Transgenerational Genetic Effect of Trichloroethane (TCE) on Phenotypic Variation of Acrosomal Proteolytic Enzyme and Male Infertility Risk

Mohamed A. Al-Griw; Naser M. Salama; Soad A. Treesh; Abdul Hakim Elnfati

doi:doi:10.11648/j.ijgg.20150305.11

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Transgenerational Genetic Effect of Trichloroethane (TCE) on Phenotypic Variation of Acrosomal Proteolytic Enzyme and Male Infertility Risk

Mohamed A. Al-Griw, Naser M. Salama, Soad A. Treesh, Abdul Hakim Elnfati

Published in International Journal of Genetics and Genomics (Volume 3, Issue 5)

Received: 19 August 2015 Accepted: 31 August 2015 Published: 9 September 2015

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Abstract

Exposure to trichloroethane (TCE), a ambiguous environmental toxicant, has been negatively associated with male reproductive performance. The objective was to investigate, in-vivo, the mutagenic, carcinogenic or teratogenic effect of TCE maternal exposure on sperm quality and testicular cytoarchitecture of F1 generation of mice. A motile sperm separation technique was used to estimate sperm motility and a gelatin slide technique was used to measure the number of the halo around the acrosome of individual sperm as an acrosomal proteolytic enzyme (APA). Animals were followed up for signs of toxicity and mortality. Alterations in testicular tissues have been histopathology investigated. No adverse signs, symptoms and mortality were observed in the animals treated with TCE. Moreover, significant changes were seen in body and testis weight. Results of semen analysis revealed that TCE lead to low sperm count, abnormal sperm morphology, and frequently of sperm motility. These results were correlated with decrease in APA when pre-leptotene or spermatogonial cells were tested, indicating a transgenerational toxic effects. Histopathological examination revealed that TCE insult marked alterations in the microstructures of testicular tissues appeared as severe morphological abnormal spermatozoa and vacuoles. Taken together, these results suggest that early exposure to TCE causes testicular toxicity and poor semen quality. The sperm phenotypes utilized in this study may increase the value of sperm for detection mutagenic developmentally active agents, and agent with anti-fertility effects in mammals. This in-vivo animal model represents a unique platform for assessing human reproductive toxicity potential and genetic risk of various environmental mutagens, carcinogens and teratogens in a rapid, efficient, and unbiased format.

Published in	International Journal of Genetics and Genomics (Volume 3, Issue 5)
DOI	10.11648/j.ijgg.20150305.11
Page(s)	43-49
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Trichloroethane, Transgenerational Genetic Effect, Sperm Quality, Testicular Toxicity, Mice

References

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Mohamed A. Al-Griw, Naser M. Salama, Soad A. Treesh, Abdul Hakim Elnfati. (2015). Transgenerational Genetic Effect of Trichloroethane (TCE) on Phenotypic Variation of Acrosomal Proteolytic Enzyme and Male Infertility Risk. International Journal of Genetics and Genomics, 3(5), 43-49. https://doi.org/10.11648/j.ijgg.20150305.11

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Mohamed A. Al-Griw; Naser M. Salama; Soad A. Treesh; Abdul Hakim Elnfati. Transgenerational Genetic Effect of Trichloroethane (TCE) on Phenotypic Variation of Acrosomal Proteolytic Enzyme and Male Infertility Risk. Int. J. Genet. Genomics 2015, 3(5), 43-49. doi: 10.11648/j.ijgg.20150305.11

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AMA Style

Mohamed A. Al-Griw, Naser M. Salama, Soad A. Treesh, Abdul Hakim Elnfati. Transgenerational Genetic Effect of Trichloroethane (TCE) on Phenotypic Variation of Acrosomal Proteolytic Enzyme and Male Infertility Risk. Int J Genet Genomics. 2015;3(5):43-49. doi: 10.11648/j.ijgg.20150305.11

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@article{10.11648/j.ijgg.20150305.11,
  author = {Mohamed A. Al-Griw and Naser M. Salama and Soad A. Treesh and Abdul Hakim Elnfati},
  title = {Transgenerational Genetic Effect of Trichloroethane (TCE) on Phenotypic Variation of Acrosomal Proteolytic Enzyme and Male Infertility Risk},
  journal = {International Journal of Genetics and Genomics},
  volume = {3},
  number = {5},
  pages = {43-49},
  doi = {10.11648/j.ijgg.20150305.11},
  url = {https://doi.org/10.11648/j.ijgg.20150305.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20150305.11},
  abstract = {Exposure to trichloroethane (TCE), a ambiguous environmental toxicant, has been negatively associated with male reproductive performance. The objective was to investigate, in-vivo, the mutagenic, carcinogenic or teratogenic effect of TCE maternal exposure on sperm quality and testicular cytoarchitecture of F1 generation of mice. A motile sperm separation technique was used to estimate sperm motility and a gelatin slide technique was used to measure the number of the halo around the acrosome of individual sperm as an acrosomal proteolytic enzyme (APA). Animals were followed up for signs of toxicity and mortality. Alterations in testicular tissues have been histopathology investigated. No adverse signs, symptoms and mortality were observed in the animals treated with TCE. Moreover, significant changes were seen in body and testis weight. Results of semen analysis revealed that TCE lead to low sperm count, abnormal sperm morphology, and frequently of sperm motility. These results were correlated with decrease in APA when pre-leptotene or spermatogonial cells were tested, indicating a transgenerational toxic effects. Histopathological examination revealed that TCE insult marked alterations in the microstructures of testicular tissues appeared as severe morphological abnormal spermatozoa and vacuoles. Taken together, these results suggest that early exposure to TCE causes testicular toxicity and poor semen quality. The sperm phenotypes utilized in this study may increase the value of sperm for detection mutagenic developmentally active agents, and agent with anti-fertility effects in mammals. This in-vivo animal model represents a unique platform for assessing human reproductive toxicity potential and genetic risk of various environmental mutagens, carcinogens and teratogens in a rapid, efficient, and unbiased format.},
 year = {2015}
}

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TY - JOUR
T1 - Transgenerational Genetic Effect of Trichloroethane (TCE) on Phenotypic Variation of Acrosomal Proteolytic Enzyme and Male Infertility Risk
AU - Mohamed A. Al-Griw
AU - Naser M. Salama
AU - Soad A. Treesh
AU - Abdul Hakim Elnfati
Y1 - 2015/09/09
PY - 2015
N1 - https://doi.org/10.11648/j.ijgg.20150305.11
DO - 10.11648/j.ijgg.20150305.11
T2 - International Journal of Genetics and Genomics
JF - International Journal of Genetics and Genomics
JO - International Journal of Genetics and Genomics
SP - 43
EP - 49
PB - Science Publishing Group
SN - 2376-7359
UR - https://doi.org/10.11648/j.ijgg.20150305.11
AB - Exposure to trichloroethane (TCE), a ambiguous environmental toxicant, has been negatively associated with male reproductive performance. The objective was to investigate, in-vivo, the mutagenic, carcinogenic or teratogenic effect of TCE maternal exposure on sperm quality and testicular cytoarchitecture of F1 generation of mice. A motile sperm separation technique was used to estimate sperm motility and a gelatin slide technique was used to measure the number of the halo around the acrosome of individual sperm as an acrosomal proteolytic enzyme (APA). Animals were followed up for signs of toxicity and mortality. Alterations in testicular tissues have been histopathology investigated. No adverse signs, symptoms and mortality were observed in the animals treated with TCE. Moreover, significant changes were seen in body and testis weight. Results of semen analysis revealed that TCE lead to low sperm count, abnormal sperm morphology, and frequently of sperm motility. These results were correlated with decrease in APA when pre-leptotene or spermatogonial cells were tested, indicating a transgenerational toxic effects. Histopathological examination revealed that TCE insult marked alterations in the microstructures of testicular tissues appeared as severe morphological abnormal spermatozoa and vacuoles. Taken together, these results suggest that early exposure to TCE causes testicular toxicity and poor semen quality. The sperm phenotypes utilized in this study may increase the value of sperm for detection mutagenic developmentally active agents, and agent with anti-fertility effects in mammals. This in-vivo animal model represents a unique platform for assessing human reproductive toxicity potential and genetic risk of various environmental mutagens, carcinogens and teratogens in a rapid, efficient, and unbiased format.
VL - 3
IS - 5
ER -

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Author Information

Mohamed A. Al-Griw

Division of Developmental Biology, Zoology Department, Faculty of Science, University of Tripoli, Tripoli, Libya
Naser M. Salama

Division of Developmental Biology, Zoology Department, Faculty of Science, University of Tripoli, Tripoli, Libya
Soad A. Treesh

Department of Histology and Medical Genetics, Faculty of Medicine, University of Tripoli, Tripoli, Libya
Abdul Hakim Elnfati

Division of Developmental Biology, Zoology Department, Faculty of Science, University of Tripoli, Tripoli, Libya

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Mohamed A. Al-Griw, Naser M. Salama, Soad A. Treesh, Abdul Hakim Elnfati. (2015). Transgenerational Genetic Effect of Trichloroethane (TCE) on Phenotypic Variation of Acrosomal Proteolytic Enzyme and Male Infertility Risk. International Journal of Genetics and Genomics, 3(5), 43-49. https://doi.org/10.11648/j.ijgg.20150305.11

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Mohamed A. Al-Griw; Naser M. Salama; Soad A. Treesh; Abdul Hakim Elnfati. Transgenerational Genetic Effect of Trichloroethane (TCE) on Phenotypic Variation of Acrosomal Proteolytic Enzyme and Male Infertility Risk. Int. J. Genet. Genomics 2015, 3(5), 43-49. doi: 10.11648/j.ijgg.20150305.11

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AMA Style

Mohamed A. Al-Griw, Naser M. Salama, Soad A. Treesh, Abdul Hakim Elnfati. Transgenerational Genetic Effect of Trichloroethane (TCE) on Phenotypic Variation of Acrosomal Proteolytic Enzyme and Male Infertility Risk. Int J Genet Genomics. 2015;3(5):43-49. doi: 10.11648/j.ijgg.20150305.11

Copy | Download

@article{10.11648/j.ijgg.20150305.11,
  author = {Mohamed A. Al-Griw and Naser M. Salama and Soad A. Treesh and Abdul Hakim Elnfati},
  title = {Transgenerational Genetic Effect of Trichloroethane (TCE) on Phenotypic Variation of Acrosomal Proteolytic Enzyme and Male Infertility Risk},
  journal = {International Journal of Genetics and Genomics},
  volume = {3},
  number = {5},
  pages = {43-49},
  doi = {10.11648/j.ijgg.20150305.11},
  url = {https://doi.org/10.11648/j.ijgg.20150305.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20150305.11},
  abstract = {Exposure to trichloroethane (TCE), a ambiguous environmental toxicant, has been negatively associated with male reproductive performance. The objective was to investigate, in-vivo, the mutagenic, carcinogenic or teratogenic effect of TCE maternal exposure on sperm quality and testicular cytoarchitecture of F1 generation of mice. A motile sperm separation technique was used to estimate sperm motility and a gelatin slide technique was used to measure the number of the halo around the acrosome of individual sperm as an acrosomal proteolytic enzyme (APA). Animals were followed up for signs of toxicity and mortality. Alterations in testicular tissues have been histopathology investigated. No adverse signs, symptoms and mortality were observed in the animals treated with TCE. Moreover, significant changes were seen in body and testis weight. Results of semen analysis revealed that TCE lead to low sperm count, abnormal sperm morphology, and frequently of sperm motility. These results were correlated with decrease in APA when pre-leptotene or spermatogonial cells were tested, indicating a transgenerational toxic effects. Histopathological examination revealed that TCE insult marked alterations in the microstructures of testicular tissues appeared as severe morphological abnormal spermatozoa and vacuoles. Taken together, these results suggest that early exposure to TCE causes testicular toxicity and poor semen quality. The sperm phenotypes utilized in this study may increase the value of sperm for detection mutagenic developmentally active agents, and agent with anti-fertility effects in mammals. This in-vivo animal model represents a unique platform for assessing human reproductive toxicity potential and genetic risk of various environmental mutagens, carcinogens and teratogens in a rapid, efficient, and unbiased format.},
 year = {2015}
}

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