Molecular Characterization of Imazaquin Tolerant and Sensitive Cowpea Genotypes

Abdulrahman Lado; Muhammad Auwal Hussaini; Alpha Yaya Kamara

doi:doi:10.11648/j.ijgg.20160402.11

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Molecular Characterization of Imazaquin Tolerant and Sensitive Cowpea Genotypes

Abdulrahman Lado, Muhammad Auwal Hussaini, Alpha Yaya Kamara

Published in International Journal of Genetics and Genomics (Volume 4, Issue 2)

Received: 20 March 2016 Accepted: 1 April 2016 Published: 16 April 2016

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Abstract

In this paper, to investigate the tolerance of some cowpea genotypes to imazaquin, seeds of 30 cowpea genotypes were treated with imazaquin at 0.06 kg active ingredient ha-1 and control were treated with distilled water. Treated seeds were planted in plastic pots and raised for 3 weeks in a green house. Samples leaves were detached for DNA isolation at 2 weeks after sowing. Phenotypic result revealed that Hen-me and Hodi were the most tolerant (with 12.5% mortality rate) to imazaquin while Maptwapa and many others (with 100% mortality rate) were highly sensitive to imazaquin. However, there were no sequences differences between tolerant and sensitive genotypes in amplified region of the annotated portion of AHAS (acetohydroxy-acid synthase) from cowpea. It can be concluded that the major significant difference between imazaquin tolerance and sensitive cowpea is as a result of rapid metabolic detoxification of herbicides in tolerant cowpea. It is therefore imperative that enzymology involve in the differential metabolism of this herbicide in this crop needs urgent and necessary attention.

Published in	International Journal of Genetics and Genomics (Volume 4, Issue 2)
DOI	10.11648/j.ijgg.20160402.11
Page(s)	5-10
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

AHAS Enzyme, Imazaquin, Herbicide Tolerance and Cowpea Genotypes

References

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APA Style

Abdulrahman Lado, Muhammad Auwal Hussaini, Alpha Yaya Kamara. (2016). Molecular Characterization of Imazaquin Tolerant and Sensitive Cowpea Genotypes. International Journal of Genetics and Genomics, 4(2), 5-10. https://doi.org/10.11648/j.ijgg.20160402.11

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ACS Style

Abdulrahman Lado; Muhammad Auwal Hussaini; Alpha Yaya Kamara. Molecular Characterization of Imazaquin Tolerant and Sensitive Cowpea Genotypes. Int. J. Genet. Genomics 2016, 4(2), 5-10. doi: 10.11648/j.ijgg.20160402.11

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AMA Style

Abdulrahman Lado, Muhammad Auwal Hussaini, Alpha Yaya Kamara. Molecular Characterization of Imazaquin Tolerant and Sensitive Cowpea Genotypes. Int J Genet Genomics. 2016;4(2):5-10. doi: 10.11648/j.ijgg.20160402.11

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@article{10.11648/j.ijgg.20160402.11,
  author = {Abdulrahman Lado and Muhammad Auwal Hussaini and Alpha Yaya Kamara},
  title = {Molecular Characterization of Imazaquin Tolerant and Sensitive Cowpea Genotypes},
  journal = {International Journal of Genetics and Genomics},
  volume = {4},
  number = {2},
  pages = {5-10},
  doi = {10.11648/j.ijgg.20160402.11},
  url = {https://doi.org/10.11648/j.ijgg.20160402.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20160402.11},
  abstract = {In this paper, to investigate the tolerance of some cowpea genotypes to imazaquin, seeds of 30 cowpea genotypes were treated with imazaquin at 0.06 kg active ingredient ha-1 and control were treated with distilled water. Treated seeds were planted in plastic pots and raised for 3 weeks in a green house. Samples leaves were detached for DNA isolation at 2 weeks after sowing. Phenotypic result revealed that Hen-me and Hodi were the most tolerant (with 12.5% mortality rate) to imazaquin while Maptwapa and many others (with 100% mortality rate) were highly sensitive to imazaquin. However, there were no sequences differences between tolerant and sensitive genotypes in amplified region of the annotated portion of AHAS (acetohydroxy-acid synthase) from cowpea. It can be concluded that the major significant difference between imazaquin tolerance and sensitive cowpea is as a result of rapid metabolic detoxification of herbicides in tolerant cowpea. It is therefore imperative that enzymology involve in the differential metabolism of this herbicide in this crop needs urgent and necessary attention.},
 year = {2016}
}

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TY  - JOUR
T1  - Molecular Characterization of Imazaquin Tolerant and Sensitive Cowpea Genotypes
AU  - Abdulrahman Lado
AU  - Muhammad Auwal Hussaini
AU  - Alpha Yaya Kamara
Y1  - 2016/04/16
PY  - 2016
N1  - https://doi.org/10.11648/j.ijgg.20160402.11
DO  - 10.11648/j.ijgg.20160402.11
T2  - International Journal of Genetics and Genomics
JF  - International Journal of Genetics and Genomics
JO  - International Journal of Genetics and Genomics
SP  - 5
EP  - 10
PB  - Science Publishing Group
SN  - 2376-7359
UR  - https://doi.org/10.11648/j.ijgg.20160402.11
AB  - In this paper, to investigate the tolerance of some cowpea genotypes to imazaquin, seeds of 30 cowpea genotypes were treated with imazaquin at 0.06 kg active ingredient ha-1 and control were treated with distilled water. Treated seeds were planted in plastic pots and raised for 3 weeks in a green house. Samples leaves were detached for DNA isolation at 2 weeks after sowing. Phenotypic result revealed that Hen-me and Hodi were the most tolerant (with 12.5% mortality rate) to imazaquin while Maptwapa and many others (with 100% mortality rate) were highly sensitive to imazaquin. However, there were no sequences differences between tolerant and sensitive genotypes in amplified region of the annotated portion of AHAS (acetohydroxy-acid synthase) from cowpea. It can be concluded that the major significant difference between imazaquin tolerance and sensitive cowpea is as a result of rapid metabolic detoxification of herbicides in tolerant cowpea. It is therefore imperative that enzymology involve in the differential metabolism of this herbicide in this crop needs urgent and necessary attention.
VL  - 4
IS  - 2
ER  -

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Author Information

Abdulrahman Lado

Department of Agronomy, Bayero University, Kano, Nigeria
Muhammad Auwal Hussaini

Department of Agronomy, Bayero University, Kano, Nigeria
Alpha Yaya Kamara

International Institute of Tropical Agriculture, Kano Station, Nigeria

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Plain Text BibTeX RIS

APA Style

Abdulrahman Lado, Muhammad Auwal Hussaini, Alpha Yaya Kamara. (2016). Molecular Characterization of Imazaquin Tolerant and Sensitive Cowpea Genotypes. International Journal of Genetics and Genomics, 4(2), 5-10. https://doi.org/10.11648/j.ijgg.20160402.11

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ACS Style

Abdulrahman Lado; Muhammad Auwal Hussaini; Alpha Yaya Kamara. Molecular Characterization of Imazaquin Tolerant and Sensitive Cowpea Genotypes. Int. J. Genet. Genomics 2016, 4(2), 5-10. doi: 10.11648/j.ijgg.20160402.11

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AMA Style

Abdulrahman Lado, Muhammad Auwal Hussaini, Alpha Yaya Kamara. Molecular Characterization of Imazaquin Tolerant and Sensitive Cowpea Genotypes. Int J Genet Genomics. 2016;4(2):5-10. doi: 10.11648/j.ijgg.20160402.11

Copy | Download

@article{10.11648/j.ijgg.20160402.11,
  author = {Abdulrahman Lado and Muhammad Auwal Hussaini and Alpha Yaya Kamara},
  title = {Molecular Characterization of Imazaquin Tolerant and Sensitive Cowpea Genotypes},
  journal = {International Journal of Genetics and Genomics},
  volume = {4},
  number = {2},
  pages = {5-10},
  doi = {10.11648/j.ijgg.20160402.11},
  url = {https://doi.org/10.11648/j.ijgg.20160402.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20160402.11},
  abstract = {In this paper, to investigate the tolerance of some cowpea genotypes to imazaquin, seeds of 30 cowpea genotypes were treated with imazaquin at 0.06 kg active ingredient ha-1 and control were treated with distilled water. Treated seeds were planted in plastic pots and raised for 3 weeks in a green house. Samples leaves were detached for DNA isolation at 2 weeks after sowing. Phenotypic result revealed that Hen-me and Hodi were the most tolerant (with 12.5% mortality rate) to imazaquin while Maptwapa and many others (with 100% mortality rate) were highly sensitive to imazaquin. However, there were no sequences differences between tolerant and sensitive genotypes in amplified region of the annotated portion of AHAS (acetohydroxy-acid synthase) from cowpea. It can be concluded that the major significant difference between imazaquin tolerance and sensitive cowpea is as a result of rapid metabolic detoxification of herbicides in tolerant cowpea. It is therefore imperative that enzymology involve in the differential metabolism of this herbicide in this crop needs urgent and necessary attention.},
 year = {2016}
}

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TY  - JOUR
T1  - Molecular Characterization of Imazaquin Tolerant and Sensitive Cowpea Genotypes
AU  - Abdulrahman Lado
AU  - Muhammad Auwal Hussaini
AU  - Alpha Yaya Kamara
Y1  - 2016/04/16
PY  - 2016
N1  - https://doi.org/10.11648/j.ijgg.20160402.11
DO  - 10.11648/j.ijgg.20160402.11
T2  - International Journal of Genetics and Genomics
JF  - International Journal of Genetics and Genomics
JO  - International Journal of Genetics and Genomics
SP  - 5
EP  - 10
PB  - Science Publishing Group
SN  - 2376-7359
UR  - https://doi.org/10.11648/j.ijgg.20160402.11
AB  - In this paper, to investigate the tolerance of some cowpea genotypes to imazaquin, seeds of 30 cowpea genotypes were treated with imazaquin at 0.06 kg active ingredient ha-1 and control were treated with distilled water. Treated seeds were planted in plastic pots and raised for 3 weeks in a green house. Samples leaves were detached for DNA isolation at 2 weeks after sowing. Phenotypic result revealed that Hen-me and Hodi were the most tolerant (with 12.5% mortality rate) to imazaquin while Maptwapa and many others (with 100% mortality rate) were highly sensitive to imazaquin. However, there were no sequences differences between tolerant and sensitive genotypes in amplified region of the annotated portion of AHAS (acetohydroxy-acid synthase) from cowpea. It can be concluded that the major significant difference between imazaquin tolerance and sensitive cowpea is as a result of rapid metabolic detoxification of herbicides in tolerant cowpea. It is therefore imperative that enzymology involve in the differential metabolism of this herbicide in this crop needs urgent and necessary attention.
VL  - 4
IS  - 2
ER  -

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