Freshwater Linked Resistance Profile and Prevalence of Escherichia Coli Producing ESBL Type CTX-M Strains in Cameroon Urban Cities

Cecile Ingrid Djuikoue; Farid Wega; Audrey Limou Dayomo; Cedric Dylan Seugnou Nana; Dorine Ngatcheu Ekeu; Celianthe Guegang Guegang; Dana Djouela Djoulako; Kensese Sontin Mossanda; Venant Tchokonte Nana; Omer Njajou

doi:doi:10.11648/j.fem.20220803.12

Freshwater Linked Resistance Profile and Prevalence of Escherichia Coli Producing ESBL Type CTX-M Strains in Cameroon Urban Cities

Cecile Ingrid Djuikoue, Farid Wega, Audrey Limou Dayomo, Cedric Dylan Seugnou Nana, Dorine Ngatcheu Ekeu, Celianthe Guegang Guegang, Dana Djouela Djoulako, Kensese Sontin Mossanda, Venant Tchokonte Nana, Omer Njajou

Published in Frontiers in Environmental Microbiology (Volume 8, Issue 3)

Received: 9 August 2022 Accepted: 26 August 2022 Published: 5 September 2022

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Abstract

Mutualistic human digestive bacteria resistant to antibiotics released into the environment have negative effects on health. The aim of this study was to describe the presence of Escherichia coli (E. coli) strains producing extended spectrum β-lactamases of the CTX-M type strains in three urban cities of Cameroon. A total of 79 E. coli strains resistant to cefotaxime were isolated from 200 freshwater samples collected and inoculated in EMB medium supplemented with cefotaxime. Isolated colonies were subjected to an antibiogram according to CA-SFM 2019 and the risk factors associated with their prevalence were also assessed. The results showed that 51% of samples were positive for E. coli CTX-M, a large portion of which (77.4%) produced an extended spectrum β-lactamases-CTX-M type. Meanwhile, only 59% of strains were resistant to β-lactamases. A co-resistance to cotrimoxazole (81%), aminoglycosides (57.3%), quinolones (43%), and fluoroquinolones (17.7%) has been reported. Interestingly, E. coli resistant to cefotaxime strains was influenced by the points of collection, latrines proximity and human activities. A multivariate analysis indicated 39.5% of E. coli ESBL CTX-M strains prevalence associated with proximity to latrines and human activities. The Increasing E. coli ESBL CTX-M strains prevalence in Cameroon is indeed a public health concern. Therefore, a “One Health Approach Operationalization Program” that would include sensitizing the population and improving sanitation interventions in effective waste management, is crucial in maintaining a healthy environment.

Published in	Frontiers in Environmental Microbiology (Volume 8, Issue 3)
DOI	10.11648/j.fem.20220803.12
Page(s)	55-61
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Surface Water, Escherichia Coli, Esbl Type Ctx-M, Antibiotics, Risk Factors

References

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Cecile Ingrid Djuikoue, Farid Wega, Audrey Limou Dayomo, Cedric Dylan Seugnou Nana, Dorine Ngatcheu Ekeu, et al. (2022). Freshwater Linked Resistance Profile and Prevalence of Escherichia Coli Producing ESBL Type CTX-M Strains in Cameroon Urban Cities. Frontiers in Environmental Microbiology, 8(3), 55-61. https://doi.org/10.11648/j.fem.20220803.12

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Cecile Ingrid Djuikoue; Farid Wega; Audrey Limou Dayomo; Cedric Dylan Seugnou Nana; Dorine Ngatcheu Ekeu, et al. Freshwater Linked Resistance Profile and Prevalence of Escherichia Coli Producing ESBL Type CTX-M Strains in Cameroon Urban Cities. Front. Environ. Microbiol. 2022, 8(3), 55-61. doi: 10.11648/j.fem.20220803.12

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Cecile Ingrid Djuikoue, Farid Wega, Audrey Limou Dayomo, Cedric Dylan Seugnou Nana, Dorine Ngatcheu Ekeu, et al. Freshwater Linked Resistance Profile and Prevalence of Escherichia Coli Producing ESBL Type CTX-M Strains in Cameroon Urban Cities. Front Environ Microbiol. 2022;8(3):55-61. doi: 10.11648/j.fem.20220803.12

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@article{10.11648/j.fem.20220803.12,
  author = {Cecile Ingrid Djuikoue and Farid Wega and Audrey Limou Dayomo and Cedric Dylan Seugnou Nana and Dorine Ngatcheu Ekeu and Celianthe Guegang Guegang and Dana Djouela Djoulako and Kensese Sontin Mossanda and Venant Tchokonte Nana and Omer Njajou},
  title = {Freshwater Linked Resistance Profile and Prevalence of Escherichia Coli Producing ESBL Type CTX-M Strains in Cameroon Urban Cities},
  journal = {Frontiers in Environmental Microbiology},
  volume = {8},
  number = {3},
  pages = {55-61},
  doi = {10.11648/j.fem.20220803.12},
  url = {https://doi.org/10.11648/j.fem.20220803.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20220803.12},
  abstract = {Mutualistic human digestive bacteria resistant to antibiotics released into the environment have negative effects on health. The aim of this study was to describe the presence of Escherichia coli (E. coli) strains producing extended spectrum β-lactamases of the CTX-M type strains in three urban cities of Cameroon. A total of 79 E. coli strains resistant to cefotaxime were isolated from 200 freshwater samples collected and inoculated in EMB medium supplemented with cefotaxime. Isolated colonies were subjected to an antibiogram according to CA-SFM 2019 and the risk factors associated with their prevalence were also assessed. The results showed that 51% of samples were positive for E. coli CTX-M, a large portion of which (77.4%) produced an extended spectrum β-lactamases-CTX-M type. Meanwhile, only 59% of strains were resistant to β-lactamases. A co-resistance to cotrimoxazole (81%), aminoglycosides (57.3%), quinolones (43%), and fluoroquinolones (17.7%) has been reported. Interestingly, E. coli resistant to cefotaxime strains was influenced by the points of collection, latrines proximity and human activities. A multivariate analysis indicated 39.5% of E. coli ESBL CTX-M strains prevalence associated with proximity to latrines and human activities. The Increasing E. coli ESBL CTX-M strains prevalence in Cameroon is indeed a public health concern. Therefore, a “One Health Approach Operationalization Program” that would include sensitizing the population and improving sanitation interventions in effective waste management, is crucial in maintaining a healthy environment.},
 year = {2022}
}

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TY - JOUR
T1 - Freshwater Linked Resistance Profile and Prevalence of Escherichia Coli Producing ESBL Type CTX-M Strains in Cameroon Urban Cities
AU - Cecile Ingrid Djuikoue
AU - Farid Wega
AU - Audrey Limou Dayomo
AU - Cedric Dylan Seugnou Nana
AU - Dorine Ngatcheu Ekeu
AU - Celianthe Guegang Guegang
AU - Dana Djouela Djoulako
AU - Kensese Sontin Mossanda
AU - Venant Tchokonte Nana
AU - Omer Njajou
Y1 - 2022/09/05
PY - 2022
N1 - https://doi.org/10.11648/j.fem.20220803.12
DO - 10.11648/j.fem.20220803.12
T2 - Frontiers in Environmental Microbiology
JF - Frontiers in Environmental Microbiology
JO - Frontiers in Environmental Microbiology
SP - 55
EP - 61
PB - Science Publishing Group
SN - 2469-8067
UR - https://doi.org/10.11648/j.fem.20220803.12
AB - Mutualistic human digestive bacteria resistant to antibiotics released into the environment have negative effects on health. The aim of this study was to describe the presence of Escherichia coli (E. coli) strains producing extended spectrum β-lactamases of the CTX-M type strains in three urban cities of Cameroon. A total of 79 E. coli strains resistant to cefotaxime were isolated from 200 freshwater samples collected and inoculated in EMB medium supplemented with cefotaxime. Isolated colonies were subjected to an antibiogram according to CA-SFM 2019 and the risk factors associated with their prevalence were also assessed. The results showed that 51% of samples were positive for E. coli CTX-M, a large portion of which (77.4%) produced an extended spectrum β-lactamases-CTX-M type. Meanwhile, only 59% of strains were resistant to β-lactamases. A co-resistance to cotrimoxazole (81%), aminoglycosides (57.3%), quinolones (43%), and fluoroquinolones (17.7%) has been reported. Interestingly, E. coli resistant to cefotaxime strains was influenced by the points of collection, latrines proximity and human activities. A multivariate analysis indicated 39.5% of E. coli ESBL CTX-M strains prevalence associated with proximity to latrines and human activities. The Increasing E. coli ESBL CTX-M strains prevalence in Cameroon is indeed a public health concern. Therefore, a “One Health Approach Operationalization Program” that would include sensitizing the population and improving sanitation interventions in effective waste management, is crucial in maintaining a healthy environment.
VL - 8
IS - 3
ER -

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Author Information

Cecile Ingrid Djuikoue

Faculty of Health Sciences, University des Montagnes, Bangangte, Cameroon
Farid Wega

Faculty of Health Sciences, University des Montagnes, Bangangte, Cameroon
Audrey Limou Dayomo

Faculty of Health Sciences, University des Montagnes, Bangangte, Cameroon
Cedric Dylan Seugnou Nana

Faculty of Health Sciences, University des Montagnes, Bangangte, Cameroon
Dorine Ngatcheu Ekeu

Faculty of Health Sciences, University des Montagnes, Bangangte, Cameroon
Celianthe Guegang Guegang

Faculty of Sciences, University of Douala, Douala, Cameroon
Dana Djouela Djoulako

Faculty of Health Sciences, University des Montagnes, Bangangte, Cameroon
Kensese Sontin Mossanda

Faculty of Health Sciences, University des Montagnes, Bangangte, Cameroon
Venant Tchokonte Nana

Faculty of Health Sciences, University des Montagnes, Bangangte, Cameroon
Omer Njajou

Foundation Prevention and Control, Nde Division Prefecture, Bangangte, Cameroon

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Cecile Ingrid Djuikoue, Farid Wega, Audrey Limou Dayomo, Cedric Dylan Seugnou Nana, Dorine Ngatcheu Ekeu, et al. (2022). Freshwater Linked Resistance Profile and Prevalence of Escherichia Coli Producing ESBL Type CTX-M Strains in Cameroon Urban Cities. Frontiers in Environmental Microbiology, 8(3), 55-61. https://doi.org/10.11648/j.fem.20220803.12

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ACS Style

Cecile Ingrid Djuikoue; Farid Wega; Audrey Limou Dayomo; Cedric Dylan Seugnou Nana; Dorine Ngatcheu Ekeu, et al. Freshwater Linked Resistance Profile and Prevalence of Escherichia Coli Producing ESBL Type CTX-M Strains in Cameroon Urban Cities. Front. Environ. Microbiol. 2022, 8(3), 55-61. doi: 10.11648/j.fem.20220803.12

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AMA Style

Cecile Ingrid Djuikoue, Farid Wega, Audrey Limou Dayomo, Cedric Dylan Seugnou Nana, Dorine Ngatcheu Ekeu, et al. Freshwater Linked Resistance Profile and Prevalence of Escherichia Coli Producing ESBL Type CTX-M Strains in Cameroon Urban Cities. Front Environ Microbiol. 2022;8(3):55-61. doi: 10.11648/j.fem.20220803.12

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@article{10.11648/j.fem.20220803.12,
  author = {Cecile Ingrid Djuikoue and Farid Wega and Audrey Limou Dayomo and Cedric Dylan Seugnou Nana and Dorine Ngatcheu Ekeu and Celianthe Guegang Guegang and Dana Djouela Djoulako and Kensese Sontin Mossanda and Venant Tchokonte Nana and Omer Njajou},
  title = {Freshwater Linked Resistance Profile and Prevalence of Escherichia Coli Producing ESBL Type CTX-M Strains in Cameroon Urban Cities},
  journal = {Frontiers in Environmental Microbiology},
  volume = {8},
  number = {3},
  pages = {55-61},
  doi = {10.11648/j.fem.20220803.12},
  url = {https://doi.org/10.11648/j.fem.20220803.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20220803.12},
  abstract = {Mutualistic human digestive bacteria resistant to antibiotics released into the environment have negative effects on health. The aim of this study was to describe the presence of Escherichia coli (E. coli) strains producing extended spectrum β-lactamases of the CTX-M type strains in three urban cities of Cameroon. A total of 79 E. coli strains resistant to cefotaxime were isolated from 200 freshwater samples collected and inoculated in EMB medium supplemented with cefotaxime. Isolated colonies were subjected to an antibiogram according to CA-SFM 2019 and the risk factors associated with their prevalence were also assessed. The results showed that 51% of samples were positive for E. coli CTX-M, a large portion of which (77.4%) produced an extended spectrum β-lactamases-CTX-M type. Meanwhile, only 59% of strains were resistant to β-lactamases. A co-resistance to cotrimoxazole (81%), aminoglycosides (57.3%), quinolones (43%), and fluoroquinolones (17.7%) has been reported. Interestingly, E. coli resistant to cefotaxime strains was influenced by the points of collection, latrines proximity and human activities. A multivariate analysis indicated 39.5% of E. coli ESBL CTX-M strains prevalence associated with proximity to latrines and human activities. The Increasing E. coli ESBL CTX-M strains prevalence in Cameroon is indeed a public health concern. Therefore, a “One Health Approach Operationalization Program” that would include sensitizing the population and improving sanitation interventions in effective waste management, is crucial in maintaining a healthy environment.},
 year = {2022}
}

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