Abstract: Biochemical experimental teaching is an indispensable key link in the process of professional training in the college of life sciences. To develop comprehensive and designing experiments courses can improve the quality of biochemical experiment teaching. Fusion protein expression and purification technology is an important research means to research protein structure and function. In the field of protein science, getting high purity and bioactive proteins often is the basis of protein structure and function studies. Affinity chromatography is one of effective methods of protein separation and purification. Which fusion protein purification technology acts as the core, our teaching platform has opened an innovative, comprehensive experimental teaching course called "Purification of His-tagged proteins using Ni2+ Sepharose affinity chromatography ". This paper expounds the implementation of the teaching goal, the key and difficult, experimental principle, experimental procedures and results, etc. Practice shows that the experimental teaching course can subtly make an organic integration of multi-disciplinary knowledge points, just like biochemistry, molecular biology, gene engineering technology and so on. Using this strategy, we can not only consolidate and strengthen the basic knowledge of biochemistry theory students have studied, but also can improve their comprehensive abilities to experimental designs and cultivate their innovative spirits meanwhile. The innovative experiment course, with moderate difficulty, can be widely applied in the other colleges those have biochemistry major.Abstract: Biochemical experimental teaching is an indispensable key link in the process of professional training in the college of life sciences. To develop comprehensive and designing experiments courses can improve the quality of biochemical experiment teaching. Fusion protein expression and purification technology is an important research means to researc...Show More
