Antibacterial Activity of Mentha pulegium L. from Turkey
American Journal of Life Sciences
Volume 3, Issue 6, December 2015, Pages: 383-386
Received: Oct. 21, 2015; Accepted: Oct. 29, 2015; Published: Dec. 2, 2015
Views 3695      Downloads 84
Authors
Murat Aycan, Graduate School of Natural and Applied Sciences, Ankara University, Ankara, Turkey
Mustafa Yildiz, Department of Field Crops, Ankara University, Ankara, Turkey
Selcen Darcin, Department of Field Crops, Bilecik Şeyh Edebali University, Bilecik, Turkey
Kenan Tunc, Department of Biology, Sakarya University, Sakarya, Turkey
Aysegul Hos, Department of Biology, Sakarya University, Sakarya, Turkey
Elif Dundar, Graduate School of Health Sciences, Anadolu University, Eskişehir, Turkey
Article Tools
Follow on us
Abstract
In this research, the antibacterial activity of crude extracts obtained from leaves of pennyroyal (Mentha pulegium L.) plant against Escherichia coli ATCC 25922, Enterococcus faecalis ATCC 29212, Staphylococcus aureus ATCC 29213 and Staphylococcus epidermidis ATCC 12228 was investigated. Acetone and methanol were used as chemical solvents to prepare the extracts of Mentha pulegium L. The determination of antibacterial activity was performed by using disc diffusion method. For each extract, its own solvent was utilized as negative control. Standard antibiotic discs (Clindamycin, Tetracycline and Amoxicillin-Clavulanic acid) were also used as positive control. The results of present study showed that the crude extracts of Mentha pulegium L. prepared in acetone and methanol had antibacterial activity against test microorganisms and the most antibacterial effect was observed against Staphylococcus epidermidis. It was determined that the extract of Mentha pulegium L. (6400 µg/disc) has more antibacterial activity against E. faecalis and S. epidermidis than Tetracycline (10 µg/disc) and has more antibacterial activity against E. coli than Clindamycin (10 µg/disc). However it was found that antibacterial activity of Mentha pulegium L. extract (6400 µg/disc) was close to Tetracycline (10 µg/disc) for E. coli and S. aureus and was close to Clindamycin (10 µg/disc) for E. faecalis.
Keywords
Antibacterial Activity, Mentha pulegium L., Pennyroyal
To cite this article
Murat Aycan, Mustafa Yildiz, Selcen Darcin, Kenan Tunc, Aysegul Hos, Elif Dundar, Antibacterial Activity of Mentha pulegium L. from Turkey, American Journal of Life Sciences. Vol. 3, No. 6, 2015, pp. 383-386. doi: 10.11648/j.ajls.20150306.11
Copyright
Copyright © 2015 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
References
[1]
A. Zainab, R. Bhat P, S. Acharya, A. Yende, P. S. Prajna, S. Padyana. Studies on Antioxidant and Antimicrobial Activities of Pajanelia longifolia (Willd.) Schumann. Journal of Research in Obesity. 2013. pp. 1-12.
[2]
A. Tajehmiri, F. Issapour, M. N. Moslem, M. T. Lakeh, M. H. Kolavani. In vitro Antimicrobial Activity of Artemisia annua Leaf Extracts against Pathogenic Bacteria. Advanced Studies in Biology. 2014. pp. 93-97.
[3]
K. Tunç, A. Hoş, B. Güneş. Investigation of antibacterial properties of Cotinus coggygria from Turkey. Pol. J. Environ. Stud. 2013. pp. 1559-1561.
[4]
K. Singh, V. Tiwari, R. Prajapat. Study of antimicrobial activity of medicinal plants against various multiple drug resistance pathogens and their molecular characterization and it’s bioinformatics analysis of antibiotic gene from genomic database with degenerate primer prediction. International Journal of Biological Technology. 2010. pp. 15-19.
[5]
M. Seladji, N. Belmekki, C. Bekhechi, N. Bendimerad. Antioxidant and antimicrobial activity of aqueous and methanolic extracts of Mentha rotundifolia L. from Algeria. Int. J. Pharm. Sci. Rev. Res. 2014. pp. 228-234.
[6]
L. E. Graham, J. M. Graham, L. W. Wilcox. Plant biology (1st ed) Prentice Hall, inc. in: K. Işık (Ed), Palme Yayıncılık. 2008.
[7]
H. Ghazghazi, A. Chedia, M. Weslati, F. Trakhna, S. Houssine, M. Abderrazak, H. Brahim. Chemical composition and in vitro antimicrobial activities of Mentha pulegium leaves extracts against foodborne pathogens. Journal of Food Safety. 2013. pp. 239–246.
[8]
T. Baytop. Therapy with medicinal plants in Turkey (2nd ed.) Nobel Tıp Kitabevleri. 1999.
[9]
M. C. T. Duarte, G. M. Figueira, A. Sartoratto, V. L. G. Rehder, C. Delarmelina. Anti-Candida activity of Brazilian medicinal plants. Journal of Ethnopharmacology. 2005. pp. 305–311.
[10]
P. M. G. Soares, A. M. S. Assreuy, E. P. Souza, R. F. Lima, T. O. Silva, S. R. Fontenele, D. N. Criddle. Inhibitory effects of the essential oil of Mentha pulegium on the isolated rat myometrium. Planta Med. 2005. pp. 214-218.
[11]
H. Jouad, M. Haloui, H. Rhiouani, J. El Hilaly, M. Eddouks. Ethnobotanical survey of medicinal plants used for the treatment of diabetes, cardiac and renal diseases in the North centre region of Morocco (Fez–Boulemane). Journal of Ethnopharmacology. 2001. pp. 175–182.
[12]
E. Tekin. The most beautiful wild flowers of Turkey (Vol. 1). Türkiye İş Bankası Kültür Yayınları. 2007.
[13]
P. H. Davis. Flora of Turkey and the east Aegean islands. Great Britain:Edinburgh University Press. Vol 7, 1982.
[14]
E. Tuzlacı. Türkiye’nin yabani besin bitkileri ve ot yemekleri. Alfa Basım Yayım Dağıtım Ltd. 2011.
[15]
B. Marzouk, M. B. H. Fredj, I. Chraief, M. Mastouri, K. Boukef, Z. Marzouk. Chemical composition and antimicrobial activity of essential oils from Tunisian Mentha pulegium L. Journal of Food Agriculture & Environment. 2008. pp. 78-82.
[16]
H. Boukhebti, A. N. Chaker, H. Belhadj, F. Sahli, M. Ramdhani, H. Laouer, D. Harzallah. Chemical composition and antibacterial activity of Mentha pulegium L. and Mentha spicata L. essential oils. Der Pharmacia Lettre. 2011. pp. 267-275.
[17]
F. H. Shirazi, N. Ahmadi, M. Kamalinejad. Evaluation of northern Iran Mentha pulegium L. cytotoxicity. DARU. 2004. pp. 106-110.
[18]
A. Sivropoulou, S. Kokkini, T. Lanaras, M. Arsenakis. Antimicrobial activity of mint essential oils. J Agric. Food Chem. 1995. pp. 2384-2388.
[19]
M. Mahboubi, G. Haghi. Antimicrobial activity and chemical composition of Mentha pulegium L. essential oil. Journal of Ethnopharmacology. 2008. pp. 325–327.
[20]
A. Stanojkovic´, J. Cekovic´, R. Pivic´, S. Maksimovic´. Antibacterial activity of Mentha piperita and Mentha pulegium extracts. Proceedings of the Fifth Conference on Medicinal and Aromatic Plants of Southeast European Countries, 5th CMAPSEEC. 2008.
ADDRESS
Science Publishing Group
1 Rockefeller Plaza,
10th and 11th Floors,
New York, NY 10020
U.S.A.
Tel: (001)347-983-5186