Advances in Biochemistry

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Hepatorenal Protective Activities of Plantain Root (Musa paradisiaca) on Arsenic-induced Oxidative Damage in Rat

Received: 08 July 2015    Accepted: 28 July 2015    Published: 05 August 2015
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Abstract

The protective role of aqueous root extract of Musa paradisiaca on arsenic chloride-induced oxidative damage in the liver and kidney of albino rats was investigated in this study. Twenty four albino rats were grouped in to four (A, B, C and D). Group A served as the control and received distilled water while B, C and D were administered 10 mg/kg bw of arsenic chloride weekly. Groups C and D were treated with 200 and 500 mg/kg bw of aqueous extract of Musa paradisiaca roots respectively for 28 days while group B was left untreated. Phytochemical screening carried out on the root powder indicated the presence of tannins, terpenoids, steroids, sarponins, cardiac glycosides and flavonoids. Arsenic chloride induced a significant elevation in aminotransferases (ALT and AST), ALP and total bilirubin and reduction in serum protein and albumin indicating derangement of liver function. Significant elevation of serum creatinine, urea, uric acid, blood urea nitrogen and electrolytes levels were also recorded in arsenic intoxicated rats indicating disruption of kidney function. Histological examination of the kidney and liver of arsenic intoxicated rats also indicated significant alteration in tissue architecture and morphology. There was significant increase in the liver and kidney weight index in arsenic treated groups compared to the control indicating tissue inflammation. Treatment of rats with different doses of Musa paradisiaca root extract significantly (P<0.05) normalized liver and kidney functions while it also restored normal tissue histology at the end of the experiment. It can be concluded that Musa paradisiaca contain bioactive constituents capable of protecting the living system against arsenic-induced disruption of liver and kidney functions in rats.

DOI 10.11648/j.ab.20150302.12
Published in Advances in Biochemistry (Volume 3, Issue 2, April 2015)
Page(s) 30-34
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Musa paradisiaca, Arsenic Chloride, Liver Function, Kidney Function

References
[1] Wadei HM, Mai ML, Ahsan N, Gonwa TA. Hepatorenal syndrome: Pathophysiology and management. Clin. J. Am. Soc. Nephro. 2006; 1 (5):1066-1079.
[2] Cotran RS, Kumar N Fausho F, Nelson SL, Abbas AK. Robbins and Cotran pathological basis of disease. St Louis MO: Elsevier Saunders. 2005; pp: 878.
[3] Agency for Toxic Substances and Disease Registry-ASTDR. Toxicological profiles for arsenic. American publishing press. Atlanta. USA. 2005; pp:228.
[4] Ravenscroft P. Predicting the global distribution of arsenic pollution in ground water. Royal Geographic Soc. J. 2007; 25(2):45-59.
[5] Kharil I, Abedul H, Abdul F. Dose response relationship between arsenic exposure and the serum enzymes for liver function test in the individuals exposed to arsenic: Environ. Health J. 2011; 10 (64):102-115.
[6] Hughes MF. Accumulation and metabolism of arsenic in mice after repeated oral administration of arsenate. Toxicol. & Applied Pharmacol. J. 2002; 191(3):202-210.
[7] Sharma SK, Gupta J. Plants having hepatoprotective activity. Phytochem. & Pharmacol. J. 2002; 2:253-270.
[8] Capen RG. Food consumption table. East Asian Journal of Nutrition. 1990; 12(2):15-20.
[9] Ahlborn H, Henderson S, Davies N. No immediate pain relief for the pharmaceutical industry. Curriculum Opin. Drug discovery Development. 2005; 8(3):334-391.
[10] Harborne JB. Phytochemical methods. London Chapman and Hall Ltd. Pp. 49- 188. Moss DW, Rosalki SB (1996). Enzyme tests in diagnosis. Edward Arnold. London. 1973; pp. 68-77.
[11] Bancroft JD, Stevens A. Theory and Practice of Histological Techniques. Churchill Livingstone. 2nd ed. London. 1982; pp 95-108.
[12] Donninger LP, Hulson DH, Pickering BA. Modified biuret method of protein estimation. Biochem. J. 1972; 216:701-707.
[13] Doumas BT, Watson WA, Biggs HG. Albumin standards and the measurement of serum albumin with bromocresol green. Clin. Chim. 1971; Acta 31:87-96.
[14] Reitman S, Frankel S. Determination of glutamate-pyruvate transaminase (ALT) and Aspartate aminotransferase (AST). J. Clin. Pathol. 1957; 28:56.
[15] Wright PJ, Plummer DT, Leathwood PT. Enzyme in rat urine. Alkaline phosphatase. Enzymologia. 1972; 42:317-327.
[16] Jendrassik L, Groft P. Colorimetric methods of determination of bilirubin. Biochem. 1938; 2:81-82.
[17] Veniamin MP, Vakirtzi-Lemonia C. Chemical bases of the carbamidodiacetyl micro method for estimation of urea, citruline and carbamyl derivatives. Clin. Chem. 1970; 16:3-6.
[18] Fischbach FT, Dunning MB. Manual of laboratory and diagnostic test, 8th edition, Philadelphia: Lippincott Williams and Wilkins. 2009; pp: 250.
[19] Tietz NW. Clinical guide to laboratory test (1st edition). Saunders Publication, London-UK. 1983; pp 135:148.
[20] Friedman PBC. Effect of diseases on clinical laboratory test. Am. Soc. Clin. Chem. J. 1980; 5(2):122-125.
[21] Moss DW, Rosalki SBR. Enzyme tests in Diagnosis. 2nd edition. Edward Arnold. London. 1996; pp 201-213.
[22] Tseng CH. Urinary arsenic methylation in animals. Journal of Environ. Sci. Health Care. 2007; 25(1):1-22.
[23] Tseng CH, Chong CK, Tseng CP. Long-term arsenic exposure and ischemic heart disease in arseniasis-hyperendemic villages in Taiwan. Toxicol. Lett. 2003; 137:1–2.
[24] Guyton AC, Hall JE. A textbook of medical physiology 10th Edition., W.B. Saunders Co., Philadelphia, 2000; pp 382-401.
[25] Schmidt E, Schmidt FW. Enzyme diagnosis in diseases of the liver and biliary system. Adv. Clin. Enzymol J. 1979; 1:239-242.
[26] Sanjiv C. The liver book: a complementary guide to diagnosis, treatment and recovery (2nd edition). Atria Jimcafe Company. 2002; pp 86.
[27] Cameron JS, Greger R. Renal function and testing of function. Oxford Textbook Clin. Nephrol. 1998; pp 36–39.
[28] Wang W, Srivastava S. “Serological markers” In Breslow, Lester Encyclopedia of Public Health. NY. Macmillan, USA. 2002; pp 1088-1090.
[29] Oyewole OI, Oladipupo OT, Atoyebi BV. Assessment of renal and hepatic functions in rats administered methanolic leaf extract of Jatropha tanjorensis. Annals of Biol. Research. 2012; 3 (2): 837-841.
[30] Ibegbu AO, Okonji WO, Umana DJ, Musa SA. Anti-inflammatory effects of the aqueous extract of plantain roots. Brit. J. Pharmacol. & Toxicol. 2012; 3 (2): 70-75.
[31] Lewis DL, Shaw GP. A natural flavonoid present in unripe plantain pulp (Musa sapientum L.V. paradisiaca).Protects the gastric mucosa from aspirin-induced erosions. J. Ethnopharmacol. 2001; 65:283-288.
Author Information
  • Department of Biochemistry, Osun State University, Osogbo, Nigeria

  • Department of Biochemistry, Federal University of Technology, Akure, Nigeria

  • Department of Biochemistry, Osun State University, Osogbo, Nigeria

  • Department of Biochemistry, Osun State University, Osogbo, Nigeria

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    Oluwole Israel Oyewole, Tolulope Olufunmilayo Akinbamijo, Oluwabukola Joy Omoboriowo, Naomi Ubongabasi Ukana. (2015). Hepatorenal Protective Activities of Plantain Root (Musa paradisiaca) on Arsenic-induced Oxidative Damage in Rat. Advances in Biochemistry, 3(2), 30-34. https://doi.org/10.11648/j.ab.20150302.12

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    Oluwole Israel Oyewole; Tolulope Olufunmilayo Akinbamijo; Oluwabukola Joy Omoboriowo; Naomi Ubongabasi Ukana. Hepatorenal Protective Activities of Plantain Root (Musa paradisiaca) on Arsenic-induced Oxidative Damage in Rat. Adv. Biochem. 2015, 3(2), 30-34. doi: 10.11648/j.ab.20150302.12

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    AMA Style

    Oluwole Israel Oyewole, Tolulope Olufunmilayo Akinbamijo, Oluwabukola Joy Omoboriowo, Naomi Ubongabasi Ukana. Hepatorenal Protective Activities of Plantain Root (Musa paradisiaca) on Arsenic-induced Oxidative Damage in Rat. Adv Biochem. 2015;3(2):30-34. doi: 10.11648/j.ab.20150302.12

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  • @article{10.11648/j.ab.20150302.12,
      author = {Oluwole Israel Oyewole and Tolulope Olufunmilayo Akinbamijo and Oluwabukola Joy Omoboriowo and Naomi Ubongabasi Ukana},
      title = {Hepatorenal Protective Activities of Plantain Root (Musa paradisiaca) on Arsenic-induced Oxidative Damage in Rat},
      journal = {Advances in Biochemistry},
      volume = {3},
      number = {2},
      pages = {30-34},
      doi = {10.11648/j.ab.20150302.12},
      url = {https://doi.org/10.11648/j.ab.20150302.12},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ab.20150302.12},
      abstract = {The protective role of aqueous root extract of Musa paradisiaca on arsenic chloride-induced oxidative damage in the liver and kidney of albino rats was investigated in this study. Twenty four albino rats were grouped in to four (A, B, C and D). Group A served as the control and received distilled water while B, C and D were administered 10 mg/kg bw of arsenic chloride weekly. Groups C and D were treated with 200 and 500 mg/kg bw of aqueous extract of Musa paradisiaca roots respectively for 28 days while group B was left untreated. Phytochemical screening carried out on the root powder indicated the presence of tannins, terpenoids, steroids, sarponins, cardiac glycosides and flavonoids. Arsenic chloride induced a significant elevation in aminotransferases (ALT and AST), ALP and total bilirubin and reduction in serum protein and albumin indicating derangement of liver function. Significant elevation of serum creatinine, urea, uric acid, blood urea nitrogen and electrolytes levels were also recorded in arsenic intoxicated rats indicating disruption of kidney function. Histological examination of the kidney and liver of arsenic intoxicated rats also indicated significant alteration in tissue architecture and morphology. There was significant increase in the liver and kidney weight index in arsenic treated groups compared to the control indicating tissue inflammation. Treatment of rats with different doses of Musa paradisiaca root extract significantly (P<0.05) normalized liver and kidney functions while it also restored normal tissue histology at the end of the experiment. It can be concluded that Musa paradisiaca contain bioactive constituents capable of protecting the living system against arsenic-induced disruption of liver and kidney functions in rats.},
     year = {2015}
    }
    

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    T1  - Hepatorenal Protective Activities of Plantain Root (Musa paradisiaca) on Arsenic-induced Oxidative Damage in Rat
    AU  - Oluwole Israel Oyewole
    AU  - Tolulope Olufunmilayo Akinbamijo
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    UR  - https://doi.org/10.11648/j.ab.20150302.12
    AB  - The protective role of aqueous root extract of Musa paradisiaca on arsenic chloride-induced oxidative damage in the liver and kidney of albino rats was investigated in this study. Twenty four albino rats were grouped in to four (A, B, C and D). Group A served as the control and received distilled water while B, C and D were administered 10 mg/kg bw of arsenic chloride weekly. Groups C and D were treated with 200 and 500 mg/kg bw of aqueous extract of Musa paradisiaca roots respectively for 28 days while group B was left untreated. Phytochemical screening carried out on the root powder indicated the presence of tannins, terpenoids, steroids, sarponins, cardiac glycosides and flavonoids. Arsenic chloride induced a significant elevation in aminotransferases (ALT and AST), ALP and total bilirubin and reduction in serum protein and albumin indicating derangement of liver function. Significant elevation of serum creatinine, urea, uric acid, blood urea nitrogen and electrolytes levels were also recorded in arsenic intoxicated rats indicating disruption of kidney function. Histological examination of the kidney and liver of arsenic intoxicated rats also indicated significant alteration in tissue architecture and morphology. There was significant increase in the liver and kidney weight index in arsenic treated groups compared to the control indicating tissue inflammation. Treatment of rats with different doses of Musa paradisiaca root extract significantly (P<0.05) normalized liver and kidney functions while it also restored normal tissue histology at the end of the experiment. It can be concluded that Musa paradisiaca contain bioactive constituents capable of protecting the living system against arsenic-induced disruption of liver and kidney functions in rats.
    VL  - 3
    IS  - 2
    ER  - 

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