Antimicrobial Multi-Resistance of Acinetobacter baumanii Isolated from Clinical Specimens in Douala (Cameroon)

Cecile Okalla Ebongue; Emmanuel Roddy Mengue; Jean-Pierre Nda Mefo’o; Martial Dongmo Tsiazok; Raymond N’guessan Kouassi; Elisabeth Ngo Bum

doi:doi:10.11648/j.jdmp.20150102.12

Antimicrobial Multi-Resistance of Acinetobacter baumanii Isolated from Clinical Specimens in Douala (Cameroon)

Cecile Okalla Ebongue, Emmanuel Roddy Mengue, Jean-Pierre Nda Mefo’o, Martial Dongmo Tsiazok, Raymond N’guessan Kouassi, Elisabeth Ngo Bum

Published in Journal of Diseases and Medicinal Plants (Volume 1, Issue 2)

Received: 22 May 2015 Accepted: 31 May 2015 Published: 1 June 2015

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Abstract

Bacteria of the genus Acinetobacter are non-fermentative gram-negative bacilli, often considered as ubiquitous and frequently found in the environment. This work aimed to study the susceptibility of Acinetobacter baumannii isolated at the General Hospital of Douala. This bacterium, which is greatly involved in human pathology, demonstrated high innate and acquired resistance to multiple antibiotics classes. This was a descriptive and retrospective study over a period of ten years (2005-2014), covering all the isolates of Acinetobacter baumannii from in and out patients. Samples were analyzed in the bacteriology laboratory at the General Hospital of Douala. Data collection was made by extraction of the results of all susceptibility of Acinetobacter baumannii, carried out from 2005 to 2014, registered in the memory of the mini API™ system; this automated machine identifies and measures the susceptibility of all the bacterial strains. A total of 266 strains were isolated from suppurations, urine, urinary catheters, and all other specimens received by the laboratory during the study period. These strains obtained from samples collected from all services of the hospital and ambulatory patients. The resistance rates noted were: 67.29 % for ticarcillin, 64.28 % ticarcillin + clavulanic acid, 68.33 % for piperacillin, 56.97 piperacillin + tazobactam, 58.27 % ceftazidime, 58.01 % cefepime, 21.05 % imipenem, 33.46 % colistin, 52.63 % gentamicin, 48.49 % tobramycin, 24.43 % amikacin, 53.40 % netilmicin, 50% for ciprofloxacin and levofloxacin, 51.05 % ofloxacin. The prevalence of multi-resistant strains was 43.18 %, mostly seen in patients hospitalized in the Burns unit. Only imipenem and Amikacin showed good activity on these strains with 78.95 % and 75.57 % of susceptibility respectively. This study showed the low activity of penicillin even in combination with beta lactamases inhibitors, as well as the cephalosporins on strains of Acinetobacter baumannii. Therapeutic support of infections due to this pathogen is highly problematic in General hospital of Douala. However, imipenem and amikacin, and to a lesser extent colistin remains effective treatment choices.

Published in	Journal of Diseases and Medicinal Plants (Volume 1, Issue 2)
DOI	10.11648/j.jdmp.20150102.12
Page(s)	31-36
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Acinetobacter baumannii, Multi-Drug Resistance, Antibiotics, Cameroon

References

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Cecile Okalla Ebongue, Emmanuel Roddy Mengue, Jean-Pierre Nda Mefo’o, Martial Dongmo Tsiazok, Raymond N’guessan Kouassi, et al. (2015). Antimicrobial Multi-Resistance of Acinetobacter baumanii Isolated from Clinical Specimens in Douala (Cameroon). Journal of Diseases and Medicinal Plants, 1(2), 31-36. https://doi.org/10.11648/j.jdmp.20150102.12

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Cecile Okalla Ebongue; Emmanuel Roddy Mengue; Jean-Pierre Nda Mefo’o; Martial Dongmo Tsiazok; Raymond N’guessan Kouassi, et al. Antimicrobial Multi-Resistance of Acinetobacter baumanii Isolated from Clinical Specimens in Douala (Cameroon). J. Dis. Med. Plants 2015, 1(2), 31-36. doi: 10.11648/j.jdmp.20150102.12

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Cecile Okalla Ebongue, Emmanuel Roddy Mengue, Jean-Pierre Nda Mefo’o, Martial Dongmo Tsiazok, Raymond N’guessan Kouassi, et al. Antimicrobial Multi-Resistance of Acinetobacter baumanii Isolated from Clinical Specimens in Douala (Cameroon). J Dis Med Plants. 2015;1(2):31-36. doi: 10.11648/j.jdmp.20150102.12

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@article{10.11648/j.jdmp.20150102.12,
  author = {Cecile Okalla Ebongue and Emmanuel Roddy Mengue and Jean-Pierre Nda Mefo’o and Martial Dongmo Tsiazok and Raymond N’guessan Kouassi and Elisabeth Ngo Bum},
  title = {Antimicrobial Multi-Resistance of Acinetobacter baumanii Isolated from Clinical Specimens in Douala (Cameroon)},
  journal = {Journal of Diseases and Medicinal Plants},
  volume = {1},
  number = {2},
  pages = {31-36},
  doi = {10.11648/j.jdmp.20150102.12},
  url = {https://doi.org/10.11648/j.jdmp.20150102.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20150102.12},
  abstract = {Bacteria of the genus Acinetobacter are non-fermentative gram-negative bacilli, often considered as ubiquitous and frequently found in the environment. This work aimed to study the susceptibility of Acinetobacter baumannii isolated at the General Hospital of Douala. This bacterium, which is greatly involved in human pathology, demonstrated high innate and acquired resistance to multiple antibiotics classes. This was a descriptive and retrospective study over a period of ten years (2005-2014), covering all the isolates of Acinetobacter baumannii from in and out patients. Samples were analyzed in the bacteriology laboratory at the General Hospital of Douala. Data collection was made by extraction of the results of all susceptibility of Acinetobacter baumannii, carried out from 2005 to 2014, registered in the memory of the mini API™ system; this automated machine identifies and measures the susceptibility of all the bacterial strains. A total of 266 strains were isolated from suppurations, urine, urinary catheters, and all other specimens received by the laboratory during the study period. These strains obtained from samples collected from all services of the hospital and ambulatory patients. The resistance rates noted were: 67.29 % for ticarcillin, 64.28 % ticarcillin + clavulanic acid, 68.33 % for piperacillin, 56.97 piperacillin + tazobactam, 58.27 % ceftazidime, 58.01 % cefepime, 21.05 % imipenem, 33.46 % colistin, 52.63 % gentamicin, 48.49 % tobramycin, 24.43 % amikacin, 53.40 % netilmicin, 50% for ciprofloxacin and levofloxacin, 51.05 % ofloxacin. The prevalence of multi-resistant strains was 43.18 %, mostly seen in patients hospitalized in the Burns unit. Only imipenem and Amikacin showed good activity on these strains with 78.95 % and 75.57 % of susceptibility respectively. This study showed the low activity of penicillin even in combination with beta lactamases inhibitors, as well as the cephalosporins on strains of Acinetobacter baumannii. Therapeutic support of infections due to this pathogen is highly problematic in General hospital of Douala. However, imipenem and amikacin, and to a lesser extent colistin remains effective treatment choices.},
 year = {2015}
}

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TY  - JOUR
T1  - Antimicrobial Multi-Resistance of Acinetobacter baumanii Isolated from Clinical Specimens in Douala (Cameroon)
AU  - Cecile Okalla Ebongue
AU  - Emmanuel Roddy Mengue
AU  - Jean-Pierre Nda Mefo’o
AU  - Martial Dongmo Tsiazok
AU  - Raymond N’guessan Kouassi
AU  - Elisabeth Ngo Bum
Y1  - 2015/06/01
PY  - 2015
N1  - https://doi.org/10.11648/j.jdmp.20150102.12
DO  - 10.11648/j.jdmp.20150102.12
T2  - Journal of Diseases and Medicinal Plants
JF  - Journal of Diseases and Medicinal Plants
JO  - Journal of Diseases and Medicinal Plants
SP  - 31
EP  - 36
PB  - Science Publishing Group
SN  - 2469-8210
UR  - https://doi.org/10.11648/j.jdmp.20150102.12
AB  - Bacteria of the genus Acinetobacter are non-fermentative gram-negative bacilli, often considered as ubiquitous and frequently found in the environment. This work aimed to study the susceptibility of Acinetobacter baumannii isolated at the General Hospital of Douala. This bacterium, which is greatly involved in human pathology, demonstrated high innate and acquired resistance to multiple antibiotics classes. This was a descriptive and retrospective study over a period of ten years (2005-2014), covering all the isolates of Acinetobacter baumannii from in and out patients. Samples were analyzed in the bacteriology laboratory at the General Hospital of Douala. Data collection was made by extraction of the results of all susceptibility of Acinetobacter baumannii, carried out from 2005 to 2014, registered in the memory of the mini API™ system; this automated machine identifies and measures the susceptibility of all the bacterial strains. A total of 266 strains were isolated from suppurations, urine, urinary catheters, and all other specimens received by the laboratory during the study period. These strains obtained from samples collected from all services of the hospital and ambulatory patients. The resistance rates noted were: 67.29 % for ticarcillin, 64.28 % ticarcillin + clavulanic acid, 68.33 % for piperacillin, 56.97 piperacillin + tazobactam, 58.27 % ceftazidime, 58.01 % cefepime, 21.05 % imipenem, 33.46 % colistin, 52.63 % gentamicin, 48.49 % tobramycin, 24.43 % amikacin, 53.40 % netilmicin, 50% for ciprofloxacin and levofloxacin, 51.05 % ofloxacin. The prevalence of multi-resistant strains was 43.18 %, mostly seen in patients hospitalized in the Burns unit. Only imipenem and Amikacin showed good activity on these strains with 78.95 % and 75.57 % of susceptibility respectively. This study showed the low activity of penicillin even in combination with beta lactamases inhibitors, as well as the cephalosporins on strains of Acinetobacter baumannii. Therapeutic support of infections due to this pathogen is highly problematic in General hospital of Douala. However, imipenem and amikacin, and to a lesser extent colistin remains effective treatment choices.
VL  - 1
IS  - 2
ER  -

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Author Information

Cecile Okalla Ebongue

Clinical biology Laboratory, General Hospital of Douala, Douala, Cameroon
Emmanuel Roddy Mengue

Clinical biology Laboratory, General Hospital of Douala, Douala, Cameroon
Jean-Pierre Nda Mefo’o

Clinical biology Laboratory, General Hospital of Douala, Douala, Cameroon
Martial Dongmo Tsiazok

Department of Biological Sciences, Faculty of Medicine, University of Douala, Douala, Cameroon
Raymond N’guessan Kouassi

Department of Biological Sciences, Faculty of Medicine, University of Cocody, Abidjan, Ivory Cost
Elisabeth Ngo Bum

Department of Biological Sciences, Faculty of Science, University of Ngaoundere, Ngaoundere, Cameroon

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Cecile Okalla Ebongue, Emmanuel Roddy Mengue, Jean-Pierre Nda Mefo’o, Martial Dongmo Tsiazok, Raymond N’guessan Kouassi, et al. (2015). Antimicrobial Multi-Resistance of Acinetobacter baumanii Isolated from Clinical Specimens in Douala (Cameroon). Journal of Diseases and Medicinal Plants, 1(2), 31-36. https://doi.org/10.11648/j.jdmp.20150102.12

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ACS Style

Cecile Okalla Ebongue; Emmanuel Roddy Mengue; Jean-Pierre Nda Mefo’o; Martial Dongmo Tsiazok; Raymond N’guessan Kouassi, et al. Antimicrobial Multi-Resistance of Acinetobacter baumanii Isolated from Clinical Specimens in Douala (Cameroon). J. Dis. Med. Plants 2015, 1(2), 31-36. doi: 10.11648/j.jdmp.20150102.12

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AMA Style

Cecile Okalla Ebongue, Emmanuel Roddy Mengue, Jean-Pierre Nda Mefo’o, Martial Dongmo Tsiazok, Raymond N’guessan Kouassi, et al. Antimicrobial Multi-Resistance of Acinetobacter baumanii Isolated from Clinical Specimens in Douala (Cameroon). J Dis Med Plants. 2015;1(2):31-36. doi: 10.11648/j.jdmp.20150102.12

Copy | Download

@article{10.11648/j.jdmp.20150102.12,
  author = {Cecile Okalla Ebongue and Emmanuel Roddy Mengue and Jean-Pierre Nda Mefo’o and Martial Dongmo Tsiazok and Raymond N’guessan Kouassi and Elisabeth Ngo Bum},
  title = {Antimicrobial Multi-Resistance of Acinetobacter baumanii Isolated from Clinical Specimens in Douala (Cameroon)},
  journal = {Journal of Diseases and Medicinal Plants},
  volume = {1},
  number = {2},
  pages = {31-36},
  doi = {10.11648/j.jdmp.20150102.12},
  url = {https://doi.org/10.11648/j.jdmp.20150102.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20150102.12},
  abstract = {Bacteria of the genus Acinetobacter are non-fermentative gram-negative bacilli, often considered as ubiquitous and frequently found in the environment. This work aimed to study the susceptibility of Acinetobacter baumannii isolated at the General Hospital of Douala. This bacterium, which is greatly involved in human pathology, demonstrated high innate and acquired resistance to multiple antibiotics classes. This was a descriptive and retrospective study over a period of ten years (2005-2014), covering all the isolates of Acinetobacter baumannii from in and out patients. Samples were analyzed in the bacteriology laboratory at the General Hospital of Douala. Data collection was made by extraction of the results of all susceptibility of Acinetobacter baumannii, carried out from 2005 to 2014, registered in the memory of the mini API™ system; this automated machine identifies and measures the susceptibility of all the bacterial strains. A total of 266 strains were isolated from suppurations, urine, urinary catheters, and all other specimens received by the laboratory during the study period. These strains obtained from samples collected from all services of the hospital and ambulatory patients. The resistance rates noted were: 67.29 % for ticarcillin, 64.28 % ticarcillin + clavulanic acid, 68.33 % for piperacillin, 56.97 piperacillin + tazobactam, 58.27 % ceftazidime, 58.01 % cefepime, 21.05 % imipenem, 33.46 % colistin, 52.63 % gentamicin, 48.49 % tobramycin, 24.43 % amikacin, 53.40 % netilmicin, 50% for ciprofloxacin and levofloxacin, 51.05 % ofloxacin. The prevalence of multi-resistant strains was 43.18 %, mostly seen in patients hospitalized in the Burns unit. Only imipenem and Amikacin showed good activity on these strains with 78.95 % and 75.57 % of susceptibility respectively. This study showed the low activity of penicillin even in combination with beta lactamases inhibitors, as well as the cephalosporins on strains of Acinetobacter baumannii. Therapeutic support of infections due to this pathogen is highly problematic in General hospital of Douala. However, imipenem and amikacin, and to a lesser extent colistin remains effective treatment choices.},
 year = {2015}
}

Copy | Download

TY  - JOUR
T1  - Antimicrobial Multi-Resistance of Acinetobacter baumanii Isolated from Clinical Specimens in Douala (Cameroon)
AU  - Cecile Okalla Ebongue
AU  - Emmanuel Roddy Mengue
AU  - Jean-Pierre Nda Mefo’o
AU  - Martial Dongmo Tsiazok
AU  - Raymond N’guessan Kouassi
AU  - Elisabeth Ngo Bum
Y1  - 2015/06/01
PY  - 2015
N1  - https://doi.org/10.11648/j.jdmp.20150102.12
DO  - 10.11648/j.jdmp.20150102.12
T2  - Journal of Diseases and Medicinal Plants
JF  - Journal of Diseases and Medicinal Plants
JO  - Journal of Diseases and Medicinal Plants
SP  - 31
EP  - 36
PB  - Science Publishing Group
SN  - 2469-8210
UR  - https://doi.org/10.11648/j.jdmp.20150102.12
AB  - Bacteria of the genus Acinetobacter are non-fermentative gram-negative bacilli, often considered as ubiquitous and frequently found in the environment. This work aimed to study the susceptibility of Acinetobacter baumannii isolated at the General Hospital of Douala. This bacterium, which is greatly involved in human pathology, demonstrated high innate and acquired resistance to multiple antibiotics classes. This was a descriptive and retrospective study over a period of ten years (2005-2014), covering all the isolates of Acinetobacter baumannii from in and out patients. Samples were analyzed in the bacteriology laboratory at the General Hospital of Douala. Data collection was made by extraction of the results of all susceptibility of Acinetobacter baumannii, carried out from 2005 to 2014, registered in the memory of the mini API™ system; this automated machine identifies and measures the susceptibility of all the bacterial strains. A total of 266 strains were isolated from suppurations, urine, urinary catheters, and all other specimens received by the laboratory during the study period. These strains obtained from samples collected from all services of the hospital and ambulatory patients. The resistance rates noted were: 67.29 % for ticarcillin, 64.28 % ticarcillin + clavulanic acid, 68.33 % for piperacillin, 56.97 piperacillin + tazobactam, 58.27 % ceftazidime, 58.01 % cefepime, 21.05 % imipenem, 33.46 % colistin, 52.63 % gentamicin, 48.49 % tobramycin, 24.43 % amikacin, 53.40 % netilmicin, 50% for ciprofloxacin and levofloxacin, 51.05 % ofloxacin. The prevalence of multi-resistant strains was 43.18 %, mostly seen in patients hospitalized in the Burns unit. Only imipenem and Amikacin showed good activity on these strains with 78.95 % and 75.57 % of susceptibility respectively. This study showed the low activity of penicillin even in combination with beta lactamases inhibitors, as well as the cephalosporins on strains of Acinetobacter baumannii. Therapeutic support of infections due to this pathogen is highly problematic in General hospital of Douala. However, imipenem and amikacin, and to a lesser extent colistin remains effective treatment choices.
VL  - 1
IS  - 2
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