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Improvement of Antimicrobial and Anti-biofilm Potentials of Mouthwashes by Chitosan Produced by Lactic Acid Bacteria: An in vitro Study

Received: 8 October 2016     Accepted: 14 December 2016     Published: 20 January 2017
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Abstract

Introduction: Modern dentistry emphasizes the importance of dental plaque control to improve oral health. To that end the development of oral care formulations has been geared toward the incorporation of antiplaque agents that may play a crucial role in oral health maintenance. Aims: The aims of this work were to incorporate chitosan produced by Lactobacillus plantarum into a mouthwash matrix and assess its effect upon microbial adherence and biofilm formation of oral microorganisms. Additionally, the action of the chitosan mouthwash was compared with two commercially mouthwashes. Methods: A total of 38 lactic acid bacteria, belonging to Lactobacillus species, isolated from 24 samples of traditional Egyptian dairy products, were screened for chitin degradation. Lactobacillus plantarum is the best producer of the enzyme chitin deacetylase so as to release chitosan. Results: The chitosan containing mouthwashwes capable of interfering with all microorganisms’ growth, adherence and biofilm formation and showing vastly superior activity than both chitosan and commercial mouthwashes assayed. Conclusions: Chitosan mouthwashes show great potential as a natural and efficient alternative to traditional mouthwashes.

Published in International Journal of Microbiology and Biotechnology (Volume 2, Issue 2)
DOI 10.11648/j.ijmb.20170202.11
Page(s) 52-57
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Shrimp Waste, Chitosan, Lactic Acid Bacteria, Anti-adherence, Biofilm Formation

References
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    Eman Zakaria Gomaa. (2017). Improvement of Antimicrobial and Anti-biofilm Potentials of Mouthwashes by Chitosan Produced by Lactic Acid Bacteria: An in vitro Study. International Journal of Microbiology and Biotechnology, 2(2), 52-57. https://doi.org/10.11648/j.ijmb.20170202.11

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    Eman Zakaria Gomaa. Improvement of Antimicrobial and Anti-biofilm Potentials of Mouthwashes by Chitosan Produced by Lactic Acid Bacteria: An in vitro Study. Int. J. Microbiol. Biotechnol. 2017, 2(2), 52-57. doi: 10.11648/j.ijmb.20170202.11

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    AMA Style

    Eman Zakaria Gomaa. Improvement of Antimicrobial and Anti-biofilm Potentials of Mouthwashes by Chitosan Produced by Lactic Acid Bacteria: An in vitro Study. Int J Microbiol Biotechnol. 2017;2(2):52-57. doi: 10.11648/j.ijmb.20170202.11

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  • @article{10.11648/j.ijmb.20170202.11,
      author = {Eman Zakaria Gomaa},
      title = {Improvement of Antimicrobial and Anti-biofilm Potentials of Mouthwashes by Chitosan Produced by Lactic Acid Bacteria: An in vitro Study},
      journal = {International Journal of Microbiology and Biotechnology},
      volume = {2},
      number = {2},
      pages = {52-57},
      doi = {10.11648/j.ijmb.20170202.11},
      url = {https://doi.org/10.11648/j.ijmb.20170202.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20170202.11},
      abstract = {Introduction: Modern dentistry emphasizes the importance of dental plaque control to improve oral health. To that end the development of oral care formulations has been geared toward the incorporation of antiplaque agents that may play a crucial role in oral health maintenance. Aims: The aims of this work were to incorporate chitosan produced by Lactobacillus plantarum into a mouthwash matrix and assess its effect upon microbial adherence and biofilm formation of oral microorganisms. Additionally, the action of the chitosan mouthwash was compared with two commercially mouthwashes. Methods: A total of 38 lactic acid bacteria, belonging to Lactobacillus species, isolated from 24 samples of traditional Egyptian dairy products, were screened for chitin degradation. Lactobacillus plantarum is the best producer of the enzyme chitin deacetylase so as to release chitosan. Results: The chitosan containing mouthwashwes capable of interfering with all microorganisms’ growth, adherence and biofilm formation and showing vastly superior activity than both chitosan and commercial mouthwashes assayed. Conclusions: Chitosan mouthwashes show great potential as a natural and efficient alternative to traditional mouthwashes.},
     year = {2017}
    }
    

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  • TY  - JOUR
    T1  - Improvement of Antimicrobial and Anti-biofilm Potentials of Mouthwashes by Chitosan Produced by Lactic Acid Bacteria: An in vitro Study
    AU  - Eman Zakaria Gomaa
    Y1  - 2017/01/20
    PY  - 2017
    N1  - https://doi.org/10.11648/j.ijmb.20170202.11
    DO  - 10.11648/j.ijmb.20170202.11
    T2  - International Journal of Microbiology and Biotechnology
    JF  - International Journal of Microbiology and Biotechnology
    JO  - International Journal of Microbiology and Biotechnology
    SP  - 52
    EP  - 57
    PB  - Science Publishing Group
    SN  - 2578-9686
    UR  - https://doi.org/10.11648/j.ijmb.20170202.11
    AB  - Introduction: Modern dentistry emphasizes the importance of dental plaque control to improve oral health. To that end the development of oral care formulations has been geared toward the incorporation of antiplaque agents that may play a crucial role in oral health maintenance. Aims: The aims of this work were to incorporate chitosan produced by Lactobacillus plantarum into a mouthwash matrix and assess its effect upon microbial adherence and biofilm formation of oral microorganisms. Additionally, the action of the chitosan mouthwash was compared with two commercially mouthwashes. Methods: A total of 38 lactic acid bacteria, belonging to Lactobacillus species, isolated from 24 samples of traditional Egyptian dairy products, were screened for chitin degradation. Lactobacillus plantarum is the best producer of the enzyme chitin deacetylase so as to release chitosan. Results: The chitosan containing mouthwashwes capable of interfering with all microorganisms’ growth, adherence and biofilm formation and showing vastly superior activity than both chitosan and commercial mouthwashes assayed. Conclusions: Chitosan mouthwashes show great potential as a natural and efficient alternative to traditional mouthwashes.
    VL  - 2
    IS  - 2
    ER  - 

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Author Information
  • Department of Biological and Geological Sciences, Faculty of Education, Ain Shams University, Cairo, Egypt

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