<i>In Vitro antisickling</i> and Free Radical Scavenging Activities of <i>Kigelia africana</i> (LAM) Stem Bark

Tatiana Kangah Mireille Kple; Joel Akakpo-Akue; Koffi Adou Mathieu Kra; N’Guessan Bra Yvette Fofie; Koffi Julien Golly; Ibrahime Sanogo; Antoinette Claire Chiaye Yapo-Crezoit

doi:doi:10.11648/j.jdmp.20210701.11

In Vitro antisickling and Free Radical Scavenging Activities of Kigelia africana (LAM) Stem Bark

Tatiana Kangah Mireille Kple, Joel Akakpo-Akue, Koffi Adou Mathieu Kra, N’Guessan Bra Yvette Fofie, Koffi Julien Golly, Ibrahime Sanogo, Antoinette Claire Chiaye Yapo-Crezoit

Published in Journal of Diseases and Medicinal Plants (Volume 7, Issue 1)

Received: 12 December 2020 Accepted: 30 December 2020 Published: 30 January 2021

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Abstract

Kigelia africana dried bark has been recommended in the management of sickle cell anemia by traditional practitioners. The aim of this study was to evaluate in vitro antioxidant and sickling inhibitory activities of Kigelia africana. Quantitative estimation of phenolic compounds was performed using colorimetric method in hydro-ethanolic extract (70%) and decocted. The sickling inhibitory activity was determinate according to Emmel method and the evaluation of antioxidant properties was carried out using the method of scavenging the free radical DPPH. The obtained results indicated that Kigelia africana species contains flavonoids (8.61±1.08 mg QE and 9.78±1.14 mg QE/g of dry weight) and total phenols (9.48±0.19b mg GAE/g and 11.11±0.22 mg GAE /g of dry weight) in decocted and hydroethanolic extract respectively. The IC₅₀ values of the ethanolic extract and the decocted were 0.320±0.01 and 0.468±0.04 mg/mL respectively. The in vitro sickle-formation inhibition test indicated the value of 89% and 82.36% for the ethanolic extract and the decocted at 10mg/mL respectively. Those values were higher than 80% which was the phenylalanine value. Both extracts showed antioxidant and sickling inhibitory activities. Overall, there could have a correlation between these activities and phenolic compound content in this studied plant extracts. These results would justify the use of this plant in rural environment.

Published in	Journal of Diseases and Medicinal Plants (Volume 7, Issue 1)
DOI	10.11648/j.jdmp.20210701.11
Page(s)	1-5
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Kigelia africana, Phenolic Compounds, Antisickling Activity, Antioxidant Activity, Cote d’Ivoire

References

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APA Style

Tatiana Kangah Mireille Kple, Joel Akakpo-Akue, Koffi Adou Mathieu Kra, N’Guessan Bra Yvette Fofie, Koffi Julien Golly, et al. (2021). In Vitro antisickling and Free Radical Scavenging Activities of Kigelia africana (LAM) Stem Bark. Journal of Diseases and Medicinal Plants, 7(1), 1-5. https://doi.org/10.11648/j.jdmp.20210701.11

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Tatiana Kangah Mireille Kple; Joel Akakpo-Akue; Koffi Adou Mathieu Kra; N’Guessan Bra Yvette Fofie; Koffi Julien Golly, et al. In Vitro antisickling and Free Radical Scavenging Activities of Kigelia africana (LAM) Stem Bark. J. Dis. Med. Plants 2021, 7(1), 1-5. doi: 10.11648/j.jdmp.20210701.11

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AMA Style

Tatiana Kangah Mireille Kple, Joel Akakpo-Akue, Koffi Adou Mathieu Kra, N’Guessan Bra Yvette Fofie, Koffi Julien Golly, et al. In Vitro antisickling and Free Radical Scavenging Activities of Kigelia africana (LAM) Stem Bark. J Dis Med Plants. 2021;7(1):1-5. doi: 10.11648/j.jdmp.20210701.11

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@article{10.11648/j.jdmp.20210701.11,
  author = {Tatiana Kangah Mireille Kple and Joel Akakpo-Akue and Koffi Adou Mathieu Kra and N’Guessan Bra Yvette Fofie and Koffi Julien Golly and Ibrahime Sanogo and Antoinette Claire Chiaye Yapo-Crezoit},
  title = {In Vitro antisickling and Free Radical Scavenging Activities of Kigelia africana (LAM) Stem Bark},
  journal = {Journal of Diseases and Medicinal Plants},
  volume = {7},
  number = {1},
  pages = {1-5},
  doi = {10.11648/j.jdmp.20210701.11},
  url = {https://doi.org/10.11648/j.jdmp.20210701.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20210701.11},
  abstract = {Kigelia africana dried bark has been recommended in the management of sickle cell anemia by traditional practitioners. The aim of this study was to evaluate in vitro antioxidant and sickling inhibitory activities of Kigelia africana. Quantitative estimation of phenolic compounds was performed using colorimetric method in hydro-ethanolic extract (70%) and decocted. The sickling inhibitory activity was determinate according to Emmel method and the evaluation of antioxidant properties was carried out using the method of scavenging the free radical DPPH. The obtained results indicated that Kigelia africana species contains flavonoids (8.61±1.08 mg QE and 9.78±1.14 mg QE/g of dry weight) and total phenols (9.48±0.19b mg GAE/g and 11.11±0.22 mg GAE /g of dry weight) in decocted and hydroethanolic extract respectively. The IC50 values of the ethanolic extract and the decocted were 0.320±0.01 and 0.468±0.04 mg/mL respectively. The in vitro sickle-formation inhibition test indicated the value of 89% and 82.36% for the ethanolic extract and the decocted at 10mg/mL respectively. Those values were higher than 80% which was the phenylalanine value. Both extracts showed antioxidant and sickling inhibitory activities. Overall, there could have a correlation between these activities and phenolic compound content in this studied plant extracts. These results would justify the use of this plant in rural environment.},
 year = {2021}
}

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TY - JOUR
T1 - In Vitro antisickling and Free Radical Scavenging Activities of Kigelia africana (LAM) Stem Bark
AU - Tatiana Kangah Mireille Kple
AU - Joel Akakpo-Akue
AU - Koffi Adou Mathieu Kra
AU - N’Guessan Bra Yvette Fofie
AU - Koffi Julien Golly
AU - Ibrahime Sanogo
AU - Antoinette Claire Chiaye Yapo-Crezoit
Y1 - 2021/01/30
PY - 2021
N1 - https://doi.org/10.11648/j.jdmp.20210701.11
DO - 10.11648/j.jdmp.20210701.11
T2 - Journal of Diseases and Medicinal Plants
JF - Journal of Diseases and Medicinal Plants
JO - Journal of Diseases and Medicinal Plants
SP - 1
EP - 5
PB - Science Publishing Group
SN - 2469-8210
UR - https://doi.org/10.11648/j.jdmp.20210701.11
AB - Kigelia africana dried bark has been recommended in the management of sickle cell anemia by traditional practitioners. The aim of this study was to evaluate in vitro antioxidant and sickling inhibitory activities of Kigelia africana. Quantitative estimation of phenolic compounds was performed using colorimetric method in hydro-ethanolic extract (70%) and decocted. The sickling inhibitory activity was determinate according to Emmel method and the evaluation of antioxidant properties was carried out using the method of scavenging the free radical DPPH. The obtained results indicated that Kigelia africana species contains flavonoids (8.61±1.08 mg QE and 9.78±1.14 mg QE/g of dry weight) and total phenols (9.48±0.19b mg GAE/g and 11.11±0.22 mg GAE /g of dry weight) in decocted and hydroethanolic extract respectively. The IC50 values of the ethanolic extract and the decocted were 0.320±0.01 and 0.468±0.04 mg/mL respectively. The in vitro sickle-formation inhibition test indicated the value of 89% and 82.36% for the ethanolic extract and the decocted at 10mg/mL respectively. Those values were higher than 80% which was the phenylalanine value. Both extracts showed antioxidant and sickling inhibitory activities. Overall, there could have a correlation between these activities and phenolic compound content in this studied plant extracts. These results would justify the use of this plant in rural environment.
VL - 7
IS - 1
ER -

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Author Information

Tatiana Kangah Mireille Kple

Laboratory of Biology and Health, University Felix Houphouet-Boigny (UFHB), Abidjan, Cote d’Ivoire
Joel Akakpo-Akue

Laboratory of Biology and Health, University Felix Houphouet-Boigny (UFHB), Abidjan, Cote d’Ivoire
Koffi Adou Mathieu Kra

Laboratory of Biology and Health, University Felix Houphouet-Boigny (UFHB), Abidjan, Cote d’Ivoire
N’Guessan Bra Yvette Fofie

Pharmacognosy Laboratory, University Felix Houphouet-Boigny, Abidjan, Cote d’Ivoire
Koffi Julien Golly

Immunity Biology Center, Pasteur Institute of Cote d’Ivoire, Abidjan, Cote d’Ivoire
Ibrahime Sanogo

Department of Clinical Haematology, University Hospital of Yopougon, Abidjan, Cote d’Ivoire
Antoinette Claire Chiaye Yapo-Crezoit

Immunity Biology Center, Pasteur Institute of Cote d’Ivoire, Abidjan, Cote d’Ivoire

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APA Style

Tatiana Kangah Mireille Kple, Joel Akakpo-Akue, Koffi Adou Mathieu Kra, N’Guessan Bra Yvette Fofie, Koffi Julien Golly, et al. (2021). In Vitro antisickling and Free Radical Scavenging Activities of Kigelia africana (LAM) Stem Bark. Journal of Diseases and Medicinal Plants, 7(1), 1-5. https://doi.org/10.11648/j.jdmp.20210701.11

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Tatiana Kangah Mireille Kple; Joel Akakpo-Akue; Koffi Adou Mathieu Kra; N’Guessan Bra Yvette Fofie; Koffi Julien Golly, et al. In Vitro antisickling and Free Radical Scavenging Activities of Kigelia africana (LAM) Stem Bark. J. Dis. Med. Plants 2021, 7(1), 1-5. doi: 10.11648/j.jdmp.20210701.11

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AMA Style

Tatiana Kangah Mireille Kple, Joel Akakpo-Akue, Koffi Adou Mathieu Kra, N’Guessan Bra Yvette Fofie, Koffi Julien Golly, et al. In Vitro antisickling and Free Radical Scavenging Activities of Kigelia africana (LAM) Stem Bark. J Dis Med Plants. 2021;7(1):1-5. doi: 10.11648/j.jdmp.20210701.11

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@article{10.11648/j.jdmp.20210701.11,
  author = {Tatiana Kangah Mireille Kple and Joel Akakpo-Akue and Koffi Adou Mathieu Kra and N’Guessan Bra Yvette Fofie and Koffi Julien Golly and Ibrahime Sanogo and Antoinette Claire Chiaye Yapo-Crezoit},
  title = {In Vitro antisickling and Free Radical Scavenging Activities of Kigelia africana (LAM) Stem Bark},
  journal = {Journal of Diseases and Medicinal Plants},
  volume = {7},
  number = {1},
  pages = {1-5},
  doi = {10.11648/j.jdmp.20210701.11},
  url = {https://doi.org/10.11648/j.jdmp.20210701.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20210701.11},
  abstract = {Kigelia africana dried bark has been recommended in the management of sickle cell anemia by traditional practitioners. The aim of this study was to evaluate in vitro antioxidant and sickling inhibitory activities of Kigelia africana. Quantitative estimation of phenolic compounds was performed using colorimetric method in hydro-ethanolic extract (70%) and decocted. The sickling inhibitory activity was determinate according to Emmel method and the evaluation of antioxidant properties was carried out using the method of scavenging the free radical DPPH. The obtained results indicated that Kigelia africana species contains flavonoids (8.61±1.08 mg QE and 9.78±1.14 mg QE/g of dry weight) and total phenols (9.48±0.19b mg GAE/g and 11.11±0.22 mg GAE /g of dry weight) in decocted and hydroethanolic extract respectively. The IC50 values of the ethanolic extract and the decocted were 0.320±0.01 and 0.468±0.04 mg/mL respectively. The in vitro sickle-formation inhibition test indicated the value of 89% and 82.36% for the ethanolic extract and the decocted at 10mg/mL respectively. Those values were higher than 80% which was the phenylalanine value. Both extracts showed antioxidant and sickling inhibitory activities. Overall, there could have a correlation between these activities and phenolic compound content in this studied plant extracts. These results would justify the use of this plant in rural environment.},
 year = {2021}
}

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