Genomic Characterization and Identification of Effective Blast Resistant Genes for Sakha 101 and Sakha 108 as High Yielding Egyptian Rice Cultivars

Galal Bakr Anis; Zeinab Abdelnaby Kalboush; Ahmed Ibrahem Elsherif; Raghda Mohamed Sakran

doi:doi:10.11648/j.jps.20221004.14

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Genomic Characterization and Identification of Effective Blast Resistant Genes for Sakha 101 and Sakha 108 as High Yielding Egyptian Rice Cultivars

Galal Bakr Anis, Zeinab Abdelnaby Kalboush, Ahmed Ibrahem Elsherif, Raghda Mohamed Sakran

Published in Journal of Plant Sciences (Volume 10, Issue 4)

Received: 8 April 2022 Accepted: 2 August 2022 Published: 31 August 2022

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Abstract

Sakha 101 is one such short grained common Egyptian rice cultivar and known for its exquisite quality, however, is highly susceptible to blast disease that has led to considerable decline in its area. Sakha 101 was crossed to a blast gene donor line, HR5824-B-3-2-3 and followed through backcross-breeding method that helped to incorporate blast resistance genes and finally Sakha 108 was released as improved version of the most widespread Egyptian commercial rice Sakha 101. The study was to evaluate Sakha 101 and Sakha 108 for high yielding, blast resistance, and effective resistance genes to Pyricularia oryzae as well as assessment of genetic divergence based on genomic in these cultivars. There is a slight increase in the values of Sakha 108 than Sakha 101 in the most of studied traits. Also under this study, seventy isolates were identified as eight main groups i.e., IA, IB, IC, ID, IF, IG, IH and II, but ID group was considered the most common races. On the other hand, Pi-Z and Pii - Pi-k^s resistance genes were the most effective genes to blast fungus. Sakha 108 proved resistance for all tested isolates under greenhouse condition compared with Sakha 101 which exhibited susceptible to 70% of tested isolates. On genomic level, out of 242 markers across the 7 chromosomes; only 6 markers (RM8236, RM13611, RM3839, RM17377, RM160 and RM27154) produced clear fragments and polymorphism between cultivars (Sakha 101 and Sakha 108) and were used to construct a genetic linkage map. A total of 39 candidate genes were identified around their regions on each chromosome. These results enrich our understanding of the differences between Sakha 101 and Sakha 108 and also provide a foundation for selecting candidate for marker-assisted selection breeding in rice.

Published in	Journal of Plant Sciences (Volume 10, Issue 4)
DOI	10.11648/j.jps.20221004.14
Page(s)	150-164
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Rice, Yield, Blast Disease, Resistance Genes, Genomic Regions

References

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Galal Bakr Anis, Zeinab Abdelnaby Kalboush, Ahmed Ibrahem Elsherif, Raghda Mohamed Sakran. (2022). Genomic Characterization and Identification of Effective Blast Resistant Genes for Sakha 101 and Sakha 108 as High Yielding Egyptian Rice Cultivars. Journal of Plant Sciences, 10(4), 150-164. https://doi.org/10.11648/j.jps.20221004.14

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Galal Bakr Anis; Zeinab Abdelnaby Kalboush; Ahmed Ibrahem Elsherif; Raghda Mohamed Sakran. Genomic Characterization and Identification of Effective Blast Resistant Genes for Sakha 101 and Sakha 108 as High Yielding Egyptian Rice Cultivars. J. Plant Sci. 2022, 10(4), 150-164. doi: 10.11648/j.jps.20221004.14

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Galal Bakr Anis, Zeinab Abdelnaby Kalboush, Ahmed Ibrahem Elsherif, Raghda Mohamed Sakran. Genomic Characterization and Identification of Effective Blast Resistant Genes for Sakha 101 and Sakha 108 as High Yielding Egyptian Rice Cultivars. J Plant Sci. 2022;10(4):150-164. doi: 10.11648/j.jps.20221004.14

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@article{10.11648/j.jps.20221004.14,
  author = {Galal Bakr Anis and Zeinab Abdelnaby Kalboush and Ahmed Ibrahem Elsherif and Raghda Mohamed Sakran},
  title = {Genomic Characterization and Identification of Effective Blast Resistant Genes for Sakha 101 and Sakha 108 as High Yielding Egyptian Rice Cultivars},
  journal = {Journal of Plant Sciences},
  volume = {10},
  number = {4},
  pages = {150-164},
  doi = {10.11648/j.jps.20221004.14},
  url = {https://doi.org/10.11648/j.jps.20221004.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20221004.14},
  abstract = {Sakha 101 is one such short grained common Egyptian rice cultivar and known for its exquisite quality, however, is highly susceptible to blast disease that has led to considerable decline in its area. Sakha 101 was crossed to a blast gene donor line, HR5824-B-3-2-3 and followed through backcross-breeding method that helped to incorporate blast resistance genes and finally Sakha 108 was released as improved version of the most widespread Egyptian commercial rice Sakha 101. The study was to evaluate Sakha 101 and Sakha 108 for high yielding, blast resistance, and effective resistance genes to Pyricularia oryzae as well as assessment of genetic divergence based on genomic in these cultivars. There is a slight increase in the values of Sakha 108 than Sakha 101 in the most of studied traits. Also under this study, seventy isolates were identified as eight main groups i.e., IA, IB, IC, ID, IF, IG, IH and II, but ID group was considered the most common races. On the other hand, Pi-Z and Pii - Pi-ks resistance genes were the most effective genes to blast fungus. Sakha 108 proved resistance for all tested isolates under greenhouse condition compared with Sakha 101 which exhibited susceptible to 70% of tested isolates. On genomic level, out of 242 markers across the 7 chromosomes; only 6 markers (RM8236, RM13611, RM3839, RM17377, RM160 and RM27154) produced clear fragments and polymorphism between cultivars (Sakha 101 and Sakha 108) and were used to construct a genetic linkage map. A total of 39 candidate genes were identified around their regions on each chromosome. These results enrich our understanding of the differences between Sakha 101 and Sakha 108 and also provide a foundation for selecting candidate for marker-assisted selection breeding in rice.},
 year = {2022}
}

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TY  - JOUR
T1  - Genomic Characterization and Identification of Effective Blast Resistant Genes for Sakha 101 and Sakha 108 as High Yielding Egyptian Rice Cultivars
AU  - Galal Bakr Anis
AU  - Zeinab Abdelnaby Kalboush
AU  - Ahmed Ibrahem Elsherif
AU  - Raghda Mohamed Sakran
Y1  - 2022/08/31
PY  - 2022
N1  - https://doi.org/10.11648/j.jps.20221004.14
DO  - 10.11648/j.jps.20221004.14
T2  - Journal of Plant Sciences
JF  - Journal of Plant Sciences
JO  - Journal of Plant Sciences
SP  - 150
EP  - 164
PB  - Science Publishing Group
SN  - 2331-0731
UR  - https://doi.org/10.11648/j.jps.20221004.14
AB  - Sakha 101 is one such short grained common Egyptian rice cultivar and known for its exquisite quality, however, is highly susceptible to blast disease that has led to considerable decline in its area. Sakha 101 was crossed to a blast gene donor line, HR5824-B-3-2-3 and followed through backcross-breeding method that helped to incorporate blast resistance genes and finally Sakha 108 was released as improved version of the most widespread Egyptian commercial rice Sakha 101. The study was to evaluate Sakha 101 and Sakha 108 for high yielding, blast resistance, and effective resistance genes to Pyricularia oryzae as well as assessment of genetic divergence based on genomic in these cultivars. There is a slight increase in the values of Sakha 108 than Sakha 101 in the most of studied traits. Also under this study, seventy isolates were identified as eight main groups i.e., IA, IB, IC, ID, IF, IG, IH and II, but ID group was considered the most common races. On the other hand, Pi-Z and Pii - Pi-ks resistance genes were the most effective genes to blast fungus. Sakha 108 proved resistance for all tested isolates under greenhouse condition compared with Sakha 101 which exhibited susceptible to 70% of tested isolates. On genomic level, out of 242 markers across the 7 chromosomes; only 6 markers (RM8236, RM13611, RM3839, RM17377, RM160 and RM27154) produced clear fragments and polymorphism between cultivars (Sakha 101 and Sakha 108) and were used to construct a genetic linkage map. A total of 39 candidate genes were identified around their regions on each chromosome. These results enrich our understanding of the differences between Sakha 101 and Sakha 108 and also provide a foundation for selecting candidate for marker-assisted selection breeding in rice.
VL  - 10
IS  - 4
ER  -

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Author Information

Galal Bakr Anis

Rice Research Department, Field Crops Research Institute, Agricultural Research Center, Kafr El Sheikh, Egypt
Zeinab Abdelnaby Kalboush

Rice Pathology Department, Plant Pathology Research Institute, Agricultural Research Center, Kafr El Sheikh, Egypt
Ahmed Ibrahem Elsherif

Rice Research Department, Field Crops Research Institute, Agricultural Research Center, Kafr El Sheikh, Egypt
Raghda Mohamed Sakran

Rice Research Department, Field Crops Research Institute, Agricultural Research Center, Kafr El Sheikh, Egypt

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Galal Bakr Anis, Zeinab Abdelnaby Kalboush, Ahmed Ibrahem Elsherif, Raghda Mohamed Sakran. (2022). Genomic Characterization and Identification of Effective Blast Resistant Genes for Sakha 101 and Sakha 108 as High Yielding Egyptian Rice Cultivars. Journal of Plant Sciences, 10(4), 150-164. https://doi.org/10.11648/j.jps.20221004.14

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Galal Bakr Anis; Zeinab Abdelnaby Kalboush; Ahmed Ibrahem Elsherif; Raghda Mohamed Sakran. Genomic Characterization and Identification of Effective Blast Resistant Genes for Sakha 101 and Sakha 108 as High Yielding Egyptian Rice Cultivars. J. Plant Sci. 2022, 10(4), 150-164. doi: 10.11648/j.jps.20221004.14

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AMA Style

Galal Bakr Anis, Zeinab Abdelnaby Kalboush, Ahmed Ibrahem Elsherif, Raghda Mohamed Sakran. Genomic Characterization and Identification of Effective Blast Resistant Genes for Sakha 101 and Sakha 108 as High Yielding Egyptian Rice Cultivars. J Plant Sci. 2022;10(4):150-164. doi: 10.11648/j.jps.20221004.14

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@article{10.11648/j.jps.20221004.14,
  author = {Galal Bakr Anis and Zeinab Abdelnaby Kalboush and Ahmed Ibrahem Elsherif and Raghda Mohamed Sakran},
  title = {Genomic Characterization and Identification of Effective Blast Resistant Genes for Sakha 101 and Sakha 108 as High Yielding Egyptian Rice Cultivars},
  journal = {Journal of Plant Sciences},
  volume = {10},
  number = {4},
  pages = {150-164},
  doi = {10.11648/j.jps.20221004.14},
  url = {https://doi.org/10.11648/j.jps.20221004.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20221004.14},
  abstract = {Sakha 101 is one such short grained common Egyptian rice cultivar and known for its exquisite quality, however, is highly susceptible to blast disease that has led to considerable decline in its area. Sakha 101 was crossed to a blast gene donor line, HR5824-B-3-2-3 and followed through backcross-breeding method that helped to incorporate blast resistance genes and finally Sakha 108 was released as improved version of the most widespread Egyptian commercial rice Sakha 101. The study was to evaluate Sakha 101 and Sakha 108 for high yielding, blast resistance, and effective resistance genes to Pyricularia oryzae as well as assessment of genetic divergence based on genomic in these cultivars. There is a slight increase in the values of Sakha 108 than Sakha 101 in the most of studied traits. Also under this study, seventy isolates were identified as eight main groups i.e., IA, IB, IC, ID, IF, IG, IH and II, but ID group was considered the most common races. On the other hand, Pi-Z and Pii - Pi-ks resistance genes were the most effective genes to blast fungus. Sakha 108 proved resistance for all tested isolates under greenhouse condition compared with Sakha 101 which exhibited susceptible to 70% of tested isolates. On genomic level, out of 242 markers across the 7 chromosomes; only 6 markers (RM8236, RM13611, RM3839, RM17377, RM160 and RM27154) produced clear fragments and polymorphism between cultivars (Sakha 101 and Sakha 108) and were used to construct a genetic linkage map. A total of 39 candidate genes were identified around their regions on each chromosome. These results enrich our understanding of the differences between Sakha 101 and Sakha 108 and also provide a foundation for selecting candidate for marker-assisted selection breeding in rice.},
 year = {2022}
}

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TY  - JOUR
T1  - Genomic Characterization and Identification of Effective Blast Resistant Genes for Sakha 101 and Sakha 108 as High Yielding Egyptian Rice Cultivars
AU  - Galal Bakr Anis
AU  - Zeinab Abdelnaby Kalboush
AU  - Ahmed Ibrahem Elsherif
AU  - Raghda Mohamed Sakran
Y1  - 2022/08/31
PY  - 2022
N1  - https://doi.org/10.11648/j.jps.20221004.14
DO  - 10.11648/j.jps.20221004.14
T2  - Journal of Plant Sciences
JF  - Journal of Plant Sciences
JO  - Journal of Plant Sciences
SP  - 150
EP  - 164
PB  - Science Publishing Group
SN  - 2331-0731
UR  - https://doi.org/10.11648/j.jps.20221004.14
AB  - Sakha 101 is one such short grained common Egyptian rice cultivar and known for its exquisite quality, however, is highly susceptible to blast disease that has led to considerable decline in its area. Sakha 101 was crossed to a blast gene donor line, HR5824-B-3-2-3 and followed through backcross-breeding method that helped to incorporate blast resistance genes and finally Sakha 108 was released as improved version of the most widespread Egyptian commercial rice Sakha 101. The study was to evaluate Sakha 101 and Sakha 108 for high yielding, blast resistance, and effective resistance genes to Pyricularia oryzae as well as assessment of genetic divergence based on genomic in these cultivars. There is a slight increase in the values of Sakha 108 than Sakha 101 in the most of studied traits. Also under this study, seventy isolates were identified as eight main groups i.e., IA, IB, IC, ID, IF, IG, IH and II, but ID group was considered the most common races. On the other hand, Pi-Z and Pii - Pi-ks resistance genes were the most effective genes to blast fungus. Sakha 108 proved resistance for all tested isolates under greenhouse condition compared with Sakha 101 which exhibited susceptible to 70% of tested isolates. On genomic level, out of 242 markers across the 7 chromosomes; only 6 markers (RM8236, RM13611, RM3839, RM17377, RM160 and RM27154) produced clear fragments and polymorphism between cultivars (Sakha 101 and Sakha 108) and were used to construct a genetic linkage map. A total of 39 candidate genes were identified around their regions on each chromosome. These results enrich our understanding of the differences between Sakha 101 and Sakha 108 and also provide a foundation for selecting candidate for marker-assisted selection breeding in rice.
VL  - 10
IS  - 4
ER  -

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