Effect of Biofield Treatment on Physical, Thermal, and Spectral Properties of SFRE 199-1 Mammalian Cell Culture Medium
Advances in Biochemistry
Volume 3, Issue 6, December 2015, Pages: 77-85
Received: Oct. 8, 2015;
Accepted: Oct. 16, 2015;
Published: Nov. 16, 2015
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Mahendra Kumar Trivedi, Trivedi Global Inc., Henderson, NV, USA
Alice Branton, Trivedi Global Inc., Henderson, NV, USA
Dahryn Trivedi, Trivedi Global Inc., Henderson, NV, USA
Gopal Nayak, Trivedi Global Inc., Henderson, NV, USA
Khemraj Bairwa, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
Snehasis Jana, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
SFRE 199-1 medium (SFRE-M) is important mammalian cell culture medium, used for the culture of primary cells of mammals such as baboon kidney cells. The present study was attempted to evaluate the impact of biofield energy treatment on the physical, thermal and spectral properties of SFRE-M. The study was accomplished in two groups; one was set as control while another was subjected to Mr. Trivedi’s biofield energy treatment and coded as treated group. Subsequently, the control and treated samples were analyzed using various analytical techniques. The CHNO analysis showed about 2.16, 4.87, and 5.89% decrease in percent contents of carbon, hydrogen, and oxygen, respectively; while 9.49% increase in nitrogen contents of treated sample as compared to the control. X-ray diffraction (XRD) analysis showed 7.23% decrease in crystallite size of treated sample as compared to the control. The thermogravimetric analysis (TGA) analysis showed the increase in onset temperature of thermal degradation by 19.61% in treated sample with respect to the control. The control sample showed the 48.63% weight loss during the thermal degradation temperature (Tmax) while the treated sample showed only 13.62% weight loss during the Tmax. The differential scanning calorimetry (DSC) analysis showed the 62.58% increase in the latent heat of fusion of treated sample with respect to the control sample. The Fourier transform infrared spectroscopy (FT-IR) spectrum of treated SFRE-M showed the alteration in the wavenumber of C-O, C-N and C-H vibrations in the treated sample as compared to the control. Altogether, the XRD, TGA-DTG, DSC, and FT-IR analysis suggest that Mr. Trivedi’s biofield energy treatment has the impact on physical, thermal and spectral properties of SFRE-M. The treated SFRE-M was more thermal stable than the control SFRE-M and can be used as the better culture media for mammalian cell culture.
Mahendra Kumar Trivedi,
Effect of Biofield Treatment on Physical, Thermal, and Spectral Properties of SFRE 199-1 Mammalian Cell Culture Medium, Advances in Biochemistry.
Vol. 3, No. 6,
2015, pp. 77-85.
Taylor-Papadimitriou J, Shearer M, Watling D (1978) Growth requirements of calf lens epithelium in culture. J Cell Physiol 95: 95-103.
Mitsuhashi, J. (2012) Invertebrate tissue culture methods. Springer science & business media.
Weiss SA, Lester TL, Kalter SS, Heberling RL (1980) Chemically Defined serum-free media for the cultivation of primary cells and their susceptibility to viruses. In Vitro 16: 616-28.
Rivera-Posada J, Caballes CF, Pratchett MS (2013) Lethal doses of oxbile, peptones and thiosulfate-citrate-bile-sucrose agar (TCBS) for Acanthaster planci; exploring alternative population control options. Mar Pollut Bull 75: 133-139.
Basu S, Pal A, Desai PK (2005) Quality control of culture media in a microbiology laboratory. Ind J Med Microbiol 23: 159-163.
Ellaiah P, Srinivasulu B, Adinarayana K (2002) A review on microbial alkaline proteases. J Sci Ind Res 61: 690-704.
Trivedi MK, Patil S, Shettigar H, Singh R, Jana S (2015) An impact of biofield treatment on spectroscopic characterization of pharmaceutical compounds. Mod Chem appl 3: 159.
Trivedi MK, Nayak G, Patil S, Tallapragada RM, Jana S, Mishra RK (2015) Bio-field treatment: An effective strategy to improve the quality of beef extract and meat infusion powder. J Nutr Food Sci 5: 389.
NIH, National center for complementary and alternative medicine. CAM Basics. Publication 347. [October 2, 2008]. Available at: http://nccam.nih.gov/health/whatiscam/.
Jahn RG, Dunne BJ (1988) Margins of reality: The role of consciousness in the physical world. San Diego, CA: Harcourt Brace Jovanovich.
Rosch PJ. (2009) Bioelectromagnetic and subtle energy medicine. The interface between mind and matter. Longevity, regeneration and optimal health, New York Academy of Science.
Sances F, Flora E, Patil S, Spence A, Shinde V (2013) Impact of biofield treatment on ginseng and organic blueberry yield. Agrivita, J Agric Sci 35.
Patil SA, Nayak GB, Barve SS, Tembe RP, Khan RR (2012) Impact of biofield treatment on growth and anatomical characteristics of Pogostemon cablin (Benth.). Biotechnology 11: 154-162.
Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) An impact of biofield treatment: antimycobacterial susceptibility potential using BACTEC 460/MGIT-TB system. Mycobact Dis 5: 189.
Pavia DL, Lampman GM, Kriz GS (2001) Introduction to spectroscopy. (3rdedn), Thomson learning, Singapore.
Sato JD, Kan M (2001) UNIT 1.2 Media for culture of mammalian cells. Current protocols in cell biology.
Marquis CP. Mammalian Cell Culture. Biotechnology, 1. http://www.eolss.net/sample-chapters/c17/e6-58-01-04.pdf.
Yang Z, Xiong HR (2012) Culture conditions and types of growth media for mammalian cells.
Paiva-Santos CO, Gouveia H, Las WC, Varela JA (1999) Gauss-Lorentz size-strain broadening and cell parameters analysis of Mn doped SnO2 prepared by organic route. Mat Struct 6: 111-115.
Zhang K, Alexandrov IV, Kilmametov AR, Valiev RZ, Lu K (1997) The crystallite-size dependence of structural parameters in pure ultrafine-grained copper. J Phys D 30: 3008-3015.
Huang FY (2012) Thermal properties and thermal degradation of cellulose tri-stearate (CTs). Polymers 4: 1012-1024.
Rudnik E (2010) Compostable polymer materials. Elsevier, Oxford, UK.
Sa J (2014) Fuel production with heterogeneous catalysis. CRC Press, Taylor and Francis group LLC., FL, USA.
Trivedi MK, Patil S, Tallapragada RM (2013) Effect of bio field treatment on the physical and thermal characteristics of silicon, tin and lead powders. J Material Sci Eng 2: 125.
Smith BC (1998) Infrared spectral interpretation: A Systematic Approach. CRC Press.
Stuart BH (2004) Infrared spectroscopy: Fundamentals and applications (analytical techniques in the sciences (AnTs). John Wiley & Sons Ltd., Chichester, UK.