Isolation, Screening and Biochemical Identification of Bacteria with Purifying Potential in the Effluents of a Paint Manufacturing Industry

Emmanuel Joël Ndzobo Ndzana; Marie Frederic Tavea; Josiane Bella; Romeo Tagnikeu Fobasso; Wilfred Atangana; Joel Styve Tcheugoue; Angelin Yanick Kuissie; Felecite Njen; Regis Tanga Tanga; Youagang Gougueu Harris Stephane; Emmanuel Mpondo Mpondo

doi:doi:10.11648/j.fem.20220801.12

Isolation, Screening and Biochemical Identification of Bacteria with Purifying Potential in the Effluents of a Paint Manufacturing Industry

Emmanuel Joël Ndzobo Ndzana, Marie Frederic Tavea, Josiane Bella, Romeo Tagnikeu Fobasso, Wilfred Atangana, Joel Styve Tcheugoue, Angelin Yanick Kuissie, Felecite Njen, Regis Tanga Tanga, Youagang Gougueu Harris Stephane, Emmanuel Mpondo Mpondo

Published in Frontiers in Environmental Microbiology (Volume 8, Issue 1)

Received: 9 April 2022 Accepted: 29 April 2022 Published: 10 May 2022

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Abstract

The technology based on the use of micro-organisms, in this case bacteria isolated from sites contaminated by industries, represents an alternative option for the treatment of wastewater before discharge into the environment. To this end, we carried out research in the city of Douala on the isolation, selection and identification of indigenous bacteria, potential for the bio-purification of effluents from a paint manufacturing industry located in the Logbaba industrial zone (Douala Cameroon). The bacteria were isolated from soils contaminated by effluents from the said industry. A programme of three selection tests in mineral saline liquid medium was implemented for the choice of isolates. The selection criterion was based on the growth of the isolates as assessed by OD measurement with a spectrophotometer (BIOBASE) at 600 nm. At the end of the selection process, 05 isolates (IS10, IS20, IS25, IS35 and IS41) were considered to be the best potential candidates for effluent bio-purification. On the basis of a pre-test identification completed by the API20E system, they were identified as Stenotrophomonas maltophilia (IS41); Pseudomonas luteola (IS20 and IS35); Pseudomonas fluorescens/putida (IS25) and Yersinia pseudotuberculosis (IS10) Exploiting the bio-purification capacities of these bacteria in the context of biological treatment of these polluting aqueous discharges could contribute to the preservation of the environment and human health. In the course of our further work, the bio-purification potential of these isolates will be evaluated.

Published in	Frontiers in Environmental Microbiology (Volume 8, Issue 1)
DOI	10.11648/j.fem.20220801.12
Page(s)	6-12
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Isolation, Isolate, Identification, Effluent, Scrubber, Paint

References

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APA Style

Emmanuel Joël Ndzobo Ndzana, Marie Frederic Tavea, Josiane Bella, Romeo Tagnikeu Fobasso, Wilfred Atangana, et al. (2022). Isolation, Screening and Biochemical Identification of Bacteria with Purifying Potential in the Effluents of a Paint Manufacturing Industry. Frontiers in Environmental Microbiology, 8(1), 6-12. https://doi.org/10.11648/j.fem.20220801.12

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ACS Style

Emmanuel Joël Ndzobo Ndzana; Marie Frederic Tavea; Josiane Bella; Romeo Tagnikeu Fobasso; Wilfred Atangana, et al. Isolation, Screening and Biochemical Identification of Bacteria with Purifying Potential in the Effluents of a Paint Manufacturing Industry. Front. Environ. Microbiol. 2022, 8(1), 6-12. doi: 10.11648/j.fem.20220801.12

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AMA Style

Emmanuel Joël Ndzobo Ndzana, Marie Frederic Tavea, Josiane Bella, Romeo Tagnikeu Fobasso, Wilfred Atangana, et al. Isolation, Screening and Biochemical Identification of Bacteria with Purifying Potential in the Effluents of a Paint Manufacturing Industry. Front Environ Microbiol. 2022;8(1):6-12. doi: 10.11648/j.fem.20220801.12

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@article{10.11648/j.fem.20220801.12,
  author = {Emmanuel Joël Ndzobo Ndzana and Marie Frederic Tavea and Josiane Bella and Romeo Tagnikeu Fobasso and Wilfred Atangana and Joel Styve Tcheugoue and Angelin Yanick Kuissie and Felecite Njen and Regis Tanga Tanga and Youagang Gougueu Harris Stephane and Emmanuel Mpondo Mpondo},
  title = {Isolation, Screening and Biochemical Identification of Bacteria with Purifying Potential in the Effluents of a Paint Manufacturing Industry},
  journal = {Frontiers in Environmental Microbiology},
  volume = {8},
  number = {1},
  pages = {6-12},
  doi = {10.11648/j.fem.20220801.12},
  url = {https://doi.org/10.11648/j.fem.20220801.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20220801.12},
  abstract = {The technology based on the use of micro-organisms, in this case bacteria isolated from sites contaminated by industries, represents an alternative option for the treatment of wastewater before discharge into the environment. To this end, we carried out research in the city of Douala on the isolation, selection and identification of indigenous bacteria, potential for the bio-purification of effluents from a paint manufacturing industry located in the Logbaba industrial zone (Douala Cameroon). The bacteria were isolated from soils contaminated by effluents from the said industry. A programme of three selection tests in mineral saline liquid medium was implemented for the choice of isolates. The selection criterion was based on the growth of the isolates as assessed by OD measurement with a spectrophotometer (BIOBASE) at 600 nm. At the end of the selection process, 05 isolates (IS10, IS20, IS25, IS35 and IS41) were considered to be the best potential candidates for effluent bio-purification. On the basis of a pre-test identification completed by the API20E system, they were identified as Stenotrophomonas maltophilia (IS41); Pseudomonas luteola (IS20 and IS35); Pseudomonas fluorescens/putida (IS25) and Yersinia pseudotuberculosis (IS10) Exploiting the bio-purification capacities of these bacteria in the context of biological treatment of these polluting aqueous discharges could contribute to the preservation of the environment and human health. In the course of our further work, the bio-purification potential of these isolates will be evaluated.},
 year = {2022}
}

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TY  - JOUR
T1  - Isolation, Screening and Biochemical Identification of Bacteria with Purifying Potential in the Effluents of a Paint Manufacturing Industry
AU  - Emmanuel Joël Ndzobo Ndzana
AU  - Marie Frederic Tavea
AU  - Josiane Bella
AU  - Romeo Tagnikeu Fobasso
AU  - Wilfred Atangana
AU  - Joel Styve Tcheugoue
AU  - Angelin Yanick Kuissie
AU  - Felecite Njen
AU  - Regis Tanga Tanga
AU  - Youagang Gougueu Harris Stephane
AU  - Emmanuel Mpondo Mpondo
Y1  - 2022/05/10
PY  - 2022
N1  - https://doi.org/10.11648/j.fem.20220801.12
DO  - 10.11648/j.fem.20220801.12
T2  - Frontiers in Environmental Microbiology
JF  - Frontiers in Environmental Microbiology
JO  - Frontiers in Environmental Microbiology
SP  - 6
EP  - 12
PB  - Science Publishing Group
SN  - 2469-8067
UR  - https://doi.org/10.11648/j.fem.20220801.12
AB  - The technology based on the use of micro-organisms, in this case bacteria isolated from sites contaminated by industries, represents an alternative option for the treatment of wastewater before discharge into the environment. To this end, we carried out research in the city of Douala on the isolation, selection and identification of indigenous bacteria, potential for the bio-purification of effluents from a paint manufacturing industry located in the Logbaba industrial zone (Douala Cameroon). The bacteria were isolated from soils contaminated by effluents from the said industry. A programme of three selection tests in mineral saline liquid medium was implemented for the choice of isolates. The selection criterion was based on the growth of the isolates as assessed by OD measurement with a spectrophotometer (BIOBASE) at 600 nm. At the end of the selection process, 05 isolates (IS10, IS20, IS25, IS35 and IS41) were considered to be the best potential candidates for effluent bio-purification. On the basis of a pre-test identification completed by the API20E system, they were identified as Stenotrophomonas maltophilia (IS41); Pseudomonas luteola (IS20 and IS35); Pseudomonas fluorescens/putida (IS25) and Yersinia pseudotuberculosis (IS10) Exploiting the bio-purification capacities of these bacteria in the context of biological treatment of these polluting aqueous discharges could contribute to the preservation of the environment and human health. In the course of our further work, the bio-purification potential of these isolates will be evaluated.
VL  - 8
IS  - 1
ER  -

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Author Information

Emmanuel Joël Ndzobo Ndzana
Marie Frederic Tavea

Biochemistry Laboratory, Faculty of Science, University of Douala, Douala, Cameroon
Josiane Bella

Biochemistry Laboratory, Faculty of Science, University of Douala, Douala, Cameroon
Romeo Tagnikeu Fobasso

Biochemistry Laboratory, Faculty of Science, University of Douala, Douala, Cameroon
Wilfred Atangana

Biochemistry Laboratory, Faculty of Science, University of Douala, Douala, Cameroon
Joel Styve Tcheugoue

Biochemistry Laboratory, Faculty of Science, University of Douala, Douala, Cameroon
Angelin Yanick Kuissie

Biochemistry Laboratory, Faculty of Science, University of Douala, Douala, Cameroon
Felecite Njen

Biochemistry Laboratory, Faculty of Science, University of Douala, Douala, Cameroon
Regis Tanga Tanga

Biochemistry Laboratory, Faculty of Science, University of Douala, Douala, Cameroon
Youagang Gougueu Harris Stephane

Plant Biology Laboratory, Faculty of Science, University of Douala, Douala, Cameroon
Emmanuel Mpondo Mpondo

Department of Pharmaceutical Sciences, Faculty of Medicine and Pharmaceutical Sciences, University of Douala, Douala, Cameroon

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APA Style

Emmanuel Joël Ndzobo Ndzana, Marie Frederic Tavea, Josiane Bella, Romeo Tagnikeu Fobasso, Wilfred Atangana, et al. (2022). Isolation, Screening and Biochemical Identification of Bacteria with Purifying Potential in the Effluents of a Paint Manufacturing Industry. Frontiers in Environmental Microbiology, 8(1), 6-12. https://doi.org/10.11648/j.fem.20220801.12

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ACS Style

Emmanuel Joël Ndzobo Ndzana; Marie Frederic Tavea; Josiane Bella; Romeo Tagnikeu Fobasso; Wilfred Atangana, et al. Isolation, Screening and Biochemical Identification of Bacteria with Purifying Potential in the Effluents of a Paint Manufacturing Industry. Front. Environ. Microbiol. 2022, 8(1), 6-12. doi: 10.11648/j.fem.20220801.12

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AMA Style

Emmanuel Joël Ndzobo Ndzana, Marie Frederic Tavea, Josiane Bella, Romeo Tagnikeu Fobasso, Wilfred Atangana, et al. Isolation, Screening and Biochemical Identification of Bacteria with Purifying Potential in the Effluents of a Paint Manufacturing Industry. Front Environ Microbiol. 2022;8(1):6-12. doi: 10.11648/j.fem.20220801.12

Copy | Download

@article{10.11648/j.fem.20220801.12,
  author = {Emmanuel Joël Ndzobo Ndzana and Marie Frederic Tavea and Josiane Bella and Romeo Tagnikeu Fobasso and Wilfred Atangana and Joel Styve Tcheugoue and Angelin Yanick Kuissie and Felecite Njen and Regis Tanga Tanga and Youagang Gougueu Harris Stephane and Emmanuel Mpondo Mpondo},
  title = {Isolation, Screening and Biochemical Identification of Bacteria with Purifying Potential in the Effluents of a Paint Manufacturing Industry},
  journal = {Frontiers in Environmental Microbiology},
  volume = {8},
  number = {1},
  pages = {6-12},
  doi = {10.11648/j.fem.20220801.12},
  url = {https://doi.org/10.11648/j.fem.20220801.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20220801.12},
  abstract = {The technology based on the use of micro-organisms, in this case bacteria isolated from sites contaminated by industries, represents an alternative option for the treatment of wastewater before discharge into the environment. To this end, we carried out research in the city of Douala on the isolation, selection and identification of indigenous bacteria, potential for the bio-purification of effluents from a paint manufacturing industry located in the Logbaba industrial zone (Douala Cameroon). The bacteria were isolated from soils contaminated by effluents from the said industry. A programme of three selection tests in mineral saline liquid medium was implemented for the choice of isolates. The selection criterion was based on the growth of the isolates as assessed by OD measurement with a spectrophotometer (BIOBASE) at 600 nm. At the end of the selection process, 05 isolates (IS10, IS20, IS25, IS35 and IS41) were considered to be the best potential candidates for effluent bio-purification. On the basis of a pre-test identification completed by the API20E system, they were identified as Stenotrophomonas maltophilia (IS41); Pseudomonas luteola (IS20 and IS35); Pseudomonas fluorescens/putida (IS25) and Yersinia pseudotuberculosis (IS10) Exploiting the bio-purification capacities of these bacteria in the context of biological treatment of these polluting aqueous discharges could contribute to the preservation of the environment and human health. In the course of our further work, the bio-purification potential of these isolates will be evaluated.},
 year = {2022}
}

Copy | Download

TY  - JOUR
T1  - Isolation, Screening and Biochemical Identification of Bacteria with Purifying Potential in the Effluents of a Paint Manufacturing Industry
AU  - Emmanuel Joël Ndzobo Ndzana
AU  - Marie Frederic Tavea
AU  - Josiane Bella
AU  - Romeo Tagnikeu Fobasso
AU  - Wilfred Atangana
AU  - Joel Styve Tcheugoue
AU  - Angelin Yanick Kuissie
AU  - Felecite Njen
AU  - Regis Tanga Tanga
AU  - Youagang Gougueu Harris Stephane
AU  - Emmanuel Mpondo Mpondo
Y1  - 2022/05/10
PY  - 2022
N1  - https://doi.org/10.11648/j.fem.20220801.12
DO  - 10.11648/j.fem.20220801.12
T2  - Frontiers in Environmental Microbiology
JF  - Frontiers in Environmental Microbiology
JO  - Frontiers in Environmental Microbiology
SP  - 6
EP  - 12
PB  - Science Publishing Group
SN  - 2469-8067
UR  - https://doi.org/10.11648/j.fem.20220801.12
AB  - The technology based on the use of micro-organisms, in this case bacteria isolated from sites contaminated by industries, represents an alternative option for the treatment of wastewater before discharge into the environment. To this end, we carried out research in the city of Douala on the isolation, selection and identification of indigenous bacteria, potential for the bio-purification of effluents from a paint manufacturing industry located in the Logbaba industrial zone (Douala Cameroon). The bacteria were isolated from soils contaminated by effluents from the said industry. A programme of three selection tests in mineral saline liquid medium was implemented for the choice of isolates. The selection criterion was based on the growth of the isolates as assessed by OD measurement with a spectrophotometer (BIOBASE) at 600 nm. At the end of the selection process, 05 isolates (IS10, IS20, IS25, IS35 and IS41) were considered to be the best potential candidates for effluent bio-purification. On the basis of a pre-test identification completed by the API20E system, they were identified as Stenotrophomonas maltophilia (IS41); Pseudomonas luteola (IS20 and IS35); Pseudomonas fluorescens/putida (IS25) and Yersinia pseudotuberculosis (IS10) Exploiting the bio-purification capacities of these bacteria in the context of biological treatment of these polluting aqueous discharges could contribute to the preservation of the environment and human health. In the course of our further work, the bio-purification potential of these isolates will be evaluated.
VL  - 8
IS  - 1
ER  -

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