Isolation and Molecular Characterization of Mycobiota and Other Microbiota from Fingernails

Adeoti Olatunde Micheal; Ponle Temitope Elisabeth; Komolafe Kafilat Adenike; Adedokun Elisabeth Olajumoke; Abiola Adebiyi; Olufemi Samson Olutope; Adesina David Ademola

doi:doi:10.11648/j.ijcda.20210701.11

Isolation and Molecular Characterization of Mycobiota and Other Microbiota from Fingernails

Adeoti Olatunde Micheal, Ponle Temitope Elisabeth, Komolafe Kafilat Adenike, Adedokun Elisabeth Olajumoke, Abiola Adebiyi, Olufemi Samson Olutope, Adesina David Ademola

Published in International Journal of Clinical and Developmental Anatomy (Volume 7, Issue 1)

Received: 29 January 2021 Accepted: 27 February 2021 Published: 9 March 2021

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

This study was carried out to ascertain the hygienic practices among tertiary institutions study and also to isolate and identify microbiota from their thereby conduct molecular screening of fingernails for potential pathogens. A total of 300 nail samples carefully aseptically collected from 30 consented individuals include artificial and natural fingernails from both male and female students of The Oke-Ogun Polytechnic, Saki. The students’ consent were sought for and other ethical issues were complied with as stipulated by the Institutional Ethical Committee. The fingernails were swabbed with sterile swab sticks and thereafter inoculated on the surface of suitably prepared media plates and sub-cultured to obtain pure isolates. Morphological and biochemical tests were carried out on the isolates to confirm the isolates. All students were found to harbour diverse microbes on their undernails. The microorganisms isolated include: E.coli 16.6%, Staphylococcus aureus 22.2%, Streptococcus spp 13.88%, Bacillus spp 25%, members of the Acinetobacter 2.77%, Salmonella spp 13.88%, Fungi 5.55%. The suspected Fungi isolates were sent for sequencing for further identification and characterization. The highest prevalence was found to be more in females than in their male counterparts, which could be as a result of unhygienic practices especially nail-fixing related of artificial nails because those with artificial nails keep longer nails than keeping natural nails. This result further confirmed that fingernails are a possible reservoir of several microbes and could be implicated in the spread of more virulent microorganisms.

Published in	International Journal of Clinical and Developmental Anatomy (Volume 7, Issue 1)
DOI	10.11648/j.ijcda.20210701.11
Page(s)	1-5
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Molecular Screening, Virulent, Artificial Nails, Microbiota, Fingernails, Unhygienic-practices

References

[1]	Bean N. H., Goulding J. S, Lao, C and Angulo, F. J. (1996) Surveillance and food borne diseases outbreak, United States, Morbidity and Mortality Weekly Report. 45: 1-55. Res 3 (12): 2009-2012.
[2]	Ray S. K, Amarch and R, Srikanth J. and Majumdar K. K (2011). A study on the prevalence of bacteria in the hands of children and their perception on hand washing in two schools of Bangalore and Kolkota. Indian journal of public health, 55 (4): 293-297.
[3]	Patel H. R, Daniel P. S, Anand I. S and Patel C. N. (2010). Role of community pharmacist in assessing the awareness of hand hygiene in rural area. Journal of Global Pharma.
[4]	Wachukwu C. K, S. D Abbey, A. O Ollor and Obilor N. L (2007). Public health implication of artificial fingernails used by health workers and food handlers in Port Harcourt, Nigeria. Journal of Applied Sciences. 7: 3580-3583.
[5]	Hedderwick S. A, Mcweil S. A, Lyons M. J (2000) Pathogenic organisms associated with artificial fingernails worn by health care workers. Infect control Hospital Epidemiological. 21 (8) 505-509.
[6]	Prescott L. M Harley J. P, Klein D. A (2005). Microbiology 6^th ed. Tim Migram-Hill co. New Delhi PP: 675.
[7]	Langford RM, 2009. Handwashing and its impact on child health in Kathmandu, Nepal. Doctoral thesis, Durham University.
[8]	Lau CH, Spriston E. E, Sohn M, Mason I, Gadola E, Damitz M. Lin C, Wu F. M, Doyle M. P, Micheal B. S and Williams K. (2002). Removal of Escherichia coli on hands with natural and artificial fingernails. International J. Epidemiological 21: 1157-1164.
[9]	Larson E. 2001. Hygiene of the skin When is clean too clean? Journal of emerging infectious diseases. 7 (2) 12-17. Technology, 2 (5): 59-61.
[10]	Ray S. K, Dobe M, Lahiri A and Basu S. S (2009) Handwashing practices in urban and rural communities in and around Kolkota, West Bengal Indian J Public health 53: 1192-1195.
[11]	Cheesbrough M., (2006). District Laboratory Practice in Tropical Countries. 2^nd Edition. Cambridge University Press, Cambridge, (178-179.
[12]	Ramos J. L (2004) Pseudomonas New York: Kluwer Academic/plenum publishers, p.
[13]	Ekrakene T. And Igeleke C. L (2007). Microorganisms associated with public mobile phones along Benin-Sapele express way, Benin City, Edo state of Nigeria J. Appl. Sci.
[14]	Akinyemi K. O, Atapu A. D, Adetona O and Coker, A. O (2009). The potential role of mobile phones in the spread of bacterial infections, Journal. Infectious Dev Ctries. 318: 628-632.
[15]	Tambekar D. H, Shirshat S. D, Kakde S. R and Ambekar K. B. (2009) Hand hygiene and health: an epidemiological study of students in Amravati. Africa Infection Diseases 3 (1): 26-30.
[16]	Tambekar D. H and Shirshat S. D (2012) Minimization of illness absenteeism in primary students using low cost hygiene interventions. Journal of Health and Applied Sciences.

Cite This Article

Plain Text BibTeX RIS

APA Style

Adeoti Olatunde Micheal, Ponle Temitope Elisabeth, Komolafe Kafilat Adenike, Adedokun Elisabeth Olajumoke, Abiola Adebiyi, et al. (2021). Isolation and Molecular Characterization of Mycobiota and Other Microbiota from Fingernails. International Journal of Clinical and Developmental Anatomy, 7(1), 1-5. https://doi.org/10.11648/j.ijcda.20210701.11

Copy | Download

ACS Style

Adeoti Olatunde Micheal; Ponle Temitope Elisabeth; Komolafe Kafilat Adenike; Adedokun Elisabeth Olajumoke; Abiola Adebiyi, et al. Isolation and Molecular Characterization of Mycobiota and Other Microbiota from Fingernails. Int. J. Clin. Dev. Anat. 2021, 7(1), 1-5. doi: 10.11648/j.ijcda.20210701.11

Copy | Download

AMA Style

Adeoti Olatunde Micheal, Ponle Temitope Elisabeth, Komolafe Kafilat Adenike, Adedokun Elisabeth Olajumoke, Abiola Adebiyi, et al. Isolation and Molecular Characterization of Mycobiota and Other Microbiota from Fingernails. Int J Clin Dev Anat. 2021;7(1):1-5. doi: 10.11648/j.ijcda.20210701.11

Copy | Download

@article{10.11648/j.ijcda.20210701.11,
  author = {Adeoti Olatunde Micheal and Ponle Temitope Elisabeth and Komolafe Kafilat Adenike and Adedokun Elisabeth Olajumoke and Abiola Adebiyi and Olufemi Samson Olutope and Adesina David Ademola},
  title = {Isolation and Molecular Characterization of Mycobiota and Other Microbiota from Fingernails},
  journal = {International Journal of Clinical and Developmental Anatomy},
  volume = {7},
  number = {1},
  pages = {1-5},
  doi = {10.11648/j.ijcda.20210701.11},
  url = {https://doi.org/10.11648/j.ijcda.20210701.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcda.20210701.11},
  abstract = {This study was carried out to ascertain the hygienic practices among tertiary institutions study and also to isolate and identify microbiota from their thereby conduct molecular screening of fingernails for potential pathogens. A total of 300 nail samples carefully aseptically collected from 30 consented individuals include artificial and natural fingernails from both male and female students of The Oke-Ogun Polytechnic, Saki. The students’ consent were sought for and other ethical issues were complied with as stipulated by the Institutional Ethical Committee. The fingernails were swabbed with sterile swab sticks and thereafter inoculated on the surface of suitably prepared media plates and sub-cultured to obtain pure isolates. Morphological and biochemical tests were carried out on the isolates to confirm the isolates. All students were found to harbour diverse microbes on their undernails. The microorganisms isolated include: E.coli 16.6%, Staphylococcus aureus 22.2%, Streptococcus spp 13.88%, Bacillus spp 25%, members of the Acinetobacter 2.77%, Salmonella spp 13.88%, Fungi 5.55%. The suspected Fungi isolates were sent for sequencing for further identification and characterization. The highest prevalence was found to be more in females than in their male counterparts, which could be as a result of unhygienic practices especially nail-fixing related of artificial nails because those with artificial nails keep longer nails than keeping natural nails. This result further confirmed that fingernails are a possible reservoir of several microbes and could be implicated in the spread of more virulent microorganisms.},
 year = {2021}
}

Copy | Download

TY  - JOUR
T1  - Isolation and Molecular Characterization of Mycobiota and Other Microbiota from Fingernails
AU  - Adeoti Olatunde Micheal
AU  - Ponle Temitope Elisabeth
AU  - Komolafe Kafilat Adenike
AU  - Adedokun Elisabeth Olajumoke
AU  - Abiola Adebiyi
AU  - Olufemi Samson Olutope
AU  - Adesina David Ademola
Y1  - 2021/03/09
PY  - 2021
N1  - https://doi.org/10.11648/j.ijcda.20210701.11
DO  - 10.11648/j.ijcda.20210701.11
T2  - International Journal of Clinical and Developmental Anatomy
JF  - International Journal of Clinical and Developmental Anatomy
JO  - International Journal of Clinical and Developmental Anatomy
SP  - 1
EP  - 5
PB  - Science Publishing Group
SN  - 2469-8008
UR  - https://doi.org/10.11648/j.ijcda.20210701.11
AB  - This study was carried out to ascertain the hygienic practices among tertiary institutions study and also to isolate and identify microbiota from their thereby conduct molecular screening of fingernails for potential pathogens. A total of 300 nail samples carefully aseptically collected from 30 consented individuals include artificial and natural fingernails from both male and female students of The Oke-Ogun Polytechnic, Saki. The students’ consent were sought for and other ethical issues were complied with as stipulated by the Institutional Ethical Committee. The fingernails were swabbed with sterile swab sticks and thereafter inoculated on the surface of suitably prepared media plates and sub-cultured to obtain pure isolates. Morphological and biochemical tests were carried out on the isolates to confirm the isolates. All students were found to harbour diverse microbes on their undernails. The microorganisms isolated include: E.coli 16.6%, Staphylococcus aureus 22.2%, Streptococcus spp 13.88%, Bacillus spp 25%, members of the Acinetobacter 2.77%, Salmonella spp 13.88%, Fungi 5.55%. The suspected Fungi isolates were sent for sequencing for further identification and characterization. The highest prevalence was found to be more in females than in their male counterparts, which could be as a result of unhygienic practices especially nail-fixing related of artificial nails because those with artificial nails keep longer nails than keeping natural nails. This result further confirmed that fingernails are a possible reservoir of several microbes and could be implicated in the spread of more virulent microorganisms.
VL  - 7
IS  - 1
ER  -

Copy | Download

Author Information

Adeoti Olatunde Micheal

Department of Science Laboratory Technology, Microbiology Option, the Oke-Ogun Polytechnic, Nigeria
Ponle Temitope Elisabeth

Department of Science Laboratory Technology, Microbiology Option, the Oke-Ogun Polytechnic, Nigeria
Komolafe Kafilat Adenike

Department of Science Laboratory Technology, Microbiology Option, the Oke-Ogun Polytechnic, Nigeria
Adedokun Elisabeth Olajumoke

Department of Science Laboratory Technology, Microbiology Option, the Oke-Ogun Polytechnic, Nigeria
Abiola Adebiyi

Department of Science Laboratory Technology, Microbiology Option, the Oke-Ogun Polytechnic, Nigeria
Olufemi Samson Olutope

Department of Science Laboratory Technology, Microbiology Option, the Oke-Ogun Polytechnic, Nigeria
Adesina David Ademola

Department of Science Laboratory Technology, Microbiology Option, the Oke-Ogun Polytechnic, Nigeria

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Adeoti Olatunde Micheal, Ponle Temitope Elisabeth, Komolafe Kafilat Adenike, Adedokun Elisabeth Olajumoke, Abiola Adebiyi, et al. (2021). Isolation and Molecular Characterization of Mycobiota and Other Microbiota from Fingernails. International Journal of Clinical and Developmental Anatomy, 7(1), 1-5. https://doi.org/10.11648/j.ijcda.20210701.11

Copy | Download

ACS Style

Adeoti Olatunde Micheal; Ponle Temitope Elisabeth; Komolafe Kafilat Adenike; Adedokun Elisabeth Olajumoke; Abiola Adebiyi, et al. Isolation and Molecular Characterization of Mycobiota and Other Microbiota from Fingernails. Int. J. Clin. Dev. Anat. 2021, 7(1), 1-5. doi: 10.11648/j.ijcda.20210701.11

Copy | Download

AMA Style

Adeoti Olatunde Micheal, Ponle Temitope Elisabeth, Komolafe Kafilat Adenike, Adedokun Elisabeth Olajumoke, Abiola Adebiyi, et al. Isolation and Molecular Characterization of Mycobiota and Other Microbiota from Fingernails. Int J Clin Dev Anat. 2021;7(1):1-5. doi: 10.11648/j.ijcda.20210701.11

Copy | Download

@article{10.11648/j.ijcda.20210701.11,
  author = {Adeoti Olatunde Micheal and Ponle Temitope Elisabeth and Komolafe Kafilat Adenike and Adedokun Elisabeth Olajumoke and Abiola Adebiyi and Olufemi Samson Olutope and Adesina David Ademola},
  title = {Isolation and Molecular Characterization of Mycobiota and Other Microbiota from Fingernails},
  journal = {International Journal of Clinical and Developmental Anatomy},
  volume = {7},
  number = {1},
  pages = {1-5},
  doi = {10.11648/j.ijcda.20210701.11},
  url = {https://doi.org/10.11648/j.ijcda.20210701.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcda.20210701.11},
  abstract = {This study was carried out to ascertain the hygienic practices among tertiary institutions study and also to isolate and identify microbiota from their thereby conduct molecular screening of fingernails for potential pathogens. A total of 300 nail samples carefully aseptically collected from 30 consented individuals include artificial and natural fingernails from both male and female students of The Oke-Ogun Polytechnic, Saki. The students’ consent were sought for and other ethical issues were complied with as stipulated by the Institutional Ethical Committee. The fingernails were swabbed with sterile swab sticks and thereafter inoculated on the surface of suitably prepared media plates and sub-cultured to obtain pure isolates. Morphological and biochemical tests were carried out on the isolates to confirm the isolates. All students were found to harbour diverse microbes on their undernails. The microorganisms isolated include: E.coli 16.6%, Staphylococcus aureus 22.2%, Streptococcus spp 13.88%, Bacillus spp 25%, members of the Acinetobacter 2.77%, Salmonella spp 13.88%, Fungi 5.55%. The suspected Fungi isolates were sent for sequencing for further identification and characterization. The highest prevalence was found to be more in females than in their male counterparts, which could be as a result of unhygienic practices especially nail-fixing related of artificial nails because those with artificial nails keep longer nails than keeping natural nails. This result further confirmed that fingernails are a possible reservoir of several microbes and could be implicated in the spread of more virulent microorganisms.},
 year = {2021}
}

Copy | Download

TY  - JOUR
T1  - Isolation and Molecular Characterization of Mycobiota and Other Microbiota from Fingernails
AU  - Adeoti Olatunde Micheal
AU  - Ponle Temitope Elisabeth
AU  - Komolafe Kafilat Adenike
AU  - Adedokun Elisabeth Olajumoke
AU  - Abiola Adebiyi
AU  - Olufemi Samson Olutope
AU  - Adesina David Ademola
Y1  - 2021/03/09
PY  - 2021
N1  - https://doi.org/10.11648/j.ijcda.20210701.11
DO  - 10.11648/j.ijcda.20210701.11
T2  - International Journal of Clinical and Developmental Anatomy
JF  - International Journal of Clinical and Developmental Anatomy
JO  - International Journal of Clinical and Developmental Anatomy
SP  - 1
EP  - 5
PB  - Science Publishing Group
SN  - 2469-8008
UR  - https://doi.org/10.11648/j.ijcda.20210701.11
AB  - This study was carried out to ascertain the hygienic practices among tertiary institutions study and also to isolate and identify microbiota from their thereby conduct molecular screening of fingernails for potential pathogens. A total of 300 nail samples carefully aseptically collected from 30 consented individuals include artificial and natural fingernails from both male and female students of The Oke-Ogun Polytechnic, Saki. The students’ consent were sought for and other ethical issues were complied with as stipulated by the Institutional Ethical Committee. The fingernails were swabbed with sterile swab sticks and thereafter inoculated on the surface of suitably prepared media plates and sub-cultured to obtain pure isolates. Morphological and biochemical tests were carried out on the isolates to confirm the isolates. All students were found to harbour diverse microbes on their undernails. The microorganisms isolated include: E.coli 16.6%, Staphylococcus aureus 22.2%, Streptococcus spp 13.88%, Bacillus spp 25%, members of the Acinetobacter 2.77%, Salmonella spp 13.88%, Fungi 5.55%. The suspected Fungi isolates were sent for sequencing for further identification and characterization. The highest prevalence was found to be more in females than in their male counterparts, which could be as a result of unhygienic practices especially nail-fixing related of artificial nails because those with artificial nails keep longer nails than keeping natural nails. This result further confirmed that fingernails are a possible reservoir of several microbes and could be implicated in the spread of more virulent microorganisms.
VL  - 7
IS  - 1
ER  -

Copy | Download