American Journal of Life Sciences

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Phenotypic Diversity and Molecular Identification of the Most Prevalent Anastomosis Group of Rhizoctonia solani Isolated from Diseased Faba Bean Plants

Received: 31 December 2014    Accepted: 18 January 2015    Published: 13 February 2015
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Abstract

One hundred and thirty one isolates of Rhizoctonia spp. were isolated from faba bean plants showing root rot and stem canker collected from different fields in Delta region of Egypt. Forty six isolates were found to be polynucleate and the remaining isolates were binucleates. According to morphological features of isolates, they were classified into 12 groups. Polynucleate isolates were identified as Rhizoctonia solani (Kühn). The most aggressive isolate of R. solani was identified according to sequences of ITS1-5.8S rDNA-ITS4 and the sequence was compared with Thanatephorus cucumeris (teleomorphic phase) and other R. solani (NCBI GenBank). Sequence and comparison revealed that this isolate is R. solani AG4-HGI. Anastomosis test carried out between molecular identified isolate and 11 randomly chosen isolates resembles all groups of polynucleate R. solani. All tested isolates were completely fused between each other indicating that the prevalent AG of R. solani on faba bean is AG4-HG1.

DOI 10.11648/j.ajls.20150301.19
Published in American Journal of Life Sciences (Volume 3, Issue 1, February 2015)
Page(s) 47-55
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This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Anastmosis Group, Rhizoctonia Solani AG4-HGI, PCR, Grouping of isolates, Phylogenetic Analysis

References
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[4] Fenille, R. C.; Ciampi, M. B.; Kuramae, E. E. and Souza, N. L. (2003). Identification of Rhizoctonia solani Associated with Soybean in Brazil by rDNA-ITS Sequences. Fitopatologia Brasileira 28:413-419. 2003.
[5] Gonzalez, D.; Carling, D. E.; Kuninaga, S.; Vilgalys, R. And Cubeta, M. A. (2001). Rhibosomal DNA systematics of Ceratobasidium and Thanatephorus with Rhizoctonia anamorphs. Mycologia., 93: 1138-1150.
[6] Kim, H. T.; Chung, Y. R. and Cho, K. Y. (2001). Mycelial melanization of Rhizoctonia solani AG1 affected pathogenicity in rice. Plant Patho. J., 17 (4): 210-215.
[7] Kuninaga, S. and Yokosawa, R. (1982a). DNA based sequence homology in Rhizoctonia solani Kühn. I. Genetic relatedness within anastomosis group 1. Annals of the Phytopathological Society of Japan 48:659-667.
[8] Kuninaga, S. and Yokosawa, R. (1982b). DNA based sequence homology in Rhizoctonia solani Kühn. II. Genetic relatedness within anastomosis group 2. Annals of the Phytopathological Society of Japan 48:668-673.
[9] Kuninaga, S. and Yokosawa, R. (1984). DNA base-sequence homology in Rhizoctonia solani Kühn. IV. Genetic relatedness within AG-4. Ann Phytopathol Soc 50:322-330.
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[11] Kuninaga, S; Natsuaki, T.; Takeuchi, T. and Yokosawa, R. (1997). Sequence variation of the rDNA ITS regions within and between anastomosis groups in Rhizoctonia solani. Current Genetics., 32: 237-243.
[12] Kuramae-Izioka, E.E. (1997). A rapid. easy and high yield protocol for total genomic DNA isolation from Colletotrichum gloeosporioides and Fusarium oxysporum for RAPD. Revista Unimar 19:683-689.
[13] Lamari, L. and Bernier, C. C. (1985). Etiology of seedling blight and root rot of faba bean (Vicia faba) in Manitoba. Can. J. Plant Pathol., 7: 139-145.
[14] Maha, H. Mohamed; Gado, E. A. M.; El-Deeb, S. H. and Mostafa, M. H. (2014). Behavior of fungus Rhizoctonia solani under faba bean cotyledons when away from host and the effect of its starvation on aggressiveness. Journal of Yeast and Fungal Research 5(1): 1-8.
[15] Omar, S. A. M. (1986). Pathological studies on root rot diseases of faba bean (Vicia faba). Faba Bean Information Service (FABIS) Newsletter., 14: 34-37.
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[17] Saitou,N., and Nei, M. (1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4 (4): 406-425.
[18] Salt, G. A. (1982). Factors affecting resistance to root rot and wilt diseaes. Pages 259-267 in G. C. Hawtin and C. Webb, eds., Faba Bean Improvement. Martinus Nijhoff Publishers, The Netherlands.
[19] Sneh, B.; Burpee, L. and Ogoshi, A. (1991). Identification of Rhizoctonia Species. St Paul, MN, APS Press, pp. 133.
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Author Information
  • Plant Pathology Department, Faculty of Agriculture, Ain Shams University, Cairo, Egypt

  • Biology Department, Faculty of Science, Taif University, Taif, Saudi Arabia

  • Plant Pathology Department, Faculty of Agriculture, Ain Shams University, Cairo, Egypt

  • Plant Pathology Department, Faculty of Agriculture, Ain Shams University, Cairo, Egypt

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  • APA Style

    Mohamed Maha Helmy, Gado Emad, El-Deeb Samir, Mostafa Helmy Mostafa. (2015). Phenotypic Diversity and Molecular Identification of the Most Prevalent Anastomosis Group of Rhizoctonia solani Isolated from Diseased Faba Bean Plants. American Journal of Life Sciences, 3(1), 47-55. https://doi.org/10.11648/j.ajls.20150301.19

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    ACS Style

    Mohamed Maha Helmy; Gado Emad; El-Deeb Samir; Mostafa Helmy Mostafa. Phenotypic Diversity and Molecular Identification of the Most Prevalent Anastomosis Group of Rhizoctonia solani Isolated from Diseased Faba Bean Plants. Am. J. Life Sci. 2015, 3(1), 47-55. doi: 10.11648/j.ajls.20150301.19

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    AMA Style

    Mohamed Maha Helmy, Gado Emad, El-Deeb Samir, Mostafa Helmy Mostafa. Phenotypic Diversity and Molecular Identification of the Most Prevalent Anastomosis Group of Rhizoctonia solani Isolated from Diseased Faba Bean Plants. Am J Life Sci. 2015;3(1):47-55. doi: 10.11648/j.ajls.20150301.19

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  • @article{10.11648/j.ajls.20150301.19,
      author = {Mohamed Maha Helmy and Gado Emad and El-Deeb Samir and Mostafa Helmy Mostafa},
      title = {Phenotypic Diversity and Molecular Identification of the Most Prevalent Anastomosis Group of Rhizoctonia solani Isolated from Diseased Faba Bean Plants},
      journal = {American Journal of Life Sciences},
      volume = {3},
      number = {1},
      pages = {47-55},
      doi = {10.11648/j.ajls.20150301.19},
      url = {https://doi.org/10.11648/j.ajls.20150301.19},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ajls.20150301.19},
      abstract = {One hundred and thirty one isolates of Rhizoctonia spp. were isolated from faba bean plants showing root rot and stem canker collected from different fields in Delta region of Egypt. Forty six isolates were found to be polynucleate and the remaining isolates were binucleates. According to morphological features of isolates, they were classified into 12 groups. Polynucleate isolates were identified as Rhizoctonia solani (Kühn). The most aggressive isolate of R. solani was identified according to sequences of ITS1-5.8S rDNA-ITS4 and the sequence was compared with Thanatephorus cucumeris (teleomorphic phase) and other R. solani (NCBI GenBank). Sequence and comparison revealed that this isolate is R. solani AG4-HGI. Anastomosis test carried out between molecular identified isolate and 11 randomly chosen isolates resembles all groups of polynucleate R. solani. All tested isolates were completely fused between each other indicating that the prevalent AG of R. solani on faba bean is AG4-HG1.},
     year = {2015}
    }
    

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    AU  - Mohamed Maha Helmy
    AU  - Gado Emad
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    AB  - One hundred and thirty one isolates of Rhizoctonia spp. were isolated from faba bean plants showing root rot and stem canker collected from different fields in Delta region of Egypt. Forty six isolates were found to be polynucleate and the remaining isolates were binucleates. According to morphological features of isolates, they were classified into 12 groups. Polynucleate isolates were identified as Rhizoctonia solani (Kühn). The most aggressive isolate of R. solani was identified according to sequences of ITS1-5.8S rDNA-ITS4 and the sequence was compared with Thanatephorus cucumeris (teleomorphic phase) and other R. solani (NCBI GenBank). Sequence and comparison revealed that this isolate is R. solani AG4-HGI. Anastomosis test carried out between molecular identified isolate and 11 randomly chosen isolates resembles all groups of polynucleate R. solani. All tested isolates were completely fused between each other indicating that the prevalent AG of R. solani on faba bean is AG4-HG1.
    VL  - 3
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