Systemic Immunological Responses Among Dental Infection Patients

Zainab Khudher Ahmad Al Mahdi; Fatima Malik Abood

doi:doi:10.11648/j.iji.20210901.12

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Systemic Immunological Responses Among Dental Infection Patients

Zainab Khudher Ahmad Al Mahdi, Fatima Malik Abood

Published in International Journal of Immunology (Volume 9, Issue 1)

Received: 5 December 2020 Accepted: 12 January 2021 Published: 22 January 2021

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Abstract

Dental infections are common health problems, evaluating of some systemic immunological responses are the aim of our study. Our study considered isolation of dental infection bacteria and quantitative evaluation of serum. IgA, IL-4, IL-7 and CD4 and CD8 molecules among dental plaque patients and control group. Oral bacteria from dental infection patients were isolated in appropriate media and diagnosed by biochemical tests and in vitro quantitative determination of serum IgA, IL-4, IL-7 and CD4 and CD8 molecules using ELISA technique. Single and mixed bacterial isolates were noted, mixed infection were (59.25%), the nature of bacteria was Gram positive cocci Streptococcus viridans, Streptococcus pyogenes and Staphylococcus aureus, and Lactocacilli spps, in addition to Gram negative rods black- pigmented bacteria, Klebsilla pneumonia, and Esherishia coli. Serum IgA was higher in patients (368.8±182.5) ng\ml than in control group (319.92±79.26) ng\ml. Serum IL-4 was higher patients (285.33±86.12) pg/ml than in control group (257.7±94.14) pg\ml. Serum IL-7 was higher in control group (19.59±4.14) pg/ml than in dental plaque patients (17.98±3.18) pg /ml. Serum CD4 molecules was higher in control group (1.371±0.5242) ng/ml than in dental plaque patients (1.326±0.1292) ng/ml. Serum CD8 molecules shows non-significant elevation in patients group 0.5825±0.02717 (ng\ml) than in control group 0.51±0.01643 (ng\ml) P≤0.05. Mean of the CD4/CD8 ratio was higher 2.783±1.126 in control group while it was 2.355±0.24 in dental plaque patients, however the differences were non-significant (P≤0.05). The present study conclude that the bacteria isolated from dental infection patients were mixed more than single infection, there were non-significant elevation in IgA, IL-4, and CD8 in patients while IL-7 and CD4 was lower in patients group than in control group, while CD4\CD8 ratio were lower in patients group, these result reflect the fact that mucosal antigen induce systemic tolerance to some extent since these bacteria present in oral cavity in early childhood. Therefor removing these bacteria always the best way to prevent such infections.

Published in	International Journal of Immunology (Volume 9, Issue 1)
DOI	10.11648/j.iji.20210901.12
Page(s)	6-12
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Dental Infection, Immune Response, Cytokine, Oral Bacteria

References

[1]	WHO, World Health Organization. 2019. Oral health. What is the burden of oral disease?. WHO. int.
[2]	Negrini T. D,; Duque C.; Hofling J. F. and Goncalves R. B. (2009). Fundamental mechanisms of immune response to oral bacteria and the main perspectives of a vaccine against dental caries: A brief review. Rev. odonto ciênc. 24 (2): 198-204.
[3]	Samaranayke L. (2012). Essential microbiology for dentistry. 4th ed. Elsevier Ltd. P: 279-285.
[4]	Baucells, B. J.; Mercadal Hally, M.; Álvarez Sánchez, A. T.; Figueras Aloy, J. (2016). Asociaciones de probióticos para la prevención de la enterocolitis necrosante y la reducción de la sepsis tardía y la mortalidad neonatal en recién nacidos pretérmino de menos de 1.500g: una revisión sistemática". Anales de Pediatría. 85 (5): 247-255.
[5]	Wang, H., & Ren, D. (2017). Controlling Streptococcus mutans and Staphylococcus aureus biofilms with direct current and chlorhexidine. AMB Expr 7, 204.
[6]	Caufield P W, Schon C N, Saraithong P and Argimon S. (2015). Oral Lactobacilli and Dental Caries, A Model for Niche Adaptation in Humans. J Dent Res 94 (9 Suppl): 110S–118S.
[7]	Nobbs, A. H., Lamont, R. J., & Jenkinson, H. F. (2009). Streptococcus adherence and colonization Microbiology and molecular biology reviews: MMBR, 73 (3), 407–450.
[8]	Inagaki, Y., Abe, M., Inaki, R., Zong, L., Suenaga, H., Abe, T., & Hoshi, K. (2017). A Case of systemic infection caused by Streptococcus pyogenes oral infection in an edentulous patient. Diseases (Basel, Switzerland). 5 (3), 17.
[9]	Patel M, Chettiar TP, Wadee AA. (2009). Isolation of Staphylococcus aureus and black-pigmented bacteroides indicate a high risk for the development of Ludwig's angina. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 108 (5): 667-72.
[10]	Sundqvist G. (1993). Pathogenicity and virulence of black-pigmented Gram-negative anaerobes. FEMS. Immunology and Medical Microbiology. 1 6: (2-3) 125-138.
[11]	Hornick D., Allen, B. L. Horn and S. Clegg. (1992). Adherence to respiratory epithelia by recombinant Escherichia coli expressing Klebsiella pneumoniae type 3 fimberial gene products. Infect Immun. 60 (4): 1577-88.
[12]	Bao K and Reinhardt R L. (2015). The differential expression of IL-4 and IL-13 and its impact on type-2 immunity. Cytokine. 75 (1): 25-37.
[13]	Luzina I G, Keegan A D, Heller N M, Rook G A W, Donohue T S and Atamas S P. (2012). Regulation of inflammation by interleukin-4: a review of “alternatives”. Format: AbstractSend to J Leukoc Biol. 92 (4): 753-64.
[14]	Lin J, Zhu Z, Xiao H, Wakefield M R, Ding V A, Bal Q and Fang Y. (2017). The role of IL-7 in immunity and cancer. Anticancer Res. 37 (3): 963-967.
[15]	Roitt I. and Rabson A. (2000). Really Essential Medical Immunology. First published, Black well science. London. p: 72.
[16]	Frank L, Nugel E, Docke WD and Porstmann T. (1994). Quantitative determination of CD4/CD8 molecules by a cell marker ELISA. Clin Chem. 40 (1): 38-42.
[17]	Diagbouga S, Durand G, Sanou PT, Dahourou H and Ledru E. (1999). Evaluation of a quantitative determination of CD4 and CD8 molecules as an alternative to CD4 and CD8 T lymphocyte counts in Africans. Tropical Medicine and International Health. 4 (2): 79–84.
[18]	MacFaddin, J. F. (2000). Biochemical test for identification of medical bacteria. 3rd ed., Williams and Wilkins-Baltimor, USA.
[19]	Forbes, B. A.; Daniel, F. S.; and Akice, S. W. (2007). Bailey and Scott's diagnostic microbiology. 12th ed., Mosby Elsevier company, USA.
[20]	Al-Rawi, A. M. (2012). Detection of Porphyromonas gingivalis from Periodontal Pocket Infection by Microbial Cultivation and PCR Techniques. J Rafidain Sci. 23: 39-55.
[21]	Kityamuwesi R., Muwaz L, Kasangaki A, Kajumbula H, and Rwenyonyi C M. 2015. Characteristics of pyogenic odontogenic infection in patients attending Mulago Hospital, Uganda: a cross-sectional study. BMC Microbiol. 15: 46.
[22]	Abranches J, Zeng L, Kajfasz JK, et al. (2018). Biology of Oral Streptococci. Microbiol. 6 (5): 10.1128/m.
[23]	Al-Janabi1 A S, Al-Mahdi Z K and Alhamadi W W. (2019). Fixed Orthodontic Appliance Associated With Change in Bacterial Diversity During First Stage of Active Orthodonotic Treatment. J Global Pharma Technol. 11 (03): 439-448.
[24]	Poveda-Roda R, Jiménez Y, Carbonell E, Gavaldá C, Margaix-Muñoz M, Sarrión Pérez G. (2008) Bacteremia originating in the oral cavity. A review. Med Oral Patol Oral Cir Bucal. 13 (6): E355-62.
[25]	Zhou X, Li Y. (2015) Atlas of oral microbiology, from healthy microflora to disease. 1st Edition. Elsevier Inc. pp: 95.
[26]	Scannapieco FA. (2004). Periodontal inflammation: from gingivitis to systemic disease? Compend Cont Educ Dent 2004; 25 (7) (Suppl 1): 16-25.
[27]	Samaranayake L. (2012). Essential microbiology for dentistry. 4th ed. p 292. Elsevier Ltd, Inc, BV p: 292.
[28]	Leishman S J, Do H L and Ford D J. 2010. Cardiovascular disease and the role of oral bacteria. J Oral Mcrobiol. 2: 5781: 1-13.
[29]	Mariotti A. (2004). A primer on inflammation. Compend Cont Educ Dent. 25 (7) (Suppl 1): 7-15.
[30]	Armitage GC. (2003). Diagnosis of periodontal diseases. J Periodontol. 74; 1237-47.
[31]	Scannapieco FA (2004). Periodontal inflammation: from gingivitis to systemic disease? Compend Cont Educ Dent. 25 (7) (Suppl 1): 16-25.
[32]	Gurenlian J R. (2006). Inflamation: The relationship between oral health and systemic disease. Special supplemental issue. Sponsored by Colgate. P: 1-8.
[33]	Gonzalez-Quintela, A, Alende R, Gude F, Campos J, Rey J, Meijide LM, Fernandez-Merino C and Vidal C. (2008). Serum levels of immunoglobulins (IgG, IgA, IgM) in a general adult population and their relationship with alcohol consumption, smoking and common metabolic abnormalities. Clin Exp Immunol. 151 (1): 42–50.
[34]	Hansen IS, Baeten DLP, den Dunnen J. (2019). The inflammatory function of human IgA. Cell Mol Life Sci. 76: 1041–55.
[35]	Park H-J, Shim HS, An K, Starkweather A, Kim KS and Shim I. (2015). IL-4 Inhibits IL-1𝛽-Induced Depressive-Like Behavior and Central Neurotransmitter Alterations. Mediators of Inflammation: 1-9.
[36]	Shnawa, I M S, Al-Mahdi Z K A. (2015). Oral Mucosal Tolerance Versus Systemic Immune Response to Salmonella typhi Antigen. American J. Biome and Life Sci. Advances in Oral Immunity. 3 (4-1): 13-16.

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APA Style

Zainab Khudher Ahmad Al Mahdi, Fatima Malik Abood. (2021). Systemic Immunological Responses Among Dental Infection Patients. International Journal of Immunology, 9(1), 6-12. https://doi.org/10.11648/j.iji.20210901.12

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Zainab Khudher Ahmad Al Mahdi; Fatima Malik Abood. Systemic Immunological Responses Among Dental Infection Patients. Int. J. Immunol. 2021, 9(1), 6-12. doi: 10.11648/j.iji.20210901.12

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AMA Style

Zainab Khudher Ahmad Al Mahdi, Fatima Malik Abood. Systemic Immunological Responses Among Dental Infection Patients. Int J Immunol. 2021;9(1):6-12. doi: 10.11648/j.iji.20210901.12

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@article{10.11648/j.iji.20210901.12,
  author = {Zainab Khudher Ahmad Al Mahdi and Fatima Malik Abood},
  title = {Systemic Immunological Responses Among Dental Infection Patients},
  journal = {International Journal of Immunology},
  volume = {9},
  number = {1},
  pages = {6-12},
  doi = {10.11648/j.iji.20210901.12},
  url = {https://doi.org/10.11648/j.iji.20210901.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.iji.20210901.12},
  abstract = {Dental infections are common health problems, evaluating of some systemic immunological responses are the aim of our study. Our study considered isolation of dental infection bacteria and quantitative evaluation of serum. IgA, IL-4, IL-7 and CD4 and CD8 molecules among dental plaque patients and control group. Oral bacteria from dental infection patients were isolated in appropriate media and diagnosed by biochemical tests and in vitro quantitative determination of serum IgA, IL-4, IL-7 and CD4 and CD8 molecules using ELISA technique. Single and mixed bacterial isolates were noted, mixed infection were (59.25%), the nature of bacteria was Gram positive cocci Streptococcus viridans, Streptococcus pyogenes and Staphylococcus aureus, and Lactocacilli spps, in addition to Gram negative rods black- pigmented bacteria, Klebsilla pneumonia, and Esherishia coli. Serum IgA was higher in patients (368.8±182.5) ng\ml than in control group (319.92±79.26) ng\ml. Serum IL-4 was higher patients (285.33±86.12) pg/ml than in control group (257.7±94.14) pg\ml. Serum IL-7 was higher in control group (19.59±4.14) pg/ml than in dental plaque patients (17.98±3.18) pg /ml. Serum CD4 molecules was higher in control group (1.371±0.5242) ng/ml than in dental plaque patients (1.326±0.1292) ng/ml. Serum CD8 molecules shows non-significant elevation in patients group 0.5825±0.02717 (ng\ml) than in control group 0.51±0.01643 (ng\ml) P≤0.05. Mean of the CD4/CD8 ratio was higher 2.783±1.126 in control group while it was 2.355±0.24 in dental plaque patients, however the differences were non-significant (P≤0.05). The present study conclude that the bacteria isolated from dental infection patients were mixed more than single infection, there were non-significant elevation in IgA, IL-4, and CD8 in patients while IL-7 and CD4 was lower in patients group than in control group, while CD4\CD8 ratio were lower in patients group, these result reflect the fact that mucosal antigen induce systemic tolerance to some extent since these bacteria present in oral cavity in early childhood. Therefor removing these bacteria always the best way to prevent such infections.},
 year = {2021}
}

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TY  - JOUR
T1  - Systemic Immunological Responses Among Dental Infection Patients
AU  - Zainab Khudher Ahmad Al Mahdi
AU  - Fatima Malik Abood
Y1  - 2021/01/22
PY  - 2021
N1  - https://doi.org/10.11648/j.iji.20210901.12
DO  - 10.11648/j.iji.20210901.12
T2  - International Journal of Immunology
JF  - International Journal of Immunology
JO  - International Journal of Immunology
SP  - 6
EP  - 12
PB  - Science Publishing Group
SN  - 2329-1753
UR  - https://doi.org/10.11648/j.iji.20210901.12
AB  - Dental infections are common health problems, evaluating of some systemic immunological responses are the aim of our study. Our study considered isolation of dental infection bacteria and quantitative evaluation of serum. IgA, IL-4, IL-7 and CD4 and CD8 molecules among dental plaque patients and control group. Oral bacteria from dental infection patients were isolated in appropriate media and diagnosed by biochemical tests and in vitro quantitative determination of serum IgA, IL-4, IL-7 and CD4 and CD8 molecules using ELISA technique. Single and mixed bacterial isolates were noted, mixed infection were (59.25%), the nature of bacteria was Gram positive cocci Streptococcus viridans, Streptococcus pyogenes and Staphylococcus aureus, and Lactocacilli spps, in addition to Gram negative rods black- pigmented bacteria, Klebsilla pneumonia, and Esherishia coli. Serum IgA was higher in patients (368.8±182.5) ng\ml than in control group (319.92±79.26) ng\ml. Serum IL-4 was higher patients (285.33±86.12) pg/ml than in control group (257.7±94.14) pg\ml. Serum IL-7 was higher in control group (19.59±4.14) pg/ml than in dental plaque patients (17.98±3.18) pg /ml. Serum CD4 molecules was higher in control group (1.371±0.5242) ng/ml than in dental plaque patients (1.326±0.1292) ng/ml. Serum CD8 molecules shows non-significant elevation in patients group 0.5825±0.02717 (ng\ml) than in control group 0.51±0.01643 (ng\ml) P≤0.05. Mean of the CD4/CD8 ratio was higher 2.783±1.126 in control group while it was 2.355±0.24 in dental plaque patients, however the differences were non-significant (P≤0.05). The present study conclude that the bacteria isolated from dental infection patients were mixed more than single infection, there were non-significant elevation in IgA, IL-4, and CD8 in patients while IL-7 and CD4 was lower in patients group than in control group, while CD4\CD8 ratio were lower in patients group, these result reflect the fact that mucosal antigen induce systemic tolerance to some extent since these bacteria present in oral cavity in early childhood. Therefor removing these bacteria always the best way to prevent such infections.
VL  - 9
IS  - 1
ER  -

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Author Information

Zainab Khudher Ahmad Al Mahdi

Department of Microbiology, College of Dentistry, University of Babylon, Hilla City, Iraq
Fatima Malik Abood

Department of Microbiology, College of Dentistry, University of Babylon, Hilla City, Iraq

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APA Style

Zainab Khudher Ahmad Al Mahdi, Fatima Malik Abood. (2021). Systemic Immunological Responses Among Dental Infection Patients. International Journal of Immunology, 9(1), 6-12. https://doi.org/10.11648/j.iji.20210901.12

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ACS Style

Zainab Khudher Ahmad Al Mahdi; Fatima Malik Abood. Systemic Immunological Responses Among Dental Infection Patients. Int. J. Immunol. 2021, 9(1), 6-12. doi: 10.11648/j.iji.20210901.12

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AMA Style

Zainab Khudher Ahmad Al Mahdi, Fatima Malik Abood. Systemic Immunological Responses Among Dental Infection Patients. Int J Immunol. 2021;9(1):6-12. doi: 10.11648/j.iji.20210901.12

Copy | Download

@article{10.11648/j.iji.20210901.12,
  author = {Zainab Khudher Ahmad Al Mahdi and Fatima Malik Abood},
  title = {Systemic Immunological Responses Among Dental Infection Patients},
  journal = {International Journal of Immunology},
  volume = {9},
  number = {1},
  pages = {6-12},
  doi = {10.11648/j.iji.20210901.12},
  url = {https://doi.org/10.11648/j.iji.20210901.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.iji.20210901.12},
  abstract = {Dental infections are common health problems, evaluating of some systemic immunological responses are the aim of our study. Our study considered isolation of dental infection bacteria and quantitative evaluation of serum. IgA, IL-4, IL-7 and CD4 and CD8 molecules among dental plaque patients and control group. Oral bacteria from dental infection patients were isolated in appropriate media and diagnosed by biochemical tests and in vitro quantitative determination of serum IgA, IL-4, IL-7 and CD4 and CD8 molecules using ELISA technique. Single and mixed bacterial isolates were noted, mixed infection were (59.25%), the nature of bacteria was Gram positive cocci Streptococcus viridans, Streptococcus pyogenes and Staphylococcus aureus, and Lactocacilli spps, in addition to Gram negative rods black- pigmented bacteria, Klebsilla pneumonia, and Esherishia coli. Serum IgA was higher in patients (368.8±182.5) ng\ml than in control group (319.92±79.26) ng\ml. Serum IL-4 was higher patients (285.33±86.12) pg/ml than in control group (257.7±94.14) pg\ml. Serum IL-7 was higher in control group (19.59±4.14) pg/ml than in dental plaque patients (17.98±3.18) pg /ml. Serum CD4 molecules was higher in control group (1.371±0.5242) ng/ml than in dental plaque patients (1.326±0.1292) ng/ml. Serum CD8 molecules shows non-significant elevation in patients group 0.5825±0.02717 (ng\ml) than in control group 0.51±0.01643 (ng\ml) P≤0.05. Mean of the CD4/CD8 ratio was higher 2.783±1.126 in control group while it was 2.355±0.24 in dental plaque patients, however the differences were non-significant (P≤0.05). The present study conclude that the bacteria isolated from dental infection patients were mixed more than single infection, there were non-significant elevation in IgA, IL-4, and CD8 in patients while IL-7 and CD4 was lower in patients group than in control group, while CD4\CD8 ratio were lower in patients group, these result reflect the fact that mucosal antigen induce systemic tolerance to some extent since these bacteria present in oral cavity in early childhood. Therefor removing these bacteria always the best way to prevent such infections.},
 year = {2021}
}

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TY  - JOUR
T1  - Systemic Immunological Responses Among Dental Infection Patients
AU  - Zainab Khudher Ahmad Al Mahdi
AU  - Fatima Malik Abood
Y1  - 2021/01/22
PY  - 2021
N1  - https://doi.org/10.11648/j.iji.20210901.12
DO  - 10.11648/j.iji.20210901.12
T2  - International Journal of Immunology
JF  - International Journal of Immunology
JO  - International Journal of Immunology
SP  - 6
EP  - 12
PB  - Science Publishing Group
SN  - 2329-1753
UR  - https://doi.org/10.11648/j.iji.20210901.12
AB  - Dental infections are common health problems, evaluating of some systemic immunological responses are the aim of our study. Our study considered isolation of dental infection bacteria and quantitative evaluation of serum. IgA, IL-4, IL-7 and CD4 and CD8 molecules among dental plaque patients and control group. Oral bacteria from dental infection patients were isolated in appropriate media and diagnosed by biochemical tests and in vitro quantitative determination of serum IgA, IL-4, IL-7 and CD4 and CD8 molecules using ELISA technique. Single and mixed bacterial isolates were noted, mixed infection were (59.25%), the nature of bacteria was Gram positive cocci Streptococcus viridans, Streptococcus pyogenes and Staphylococcus aureus, and Lactocacilli spps, in addition to Gram negative rods black- pigmented bacteria, Klebsilla pneumonia, and Esherishia coli. Serum IgA was higher in patients (368.8±182.5) ng\ml than in control group (319.92±79.26) ng\ml. Serum IL-4 was higher patients (285.33±86.12) pg/ml than in control group (257.7±94.14) pg\ml. Serum IL-7 was higher in control group (19.59±4.14) pg/ml than in dental plaque patients (17.98±3.18) pg /ml. Serum CD4 molecules was higher in control group (1.371±0.5242) ng/ml than in dental plaque patients (1.326±0.1292) ng/ml. Serum CD8 molecules shows non-significant elevation in patients group 0.5825±0.02717 (ng\ml) than in control group 0.51±0.01643 (ng\ml) P≤0.05. Mean of the CD4/CD8 ratio was higher 2.783±1.126 in control group while it was 2.355±0.24 in dental plaque patients, however the differences were non-significant (P≤0.05). The present study conclude that the bacteria isolated from dental infection patients were mixed more than single infection, there were non-significant elevation in IgA, IL-4, and CD8 in patients while IL-7 and CD4 was lower in patients group than in control group, while CD4\CD8 ratio were lower in patients group, these result reflect the fact that mucosal antigen induce systemic tolerance to some extent since these bacteria present in oral cavity in early childhood. Therefor removing these bacteria always the best way to prevent such infections.
VL  - 9
IS  - 1
ER  -

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