niche adaptation inferred from the genome of Mycobacterium ulcerans, the causative agent of Buruli ulcer. Genome Res. 2007; 17: 192-200. |
| [28] | Kamala T, Paramasivan C N, Herbert D, Venkatesan P, and Prabhakar R. 1994. Evaluation of procedure for isolation of non tuberculous mycobacteria from soil and water. Appl. Environ. Microbiol. 60: 1021–1024. |
| [29] | Hunter P, J. Lee, G. Nichol, M. Rutter, S. Surman, L. Weldon, D. Biegon, T. Fazakerley, F. Drobnewski, and P. Morrell 2001 fate of mycobacterium avium complex in drinking water and distribution system. Mallow, Buckinghamshire: foundation for water research; 2001. Reporty no; dw10815. |
| [30] | R. M. Atlas and R. Bartha (1997). Microbial ecology: fundamentals and applications. Benjamin. Cummings Science, New York. |
| [31] | RW. Pickup, G. Rhodes Arnott S., Sidi-Boomedine K., Bull TJ., Weightman A., Hurley M., et Hermon-Taylor J. (2005). Mycobacterium avium subsp. paratuberculosis in the catchment area and water of the river Taff in South Wales, United Kingdom, and its potential relationship to clustering of Crohn’s disease cases in the city of Cardiff. Appl. Environ. Microbiol. 71: 2130-2139. |
| [32] | Parashar D., Chaulan DS., Sharma VD., Chauhan A., Chauhan SVS., et Katoch VM. (2004). Optimisation of procedures for isolation of mycobacteria from soil and water samples obtained in northern India. Appl. Environ. Microbiol. 70: 3751-3753. |
| [33] | P. R. J. Gangadharam, et P. A. Jenkins (1998a). Mycobacteria, basic aspects Eds. New York, in International Thomson Publishing. 1: 400. |
| [34] | Prescott, L. M., J. P. Harley, D. A. Klein, C. M. Bacq-Calberg et J. Dusart (2003). Les bactéries : Les Gram-positifs riches en G-C. Microbiologie. J. Prescott, J. Harley and D. Klein Eds. Bruxelles, in De Boeck Université. 1: 541. |
| [35] | Shinnick, T. M. et R. C. Good (1994). Mycobacterial taxonomy. European Journal of Clinical Microbiology and Infectious Diseases 13 (11): 884- 901. |
| [36] | Tsukamura, M 1984 Identification of mycobacteria. Mycobacteriosis research laboratory, National Chubu Hospital, Obu, Aichi, Japan. |
| [37] | Yates MD, Pozniack A, Uttley AHC, et al. isolation of environmental mycobacteria from clinical specimen in South East England, 1973-1993, Int J Tuberc Lung Dis 1997; 1: 75-80. |
| [38] | Zamarioli LA, Coelho AG, Pereira CM, Nascimento AC, Ueki SY, Chimara E: Descriptive study of the frequency of non tuberculous mycobacteria in the Baixada Santista region of the state of Sao Paulo, Brazil. J Bras Pneumol 2008, 34:590-594.42. |
| [39] | Buijtels PC, van der Sande MA, de Graaff CS, Parkinson S, Verbrugh HA, Petit PL: Non tuberculous mycobacteria, Zambia. Emerg Infect Dis 2009, 15:242-249. |
| [40] | Buijtels Patricia C. A. M., Pieter L. C. Petit, Henri A. Verbrugh, Alex van Belkum, and Dick van Soolingen. Isolation of Non tuberculous Mycobacteria in Zambia: Eight Case Reports; Journal of clinical microbiology, Dec. 2005, p. 6020–6026 Vol. 43, No. 12. |
| [41] | Williamson HR, Benbow ME, Nguyen KD, Beach board DC, Kimbirauskas RK, et al. (2008) Distribution of Mycobacterium ulcerans in Buruli Ulcer Endemic and Non-Endemic Aquatic Sites in Ghana. PLoS Negl Trop Dis 2 (3): e205. doi:10.1371/journal. pntd.0000205. |
APA Style
Vakou N. Sabine, Coulibaly Kalpy J., Aka N’guett, Kakou Ngazoa E. Solange, Coulibaly N’golo D., et al. (2016). Phenotypic Profile of Isolated Strains of Environmental Mycobacteria in the Buruli Ulcer Endemic Zones in Cote d`Ivoire (2015). Frontiers in Environmental Microbiology, 2(6), 28-33. https://doi.org/10.11648/j.fem.20160206.11
ACS Style
Vakou N. Sabine; Coulibaly Kalpy J.; Aka N’guett; Kakou Ngazoa E. Solange; Coulibaly N’golo D., et al. Phenotypic Profile of Isolated Strains of Environmental Mycobacteria in the Buruli Ulcer Endemic Zones in Cote d`Ivoire (2015). Front. Environ. Microbiol. 2016, 2(6), 28-33. doi: 10.11648/j.fem.20160206.11
AMA Style
Vakou N. Sabine, Coulibaly Kalpy J., Aka N’guett, Kakou Ngazoa E. Solange, Coulibaly N’golo D., et al. Phenotypic Profile of Isolated Strains of Environmental Mycobacteria in the Buruli Ulcer Endemic Zones in Cote d`Ivoire (2015). Front Environ Microbiol. 2016;2(6):28-33. doi: 10.11648/j.fem.20160206.11
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Download@article{10.11648/j.fem.20160206.11,
author = {Vakou N. Sabine and Coulibaly Kalpy J. and Aka N’guett and Kakou Ngazoa E. Solange and Coulibaly N’golo D. and Djaman Allico and Dosso Mireille},
title = {Phenotypic Profile of Isolated Strains of Environmental Mycobacteria in the Buruli Ulcer Endemic Zones in Cote d`Ivoire (2015)},
journal = {Frontiers in Environmental Microbiology},
volume = {2},
number = {6},
pages = {28-33},
doi = {10.11648/j.fem.20160206.11},
url = {https://doi.org/10.11648/j.fem.20160206.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20160206.11},
abstract = {Non-tuberculous mycobacteria (NTM) are microorganisms of the genus Mycobacterium. They are widely present in the environment (soil, water, sediment, aquatic plants ....). They are responsible for many infections as reported by several authors. The purpose of this study is to isolate and identify mycobacteria in the water and sediments found in hyperendemic areas and hypoendemic of Buruli ulcer. A total of 473 samples were obtained. As follows, 251 samples from water and 222 from sediment distributed according to sampling sites. The sample decontamination was performed with Cetylpiridium Chloride (CPC), followed by neutralization with phosphate buffer. A total of 8 species (12.3%) were identified in our study. 50.77% of species identified were found in hyper endemic zones against, 49.23% of species at the hypo-endemic zones. Species like M. peregrinum, like M. smegmatis, like M. peregrinum, M. immunogenicum, M. chelonae, M. mucogenicum, M. abscessus, M. sp. were isolated in this study. The species M, peregrinum (13.84%) was the most common in all sites, except in the sites of Bodo and Bouaké. This study reveals the presence of fast growing mycobacteria such as M. peregrinum in water and in sediment in Côte d`Ivoire, which represents the potential risk of contamination in humans especially in people who are in permanent contact with water.},
year = {2016}
}
TY - JOUR T1 - Phenotypic Profile of Isolated Strains of Environmental Mycobacteria in the Buruli Ulcer Endemic Zones in Cote d`Ivoire (2015) AU - Vakou N. Sabine AU - Coulibaly Kalpy J. AU - Aka N’guett AU - Kakou Ngazoa E. Solange AU - Coulibaly N’golo D. AU - Djaman Allico AU - Dosso Mireille Y1 - 2016/11/30 PY - 2016 N1 - https://doi.org/10.11648/j.fem.20160206.11 DO - 10.11648/j.fem.20160206.11 T2 - Frontiers in Environmental Microbiology JF - Frontiers in Environmental Microbiology JO - Frontiers in Environmental Microbiology SP - 28 EP - 33 PB - Science Publishing Group SN - 2469-8067 UR - https://doi.org/10.11648/j.fem.20160206.11 AB - Non-tuberculous mycobacteria (NTM) are microorganisms of the genus Mycobacterium. They are widely present in the environment (soil, water, sediment, aquatic plants ....). They are responsible for many infections as reported by several authors. The purpose of this study is to isolate and identify mycobacteria in the water and sediments found in hyperendemic areas and hypoendemic of Buruli ulcer. A total of 473 samples were obtained. As follows, 251 samples from water and 222 from sediment distributed according to sampling sites. The sample decontamination was performed with Cetylpiridium Chloride (CPC), followed by neutralization with phosphate buffer. A total of 8 species (12.3%) were identified in our study. 50.77% of species identified were found in hyper endemic zones against, 49.23% of species at the hypo-endemic zones. Species like M. peregrinum, like M. smegmatis, like M. peregrinum, M. immunogenicum, M. chelonae, M. mucogenicum, M. abscessus, M. sp. were isolated in this study. The species M, peregrinum (13.84%) was the most common in all sites, except in the sites of Bodo and Bouaké. This study reveals the presence of fast growing mycobacteria such as M. peregrinum in water and in sediment in Côte d`Ivoire, which represents the potential risk of contamination in humans especially in people who are in permanent contact with water. VL - 2 IS - 6 ER -
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