The aim of this paper is isolating rare actinobacteria from new ecological source as corrosive lesion at concrete infrastructure and screening their ability to produce biological products. Ten pure actinobacteria isolates were isolated from corrosive lesions at concrete infrastructures of irrigation channel using cement extract media at different pHs (9- 12) and incubated at 30°C for 7d. All of the isolates produced variable levels of cellulase and lipase, and nine of them displayed variable levels of alkaline protease and amylase products. Only Four isolates produced extracellular alkaline phosphatase in liquid media. The antagonistic activities of these isolates were screened against four pathogenic microorganisms including Gram positive and negative bacterial species and two species of fungi. Only the isolate ROR40 exhibited antagonism activity against Staphylococcus aureus and Escherichia coli , whereas nine isolates showed different degrees of antagonism activities against Microsporum canis and Trichophyton mentagrophyte. The potential isolate (ROR40) to produce extracellular alkaline phosphatase was selected and identified depending on phenotypical, physiological and molecular according to partial sequences of 16S r RNA gene. It had 98% similarity with Pseudonocardia alni 20049 and P. alni 44104. Finally the isolate is named Pseudonocardia sp. ROR40 (Genbank accession no KJ 725072). The isolate produced extracellular alkaline phosphatase in liquid medium at optimum conditions were pH 8.5 of production medium, 37 °C for 4d. in stand incubator. Conclusion: Our study would be the first instance in comprehensive characterization of concert deteriorating actinobacteria for producing commercially valuable primary and secondary metabolites and it may facilitate us to isolate and characterize more bioactive species.
| Published in | American Journal of Life Sciences (Volume 3, Issue 4) |
| DOI | 10.11648/j.ajls.20150304.11 |
| Page(s) | 247-256 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Concrete Corrosive Lesions, Actinobacteria, Secondary Metabolites, Pseudonocardia, Alkaline Phosphatase
| [1] | Ara, I., Tsetseg, B., Daram, D., Suto, M. and Ando, K. (2011). Pseudonocardia mongoliensis sp. nov. and Pseudonocardia khuvsgulensis sp. nov., isolated from soil. IJSEM, 61 ( 4): 747-756 |
| [2] | Baranov, K., Volkovam, O., Chikaev, N., Mechetina, L., Laktionov, P., Najakshin, A. and Taranin, A. (2008) A direct antigen-binding assay for detection of antibodies against native epitopes using alkaline phosphatase-tagged proteins. J Immunol, Meth. 332:73- 81. |
| [3] | Berdy, J. (2005) Bioactive microbial metabolites. J Antibiot Tokyo, 58:1–26 |
| [4] | Biehle, J.R., Cavalieri, S.J., Felland, T., Zimmer, B.L.(1996) Novel method for rapid identification of Nocardiaspecies by detection of performed enzymes. J Clinic Microbiol, 34:103–107. |
| [5] | Bradford, M.(1976) A rapid and sensitive method for the quantitation of migrogram quintities of protein using the principle of protein-dye binding. Ana Biochem, 72: 248-254. |
| [6] | Crawford, D.L., Lynch, J.M., Whipps, J.M., Ousley, M.A. (1993) Isolation and characterization of actinomycete antagonists of a fungal root pathogen. Appl Environ Microbiol, 59:3899–3905. |
| [7] | Dhaked, R.K., Alam, S.I., Dixit, A. and Singh, L. (2005) Purification and characterization of thermo-labile alkaline phosphatase from an Antarctic psychrotolerant Bacillus sp. P9. Enzyme and Microbial Technol, 36 (7): 855-861. |
| [8] | Franco-Correaa,M., Quintanaa, A., Duquea, C., Suarez, C., Rodríguez , M. X., Barea , J.M. (2010) Evaluation of actinomycete strains for key traits related with plant growth promotion and mycorrhiza helping activities. Appl Soil Ecol, 45 209–217. |
| [9] | Gandhimathi, R., Kiran G. S., Hema, T.A., Selvin, J., Rajeetha, R. and Shanmughapriya, S. (2009) Production and characterization of lipopeptide biosurfactant by a sponge-associated marine actinomycetes Nocardiopsis alba MSA10. Bioprocess Biosyst Eng , 32:825-835. |
| [10] | Ghorbani-Nasrabadi, R., Greiner, R., Alikhani, H. A. and Hamedi, J. (2012). Identification and determination of extracellular phytate-degrading activity in actinomycetes. World J Microbiol Biotechnol,28: 2601–2608 |
| [11] | Goodfellow, M. (2012) Phylum XXVI Actinobacteria phyl. nov. In Bergey’s Manual of Systematic Bacteriology 2nd edition. M. Goodfellow ,P. Kampfer ,H.J. Busse,M.E. Tru-jillo ,K. Suzuki, W. Ludwig (eds.), Whitman. pp.33-34 |
| [12] | Hamdali, H., Bouizgarne, B., Hafidi, M., Lebrihi, A., Virolle, M.J. and Ouhdouch, Y. (2008) Screening for rock phosphate solubilizing Actinomycetes from Moroccan phosphate mines. Appl Soil Ecol, 38: 12-19. |
| [13] | Khazal, M.J. (2013) Isolation and molecular identification of some microbial communities fouling concrete infrastructures. Ph.D. thesis . Babylon University. |
| [14] | Leon, J., Liza, L., Soto, I., Cuadra, D., Patino, L. and Zerpa, R. (2007) Bioactives actinomycetes of marine sediment from the central coast of Peru. Revi Peru Boil, 14:259–270. |
| [15] | Luo, H.Y., Wang, Y.R., Miao, L.H., Yang, P.L., Shi, P.J., Fang, C.X., Yao, B. and Fan, Y.L. (2009) Nesterenkonia albasp. nov., an alkaliphilic actinobacterium isolated from the black liquor treatment system of a cotton pulp mill. Int J Syst Evol Microbiol, 59:863-868. |
| [16] | Madigan, M. T., Martinko, J.M., Stahl, D.A. and Clark, D.P. (2012) Commercial Products and Biotechnology. In Brock biology of microorganisms. 13th ed. Benjamin cumings.pp. 415-416. |
| [17] | Meena, B., Rajan, L.A., Vinithkumar, N.V. and Kirubagaran, R. (2013) Novel marine actinobacteria from emerald Andaman & Nicobar Islands: a prospective source for industrial and pharmaceutical byproducts. BMC Microbiol, 22; 13:145. |
| [18] | Moura ,R. S., Martı́n, J.F., Martı́n , A. and Liras , P. (2001) Substrate analysis and molecular cloning of the extracellular alkaline phosphatase of Streptomyces griseus. Microbiology, 147 (6):1525-1533. |
| [19] | Omran, R. and Qaddoori, J.A. ((2014) Optimize environmental production conditions of extracellular alkaline phosphatase from Bacillus sp. I. WJPR, 3(8): 1-11. |
| [20] | Pandey, S.K. and Banik, R.M. (2010) Optimization of process parameters for alkaline phosphatase production by Bacillus licheniformis using response surface methodology. J Agri Technol, 6(4):721-732. |
| [21] | Ramesh, S. and Mathivanan, N. (2009) Screening of marine actinomycetes isolated from the Bay of Bengal, India for antimicrobial activity and industrial enzymes. World J Microbiol Biotechnol, 25:2103-2111. |
| [22] | Reysenbach, A.L.; Wickham, G.S. and Pace, N.R. (1994). Phylogenetic analysis of the hyperthermophilic pink filament community in Octopus Spring, Yellowstone National Park. Appl Environ Microbiol, 60: 2113-2119. |
| [23] | Richardson, A.E., Barea, J.M., McNeill, A.M., Prigent-Combaret, C. (2009) Acquisition of phosphorus and nitrogen in the rhizosphere and plant growth promotion by microorganisms. Plant Soil 321, 305–339. |
| [24] | Rosenberg, E. and Ron, E.Z. (2001) Natural roles of biosurfactants. Environ Microbiol, 3:229- 236. |
| [25] | Rudi,K., Skulberg, O.M., Larsen, F. and Jacoksen, K.S. (1997) Strain classification of oxyphotobacteria in clone culture on the bases of 16S rRNA sequences from variable regions V6, V7 and V8. App Environ Microbiol, 63: 2593-2599. |
| [26] | Saitou, N. and Nei, M. (1987) The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol Biol Evol, 4:406-425. |
| [27] | Shirling, E.B. and Gottileb, D. (1966) Methods for characterization of Streptomyces species. Int J Syst Bactriol , 16:312-340. |
| [28] | Sigrid, H., Espen, F., Kjell, D.J., Elena, I., Trond, E.E. and Sergey, B.Z (2008) Characterization of streptomyces spp. Isolated from the Sea surface microlayer in the Trondheim fjord, Norway. Mar Drugs, 6:620–635. |
| [29] | Singh, P., Thumar, J.T., Gohel, S.D. and Purohit, M.K. (2010) Molecular diversity and enzymatic potential of salt-tolerent alkaliphilic actinomycetes. In Curr Res Technol Education Topics in Appl Microbiol Microbial Biotechnol Edited by Mendez A. |
| [30] | Sultana, G.N.N. and Khan, A.H. (2007) Optimization of the sample preparation method for DNA sequencing. J Biol Sci, 7(1): 194-199. |
| [31] | Takizawa, M, Colwell, R.R and Hill, R.T. (1993) Isolation and diversity of actinomycetes in the Chesapeake Bay. Appl Environ Microbiol, 59:997-1002. |
| [32] | Tamura, K., Nei, M., and Kumar, S. (2004) Prospects for inferring very large phylogenies by using the neighbor-joining method. Proceedings of the National Academy of Sciences (USA) 101:11030-11035. |
| [33] | Tamura, K., Stecher, G., Peterson, D., Filipski, A., and Kumar, S. (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol, 30: 2725-2729. |
| [34] | Vasconcellos, R.L.F., Silva, M.C.P., Ribeiro, C.M. and Cardos, E.J.B.N. (2010) Isolation and screening for plant growth-promoting (PGP) actinobacteria from Araucaria angustifolia rhizosphere soil. Sci agric (Piracicaba, Braz.) [online], 67 (6): 743-746 |
| [35] | Wright, E.S., Yilmaz, L.S. and Noguera, D.R.(2012) DECIPHER, a Search- Based Approach to Chimera identification for 16S rRNA sequences. Appl. Environ. Microbiol., 78:717-725. |
| [36] | Zhang, Z., Schwartz, S., Wagner, L. and Miller, W. (2000) A greedy algorithm for aligning DNA sequences. J Comput Biol, 7(1-2):203-14. |
APA Style
Rabab Omran. (2015). Corrosive Lesions at Concrete Infrastructures as Promising Source for Isolating Bioactive Actinobacteria. American Journal of Life Sciences, 3(4), 247-256. https://doi.org/10.11648/j.ajls.20150304.11
ACS Style
Rabab Omran. Corrosive Lesions at Concrete Infrastructures as Promising Source for Isolating Bioactive Actinobacteria. Am. J. Life Sci. 2015, 3(4), 247-256. doi: 10.11648/j.ajls.20150304.11
AMA Style
Rabab Omran. Corrosive Lesions at Concrete Infrastructures as Promising Source for Isolating Bioactive Actinobacteria. Am J Life Sci. 2015;3(4):247-256. doi: 10.11648/j.ajls.20150304.11
Share This Article
Twitter
Linked In
Facebook
Copy
Download@article{10.11648/j.ajls.20150304.11,
author = {Rabab Omran},
title = {Corrosive Lesions at Concrete Infrastructures as Promising Source for Isolating Bioactive Actinobacteria},
journal = {American Journal of Life Sciences},
volume = {3},
number = {4},
pages = {247-256},
doi = {10.11648/j.ajls.20150304.11},
url = {https://doi.org/10.11648/j.ajls.20150304.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20150304.11},
abstract = {The aim of this paper is isolating rare actinobacteria from new ecological source as corrosive lesion at concrete infrastructure and screening their ability to produce biological products. Ten pure actinobacteria isolates were isolated from corrosive lesions at concrete infrastructures of irrigation channel using cement extract media at different pHs (9- 12) and incubated at 30°C for 7d. All of the isolates produced variable levels of cellulase and lipase, and nine of them displayed variable levels of alkaline protease and amylase products. Only Four isolates produced extracellular alkaline phosphatase in liquid media. The antagonistic activities of these isolates were screened against four pathogenic microorganisms including Gram positive and negative bacterial species and two species of fungi. Only the isolate ROR40 exhibited antagonism activity against Staphylococcus aureus and Escherichia coli , whereas nine isolates showed different degrees of antagonism activities against Microsporum canis and Trichophyton mentagrophyte. The potential isolate (ROR40) to produce extracellular alkaline phosphatase was selected and identified depending on phenotypical, physiological and molecular according to partial sequences of 16S r RNA gene. It had 98% similarity with Pseudonocardia alni 20049 and P. alni 44104. Finally the isolate is named Pseudonocardia sp. ROR40 (Genbank accession no KJ 725072). The isolate produced extracellular alkaline phosphatase in liquid medium at optimum conditions were pH 8.5 of production medium, 37 °C for 4d. in stand incubator. Conclusion: Our study would be the first instance in comprehensive characterization of concert deteriorating actinobacteria for producing commercially valuable primary and secondary metabolites and it may facilitate us to isolate and characterize more bioactive species.},
year = {2015}
}
TY - JOUR T1 - Corrosive Lesions at Concrete Infrastructures as Promising Source for Isolating Bioactive Actinobacteria AU - Rabab Omran Y1 - 2015/06/17 PY - 2015 N1 - https://doi.org/10.11648/j.ajls.20150304.11 DO - 10.11648/j.ajls.20150304.11 T2 - American Journal of Life Sciences JF - American Journal of Life Sciences JO - American Journal of Life Sciences SP - 247 EP - 256 PB - Science Publishing Group SN - 2328-5737 UR - https://doi.org/10.11648/j.ajls.20150304.11 AB - The aim of this paper is isolating rare actinobacteria from new ecological source as corrosive lesion at concrete infrastructure and screening their ability to produce biological products. Ten pure actinobacteria isolates were isolated from corrosive lesions at concrete infrastructures of irrigation channel using cement extract media at different pHs (9- 12) and incubated at 30°C for 7d. All of the isolates produced variable levels of cellulase and lipase, and nine of them displayed variable levels of alkaline protease and amylase products. Only Four isolates produced extracellular alkaline phosphatase in liquid media. The antagonistic activities of these isolates were screened against four pathogenic microorganisms including Gram positive and negative bacterial species and two species of fungi. Only the isolate ROR40 exhibited antagonism activity against Staphylococcus aureus and Escherichia coli , whereas nine isolates showed different degrees of antagonism activities against Microsporum canis and Trichophyton mentagrophyte. The potential isolate (ROR40) to produce extracellular alkaline phosphatase was selected and identified depending on phenotypical, physiological and molecular according to partial sequences of 16S r RNA gene. It had 98% similarity with Pseudonocardia alni 20049 and P. alni 44104. Finally the isolate is named Pseudonocardia sp. ROR40 (Genbank accession no KJ 725072). The isolate produced extracellular alkaline phosphatase in liquid medium at optimum conditions were pH 8.5 of production medium, 37 °C for 4d. in stand incubator. Conclusion: Our study would be the first instance in comprehensive characterization of concert deteriorating actinobacteria for producing commercially valuable primary and secondary metabolites and it may facilitate us to isolate and characterize more bioactive species. VL - 3 IS - 4 ER -
Information
Email: