The production of indole acetic acid (IAA) by plant growth-promoting microorganisms is one of the most important factors for plant growth. Present research work deals with the characterization and optimization of different physiological conditions for IAA production by the fungus Talaromyces trachyspermus. Different environmental factors and medium components were optimized for the production of IAA by fungal culture. Effect of pH, temperature, aeration and concentration of precursor i.e. L-Tryptophan was evaluated on the biomass of fungus along with the IAA production. This work also focused on the effect of different carbon and nitrogen sources in the media for the growth of Talaromyces trachyspermus and IAA production The maximum production of IAA was observed in 15 days of incubation under the condition of 6.5 pH, 28°C temperature, and 140 RPM, in presence of 0.5% of tryptophan, 4% glucose and 0.1% sodium nitrate. After optimization, the yield of IAA by fungal culture was increased up to 0.78 fold as compared to initial production. IAA production by fungal culture was confirmed by thin-layer chromatography and HPLC analysis. Optimization of IAA production by Talaromyces trachyspermus can be useful for the large-scale production of agriculturally important bioactive metabolites. Moreover, the plant growth-promoting efficiency of this fungal culture makes it novel bio-inoculants for sustainable agriculture.
| Published in | Frontiers in Environmental Microbiology (Volume 8, Issue 2) |
| DOI | 10.11648/j.fem.20220802.12 |
| Page(s) | 25-34 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Indole Acetic Acid, Plant Growth-Promoting Fungi, Talaromyces trachyspermus, Optimization, Sustainable Agriculture
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APA Style
Smriti Chouhan, Lalit Kumar Chaurasia, Anil Prakash. (2022). Optimization and Characterization of Indole Acetic Acid Producing Efficiency of Talaromyces trachyspermus for Sustainable Agro-practices. Frontiers in Environmental Microbiology, 8(2), 25-34. https://doi.org/10.11648/j.fem.20220802.12
ACS Style
Smriti Chouhan; Lalit Kumar Chaurasia; Anil Prakash. Optimization and Characterization of Indole Acetic Acid Producing Efficiency of Talaromyces trachyspermus for Sustainable Agro-practices. Front. Environ. Microbiol. 2022, 8(2), 25-34. doi: 10.11648/j.fem.20220802.12
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Download@article{10.11648/j.fem.20220802.12,
author = {Smriti Chouhan and Lalit Kumar Chaurasia and Anil Prakash},
title = {Optimization and Characterization of Indole Acetic Acid Producing Efficiency of Talaromyces trachyspermus for Sustainable Agro-practices},
journal = {Frontiers in Environmental Microbiology},
volume = {8},
number = {2},
pages = {25-34},
doi = {10.11648/j.fem.20220802.12},
url = {https://doi.org/10.11648/j.fem.20220802.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20220802.12},
abstract = {The production of indole acetic acid (IAA) by plant growth-promoting microorganisms is one of the most important factors for plant growth. Present research work deals with the characterization and optimization of different physiological conditions for IAA production by the fungus Talaromyces trachyspermus. Different environmental factors and medium components were optimized for the production of IAA by fungal culture. Effect of pH, temperature, aeration and concentration of precursor i.e. L-Tryptophan was evaluated on the biomass of fungus along with the IAA production. This work also focused on the effect of different carbon and nitrogen sources in the media for the growth of Talaromyces trachyspermus and IAA production The maximum production of IAA was observed in 15 days of incubation under the condition of 6.5 pH, 28°C temperature, and 140 RPM, in presence of 0.5% of tryptophan, 4% glucose and 0.1% sodium nitrate. After optimization, the yield of IAA by fungal culture was increased up to 0.78 fold as compared to initial production. IAA production by fungal culture was confirmed by thin-layer chromatography and HPLC analysis. Optimization of IAA production by Talaromyces trachyspermus can be useful for the large-scale production of agriculturally important bioactive metabolites. Moreover, the plant growth-promoting efficiency of this fungal culture makes it novel bio-inoculants for sustainable agriculture.},
year = {2022}
}
TY - JOUR T1 - Optimization and Characterization of Indole Acetic Acid Producing Efficiency of Talaromyces trachyspermus for Sustainable Agro-practices AU - Smriti Chouhan AU - Lalit Kumar Chaurasia AU - Anil Prakash Y1 - 2022/05/24 PY - 2022 N1 - https://doi.org/10.11648/j.fem.20220802.12 DO - 10.11648/j.fem.20220802.12 T2 - Frontiers in Environmental Microbiology JF - Frontiers in Environmental Microbiology JO - Frontiers in Environmental Microbiology SP - 25 EP - 34 PB - Science Publishing Group SN - 2469-8067 UR - https://doi.org/10.11648/j.fem.20220802.12 AB - The production of indole acetic acid (IAA) by plant growth-promoting microorganisms is one of the most important factors for plant growth. Present research work deals with the characterization and optimization of different physiological conditions for IAA production by the fungus Talaromyces trachyspermus. Different environmental factors and medium components were optimized for the production of IAA by fungal culture. Effect of pH, temperature, aeration and concentration of precursor i.e. L-Tryptophan was evaluated on the biomass of fungus along with the IAA production. This work also focused on the effect of different carbon and nitrogen sources in the media for the growth of Talaromyces trachyspermus and IAA production The maximum production of IAA was observed in 15 days of incubation under the condition of 6.5 pH, 28°C temperature, and 140 RPM, in presence of 0.5% of tryptophan, 4% glucose and 0.1% sodium nitrate. After optimization, the yield of IAA by fungal culture was increased up to 0.78 fold as compared to initial production. IAA production by fungal culture was confirmed by thin-layer chromatography and HPLC analysis. Optimization of IAA production by Talaromyces trachyspermus can be useful for the large-scale production of agriculturally important bioactive metabolites. Moreover, the plant growth-promoting efficiency of this fungal culture makes it novel bio-inoculants for sustainable agriculture. VL - 8 IS - 2 ER -
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