American Journal of Life Sciences

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Combined Effect of Curcumin and Vitamin E against CCl4 Induced Liver Injury in Rats

Received: 23 May 2013    Accepted:     Published: 20 June 2013
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Abstract

The present study was aimed to investigate the combined effect of curcumin[CMN] and vitamin E against CCl4 induced oxidative stress and liver toxicity in rats. The toxicant CCl4 was used to induce liver toxicity at a dose of 1ml/kg as 1: 1 (v/v) mixture with liquid parafiin twice weekly for 8 weeks and sylamarinwas used as a standard drug to compare hepatoprotective effects of CMN and vitamin E. The antifibrotic effects of CMN and vitamin E were assessed directly by hepatic histology and indirectly by measuring levels of the aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP) and lipid profile (total cholesterol and triglycerides) in serum. Further, we investigated lipid peroxidation (TBARS), enzymatic antioxidants (catalase, superoxide dismutase, glutathione peroxidase, glutathione-S-transferase, glutathione reductase) and non-enzymatic antioxidant glutathione (GSH) were estimated in the liver samples. Oral administration of CMN(100mg/kg daily for 8 weeks) and in combination with vitamin E (40mg/kg thrice weekly for 8 weeks) showed significant hepatoprotection by decreasing elevated serum markers and lipid profile. In addition significantly decreased the hepatic lipid peroxidation as well as recovered the activities of antioxidant enzymes and GSH levels in the liver. Histopathological studies confirmed the biochemical observations. This study reveals CMN in combination with vitamin E possesses strong antioxidant and hepatoprotective activity than CMN alone.

DOI 10.11648/j.ajls.20130103.17
Published in American Journal of Life Sciences (Volume 1, Issue 3, June 2013)
Page(s) 117-124
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Curcumin, Carbon Tetrachloride, Oxidative Stress, Vitamin E, Silymarin

References
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Author Information
  • Department of Zoology, Sri Krishnadevaraya University, Anantapur.

  • Department of Biochemistry, Sri Krishnadevaraya University, Anantapur

  • Department of Chemistry, Sri Venkateswara University, Tirupati

  • Department of Zoology, Sri Krishnadevaraya University, Anantapur.

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    Venkatanarayana Gangarapu, Sudhakara Gujjala, Rajeswaramma Korivi, Indira Pala. (2013). Combined Effect of Curcumin and Vitamin E against CCl4 Induced Liver Injury in Rats. American Journal of Life Sciences, 1(3), 117-124. https://doi.org/10.11648/j.ajls.20130103.17

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    ACS Style

    Venkatanarayana Gangarapu; Sudhakara Gujjala; Rajeswaramma Korivi; Indira Pala. Combined Effect of Curcumin and Vitamin E against CCl4 Induced Liver Injury in Rats. Am. J. Life Sci. 2013, 1(3), 117-124. doi: 10.11648/j.ajls.20130103.17

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    AMA Style

    Venkatanarayana Gangarapu, Sudhakara Gujjala, Rajeswaramma Korivi, Indira Pala. Combined Effect of Curcumin and Vitamin E against CCl4 Induced Liver Injury in Rats. Am J Life Sci. 2013;1(3):117-124. doi: 10.11648/j.ajls.20130103.17

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  • @article{10.11648/j.ajls.20130103.17,
      author = {Venkatanarayana Gangarapu and Sudhakara Gujjala and Rajeswaramma Korivi and Indira Pala},
      title = {Combined Effect of Curcumin and Vitamin E against CCl4 Induced Liver Injury in Rats},
      journal = {American Journal of Life Sciences},
      volume = {1},
      number = {3},
      pages = {117-124},
      doi = {10.11648/j.ajls.20130103.17},
      url = {https://doi.org/10.11648/j.ajls.20130103.17},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ajls.20130103.17},
      abstract = {The present study was aimed to investigate the combined effect of curcumin[CMN] and vitamin E against CCl4 induced oxidative stress and liver toxicity in rats. The toxicant CCl4 was used to induce liver toxicity at a dose of 1ml/kg as 1: 1 (v/v) mixture with liquid parafiin twice weekly for 8 weeks and sylamarinwas used as a standard drug to compare hepatoprotective effects of CMN and vitamin E. The antifibrotic effects of CMN and vitamin E were assessed directly by hepatic histology and indirectly by measuring levels of the aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP) and lipid profile (total cholesterol and triglycerides) in serum. Further, we investigated lipid peroxidation (TBARS), enzymatic antioxidants (catalase, superoxide dismutase, glutathione peroxidase, glutathione-S-transferase, glutathione reductase) and non-enzymatic antioxidant glutathione (GSH) were estimated in the liver samples. Oral administration of  CMN(100mg/kg daily for 8 weeks) and in combination with vitamin E (40mg/kg thrice weekly for 8 weeks) showed significant hepatoprotection by decreasing elevated serum markers and lipid profile. In addition significantly decreased the hepatic lipid peroxidation as well as recovered the activities of antioxidant enzymes and GSH levels in the liver. Histopathological studies confirmed the biochemical observations. This study reveals CMN in combination with vitamin E possesses strong antioxidant and hepatoprotective activity than CMN alone.},
     year = {2013}
    }
    

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  • TY  - JOUR
    T1  - Combined Effect of Curcumin and Vitamin E against CCl4 Induced Liver Injury in Rats
    AU  - Venkatanarayana Gangarapu
    AU  - Sudhakara Gujjala
    AU  - Rajeswaramma Korivi
    AU  - Indira Pala
    Y1  - 2013/06/20
    PY  - 2013
    N1  - https://doi.org/10.11648/j.ajls.20130103.17
    DO  - 10.11648/j.ajls.20130103.17
    T2  - American Journal of Life Sciences
    JF  - American Journal of Life Sciences
    JO  - American Journal of Life Sciences
    SP  - 117
    EP  - 124
    PB  - Science Publishing Group
    SN  - 2328-5737
    UR  - https://doi.org/10.11648/j.ajls.20130103.17
    AB  - The present study was aimed to investigate the combined effect of curcumin[CMN] and vitamin E against CCl4 induced oxidative stress and liver toxicity in rats. The toxicant CCl4 was used to induce liver toxicity at a dose of 1ml/kg as 1: 1 (v/v) mixture with liquid parafiin twice weekly for 8 weeks and sylamarinwas used as a standard drug to compare hepatoprotective effects of CMN and vitamin E. The antifibrotic effects of CMN and vitamin E were assessed directly by hepatic histology and indirectly by measuring levels of the aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP) and lipid profile (total cholesterol and triglycerides) in serum. Further, we investigated lipid peroxidation (TBARS), enzymatic antioxidants (catalase, superoxide dismutase, glutathione peroxidase, glutathione-S-transferase, glutathione reductase) and non-enzymatic antioxidant glutathione (GSH) were estimated in the liver samples. Oral administration of  CMN(100mg/kg daily for 8 weeks) and in combination with vitamin E (40mg/kg thrice weekly for 8 weeks) showed significant hepatoprotection by decreasing elevated serum markers and lipid profile. In addition significantly decreased the hepatic lipid peroxidation as well as recovered the activities of antioxidant enzymes and GSH levels in the liver. Histopathological studies confirmed the biochemical observations. This study reveals CMN in combination with vitamin E possesses strong antioxidant and hepatoprotective activity than CMN alone.
    VL  - 1
    IS  - 3
    ER  - 

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