Estimation of Nitrogen Content and Marketable Yield Using Chlorophyll Readings

Xiaoyan Pan; Hongxia Cao; Jiankai Zhang; Zijian He; Bingjie Du

doi:doi:10.11648/j.ijaas.20210705.12

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Estimation of Nitrogen Content and Marketable Yield Using Chlorophyll Readings

Xiaoyan Pan, Hongxia Cao, Jiankai Zhang, Zijian He, Bingjie Du

Published in International Journal of Applied Agricultural Sciences (Volume 7, Issue 5)

Received: 10 September 2021 Accepted: 26 September 2021 Published: 12 October 2021

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Abstract

Accurate estimation of nitrogen content and yield is crucial and these can be predicted through chlorophyll readings as indicators. To explore the diagnosis model, a field experiment was conducted in a greenhouse at Yang ling, northwest China. The SPAD values results estimated the nitrogen status, total marketable yield, and rates of reduction in market yield. There was a positive correlation between SPAD value at the middle leaf (MD-SPAD) and nitrogen accumulation, and between SPAD value under the leaf (UD-SPAD) and nitrogen accumulation (R²=0.37-0.78, P<0.01, n=27). The MD-SPAD and UD-SPAD were positively correlated with total marketable yield during the growth period (R²=0.43-0.59, P<0.01, n=27). However, a poor correlation was observed at the upper leaf (UP-SPAD) in the expanding fruit period but was better in the mature period. Moreover, the nitrogen nutrition index (NNI) and MD-SPAD were positively correlated (R²=0.42-0.55, P<0.01, n=54). There SPAD values and reduction rates of ranks were positively correlated in marketable yield (R²=0.48-0.52, P<0.01, n=54), including middle fruit yield (MFY) and big fruit yield (BFY). This was similar between SPAD values and reduction rates of total marketable yield (R²=50, P<0.01, n=54). This indicated that nitrogen accumulation estimates including nitrogen accumulation in stem, leaf, fruit, and the overall nitrogen accumulation, as well as total marketable yield, is not accurate via UP-SPAD during fruit expanding period but it is opposite in mature period. When simultaneously considering estimation of nitrogen accumulation, NNI and marketable yield using chlorophyll readings, which were adopted by mean of SPAD values the different leaf positions or SPAD values at the middle leaf. On the other hand, UP-SPAD should be cautiously used to estimate nitrogen status, yield, and yield reduction rates. The overall marketable yield was optimal under W2 of 2488 m³hm^-2 and N2 of 300 kghm^-2. The reduction rates during middle fruit yield, big fruit yield, and total marketable yield ranged from 31%-38%. The big fruit yield was the highest, indicating that the big fruit yield is highly affected by severe water stress in the blossom and fruit period.

Published in	International Journal of Applied Agricultural Sciences (Volume 7, Issue 5)
DOI	10.11648/j.ijaas.20210705.12
Page(s)	219-231
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Nitrogen Status, SPAD Value, Relationship, Leaf Position, Marketable Yield

References

[1]	Jiang Hui-Min, Z. J. S. X. and Jun-Cheng, A. Y. 2012. Responses of Agronomic Benefit and Soil Quality to Better Management of Nitrogen Fertilizer Application in Greenhouse Vegetable Land. Soil Science Society of China. 5, 650–660.
[2]	Xu, J. et al. 2020. Exploring optimal irrigation and nitrogen fertilization in a winter wheat-summer maize rotation system for improving crop yield and reducing water and nitrogen leaching. Agricultural Water Management. 228, 105904.
[3]	Li, Y.; Sun, Y.; Liao, S.; Zou, G.; Zhao, T.; Chen, Y.; Yang, J.; Zhang, L. 2017. Effects of two slow-release nitrogen fertilizers and irrigation on yield, quality, and water-fertilizer productivity of greenhouse tomato. Agricultural Water Management. 186, 139-146.
[4]	Badr, M. A.; Abou-Hussein, S. D.; El-Tohamy, W. A. 2016. Tomato yield, nitrogen uptake and water use efficiency as affected by planting geometry and level of nitrogen in an arid region. Agricultural Water Management. 169, 90-97.
[5]	Li, J.; Wang, Y.; Zhang, M.; Liu, Y.; Xu, X.; Lin, G.; Wang, Z.; Yang, Y.; Zhang, Y. 2019. Optimized micro-sprinkling irrigation scheduling improves grain yield by increasing the uptake and utilization of water and nitrogen during grain filling in winter wheat. Agricultural Water Management. 211, 59-69.
[6]	Zhang, M.; Dong, B.; Qiao, Y.; Shi, C.; Yang, H.; Wang, Y.; LIU, M. 2018. Yield and water use responses of winter wheat to irrigation and nitrogen application in the North China Plain. Journal of Integrative Agriculture. 17, 1194-1206.
[7]	Zhang, H.; Chi, D.; Wang, Q.; Fang, J.; Fang, X. 2011. Yield and Quality Response of Cucumber to Irrigation and Nitrogen Fertilization Under Subsurface Drip Irrigation in Solar Greenhouse. Agricultural Sciences in China. 10, 921-930.
[8]	Sun, Y.; Zhang, J.; Wang, H.; Wang, L.; Li, H. 2019. Identifying optimal water and nitrogen inputs for high efficiency and low environment impacts of a greenhouse summer cucumber with a model method. Agricultural Water Manage. 212, 23-34.
[9]	Peng, S.; Garcia, F. V.; Laza, R. C.; Sanico, A. L.; Visperas, R. M.; Cassman, K. G. 1996. Increased N-use efficiency using a chlorophyll meter on high-yielding irrigated rice. Field Crops Research. 47, 243-252.
[10]	Zheng, H.; Liu, Y.; Qin, Y.; Chen, Y.; Fan, M. 2015. Establishing dynamic thresholds for potato nitrogen status diagnosis with the SPAD chlorophyll meter. Journal of Integrative Agriculture. 14, 190-195.
[11]	Huang, L.; Yu, J.; Yang, J.; Zhang, R.; Bai, Y.; Sun, C.; Zhuang, H. 2016. Relationships Between Yield, Quality and Nitrogen Uptake and Utilization of Organically Grown Rice Varieties. Pedosphere. 26, 85-97.
[12]	Gholizadeh, A.; Saberioon, M.; Borůvka, L.; Wayayok, A.; Mohd Soom, M. A. 2017. Leaf chlorophyll and nitrogen dynamics and their relationship to lowland rice yield for site-specific paddy management. Information Processing in Agriculture. 4, 259-268.
[13]	Zhao, B.; Liu, Z.; Ata-Ul-Karim, S. T.; Xiao, J.; Liu, Z.; Qi, A.; Ning, D.; Nan, J.; Duan, A. 2016. Rapid and nondestructive estimation of the nitrogen nutrition index in winter barley using chlorophyll measurements. Field Crops Research. 185, 59-68.
[14]	Liu, S.; Li, L.; Gao, W.; Zhang, Y.; Liu, Y.; Wang, S.; Lu, J. 2018. Diagnosis of nitrogen status in winter oilseed rape (Brassica napus L.) using in-situ hyperspectral data and unmanned aerial vehicle (UAV) multispectral images. Computers and Electronics in Agriculture. 151, 185-195.
[15]	Ravier, C.; Quemada, M.; Jeuffroy, M. 2017. Use of a chlorophyll meter to assess nitrogen nutrition index during the growth cycle in winter wheat. Field Crops Research. 214, 73-82.
[16]	Xiong, D.; Chen, J.; Yu, T.; Gao, W.; Ling, X.; Li, Y.; Peng, S.; Huang, J. 2015. SPAD-based leaf nitrogen estimation is impacted by environmental factors and crop leaf characteristics. Scientific Reports. 5, 13389.
[17]	Yuan, Z.; Ata-Ul-Karim, S. T.; Cao, Q.; Lu, Z.; Cao, W.; Zhu, Y.; Liu, X. 2016. Indicators for diagnosing nitrogen status of rice based on chlorophyll meter readings. Field Crops Research. 185, 12-20.
[18]	Bauerle, W. L.; Weston, D. J.; Bowden, J. D.; Dudley, J. B.; Toler, J. E. 2004. Leaf absorptance of photosynthetically active radiation in relation to chlorophyll meter estimates among woody plant species. Scientia Horticulturae. 101, 169-178.
[19]	Hawkins, T. S.; Gardiner, E. S.; Comer, G. S. 2009. Modeling the relationship between extractable chlorophyll and SPAD-502 readings for endangered plant species research. Journal for Nature Conservation. 17, 123-127.
[20]	Puangbut, D.; Jogloy, S.; Vorasoot, N. 2017. Association of photosynthetic traits with water use efficiency and SPAD chlorophyll meter reading of Jerusalem artichoke under drought conditions. Agricultural Water Manage. 188, 29-35.
[21]	Amirruddin, A. D.; Muharam, F. M.; Ismail, M. H.; Ismail, M. F.; Tan, N. P.; Karam, D. S. 2020. Hyperspectral remote sensing for assessment of chlorophyll sufficiency levels in mature oil palm (Elaeis guineensis) based on frond numbers: Analysis of decision tree and random forest. Computers and Electronics in Agriculture. 169, 105221.
[22]	Ahmad, I.; Dole, J. M.; Nelson, P. 2012. Nitrogen application rate, leaf position and age affect leaf nutrient status of five specialty cut flowers. Scientia Horticulturae. 142, 14-22.
[23]	Padilla, F. M.; Peña-Fleitas, M. T.; Gallardo, M.; Giménez, C.; Thompson, R. B. 2017. Derivation of sufficiency values of a chlorophyll meter to estimate cucumber nitrogen status and yield. Computers and Electronics in Agriculture. 141, 54-64.
[24]	Lemaire, G.; Denoix, A. 1987. Croissance estivale en matière sèche de peuplements de fétuque élevée (Festuca arundinacea Schreb.) et de dactyle (Dactylis glomerata L.) dans l'Ouest de la France. II. Interaction entre les niveaux d'alimentation hydrique et de nutrition azotée. Agronomie. 7, 381-389.
[25]	Shaobing Peng, F. V. G. R. 1993. Adjustment for Specific Leaf Weight Improves Chlorophyll Meter‘sEstimate of Rice Leaf Nitrogen Concentration. Agron Journal. 85, 987-990.
[26]	F. M. Padilla, M. T. P. M. 2015. Threshold values of canopy reflectance indices and chlorophyll meter readings for optimal nitrogen nutrition of tomato. Annals of Applied Biology ISSN. 166, 271-285.
[27]	Padilla, F. M.; Peña-Fleitas, M. T.; Gallardo, M.; Thompson, R. B. 2016. Proximal optical sensing of cucumber crop N status using chlorophyll fluorescence indices. European Journal of Agronomy. 73, 83-97.
[28]	Li, Y.; Song, H.; Zhou, L.; Xu, Z.; Zhou, G. 2019. Tracking chlorophyll fluorescence as an indicator of drought and rewatering across the entire leaf lifespan in a maize field. Agricultural Water Manage. 211, 190-201.
[29]	Hoel, B. O. 1998. Use of a hand-held chlorophyll meter in winter wheat: Evaluation of different measuring positions on the leaves. Acta Agriculturae Scandinavica, Section B - Soil & Plant Science. 48, 222-228.
[30]	Bavec, F.; Bavec, M. 2001. Chlorophyll meter readings of winter wheat cultivars and grain yield prediction. Communications in Soil Science and Plant Analysis. 32, 2709-2719.
[31]	De, C.; Neto, O.; Lobato, A.; Gonçalves-Vidigal, C.; Cezar, R.; Costa, L.; Gomes, B.; Filho, B.; Alves, G.; Mello, W., et al. 2009. Carbon compounds and chlorophyll contents in sorghum submitted to water deficit during three growth stages. Journal of Food Agriculture and Environment. 7, 588-593.32.
[32]	Sarijeva, G.; Knapp, M.; Lichtenthaler, H. K. 2007. Differences in photosynthetic activity, chlorophyll and carotenoid levels, and in chlorophyll fluorescence parameters in green sun and shade leaves of Ginkgo and Fagus. Journal of Plant Physiology. 164.950-955.
[33]	Talwar, H. S.; Surwenshi, A.; Seetharama, N. 2009. Use of SPAD chlorophyll meter to screen sorghum (Sorghum bicolor) lines for postflowering drought tolerance. Indian Journal of Agricultural Sciences. 79, 35-39.
[34]	Topcu, S.; Kirda, C.; Dasgan, Y.; Kaman, H.; Cetin, M.; Yazici, A.; Bacon, M. A. 2007. Yield response and N-fertiliser recovery of tomato grown under deficit irrigation. European Journal of Agronomy. 26, 64-70.
[35]	Wu, H.; Du, S.; Zhang, Y.; An, J.; Zou, H.; Zhang, Y.; Yu, N. 2019. Effects of irrigation and nitrogen fertilization on greenhouse soil organic nitrogen fractions and soil-soluble nitrogen pools. Agricultural Water Manage. 216, 415-424.
[36]	Du, Y.; Cao, H.; Liu, S.; Gu, X.; Cao, Y. Response of yield, quality, water and nitrogen use efficiency of tomato to different levels of water and nitrogen under drip irrigation in Northwestern China. J Integr Agr 2017, 16, 1153-1161.
[37]	Fullana-Pericàs, M.; Conesa, M. À.; Douthe, C.; El Aou-ouad, H.; Ribas-Carbó, M.; Galmés, J. 2019. Tomato landraces as a source to minimize yield losses and improve fruit quality under water deficit conditions. Agricultural Water Manage. 223, 105722.
[38]	Wang, F.; Kang, S.; Du, T.; Li, F.; Qiu, R. 2011. Determination of comprehensive quality index for tomato and its response to different irrigation treatments. Agricultural Water Management. 98, 1228-1238.
[39]	Chen, J.; Kang, S.; Du, T.; Qiu, R.; Guo, P.; Chen, R. 2013. Quantitative response of greenhouse tomato yield and quality to water deficit at different growth stages. Agricultural Water Management. 129, 152-162.
[40]	Jiang, X.; Zhao, Y.; Tong, L.; Wang, R.; Zhao, S. 2019. Quantitative Analysis of Tomato Yield and Comprehensive Fruit Quality in Response to Deficit Irrigation at Different Growth Stages. Hortscience. 54, 1409-1417.

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APA Style

Xiaoyan Pan, Hongxia Cao, Jiankai Zhang, Zijian He, Bingjie Du. (2021). Estimation of Nitrogen Content and Marketable Yield Using Chlorophyll Readings. International Journal of Applied Agricultural Sciences, 7(5), 219-231. https://doi.org/10.11648/j.ijaas.20210705.12

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ACS Style

Xiaoyan Pan; Hongxia Cao; Jiankai Zhang; Zijian He; Bingjie Du. Estimation of Nitrogen Content and Marketable Yield Using Chlorophyll Readings. Int. J. Appl. Agric. Sci. 2021, 7(5), 219-231. doi: 10.11648/j.ijaas.20210705.12

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AMA Style

Xiaoyan Pan, Hongxia Cao, Jiankai Zhang, Zijian He, Bingjie Du. Estimation of Nitrogen Content and Marketable Yield Using Chlorophyll Readings. Int J Appl Agric Sci. 2021;7(5):219-231. doi: 10.11648/j.ijaas.20210705.12

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@article{10.11648/j.ijaas.20210705.12,
  author = {Xiaoyan Pan and Hongxia Cao and Jiankai Zhang and Zijian He and Bingjie Du},
  title = {Estimation of Nitrogen Content and Marketable Yield Using Chlorophyll Readings},
  journal = {International Journal of Applied Agricultural Sciences},
  volume = {7},
  number = {5},
  pages = {219-231},
  doi = {10.11648/j.ijaas.20210705.12},
  url = {https://doi.org/10.11648/j.ijaas.20210705.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijaas.20210705.12},
  abstract = {Accurate estimation of nitrogen content and yield is crucial and these can be predicted through chlorophyll readings as indicators. To explore the diagnosis model, a field experiment was conducted in a greenhouse at Yang ling, northwest China. The SPAD values results estimated the nitrogen status, total marketable yield, and rates of reduction in market yield. There was a positive correlation between SPAD value at the middle leaf (MD-SPAD) and nitrogen accumulation, and between SPAD value under the leaf (UD-SPAD) and nitrogen accumulation (R2=0.37-0.78, P2=0.43-0.59, P2=0.42-0.55, P2=0.48-0.52, P2=50, P3hm-2 and N2 of 300 kghm-2. The reduction rates during middle fruit yield, big fruit yield, and total marketable yield ranged from 31%-38%. The big fruit yield was the highest, indicating that the big fruit yield is highly affected by severe water stress in the blossom and fruit period.},
 year = {2021}
}

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TY  - JOUR
T1  - Estimation of Nitrogen Content and Marketable Yield Using Chlorophyll Readings
AU  - Xiaoyan Pan
AU  - Hongxia Cao
AU  - Jiankai Zhang
AU  - Zijian He
AU  - Bingjie Du
Y1  - 2021/10/12
PY  - 2021
N1  - https://doi.org/10.11648/j.ijaas.20210705.12
DO  - 10.11648/j.ijaas.20210705.12
T2  - International Journal of Applied Agricultural Sciences
JF  - International Journal of Applied Agricultural Sciences
JO  - International Journal of Applied Agricultural Sciences
SP  - 219
EP  - 231
PB  - Science Publishing Group
SN  - 2469-7885
UR  - https://doi.org/10.11648/j.ijaas.20210705.12
AB  - Accurate estimation of nitrogen content and yield is crucial and these can be predicted through chlorophyll readings as indicators. To explore the diagnosis model, a field experiment was conducted in a greenhouse at Yang ling, northwest China. The SPAD values results estimated the nitrogen status, total marketable yield, and rates of reduction in market yield. There was a positive correlation between SPAD value at the middle leaf (MD-SPAD) and nitrogen accumulation, and between SPAD value under the leaf (UD-SPAD) and nitrogen accumulation (R2=0.37-0.78, P2=0.43-0.59, P2=0.42-0.55, P2=0.48-0.52, P2=50, P3hm-2 and N2 of 300 kghm-2. The reduction rates during middle fruit yield, big fruit yield, and total marketable yield ranged from 31%-38%. The big fruit yield was the highest, indicating that the big fruit yield is highly affected by severe water stress in the blossom and fruit period.
VL  - 7
IS  - 5
ER  -

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Author Information

Xiaoyan Pan

Key Laboratory of Agricultural Soil and Water Engineering in Arid and Semiarid Areas, Ministry of Education, Northwest Agriculture & Forestry University, Yangling, China
Hongxia Cao

Key Laboratory of Agricultural Soil and Water Engineering in Arid and Semiarid Areas, Ministry of Education, Northwest Agriculture & Forestry University, Yangling, China
Jiankai Zhang

Key Laboratory of Agricultural Soil and Water Engineering in Arid and Semiarid Areas, Ministry of Education, Northwest Agriculture & Forestry University, Yangling, China
Zijian He

Key Laboratory of Agricultural Soil and Water Engineering in Arid and Semiarid Areas, Ministry of Education, Northwest Agriculture & Forestry University, Yangling, China
Bingjie Du

Key Laboratory of Agricultural Soil and Water Engineering in Arid and Semiarid Areas, Ministry of Education, Northwest Agriculture & Forestry University, Yangling, China

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APA Style

Xiaoyan Pan, Hongxia Cao, Jiankai Zhang, Zijian He, Bingjie Du. (2021). Estimation of Nitrogen Content and Marketable Yield Using Chlorophyll Readings. International Journal of Applied Agricultural Sciences, 7(5), 219-231. https://doi.org/10.11648/j.ijaas.20210705.12

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ACS Style

Xiaoyan Pan; Hongxia Cao; Jiankai Zhang; Zijian He; Bingjie Du. Estimation of Nitrogen Content and Marketable Yield Using Chlorophyll Readings. Int. J. Appl. Agric. Sci. 2021, 7(5), 219-231. doi: 10.11648/j.ijaas.20210705.12

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AMA Style

Xiaoyan Pan, Hongxia Cao, Jiankai Zhang, Zijian He, Bingjie Du. Estimation of Nitrogen Content and Marketable Yield Using Chlorophyll Readings. Int J Appl Agric Sci. 2021;7(5):219-231. doi: 10.11648/j.ijaas.20210705.12

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@article{10.11648/j.ijaas.20210705.12,
  author = {Xiaoyan Pan and Hongxia Cao and Jiankai Zhang and Zijian He and Bingjie Du},
  title = {Estimation of Nitrogen Content and Marketable Yield Using Chlorophyll Readings},
  journal = {International Journal of Applied Agricultural Sciences},
  volume = {7},
  number = {5},
  pages = {219-231},
  doi = {10.11648/j.ijaas.20210705.12},
  url = {https://doi.org/10.11648/j.ijaas.20210705.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijaas.20210705.12},
  abstract = {Accurate estimation of nitrogen content and yield is crucial and these can be predicted through chlorophyll readings as indicators. To explore the diagnosis model, a field experiment was conducted in a greenhouse at Yang ling, northwest China. The SPAD values results estimated the nitrogen status, total marketable yield, and rates of reduction in market yield. There was a positive correlation between SPAD value at the middle leaf (MD-SPAD) and nitrogen accumulation, and between SPAD value under the leaf (UD-SPAD) and nitrogen accumulation (R2=0.37-0.78, P2=0.43-0.59, P2=0.42-0.55, P2=0.48-0.52, P2=50, P3hm-2 and N2 of 300 kghm-2. The reduction rates during middle fruit yield, big fruit yield, and total marketable yield ranged from 31%-38%. The big fruit yield was the highest, indicating that the big fruit yield is highly affected by severe water stress in the blossom and fruit period.},
 year = {2021}
}

Copy | Download

TY  - JOUR
T1  - Estimation of Nitrogen Content and Marketable Yield Using Chlorophyll Readings
AU  - Xiaoyan Pan
AU  - Hongxia Cao
AU  - Jiankai Zhang
AU  - Zijian He
AU  - Bingjie Du
Y1  - 2021/10/12
PY  - 2021
N1  - https://doi.org/10.11648/j.ijaas.20210705.12
DO  - 10.11648/j.ijaas.20210705.12
T2  - International Journal of Applied Agricultural Sciences
JF  - International Journal of Applied Agricultural Sciences
JO  - International Journal of Applied Agricultural Sciences
SP  - 219
EP  - 231
PB  - Science Publishing Group
SN  - 2469-7885
UR  - https://doi.org/10.11648/j.ijaas.20210705.12
AB  - Accurate estimation of nitrogen content and yield is crucial and these can be predicted through chlorophyll readings as indicators. To explore the diagnosis model, a field experiment was conducted in a greenhouse at Yang ling, northwest China. The SPAD values results estimated the nitrogen status, total marketable yield, and rates of reduction in market yield. There was a positive correlation between SPAD value at the middle leaf (MD-SPAD) and nitrogen accumulation, and between SPAD value under the leaf (UD-SPAD) and nitrogen accumulation (R2=0.37-0.78, P2=0.43-0.59, P2=0.42-0.55, P2=0.48-0.52, P2=50, P3hm-2 and N2 of 300 kghm-2. The reduction rates during middle fruit yield, big fruit yield, and total marketable yield ranged from 31%-38%. The big fruit yield was the highest, indicating that the big fruit yield is highly affected by severe water stress in the blossom and fruit period.
VL  - 7
IS  - 5
ER  -

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