Research on the Role of Th17Treg Cells and Their Factors in Graves' Disease with Different Iodine Nutritional Status

Jing Feng; Cuicui Wang; Zhaoxin Mu; Xinsheng Li; Zhenjiang Hou

doi:doi:10.11648/j.sjph.20210901.11

| Peer-Reviewed

Research on the Role of Th17Treg Cells and Their Factors in Graves' Disease with Different Iodine Nutritional Status

Jing Feng, Cuicui Wang, Zhaoxin Mu, Xinsheng Li, Zhenjiang Hou

Published in Science Journal of Public Health (Volume 9, Issue 1)

Received: 18 December 2020 Accepted: Published: 18 January 2021

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

Introduction: At present, studies on the role of iodine nutrition in thyroid function stratification, antibody titer, Th17/Treg cells and related factors in the pathogenesis of GD have not been carried out. Objective: The acritical aims to investigate the correlation between thyroid function and autoantibody titers of Graves' disease (GD) patients with different iodine nutritional status with Th17/Treg cells, their cytokines and transcription factors, and the role of related factors in the pathogenesis of GD. Method: The levels of serum thyroid hormone, autoantibodies and urine iodine in 100 GD patients and 60 healthy subjects are detected by electrochemiluminescence instrument and iodine-catalyzed arsenic-cerium method, respectively. The ratio of Th17 cells to Treg cells and Th17/Treg ratio in peripheral blood mononuclear cells (PBMC) are detected by immunofluorescence-labeled monoclonal antibodies and flow cytometry. Real-time fluorescence quantitative PCR is used to detect the expression levels of retinoic acid-related orphan receptor (ROR-γt) and fork head/wing-shaped spiral transcription factor 3 (Foxp3) mRNA, and the serum IL-17 and TGF-β levels are detected by ELISA. Result: As a result, the proportion of Th17 cells, serum IL-17 and ROR-γt in PBMC of GD patients with different iodine nutritional status significantly increase, while the proportion of Treg cells, the expression of Foxp3mRNA and serum TGF-β significantly decrease. The ratio of Th17/Treg cells in GD patients is significantly positively correlated with the titers of TPOAb and TgAb, and the titers of TPOAb and TgAb antibodies are significantly correlated with Th17/Treg, IL-17 and ROR-γt. Conclusion: In conclusion, thyroid hormones, autoantibodies, Th17, Treg cell ratios and dysfunctions as well as corresponding cytokines and transcription factors in GD patients with different iodine nutritional status participate in the development of GD.

Published in	Science Journal of Public Health (Volume 9, Issue 1)
DOI	10.11648/j.sjph.20210901.11
Page(s)	1-11
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Iodine Nutritional Status, Graves' Disease, Th17 Cells and Treg cells, Cytokines and Transcription Factors

References

[1]	Chen X, Mei Y, He B, et al. General and specific genetic polymorphism of cytokines-related gene in AITD [J]. Mediators Inflamm, 2017, 2017 (1): 3916395.
[2]	XueL, PanC, GuZ, et al. Genetic heterogeneity of susceptibility gene in different ethnic populations: refining association study of PTPN22 for Graves' disease in a Chinese Han population [J]. PLoS One. 2013, 8 (12): e84514.
[3]	Li HX, Xiang N, Hu WK, et al. Relation between therapy options for Graves' disease and the course of Graves'ophthalmopathy: a systematic review and meta-analysis [J]. J EndocrinolInvest, 2016, 39 (11): 1225-33.
[4]	Burch HB, Cooper DS. Management of Graves' disease: A Review [J]. JAMA, 2015, 314 (23): 2544-2554.
[5]	Su JP, Su SO, Zhang B, et al. The effects of different amounts of iodine in take on the immunestatus of patients with Graves’ disease [J]. Clin Med China, 2012, 28 (1): 44-46.
[6]	Hou ZJ, Mu ZX, Wang CC. Research Progress of Th17/Treg Cells and Their Transcription Factors in Autoimmune Diseases [J]. American Journal of Clinical and Experimental Medicine 2019, 7 (4): 83-92.
[7]	Chinese Medical Association Endocrine Credits “China thyroid disease treatment guidelines” Writing Group. Chinese Guidelines for the Diagnosis and Treatment of thyroid diseases-Hyperthyroidism [J]. ChinJ Intern Med, 2007, 46 (10): 876-882.
[8]	Chinese Medical Association Endocrine Credits “China thyroid disease treatment guidelines”Writing Group. China thyroid disease treatment guidelines-iodine deficiency disorders [J]. ChinJ Intern Med, 2008, 47 (8): 689-690.
[9]	Tao LY, Li X, Huang J, et al. Correlative studies of iodine on thyroid nodular diseases [J]. 2013, 23 (27): 82-85.
[10]	Jiang HX, Li YB. Progress of the influence of iodine on thyroid disease and its mechanism [J]. J Chin Pract Diagn Ther, 2016, 30 (7): 639-641.
[11]	Chen ZX, Xu WM, Huang YM, etal. Associations of noniodized salt and thyroid nodule among the Chinese population: a large cross-sectional study [J]. Am J Clin Nutr. 2013, 98 (3): 684-692.
[12]	Fei L, Zhang YL, Wang PY, et al. Analysis of urinary iodinelevels in patients with thyroiddiseases in Yuhuan county [J]. Chin JEpidem, 2010, 31 (2): 239-240.
[13]	Wu ZF, Liang Y, Chen XQ. Investigation of urinary iodine in patients with thyroid disease [J]. Youjiang-medi, 2010, 38 (2): 188-189.
[14]	Huang SY, Yan YM, Wu M, et al. Analysis of Urinary Iodine Content of Newly Diagnosed Thyroid Disease Patients in Xiaogan [J]. Guangzhou Microelement Science 2015, 22 (1): 24-27.
[15]	Xu L, Chen WH. Analysis of the difference of urinary iodine levels among patients with different types of thyroid diseases [J] J Anhui Med, 2020, 14 (7): 785-787.
[16]	Fan X, Chen SK. Relationship of Iodine Excessiveness and Thyroid Function [J]. Med Reca, 2011, 17 (14): 2165-2167.
[17]	Tan X, Cao XX, He L. Effect of iodine excess on thyroid function and its pathogenesis of diseases [J]. Chin J Ctrl Endem Dis, 2019, 34 (1): 35-37.
[18]	Yang F, Teng WP, Shan ZY, et al. A comparative epidemiologic survey of hyperthyroidism in areas with different iodine intake [J]. Chin J Endocrinol Metab, 2001, 17 (4): 197-201.
[19]	Li CQ, Yin HQ, Zhang CK, et al. Epidemiological investigation of Graves's disease in one hundred thousand peoplein Daqing area [J]. National Medical Journal of China, 1996, 76 (6): 443-446.
[20]	Shan Z, Chen L, Lian X, et al. Iodine status and prevalence of thyroid disorders after introduction of mandatory universal saltiodization for 16 years in China: a crosssectional study in 10cities [J]. Thyroid 2016, 26 (8): 1125-1130.
[21]	Jin Y, Teng WP, Yuan B, et al. Thyroid autoimmunity in members from multiplex families with Graves' disease and effect of iodine intake on its incidence [J] Chin J Endocrinol Metab, 2001, 17 (2): 79-82.
[22]	Yan SL, Wang YG, Wang F, et al. Relationship between iodine in urine and Graves's disease along coastal district in shading [J]. Chin J Endem, 2004, 23 (3): 245-247.
[23]	Cong JN, Zhao YJ, Li G, et al. The changes of SOD and MDA in serum of patients with autoimmune thyroid disease and the relationship between SOD and urine iodine Guangzhou Microelement Science, 2011, 18 (8): 12-15.
[24]	Chen XM, Guo ZQ, Zhou F, et al. Correlation of concentrations of iodine in urine with Graves’ disease along coastal area of western Guangdong [J]. China Tropi Med, 2010, 10 (8): 922-923.
[25]	Chen XY, Zhang ZT, Deng SY, et al. Clinical manifestation of Graves’ disease patients with different levels of thyroid related autoantibodies [J]. New Med, 2011, 42 (12): 789-792.
[26]	Huang YZ, Tang BY, Chang L, et al. Influencing factors on goiter and thyroid eye in patients with Graves' disease [J] J Anhui Med, 2015, 36 (5): 580-582.
[27]	Li L, Liu YM, Chen TS, et al. Expression of serum IL-17 in Graves' disease patients [J]. J Guangdong Pharm Univ, 2014, 30 (3): 363-365.
[28]	Li HL, Deng ZK, Zheng YH, et al. Expression of Th17 cells and related cytokines in peripheral blood of patients with Graves' disease [J]. J Immun Chin 2016, 32 (4): 563-566.
[29]	Ding JY, Qin LD, Xu Y, et al. Expression and Significance of PD-1/PD-L1 on CD4⁺CD25⁺Treg Cells in Peripheral Blood of Patients with Graves’ Disease [J]. Prog Mode Biom, 2019, 19 (4): 718-723.
[30]	Ma, JG, Wei HL, Shang H. Correlational between peripheral blood T cell subsets and thyroid function and thyroid autoantibodies in patients with Graves’ disease [J]. Int J Endocrinol Metab, 2020, 40 (1): 21-26.
[31]	Huang J, Yu PF, Yang ZP. Clinical application of thyroid-stimulating hormone and thyroid autoantibodies in the diagnosis of thyroid disease [J]. Chin J Ctrl Endem Dis, 2015, 30 (1): 65-66.
[32]	Dong X. Diagnostic value of serum thyroid autoantibodies in patients with autoimmune thyroid disease [J] Clin Med, 2018, 38 (5): 40-41.
[33]	Yang J, Li Q. Clinical application of thyroid stimulating hormone and thyroid autoantibodies in the diagnosis of thyroid diseases [J]. Proc Clin Med, 2018, 27 (6): 424-426.
[34]	Bossowski A, Moniuszko M, Idkowska E, et al. Decreased proportions of CD4⁺IL17⁺/CD4⁺CD25⁺CD127^- and CD4⁺IL17⁺/CD4⁺CD25⁺CD127^-FoxP3⁺T cells in children with autoimmune thyroid diseases [J]. Auto-immunity, 2016, 49 (5): 320-328.
[35]	Marazuela M, García-López MA, Figueroavega N, et al. Regulatory T Cells in Human Autoimmune Thyroid Disease [J]. J Clin Endocrinol Metab, 2006, 91 (9): 3639-3646.
[36]	Figueroa-Vega N, Alfonso-Pérez M, Benedicto I, et al. Increased circulating pro-inflammatory cytokines and Th17 lymphocytes in Hashimoto's thyroiditis [J]. J Clin Endocrinol Metab, 2010, 95 (2): 953-962.
[37]	Chen ZJ, Liu C, Li Q, et al. The change of Th17 lymphocytes and cytokines in autoimmune thyroid diseases [J]. Immu J, 2011, 27 (9): 785-788.
[38]	Zhang DH, Qiu XG, Li JH, et al. MiR-23a-3p-regulated abnormal acetylation of FoxP3 induces regulatory T cell function defect in Graves' disease [J]. Biol Chem. 2019, 24; 400 (5): 639-650.
[39]	Li JR, Hong FY, Zeng JY, et al. Functional interleukin-17 receptor A are present in the thyroid gland in intractable Graves’ disease [J]. Cell Immunol. 2013, 281 (1): 85-90.
[40]	Zhou XJ, Li WP. Relationship between CD4⁺CD25⁺FoxP3⁺T cell regulatory T cells and Th17 cells and Graves’ disease [J]. J Taishan Medical College, 2019, 40 (12): 907-908.
[41]	Li CH, Chen ZL, Sun QK, et al. The mechanistic study of CD4⁺CD25⁺regulatory T and Th17 cells on Graves disease [J]. Northern Pharm, 2015, 12 (5): 123-124.
[42]	Zheng L, Chen ZJ, Liu C. Changes of Th3 and Th17 lymphocytes and related cytokines in patients with autoimmune thyroid disease [J]. China J Immu, 2013, 29 (1): 43-47.
[43]	Gao ST, Zhu TN, Zhang YN, et al. The frequency and function of CD4⁺CD25⁺regulatory T cells in autoimmune thyroid diseases [J]. China J Immu, 2011, 27 (3): 269-273.
[44]	Mao C, Wang S, Xiao Y, et al. Impairment of regulatory capacity of CD4⁺CD25⁺regulatory T cells mediated by dendritic cell polarization and hyperthyroidism in Graves' disease [J]. JImmunol. 2011, 186 (8): 4734-4743.
[45]	Zheng LT, Yu SP, Wang N, et al. Regulatory T cells and TGF-β1 in autoimmunetherole of thyroid disease [J]. Shandong Med 2012, 52 (1): 81-82.
[46]	Huang XQ, Liu C. Effect of IL-21 on Treg cells in peripheral blood mononuclear cells in pathogenesis of Graves’ disease [J]. Chin J Immu, 2016, 32 (6): 853-857, 862.
[47]	Qin J, Zhou J, Fan CL, et al. Increased Circulating Th17 but Decreased CD4⁺Foxp3⁺Treg and CD19⁺ CD1d hi CD5⁺Breg Subsets in New-Onset Graves' Disease [J]. Biomed Res Int. 2017, 2017: 8431838.
[48]	Pawlowski P, Grubczak K, Kostecki J, et al. Decreased Frequencies of Peripheral Blood CD4⁺CD25⁺CD. 127^-Foxp3⁺.
[49]	in Patients with Graves' Disease and Graves' Orbitopathy: Enhancing effect of Insulin Growth Factor-1 on Treg Cells [J]. HormMetab Res, 2017, 49 (3): 185-191.
[50]	Tan Y, Chen W, Liu C, et al. Effect of IL-21 on the Balance of Th17 Cells/Treg Cells in the Pathogenesis of Graves' Disease [J]. Endocr Res. 2019, 44 (4): 138-147.
[51]	Glick AB, Wodzinski A, Fu P, et al. Impairment of regulatory T-cell function in autoimmune thyroid disease [J]. Thyroid, 2013, 23 (7): 871-878.
[52]	Du J, Wang X, Tan GQ, et al. Research progress on the Graves’ disease susceptibility genes [J]. Chin J Comp Med, 2019, 29 (4): 126-132.
[53]	Caturegli P, De Remigis A, Chuang K, et al. Hashimoto's thyroiditis: celebrating the centennial through the lens of the Johns Hopkins hospital surgical pathology records [J]. Thyroid. 2013, 23 (2): 142-150.
[54]	Latrofa F, Fiore E, Rago T, et al. Iodine contributes to thyroid autoimmunity in humans by unmasking a cryptic epitope on thyroglobulin [J]. J Clin Endocrinol Metab, 2013, 98 (11): E1768-1774.
[55]	Zhu JF, Yamane H, Paul WE. Differentiation of effector CD4 T cell populations [J]. Annu Rev Immunol. 2010, 28: 445-489.
[56]	Gonza´lez-Amaro R, Marazuela M. T regulatory (Treg) and T helper 17 (Th17) lymphocytes in thyroid autoimmunity [J]. Endocrine, 2015, 52 (1): 1-9.
[57]	Kleinewietfeld M, Hafler DA. The plasticity of human Treg and Th17 cells and its role in autoimmunity [J]. Semin Immunol. 2013, 25 (4): 305-312.
[58]	Huang F, Bi JH, Hao LX, et al. Detection and clinical significance of peripheral blood CD4⁺CD25⁺Foxp 3⁺regulatory t cells and urinary iodine in patients with Graves's disease [J]. HeBei Med, 2017, 23 (1): 147-149.
[59]	Wang H, Zhao S, Tang X, et al. Changes of regulatory T cells in Graves' disease [J]. Huazhong Univ Sci Technolog Med Sci, 2006, 26 (5): 545-547.
[60]	Xue HB, Ma L, Li YB, et al. Correlation between Treg/Th17 cells imbalance andautoimmunity in Hashi-moto's thyroiditis [J]. Chin JMode Med, 2012, 22 (23): 67-71.
[61]	PyzikA, GrywalskaE, Matyjaszek-MatuszekB, et al. Immune disorders in Hashimoto's thyroidditis: what do we know so far? [J]. J Immunol Res. 2015, 2015: 979167.
[62]	González-AmaroR, Marazuela M. T regulatory (Treg) and T helper17 (Th17) lymphocytes in thyroid autoimmunity [J]. Endocrine. 2016, 52 (1): 30-38.
[63]	Li C, Ebert PJ, Li QJ. T cell receptor (TCR) and transforming growth factor β (TGF-β) signaling converge on DNA (cytosine-5)-methyltransferase to control forkhead box protein 3 (foxp3) locus methyl-and inducible regulatory T cell differentiation [J]. J Biol Chem. 2013, 288 (26): 19127-19139.
[64]	Cai H, Kong W, Zhou T, et al. Radiofrequency ablation versus reresection in treating recurrent hepatocellular carcinoma: a meta-analysis [J]. Medicine, 2014, 93 (22): e122
[65]	Hirota K, Martin B, Veldhoen M. Development, regulation and functional capacities of Th17 cells [J]. Sem in Immunopathol, 2010, 32 (1): 3-16.
[66]	Khan U, Ghazanfar H. T Lymphocytes and Autoimmunity [J]. Int Rev Cell Mol Biol. 2018, 341: 125-168.

Cite This Article

Plain Text BibTeX RIS

APA Style

Jing Feng, Cuicui Wang, Zhaoxin Mu, Xinsheng Li, Zhenjiang Hou. (2021). Research on the Role of Th17Treg Cells and Their Factors in Graves' Disease with Different Iodine Nutritional Status. Science Journal of Public Health, 9(1), 1-11. https://doi.org/10.11648/j.sjph.20210901.11

Copy | Download

ACS Style

Jing Feng; Cuicui Wang; Zhaoxin Mu; Xinsheng Li; Zhenjiang Hou. Research on the Role of Th17Treg Cells and Their Factors in Graves' Disease with Different Iodine Nutritional Status. Sci. J. Public Health 2021, 9(1), 1-11. doi: 10.11648/j.sjph.20210901.11

Copy | Download

AMA Style

Jing Feng, Cuicui Wang, Zhaoxin Mu, Xinsheng Li, Zhenjiang Hou. Research on the Role of Th17Treg Cells and Their Factors in Graves' Disease with Different Iodine Nutritional Status. Sci J Public Health. 2021;9(1):1-11. doi: 10.11648/j.sjph.20210901.11

Copy | Download

@article{10.11648/j.sjph.20210901.11,
  author = {Jing Feng and Cuicui Wang and Zhaoxin Mu and Xinsheng Li and Zhenjiang Hou},
  title = {Research on the Role of Th17Treg Cells and Their Factors in Graves' Disease with Different Iodine Nutritional Status},
  journal = {Science Journal of Public Health},
  volume = {9},
  number = {1},
  pages = {1-11},
  doi = {10.11648/j.sjph.20210901.11},
  url = {https://doi.org/10.11648/j.sjph.20210901.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjph.20210901.11},
  abstract = {Introduction: At present, studies on the role of iodine nutrition in thyroid function stratification, antibody titer, Th17/Treg cells and related factors in the pathogenesis of GD have not been carried out. Objective: The acritical aims to investigate the correlation between thyroid function and autoantibody titers of Graves' disease (GD) patients with different iodine nutritional status with Th17/Treg cells, their cytokines and transcription factors, and the role of related factors in the pathogenesis of GD. Method: The levels of serum thyroid hormone, autoantibodies and urine iodine in 100 GD patients and 60 healthy subjects are detected by electrochemiluminescence instrument and iodine-catalyzed arsenic-cerium method, respectively. The ratio of Th17 cells to Treg cells and Th17/Treg ratio in peripheral blood mononuclear cells (PBMC) are detected by immunofluorescence-labeled monoclonal antibodies and flow cytometry. Real-time fluorescence quantitative PCR is used to detect the expression levels of retinoic acid-related orphan receptor (ROR-γt) and fork head/wing-shaped spiral transcription factor 3 (Foxp3) mRNA, and the serum IL-17 and TGF-β levels are detected by ELISA. Result: As a result, the proportion of Th17 cells, serum IL-17 and ROR-γt in PBMC of GD patients with different iodine nutritional status significantly increase, while the proportion of Treg cells, the expression of Foxp3mRNA and serum TGF-β significantly decrease. The ratio of Th17/Treg cells in GD patients is significantly positively correlated with the titers of TPOAb and TgAb, and the titers of TPOAb and TgAb antibodies are significantly correlated with Th17/Treg, IL-17 and ROR-γt. Conclusion: In conclusion, thyroid hormones, autoantibodies, Th17, Treg cell ratios and dysfunctions as well as corresponding cytokines and transcription factors in GD patients with different iodine nutritional status participate in the development of GD.},
 year = {2021}
}

Copy | Download

TY  - JOUR
T1  - Research on the Role of Th17Treg Cells and Their Factors in Graves' Disease with Different Iodine Nutritional Status
AU  - Jing Feng
AU  - Cuicui Wang
AU  - Zhaoxin Mu
AU  - Xinsheng Li
AU  - Zhenjiang Hou
Y1  - 2021/01/18
PY  - 2021
N1  - https://doi.org/10.11648/j.sjph.20210901.11
DO  - 10.11648/j.sjph.20210901.11
T2  - Science Journal of Public Health
JF  - Science Journal of Public Health
JO  - Science Journal of Public Health
SP  - 1
EP  - 11
PB  - Science Publishing Group
SN  - 2328-7950
UR  - https://doi.org/10.11648/j.sjph.20210901.11
AB  - Introduction: At present, studies on the role of iodine nutrition in thyroid function stratification, antibody titer, Th17/Treg cells and related factors in the pathogenesis of GD have not been carried out. Objective: The acritical aims to investigate the correlation between thyroid function and autoantibody titers of Graves' disease (GD) patients with different iodine nutritional status with Th17/Treg cells, their cytokines and transcription factors, and the role of related factors in the pathogenesis of GD. Method: The levels of serum thyroid hormone, autoantibodies and urine iodine in 100 GD patients and 60 healthy subjects are detected by electrochemiluminescence instrument and iodine-catalyzed arsenic-cerium method, respectively. The ratio of Th17 cells to Treg cells and Th17/Treg ratio in peripheral blood mononuclear cells (PBMC) are detected by immunofluorescence-labeled monoclonal antibodies and flow cytometry. Real-time fluorescence quantitative PCR is used to detect the expression levels of retinoic acid-related orphan receptor (ROR-γt) and fork head/wing-shaped spiral transcription factor 3 (Foxp3) mRNA, and the serum IL-17 and TGF-β levels are detected by ELISA. Result: As a result, the proportion of Th17 cells, serum IL-17 and ROR-γt in PBMC of GD patients with different iodine nutritional status significantly increase, while the proportion of Treg cells, the expression of Foxp3mRNA and serum TGF-β significantly decrease. The ratio of Th17/Treg cells in GD patients is significantly positively correlated with the titers of TPOAb and TgAb, and the titers of TPOAb and TgAb antibodies are significantly correlated with Th17/Treg, IL-17 and ROR-γt. Conclusion: In conclusion, thyroid hormones, autoantibodies, Th17, Treg cell ratios and dysfunctions as well as corresponding cytokines and transcription factors in GD patients with different iodine nutritional status participate in the development of GD.
VL  - 9
IS  - 1
ER  -

Copy | Download

Author Information

Jing Feng

Cangzhou Central Hospital, Cangzhou, China
Cuicui Wang

Institute of Thyroid Diseases Affiliated to Cangzhou Medical College, Cangzhou Thyroid Disease Engineering Technology Research Center, Cangzhou, China
Zhaoxin Mu

Institute of Thyroid Diseases Affiliated to Cangzhou Medical College, Cangzhou Thyroid Disease Engineering Technology Research Center, Cangzhou, China
Xinsheng Li

Cangzhou Central Hospital, Cangzhou, China
Zhenjiang Hou

Institute of Thyroid Diseases Affiliated to Cangzhou Medical College, Cangzhou Thyroid Disease Engineering Technology Research Center, Cangzhou, China

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Jing Feng, Cuicui Wang, Zhaoxin Mu, Xinsheng Li, Zhenjiang Hou. (2021). Research on the Role of Th17Treg Cells and Their Factors in Graves' Disease with Different Iodine Nutritional Status. Science Journal of Public Health, 9(1), 1-11. https://doi.org/10.11648/j.sjph.20210901.11

Copy | Download

ACS Style

Jing Feng; Cuicui Wang; Zhaoxin Mu; Xinsheng Li; Zhenjiang Hou. Research on the Role of Th17Treg Cells and Their Factors in Graves' Disease with Different Iodine Nutritional Status. Sci. J. Public Health 2021, 9(1), 1-11. doi: 10.11648/j.sjph.20210901.11

Copy | Download

AMA Style

Jing Feng, Cuicui Wang, Zhaoxin Mu, Xinsheng Li, Zhenjiang Hou. Research on the Role of Th17Treg Cells and Their Factors in Graves' Disease with Different Iodine Nutritional Status. Sci J Public Health. 2021;9(1):1-11. doi: 10.11648/j.sjph.20210901.11

Copy | Download

@article{10.11648/j.sjph.20210901.11,
  author = {Jing Feng and Cuicui Wang and Zhaoxin Mu and Xinsheng Li and Zhenjiang Hou},
  title = {Research on the Role of Th17Treg Cells and Their Factors in Graves' Disease with Different Iodine Nutritional Status},
  journal = {Science Journal of Public Health},
  volume = {9},
  number = {1},
  pages = {1-11},
  doi = {10.11648/j.sjph.20210901.11},
  url = {https://doi.org/10.11648/j.sjph.20210901.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjph.20210901.11},
  abstract = {Introduction: At present, studies on the role of iodine nutrition in thyroid function stratification, antibody titer, Th17/Treg cells and related factors in the pathogenesis of GD have not been carried out. Objective: The acritical aims to investigate the correlation between thyroid function and autoantibody titers of Graves' disease (GD) patients with different iodine nutritional status with Th17/Treg cells, their cytokines and transcription factors, and the role of related factors in the pathogenesis of GD. Method: The levels of serum thyroid hormone, autoantibodies and urine iodine in 100 GD patients and 60 healthy subjects are detected by electrochemiluminescence instrument and iodine-catalyzed arsenic-cerium method, respectively. The ratio of Th17 cells to Treg cells and Th17/Treg ratio in peripheral blood mononuclear cells (PBMC) are detected by immunofluorescence-labeled monoclonal antibodies and flow cytometry. Real-time fluorescence quantitative PCR is used to detect the expression levels of retinoic acid-related orphan receptor (ROR-γt) and fork head/wing-shaped spiral transcription factor 3 (Foxp3) mRNA, and the serum IL-17 and TGF-β levels are detected by ELISA. Result: As a result, the proportion of Th17 cells, serum IL-17 and ROR-γt in PBMC of GD patients with different iodine nutritional status significantly increase, while the proportion of Treg cells, the expression of Foxp3mRNA and serum TGF-β significantly decrease. The ratio of Th17/Treg cells in GD patients is significantly positively correlated with the titers of TPOAb and TgAb, and the titers of TPOAb and TgAb antibodies are significantly correlated with Th17/Treg, IL-17 and ROR-γt. Conclusion: In conclusion, thyroid hormones, autoantibodies, Th17, Treg cell ratios and dysfunctions as well as corresponding cytokines and transcription factors in GD patients with different iodine nutritional status participate in the development of GD.},
 year = {2021}
}

Copy | Download

TY  - JOUR
T1  - Research on the Role of Th17Treg Cells and Their Factors in Graves' Disease with Different Iodine Nutritional Status
AU  - Jing Feng
AU  - Cuicui Wang
AU  - Zhaoxin Mu
AU  - Xinsheng Li
AU  - Zhenjiang Hou
Y1  - 2021/01/18
PY  - 2021
N1  - https://doi.org/10.11648/j.sjph.20210901.11
DO  - 10.11648/j.sjph.20210901.11
T2  - Science Journal of Public Health
JF  - Science Journal of Public Health
JO  - Science Journal of Public Health
SP  - 1
EP  - 11
PB  - Science Publishing Group
SN  - 2328-7950
UR  - https://doi.org/10.11648/j.sjph.20210901.11
AB  - Introduction: At present, studies on the role of iodine nutrition in thyroid function stratification, antibody titer, Th17/Treg cells and related factors in the pathogenesis of GD have not been carried out. Objective: The acritical aims to investigate the correlation between thyroid function and autoantibody titers of Graves' disease (GD) patients with different iodine nutritional status with Th17/Treg cells, their cytokines and transcription factors, and the role of related factors in the pathogenesis of GD. Method: The levels of serum thyroid hormone, autoantibodies and urine iodine in 100 GD patients and 60 healthy subjects are detected by electrochemiluminescence instrument and iodine-catalyzed arsenic-cerium method, respectively. The ratio of Th17 cells to Treg cells and Th17/Treg ratio in peripheral blood mononuclear cells (PBMC) are detected by immunofluorescence-labeled monoclonal antibodies and flow cytometry. Real-time fluorescence quantitative PCR is used to detect the expression levels of retinoic acid-related orphan receptor (ROR-γt) and fork head/wing-shaped spiral transcription factor 3 (Foxp3) mRNA, and the serum IL-17 and TGF-β levels are detected by ELISA. Result: As a result, the proportion of Th17 cells, serum IL-17 and ROR-γt in PBMC of GD patients with different iodine nutritional status significantly increase, while the proportion of Treg cells, the expression of Foxp3mRNA and serum TGF-β significantly decrease. The ratio of Th17/Treg cells in GD patients is significantly positively correlated with the titers of TPOAb and TgAb, and the titers of TPOAb and TgAb antibodies are significantly correlated with Th17/Treg, IL-17 and ROR-γt. Conclusion: In conclusion, thyroid hormones, autoantibodies, Th17, Treg cell ratios and dysfunctions as well as corresponding cytokines and transcription factors in GD patients with different iodine nutritional status participate in the development of GD.
VL  - 9
IS  - 1
ER  -

Copy | Download