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Evaluation Growth of Tea Seedling and Population of Azotobacter sp. from Application Compost of Green Tea Factory Waste (Tea Fluff) and Azotobacter sp. on Andisols

Received: 7 August 2021    Accepted: 18 August 2021    Published: 26 August 2021
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Abstract

Tea plants are generally propagated through vegetative propagation leaf cutting and planting on mixed of topsoil and subsoil. To obtain the soil media from top soil began to feel difficult. In order to overcome these problems and preserve the environment, it is necessary to find alternative planting medium of tea seedling has to be explored to reduce the use of soil, one of which is the use of green tea factory waste compost Tea Fluff (TF). In addition, the fulfillment of nitrogen with use Azotobacter sp. This study aims to determine the effect of giving Tea Fluff compost and Azotobacter sp. on the growth of tea seedling and soil nutrients. This research was conducted from April 2020 to January 2021. The experiment will be carried out at Nursery in Research Institute for Tea and Chincona (RITC) Gambung, West Java. The planting material used was superior GMB 7 clones. The study used a factorial randomized block design (6 x 2) with 3 replications. Factor I is the combination of subsoil, (F1) 100% Topsoil + 0% TF; (70% Topsoil + 30% TF; 60% Topsoil + 40% TF; 50% Topsoil + 50% TF; 40% Topsoil + 60% TF; and 30% Topsoil + 70% TF, as a factor II is the Azotobcater sp dose, consisting of without Azotobacter sp. and 3 mL Azotobacter sp. The variables observed included the growth of tea seeds (18 WAP), analysis of soil chemistry and the microbial population of Azotobactersp. The results showed that the combination of Tea Fluff compost with soil media had an effect on increasing the number of leaves, stem diameter, root length, root volume, percentage of live seedling, C-organic, N total, dan P avalaible. Azotobacter sp. affect the number of microbial populations. There is an interaction between the combination of Tea Fluff compost with Azotobacter sp. on population of Azotobacter sp. The highest percentage of live seeds in control treatment was 14.83% followed by treatment 70% Topsoil + 30%TF is 14.17%. The composition of the planting medium with Tea Fluff compost planting media affects microbial populations differently and the growth of clones GMB 7 on Andisols.

Published in International Journal of Natural Resource Ecology and Management (Volume 6, Issue 3)
DOI 10.11648/j.ijnrem.20210603.17
Page(s) 156-162
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Azotobacter sp., Tea Seedling, Tea fluff Compost, Andisols

References
[1] Aasfar, A., A. Bargaz., K. Yaakoubi. A, Hilall., I. Bennis., Y. Zeroual., and I. M, Kadmiri., (2021). Nitrogen Fixing Azotobacter Spesies as Potential Soil Biological Enhancers for Crop Nutrition Yield Stability. Frontiers in Microbiology Vol 12. https://doi.org/10.3389/fmicb.2021.628379
[2] Arifin, M. S., dan H. Semangun. (1999). Tea Industry In Indonesia. Global Advances inTea Science. pp. 65 to 82.
[3] Bais, HP., Weir TL, Perry LG., Gilroy S. And JM Vivanco. (2006). The role of root exudates in rhizosphere interaction with plants and other organisms. Annu Rev Plant Biol. 57: 233-66.
[4] Barea, JM., Pozo MJ, Azcon, R and C. Azcon-Aguilar. (2005). Microbial cooperation in the rhizosphere. J. exp. Botany. 56: 417 (1761-1778)
[5] Bhaduri, J., P. Kundu., D. Mitra and S. K, Roy. (2016). Isolation and characterisation of Nitrogen Fixing Bacteria (Azotobacter sp.) from Tea field soil of Dooars and Darjeeling region of North Bengal, India. International Journal of Engineering Science Invention. ISSN (Online): 2319 – 6734, ISSN (Print): 2319 – 6726 www.ijesi.org.Volume 5 Issue 8 August 2016. PP. 46-51.
[6] National Standardization Agency. (2004). Specifications of Compost from Domestic Organic Waste. SNI 19-7030-2004.
[7] Dachlan, A., Zakaria, B., Pairunan, A. K., dan Syam’un, E. (2012). Inoculation of Azotobacter sp. and Agricultural Waste Compost on Growth and Production in Rice Fields. Hasanuddin University. Makasar. Science No. 19 (122-134).
[8] Dalimoenthe, S. L. (2013). The effects of organic planting medium on growth and root formation of tea seedling at early stage of tea nursery. Tea and Chincona Journal Indonesia, 16 (1), 1–11.
[9] Esturk, Y., S. Ercisli., R. Sekban., A. Haznedar, M. Donmez. (2019). The effect of Plant Growth Promoting Rhizobacteria (PGPR) on rooting and root growth of tea (Camellia sinensis var. Sinensis) cuttings. https://www.rombio.eu/rbl3vol13/8.htm.
[10] Hindersah, R., Adityo, B dan P. Suryatmana. (2016). Population Bacteria And Fungi Plant Growth And Tea (Camellia Sinensis L.) On Two Kinds of Growing Media After inoculation Azotobacter. Agrologia, Vol. 5, No. 1, April 2016, Hal. 1-9.
[11] Hernandez, AIG., M. B. S. Fernandez., R. P. Sanchez., M. A. Sanchez and M. R. Morales-Corts. (2021). Compost Tea Induces Growth and Resistance against Rhizoctonia solani and Phytophthora capsici in Pepper. Agronomy, 11, 781. https://doi.org/10.3390/agronomy11040781.
[12] Markakis, E. A.; Fountoulakis, M. S.; Daskalakis, G. C.; Kokkinis, M.; Ligoxigakis, E. K. (2021). The suppressive effect of compost amendments on Fusarium oxysporum f.sp. radicis-cucumerinum in cucumber and Verticillium dahliae in eggplant. Crop Prot. 2016, 79, 70–79.
[13] Paramita, G., D. Indradewa dan S. Waluyo. (2014). Seedling Growth of Seven Tea Clones (Camellia sinensis (L.) Kuntze) PGL by Provision of Ingredients Natural Growth Hormones. Vegetalika Vol. 3 No. 2, 2014: 1 – 12.
[14] Pranoto, E., S. Pratiwi., H. Wachyuni, dan S. Anindita. (2015). Population Distribution Patterns of Azotobacter sp and Organic Matter in Various Grades of Slope of Tea Plantation in PPTK Gambung Highlands. Biospecies Vol. 8 No. 1, Januari 2015, hal. 33-41.
[15] Pranoto, Eko. (2015). The role of Azotobacter sp. and Indigenous Endophytic Bacteria as Nitrogen Sources for The Growth Of GMB 7 Clone Tea Plants. Thesis. Agriculture Faculty, Soil Science Master. Padjadjaran University. Jatinangor.
[16] Santoso, J., R. Suprihatini, W. Widayat, E. Johan, D. J. R. and A. D. (2006). Technical cultural of the plant. Third Edition. Bandung: Research Institute for Tea and Chincona, Gambung.
[17] Tobing, L. W dan M. Sembiring. (2017). Growth Response of Oil Palm Seeds (Elaeis quineensis Jacq) with the Application of Azotobacter and Cocoa Peel Compost Fertilizer in the Main Nursery. Agropimatech Journal Volume 1 (1); 26-31.
[18] Verrillo, M., M. Salzano., V. Cozzolino., R. Spaccini, and A. Piccolo. (2021). Bioactivity and antimicrobial properties of chemically characterized compost teas from different green composts. Waste Management 120 (2021) 98–107.
[19] Wibowo, S, and K. (2003). The use of various comparisons of chincona peel solid waste and sulfur as a mixture of seed media on the growth of tea cuttings (Camellia sinensis (L.) O. Kuntze) Klon GMB 7. In Proceeding National Tea Symposium. Bandung: Research Institute of Tea and Chincona. Bandung.
[20] Wulansari, R dan E, Rezamela. 2020. The Effect pf Tea Fluff Compost on the Growth of Tea Seedlings (Camellia sinensis (L). Kuntze). Journal of Soil and Land Resources Vol 7 No 2, 2020 Hal 341-350. e-ISSN: 2549 9793, doi: 10.21776/ub.jtsl.2020.
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    Restu Wulansari, Anni Yuniarti, Mieke Rochimi Setiawati. (2021). Evaluation Growth of Tea Seedling and Population of Azotobacter sp. from Application Compost of Green Tea Factory Waste (Tea Fluff) and Azotobacter sp. on Andisols. International Journal of Natural Resource Ecology and Management, 6(3), 156-162. https://doi.org/10.11648/j.ijnrem.20210603.17

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    Restu Wulansari; Anni Yuniarti; Mieke Rochimi Setiawati. Evaluation Growth of Tea Seedling and Population of Azotobacter sp. from Application Compost of Green Tea Factory Waste (Tea Fluff) and Azotobacter sp. on Andisols. Int. J. Nat. Resour. Ecol. Manag. 2021, 6(3), 156-162. doi: 10.11648/j.ijnrem.20210603.17

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    AMA Style

    Restu Wulansari, Anni Yuniarti, Mieke Rochimi Setiawati. Evaluation Growth of Tea Seedling and Population of Azotobacter sp. from Application Compost of Green Tea Factory Waste (Tea Fluff) and Azotobacter sp. on Andisols. Int J Nat Resour Ecol Manag. 2021;6(3):156-162. doi: 10.11648/j.ijnrem.20210603.17

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  • @article{10.11648/j.ijnrem.20210603.17,
      author = {Restu Wulansari and Anni Yuniarti and Mieke Rochimi Setiawati},
      title = {Evaluation Growth of Tea Seedling and Population of Azotobacter sp. from Application Compost of Green Tea Factory Waste (Tea Fluff) and Azotobacter sp. on Andisols},
      journal = {International Journal of Natural Resource Ecology and Management},
      volume = {6},
      number = {3},
      pages = {156-162},
      doi = {10.11648/j.ijnrem.20210603.17},
      url = {https://doi.org/10.11648/j.ijnrem.20210603.17},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijnrem.20210603.17},
      abstract = {Tea plants are generally propagated through vegetative propagation leaf cutting and planting on mixed of topsoil and subsoil. To obtain the soil media from top soil began to feel difficult. In order to overcome these problems and preserve the environment, it is necessary to find alternative planting medium of tea seedling has to be explored to reduce the use of soil, one of which is the use of green tea factory waste compost Tea Fluff (TF). In addition, the fulfillment of nitrogen with use Azotobacter sp. This study aims to determine the effect of giving Tea Fluff compost and Azotobacter sp. on the growth of tea seedling and soil nutrients. This research was conducted from April 2020 to January 2021. The experiment will be carried out at Nursery in Research Institute for Tea and Chincona (RITC) Gambung, West Java. The planting material used was superior GMB 7 clones. The study used a factorial randomized block design (6 x 2) with 3 replications. Factor I is the combination of subsoil, (F1) 100% Topsoil + 0% TF; (70% Topsoil + 30% TF; 60% Topsoil + 40% TF; 50% Topsoil + 50% TF; 40% Topsoil + 60% TF; and 30% Topsoil + 70% TF, as a factor II is the Azotobcater sp dose, consisting of without Azotobacter sp. and 3 mL Azotobacter sp. The variables observed included the growth of tea seeds (18 WAP), analysis of soil chemistry and the microbial population of Azotobactersp. The results showed that the combination of Tea Fluff compost with soil media had an effect on increasing the number of leaves, stem diameter, root length, root volume, percentage of live seedling, C-organic, N total, dan P avalaible. Azotobacter sp. affect the number of microbial populations. There is an interaction between the combination of Tea Fluff compost with Azotobacter sp. on population of Azotobacter sp. The highest percentage of live seeds in control treatment was 14.83% followed by treatment 70% Topsoil + 30%TF is 14.17%. The composition of the planting medium with Tea Fluff compost planting media affects microbial populations differently and the growth of clones GMB 7 on Andisols.},
     year = {2021}
    }
    

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  • TY  - JOUR
    T1  - Evaluation Growth of Tea Seedling and Population of Azotobacter sp. from Application Compost of Green Tea Factory Waste (Tea Fluff) and Azotobacter sp. on Andisols
    AU  - Restu Wulansari
    AU  - Anni Yuniarti
    AU  - Mieke Rochimi Setiawati
    Y1  - 2021/08/26
    PY  - 2021
    N1  - https://doi.org/10.11648/j.ijnrem.20210603.17
    DO  - 10.11648/j.ijnrem.20210603.17
    T2  - International Journal of Natural Resource Ecology and Management
    JF  - International Journal of Natural Resource Ecology and Management
    JO  - International Journal of Natural Resource Ecology and Management
    SP  - 156
    EP  - 162
    PB  - Science Publishing Group
    SN  - 2575-3061
    UR  - https://doi.org/10.11648/j.ijnrem.20210603.17
    AB  - Tea plants are generally propagated through vegetative propagation leaf cutting and planting on mixed of topsoil and subsoil. To obtain the soil media from top soil began to feel difficult. In order to overcome these problems and preserve the environment, it is necessary to find alternative planting medium of tea seedling has to be explored to reduce the use of soil, one of which is the use of green tea factory waste compost Tea Fluff (TF). In addition, the fulfillment of nitrogen with use Azotobacter sp. This study aims to determine the effect of giving Tea Fluff compost and Azotobacter sp. on the growth of tea seedling and soil nutrients. This research was conducted from April 2020 to January 2021. The experiment will be carried out at Nursery in Research Institute for Tea and Chincona (RITC) Gambung, West Java. The planting material used was superior GMB 7 clones. The study used a factorial randomized block design (6 x 2) with 3 replications. Factor I is the combination of subsoil, (F1) 100% Topsoil + 0% TF; (70% Topsoil + 30% TF; 60% Topsoil + 40% TF; 50% Topsoil + 50% TF; 40% Topsoil + 60% TF; and 30% Topsoil + 70% TF, as a factor II is the Azotobcater sp dose, consisting of without Azotobacter sp. and 3 mL Azotobacter sp. The variables observed included the growth of tea seeds (18 WAP), analysis of soil chemistry and the microbial population of Azotobactersp. The results showed that the combination of Tea Fluff compost with soil media had an effect on increasing the number of leaves, stem diameter, root length, root volume, percentage of live seedling, C-organic, N total, dan P avalaible. Azotobacter sp. affect the number of microbial populations. There is an interaction between the combination of Tea Fluff compost with Azotobacter sp. on population of Azotobacter sp. The highest percentage of live seeds in control treatment was 14.83% followed by treatment 70% Topsoil + 30%TF is 14.17%. The composition of the planting medium with Tea Fluff compost planting media affects microbial populations differently and the growth of clones GMB 7 on Andisols.
    VL  - 6
    IS  - 3
    ER  - 

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Author Information
  • Soil and Plant Nutrition Division, Research Institute for Tea and Chincona, West Java, Indonesia

  • Department of Soil Science, Faculty of Agriculture, Padjadjaran University, Bandung, Indonesia

  • Department of Soil Science, Faculty of Agriculture, Padjadjaran University, Bandung, Indonesia

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