Abstract: Coptis chinensis is a famous medicinal plant, which belongs to Coptis of Ranunculaceae. In China it has been applied for more than 2,000 years, and it is also one of the key medicinal plants worldwide with the whole plants and extract. The genome of Coptis chinensis was investigated in order to accelerate the completion of its genome sequencing, promote its research in molecular breeding, genetic evolution and the excavation of important medicinal plant gene resources. Based on high-throughput sequencing technology, two libraries with fragment sequences about 270 bp were constructed. After performing double-terminal 270 bp (PE 270) sequencing, 49.82 Gb raw data was obtained. It was estimated that Coptis chinensis genome size was 1.06 Gb, the repeat sequence content was about 71.46%, the heterozygosity was about 0.24%, and the GC content was about 39.50%. The study showed that Coptis chinensis had complex geome with repeat and large sequence data. Therefore, three generation sequencing technology is recommended to construcut a 20 Kb library and test 100× Pacbio data, and construcut the 270 bp small library and test 50× illumina data, which will contribute to the acquisiton of high-quality complete genome map of Coptis chinensis. This study also provides basic data for mining functional genes of C. chinensis.Abstract: Coptis chinensis is a famous medicinal plant, which belongs to Coptis of Ranunculaceae. In China it has been applied for more than 2,000 years, and it is also one of the key medicinal plants worldwide with the whole plants and extract. The genome of Coptis chinensis was investigated in order to accelerate the completion of its genome sequencing, pr...Show More
