Multi Traits of Phosphate Solublizing Bacterial and Fungal Isolates and Evaluation of Their Potential as Biofertilizer Agent for Coffee Production

Reshid Abafita Abawari; Fasil Asefa Tuji; Diriba Muleta Yadete

doi:doi:10.11648/j.ijaas.20210701.11

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Multi Traits of Phosphate Solublizing Bacterial and Fungal Isolates and Evaluation of Their Potential as Biofertilizer Agent for Coffee Production

Reshid Abafita Abawari, Fasil Asefa Tuji, Diriba Muleta Yadete

Published in International Journal of Applied Agricultural Sciences (Volume 7, Issue 1)

Received: 7 December 2020 Accepted: 17 December 2020 Published: 12 January 2021

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Abstract

Exploitation of phosphate solubilizing bacteria (PSB) and fungi as bioinoculants instead of chemical fertilizers is known to promote plant growth through the supply of plant nutrients. In view of this, the present investigation was planned to assess the phytobeneficial traits of phosphate solublizing bacterial and fungal isolates recovered from coffee arabica rhizosphere/ vermicompost and to determine their potential in growth promotion of coffee seedlings. They were isolated and purified following standard methods. A total number of 154 bacteria and 72 fungi were isolated from vermicompost and coffee rhizosphere. Out of these, twelve potent bacterial and nine fungal isolates were selected and investigated. Among twelve bacterial isolates, three of them showed significant potential to solubilize Ca₃ (PO₄)₂ and had phytobeneficial traits, viz, indole acetic acid, NH₃, HCN production and N-fixing ability. The three bacterial isolates (RCHVCB₁, RScB1.19 and RMaB2.11) exhibited also remarkable tolerance to ecophysiological factors such as heavy metal, acidity and salinity. These potent isolates were selected for further identification based on morphological and biochemical characteristics and presumptively identified as genera of Pseudomonas (RCHVCB₁) and Bacillus (RScB1.19 and RMaB2.11). Similarly, three fungal isolates with superior phosphate solubilizeation ability were characterized and identified as genera Penicillium (RSCF1.19) and Aspergillus (RCHVCF2 and RLVCF2). Consequently, these three efficient bacterial and fungal isolates were evaluated on the coffee seed germination on Petri dish based trial under laboratory condition. The results of inoculated seeds showed significant (p≤0.05) differences in germination rate and vigor index compared to the control. Among all inoculums RScB1.19, RMaB2.11+RSCF1.19 and RMaB2.11 + RLVCF2 showed significantly (p≤0.05) high germination rate (20.59%) over the control (13.33%). Moreover, a single inoculation of RLVCF2, RSCF1.19 and co-inoculation of RMaB2.11 with RLVCF2 also showed significant (p≤0.05) mean root length (1.31cm) and mean shoot length (1.48cm) over the control. These effective bacterial and fungal solublizers can be recommended under field condition as biofertilizer agent and reducing the cost required for chemical fertilizers and providing a step forward towards sustainable agriculture.

Published in	International Journal of Applied Agricultural Sciences (Volume 7, Issue 1)
DOI	10.11648/j.ijaas.20210701.11
Page(s)	1-15
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Germination, Phosphate Solubilization, Bioinoculants, Phytobeneficial, Ecophysiology

References

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Reshid Abafita Abawari, Fasil Asefa Tuji, Diriba Muleta Yadete. (2021). Multi Traits of Phosphate Solublizing Bacterial and Fungal Isolates and Evaluation of Their Potential as Biofertilizer Agent for Coffee Production. International Journal of Applied Agricultural Sciences, 7(1), 1-15. https://doi.org/10.11648/j.ijaas.20210701.11

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Reshid Abafita Abawari; Fasil Asefa Tuji; Diriba Muleta Yadete. Multi Traits of Phosphate Solublizing Bacterial and Fungal Isolates and Evaluation of Their Potential as Biofertilizer Agent for Coffee Production. Int. J. Appl. Agric. Sci. 2021, 7(1), 1-15. doi: 10.11648/j.ijaas.20210701.11

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Reshid Abafita Abawari, Fasil Asefa Tuji, Diriba Muleta Yadete. Multi Traits of Phosphate Solublizing Bacterial and Fungal Isolates and Evaluation of Their Potential as Biofertilizer Agent for Coffee Production. Int J Appl Agric Sci. 2021;7(1):1-15. doi: 10.11648/j.ijaas.20210701.11

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@article{10.11648/j.ijaas.20210701.11,
  author = {Reshid Abafita Abawari and Fasil Asefa Tuji and Diriba Muleta Yadete},
  title = {Multi Traits of Phosphate Solublizing Bacterial and Fungal Isolates and Evaluation of Their Potential as Biofertilizer Agent for Coffee Production},
  journal = {International Journal of Applied Agricultural Sciences},
  volume = {7},
  number = {1},
  pages = {1-15},
  doi = {10.11648/j.ijaas.20210701.11},
  url = {https://doi.org/10.11648/j.ijaas.20210701.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijaas.20210701.11},
  abstract = {Exploitation of phosphate solubilizing bacteria (PSB) and fungi as bioinoculants instead of chemical fertilizers is known to promote plant growth through the supply of plant nutrients. In view of this, the present investigation was planned to assess the phytobeneficial traits of phosphate solublizing bacterial and fungal isolates recovered from coffee arabica rhizosphere/ vermicompost and to determine their potential in growth promotion of coffee seedlings. They were isolated and purified following standard methods. A total number of 154 bacteria and 72 fungi were isolated from vermicompost and coffee rhizosphere. Out of these, twelve potent bacterial and nine fungal isolates were selected and investigated. Among twelve bacterial isolates, three of them showed significant potential to solubilize Ca3 (PO4)2 and had phytobeneficial traits, viz, indole acetic acid, NH3, HCN production and N-fixing ability. The three bacterial isolates (RCHVCB1, RScB1.19 and RMaB2.11) exhibited also remarkable tolerance to ecophysiological factors such as heavy metal, acidity and salinity. These potent isolates were selected for further identification based on morphological and biochemical characteristics and presumptively identified as genera of Pseudomonas (RCHVCB1) and Bacillus (RScB1.19 and RMaB2.11). Similarly, three fungal isolates with superior phosphate solubilizeation ability were characterized and identified as genera Penicillium (RSCF1.19) and Aspergillus (RCHVCF2 and RLVCF2). Consequently, these three efficient bacterial and fungal isolates were evaluated on the coffee seed germination on Petri dish based trial under laboratory condition. The results of inoculated seeds showed significant (p≤0.05) differences in germination rate and vigor index compared to the control. Among all inoculums RScB1.19, RMaB2.11+RSCF1.19 and RMaB2.11 + RLVCF2 showed significantly (p≤0.05) high germination rate (20.59%) over the control (13.33%). Moreover, a single inoculation of RLVCF2, RSCF1.19 and co-inoculation of RMaB2.11 with RLVCF2 also showed significant (p≤0.05) mean root length (1.31cm) and mean shoot length (1.48cm) over the control. These effective bacterial and fungal solublizers can be recommended under field condition as biofertilizer agent and reducing the cost required for chemical fertilizers and providing a step forward towards sustainable agriculture.},
 year = {2021}
}

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TY  - JOUR
T1  - Multi Traits of Phosphate Solublizing Bacterial and Fungal Isolates and Evaluation of Their Potential as Biofertilizer Agent for Coffee Production
AU  - Reshid Abafita Abawari
AU  - Fasil Asefa Tuji
AU  - Diriba Muleta Yadete
Y1  - 2021/01/12
PY  - 2021
N1  - https://doi.org/10.11648/j.ijaas.20210701.11
DO  - 10.11648/j.ijaas.20210701.11
T2  - International Journal of Applied Agricultural Sciences
JF  - International Journal of Applied Agricultural Sciences
JO  - International Journal of Applied Agricultural Sciences
SP  - 1
EP  - 15
PB  - Science Publishing Group
SN  - 2469-7885
UR  - https://doi.org/10.11648/j.ijaas.20210701.11
AB  - Exploitation of phosphate solubilizing bacteria (PSB) and fungi as bioinoculants instead of chemical fertilizers is known to promote plant growth through the supply of plant nutrients. In view of this, the present investigation was planned to assess the phytobeneficial traits of phosphate solublizing bacterial and fungal isolates recovered from coffee arabica rhizosphere/ vermicompost and to determine their potential in growth promotion of coffee seedlings. They were isolated and purified following standard methods. A total number of 154 bacteria and 72 fungi were isolated from vermicompost and coffee rhizosphere. Out of these, twelve potent bacterial and nine fungal isolates were selected and investigated. Among twelve bacterial isolates, three of them showed significant potential to solubilize Ca3 (PO4)2 and had phytobeneficial traits, viz, indole acetic acid, NH3, HCN production and N-fixing ability. The three bacterial isolates (RCHVCB1, RScB1.19 and RMaB2.11) exhibited also remarkable tolerance to ecophysiological factors such as heavy metal, acidity and salinity. These potent isolates were selected for further identification based on morphological and biochemical characteristics and presumptively identified as genera of Pseudomonas (RCHVCB1) and Bacillus (RScB1.19 and RMaB2.11). Similarly, three fungal isolates with superior phosphate solubilizeation ability were characterized and identified as genera Penicillium (RSCF1.19) and Aspergillus (RCHVCF2 and RLVCF2). Consequently, these three efficient bacterial and fungal isolates were evaluated on the coffee seed germination on Petri dish based trial under laboratory condition. The results of inoculated seeds showed significant (p≤0.05) differences in germination rate and vigor index compared to the control. Among all inoculums RScB1.19, RMaB2.11+RSCF1.19 and RMaB2.11 + RLVCF2 showed significantly (p≤0.05) high germination rate (20.59%) over the control (13.33%). Moreover, a single inoculation of RLVCF2, RSCF1.19 and co-inoculation of RMaB2.11 with RLVCF2 also showed significant (p≤0.05) mean root length (1.31cm) and mean shoot length (1.48cm) over the control. These effective bacterial and fungal solublizers can be recommended under field condition as biofertilizer agent and reducing the cost required for chemical fertilizers and providing a step forward towards sustainable agriculture.
VL  - 7
IS  - 1
ER  -

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Author Information

Reshid Abafita Abawari

Department of Microbial, Cellular and Molecular Biology, Jimma Agricultural Research Centre, Jimma, Ethiopia
Fasil Asefa Tuji

Department of Microbial, Cellular and Molecular Biology, Addis Ababa University, Addis Ababa, Ethiopia
Diriba Muleta Yadete

Institute of Biotechnology, Addis Ababa University, Addis Ababa, Ethiopia

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Reshid Abafita Abawari, Fasil Asefa Tuji, Diriba Muleta Yadete. (2021). Multi Traits of Phosphate Solublizing Bacterial and Fungal Isolates and Evaluation of Their Potential as Biofertilizer Agent for Coffee Production. International Journal of Applied Agricultural Sciences, 7(1), 1-15. https://doi.org/10.11648/j.ijaas.20210701.11

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Reshid Abafita Abawari; Fasil Asefa Tuji; Diriba Muleta Yadete. Multi Traits of Phosphate Solublizing Bacterial and Fungal Isolates and Evaluation of Their Potential as Biofertilizer Agent for Coffee Production. Int. J. Appl. Agric. Sci. 2021, 7(1), 1-15. doi: 10.11648/j.ijaas.20210701.11

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Reshid Abafita Abawari, Fasil Asefa Tuji, Diriba Muleta Yadete. Multi Traits of Phosphate Solublizing Bacterial and Fungal Isolates and Evaluation of Their Potential as Biofertilizer Agent for Coffee Production. Int J Appl Agric Sci. 2021;7(1):1-15. doi: 10.11648/j.ijaas.20210701.11

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@article{10.11648/j.ijaas.20210701.11,
  author = {Reshid Abafita Abawari and Fasil Asefa Tuji and Diriba Muleta Yadete},
  title = {Multi Traits of Phosphate Solublizing Bacterial and Fungal Isolates and Evaluation of Their Potential as Biofertilizer Agent for Coffee Production},
  journal = {International Journal of Applied Agricultural Sciences},
  volume = {7},
  number = {1},
  pages = {1-15},
  doi = {10.11648/j.ijaas.20210701.11},
  url = {https://doi.org/10.11648/j.ijaas.20210701.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijaas.20210701.11},
  abstract = {Exploitation of phosphate solubilizing bacteria (PSB) and fungi as bioinoculants instead of chemical fertilizers is known to promote plant growth through the supply of plant nutrients. In view of this, the present investigation was planned to assess the phytobeneficial traits of phosphate solublizing bacterial and fungal isolates recovered from coffee arabica rhizosphere/ vermicompost and to determine their potential in growth promotion of coffee seedlings. They were isolated and purified following standard methods. A total number of 154 bacteria and 72 fungi were isolated from vermicompost and coffee rhizosphere. Out of these, twelve potent bacterial and nine fungal isolates were selected and investigated. Among twelve bacterial isolates, three of them showed significant potential to solubilize Ca3 (PO4)2 and had phytobeneficial traits, viz, indole acetic acid, NH3, HCN production and N-fixing ability. The three bacterial isolates (RCHVCB1, RScB1.19 and RMaB2.11) exhibited also remarkable tolerance to ecophysiological factors such as heavy metal, acidity and salinity. These potent isolates were selected for further identification based on morphological and biochemical characteristics and presumptively identified as genera of Pseudomonas (RCHVCB1) and Bacillus (RScB1.19 and RMaB2.11). Similarly, three fungal isolates with superior phosphate solubilizeation ability were characterized and identified as genera Penicillium (RSCF1.19) and Aspergillus (RCHVCF2 and RLVCF2). Consequently, these three efficient bacterial and fungal isolates were evaluated on the coffee seed germination on Petri dish based trial under laboratory condition. The results of inoculated seeds showed significant (p≤0.05) differences in germination rate and vigor index compared to the control. Among all inoculums RScB1.19, RMaB2.11+RSCF1.19 and RMaB2.11 + RLVCF2 showed significantly (p≤0.05) high germination rate (20.59%) over the control (13.33%). Moreover, a single inoculation of RLVCF2, RSCF1.19 and co-inoculation of RMaB2.11 with RLVCF2 also showed significant (p≤0.05) mean root length (1.31cm) and mean shoot length (1.48cm) over the control. These effective bacterial and fungal solublizers can be recommended under field condition as biofertilizer agent and reducing the cost required for chemical fertilizers and providing a step forward towards sustainable agriculture.},
 year = {2021}
}

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TY  - JOUR
T1  - Multi Traits of Phosphate Solublizing Bacterial and Fungal Isolates and Evaluation of Their Potential as Biofertilizer Agent for Coffee Production
AU  - Reshid Abafita Abawari
AU  - Fasil Asefa Tuji
AU  - Diriba Muleta Yadete
Y1  - 2021/01/12
PY  - 2021
N1  - https://doi.org/10.11648/j.ijaas.20210701.11
DO  - 10.11648/j.ijaas.20210701.11
T2  - International Journal of Applied Agricultural Sciences
JF  - International Journal of Applied Agricultural Sciences
JO  - International Journal of Applied Agricultural Sciences
SP  - 1
EP  - 15
PB  - Science Publishing Group
SN  - 2469-7885
UR  - https://doi.org/10.11648/j.ijaas.20210701.11
AB  - Exploitation of phosphate solubilizing bacteria (PSB) and fungi as bioinoculants instead of chemical fertilizers is known to promote plant growth through the supply of plant nutrients. In view of this, the present investigation was planned to assess the phytobeneficial traits of phosphate solublizing bacterial and fungal isolates recovered from coffee arabica rhizosphere/ vermicompost and to determine their potential in growth promotion of coffee seedlings. They were isolated and purified following standard methods. A total number of 154 bacteria and 72 fungi were isolated from vermicompost and coffee rhizosphere. Out of these, twelve potent bacterial and nine fungal isolates were selected and investigated. Among twelve bacterial isolates, three of them showed significant potential to solubilize Ca3 (PO4)2 and had phytobeneficial traits, viz, indole acetic acid, NH3, HCN production and N-fixing ability. The three bacterial isolates (RCHVCB1, RScB1.19 and RMaB2.11) exhibited also remarkable tolerance to ecophysiological factors such as heavy metal, acidity and salinity. These potent isolates were selected for further identification based on morphological and biochemical characteristics and presumptively identified as genera of Pseudomonas (RCHVCB1) and Bacillus (RScB1.19 and RMaB2.11). Similarly, three fungal isolates with superior phosphate solubilizeation ability were characterized and identified as genera Penicillium (RSCF1.19) and Aspergillus (RCHVCF2 and RLVCF2). Consequently, these three efficient bacterial and fungal isolates were evaluated on the coffee seed germination on Petri dish based trial under laboratory condition. The results of inoculated seeds showed significant (p≤0.05) differences in germination rate and vigor index compared to the control. Among all inoculums RScB1.19, RMaB2.11+RSCF1.19 and RMaB2.11 + RLVCF2 showed significantly (p≤0.05) high germination rate (20.59%) over the control (13.33%). Moreover, a single inoculation of RLVCF2, RSCF1.19 and co-inoculation of RMaB2.11 with RLVCF2 also showed significant (p≤0.05) mean root length (1.31cm) and mean shoot length (1.48cm) over the control. These effective bacterial and fungal solublizers can be recommended under field condition as biofertilizer agent and reducing the cost required for chemical fertilizers and providing a step forward towards sustainable agriculture.
VL  - 7
IS  - 1
ER  -

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