Background: Direct sputum smear microscopy is the cornerstone for the diagnosis of pulmonary tuberculosis (PTB) in resource-poor countries. However, the requirement for repeated visits to submit specimens and receive results is associated with considerable diagnostic delay, work load, patients drop-out and high expenses for patients. Although the World Health Organization (WHO) has recently changed its policy from spot morning spot (SMS) to spot morning (SM), the SM method still involves two days visits for a patient. Objective: This study evaluated the yield of first spot double slides smears for the diagnosis of PTB in high TB setting. Methods: A total of 362 patients who visited the out-patient department (OPD) of Dilla referral hospital and who were suspected of PTB were involved in the study. In addition SMS sputum samples were collected; double slides smears were prepared, stained by the Ziehl–Neelsen (ZN) method and 100 fields were examined under oil immersion objective for acid fast bacilli (AFB). Results: Of 362, 54(14.92%) were smear-positives. Out of the 54 smear positive subjects, 53 (98.15%) were positives by the first spot specimen. Additionally, 1 of 54 (1.85%) were positives by the morning specimen. Using the 2-day protocol (SMS) among 362 patients, 54(14.92%) were smear positives by double slides and 53(14.64%) by single slide smears. Whereas using the 1-day protocol (first spot); among 362 patients, 53(14.64%) were smear positives both in double slides and single slide smears. Conclusion: The double slides smears from the first spot sputum samples appeared to be as effective as SMS strategy for the diagnosis of PTB though additional studies are required under various settings.
| Published in | International Journal of Immunology (Volume 3, Issue 5) |
| DOI | 10.11648/j.iji.20150305.11 |
| Page(s) | 52-56 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Microscopy, AFB, Sputum, PTB
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APA Style
Basha Ayele Dersie. (2015). The Yield of First Spot Double Slide Smears for the Diagnosis of Pulmonary Tuberculosis. International Journal of Immunology, 3(5), 52-56. https://doi.org/10.11648/j.iji.20150305.11
ACS Style
Basha Ayele Dersie. The Yield of First Spot Double Slide Smears for the Diagnosis of Pulmonary Tuberculosis. Int. J. Immunol. 2015, 3(5), 52-56. doi: 10.11648/j.iji.20150305.11
AMA Style
Basha Ayele Dersie. The Yield of First Spot Double Slide Smears for the Diagnosis of Pulmonary Tuberculosis. Int J Immunol. 2015;3(5):52-56. doi: 10.11648/j.iji.20150305.11
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Download@article{10.11648/j.iji.20150305.11,
author = {Basha Ayele Dersie},
title = {The Yield of First Spot Double Slide Smears for the Diagnosis of Pulmonary Tuberculosis},
journal = {International Journal of Immunology},
volume = {3},
number = {5},
pages = {52-56},
doi = {10.11648/j.iji.20150305.11},
url = {https://doi.org/10.11648/j.iji.20150305.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.iji.20150305.11},
abstract = {Background: Direct sputum smear microscopy is the cornerstone for the diagnosis of pulmonary tuberculosis (PTB) in resource-poor countries. However, the requirement for repeated visits to submit specimens and receive results is associated with considerable diagnostic delay, work load, patients drop-out and high expenses for patients. Although the World Health Organization (WHO) has recently changed its policy from spot morning spot (SMS) to spot morning (SM), the SM method still involves two days visits for a patient. Objective: This study evaluated the yield of first spot double slides smears for the diagnosis of PTB in high TB setting. Methods: A total of 362 patients who visited the out-patient department (OPD) of Dilla referral hospital and who were suspected of PTB were involved in the study. In addition SMS sputum samples were collected; double slides smears were prepared, stained by the Ziehl–Neelsen (ZN) method and 100 fields were examined under oil immersion objective for acid fast bacilli (AFB). Results: Of 362, 54(14.92%) were smear-positives. Out of the 54 smear positive subjects, 53 (98.15%) were positives by the first spot specimen. Additionally, 1 of 54 (1.85%) were positives by the morning specimen. Using the 2-day protocol (SMS) among 362 patients, 54(14.92%) were smear positives by double slides and 53(14.64%) by single slide smears. Whereas using the 1-day protocol (first spot); among 362 patients, 53(14.64%) were smear positives both in double slides and single slide smears. Conclusion: The double slides smears from the first spot sputum samples appeared to be as effective as SMS strategy for the diagnosis of PTB though additional studies are required under various settings.},
year = {2015}
}
TY - JOUR T1 - The Yield of First Spot Double Slide Smears for the Diagnosis of Pulmonary Tuberculosis AU - Basha Ayele Dersie Y1 - 2015/10/16 PY - 2015 N1 - https://doi.org/10.11648/j.iji.20150305.11 DO - 10.11648/j.iji.20150305.11 T2 - International Journal of Immunology JF - International Journal of Immunology JO - International Journal of Immunology SP - 52 EP - 56 PB - Science Publishing Group SN - 2329-1753 UR - https://doi.org/10.11648/j.iji.20150305.11 AB - Background: Direct sputum smear microscopy is the cornerstone for the diagnosis of pulmonary tuberculosis (PTB) in resource-poor countries. However, the requirement for repeated visits to submit specimens and receive results is associated with considerable diagnostic delay, work load, patients drop-out and high expenses for patients. Although the World Health Organization (WHO) has recently changed its policy from spot morning spot (SMS) to spot morning (SM), the SM method still involves two days visits for a patient. Objective: This study evaluated the yield of first spot double slides smears for the diagnosis of PTB in high TB setting. Methods: A total of 362 patients who visited the out-patient department (OPD) of Dilla referral hospital and who were suspected of PTB were involved in the study. In addition SMS sputum samples were collected; double slides smears were prepared, stained by the Ziehl–Neelsen (ZN) method and 100 fields were examined under oil immersion objective for acid fast bacilli (AFB). Results: Of 362, 54(14.92%) were smear-positives. Out of the 54 smear positive subjects, 53 (98.15%) were positives by the first spot specimen. Additionally, 1 of 54 (1.85%) were positives by the morning specimen. Using the 2-day protocol (SMS) among 362 patients, 54(14.92%) were smear positives by double slides and 53(14.64%) by single slide smears. Whereas using the 1-day protocol (first spot); among 362 patients, 53(14.64%) were smear positives both in double slides and single slide smears. Conclusion: The double slides smears from the first spot sputum samples appeared to be as effective as SMS strategy for the diagnosis of PTB though additional studies are required under various settings. VL - 3 IS - 5 ER -
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