American Journal of Life Sciences
Volume 3, Issue 6, December 2015, Pages: 395-401
Received: Sep. 29, 2015;
Accepted: Oct. 19, 2015;
Published: Dec. 22, 2015
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Peter A. I., Department of Anatomy, University of Uyo, Uyo, Nigeria
Azu O. O., Department of Anatomy, University of Kwa-Zulu Natal, Durban, South Africa
Edagha I. A., Department of Anatomy, University of Uyo, Uyo, Nigeria
This study was designed to investigate the cytoprotective effects of ethanolic extract of Vernonium amygadlina leaves on Alloxan induced hepato-toxicity in albino Wistar rats. A total of forty (45) male Wister Albino rats weighing between 130g to 200g were obtained from the animal house of the Faculty of Basic Medical Sciences, University of Calabar. The animals were randomly divided into nine groups of five rats each after acclimatization in the animal house of the College of Health Sciences, University of Uyo for two weeks. Group A served as control and were administered 1 ml of distil water, Group B and C were administered with 150 mg/kg and 300mg/kg of Vernonia amygdalina respectively. Group D was administered with 65mg/kg of Alloxan. Group E and F were injected intraperitonially with 65mg/kg of Alloxan and left for two weeks to become diabetic followed by administration with 150mg/kg and 300mg/kg of Vernonia amygdalina respectively. Group G and H were administered with 150mg/kg and 300 mg/kg of Vernonia amygdalina respectively for two weeks, before they were injected intraperitonially with 65mg/kg of Alloxan followed by continuation of 150mg/kg and 300mg/kg of Vernonia amygdalina for another two weeks. Group I was administered with 65mg/kg of Alloxan and insulin lente (1.0 IU) daily for four weeks. The animals were sacrificed on the 29th day, using chloroform inhalation method and their liver harvested, processed and stained using Haematoxylin and Eosin method. Stained slides were viewed using light microscope. Result showed normal architecture of the liver, hepatocytes (H), central vein (CV), sinusoid (S) and nucleus in the control group. The cellular architecture of the Alloxan groups D, E, F. G, H and I were all distorted with cytoplasmic vacuolation, sinusoidal spaces dilation and nuclear degeneration. These distortions were severe in the Alloxan only group D. The groups that were administered with VA were able to ameliorate this changes; the groups that were administered with VA two weeks before the administration of the Alloxan (G, H.) had lesser cellular changes than the groups that were administered with Alloxan two weeks before VA administration, (E, F.). The groups that were administered with VA only (B, C.) had a near normal hepatic architecture. VA has cytoprotective potentials that can be used to prevent hepatic damage in diabetic patients.
Peter A. I.,
Azu O. O.,
Edagha I. A.,
Cytoprotective Effects of Ethanolic Extract of Vernonium amygdalina Leaves on Alloxan Induced Hepato-Toxicity in Albino Wistar Rats, American Journal of Life Sciences.
Vol. 3, No. 6,
2015, pp. 395-401.
Ajibade A. J., Fakunle P. B., Ashamu E. A. and Owolabi S. O. Some morphological changes on the kidney of adult Wistar rats following administration of crude extract of Ocimum gratissimum Journal of Medicinal Plants Research 2011; Vol. 5(28), pp. 6435-6438.
Stafford GI, Pedersen ME, van Staden J, Jäger AK. Review on plants with CNS-effects used in traditional South African medicine against mental diseases. J Ethnopharmacol. 2008; 119: 513–37.
Argheore, E. M., Makka H. P. S. Beckerk, (1998) Feed value of some browse plants from central zone of Delta State of Nigeria. Trop. Sci. 38: 97 – 104.
Rao, B. K. and C. H. Rao (2001) Hypoglycemic and antihyperglycemic activity of syzygium alternifolium (wt.) walp seed extracts in normal and diabetic rats. Phytomedicine, 8: 88-93.
Joy, P. P., Thomas, J., Matthew, S. (1998) Medicinal Plants Kerala Agricultural Univ., Kerala, India. Pp 3 – 8.
Rates, S. M (2001) Plant as source of drug. Toxicon – 39(5), 603 – 613.
Tiwari, A. K., Rao, J. M. (2002) Diabetes Mellitus and multiple therapeutic approaches of phytochemicals: present status and future prospects. Current Science, 83(1), 30 – 37.
Biodun Falodun, available at http://imoonline.com.ng/dir/index.php?option=com_content&view=article&id=3386:the-medicinal-benefits-of-vernonia-amygdalina&catid=63:facts&Itemid=77 Retrieved September 20th 2015.
Ifeoma I. Ijeh and Chukwunonso E. C. C. Ejike Current perspectives on the medicinal potentials of Vernonia amygdalina Del. Journal of Medicinal Plants Research, Vol. 5(7), pp. 1051-1061, 2011.
http://www.eduresourceworld.com/2013/10/medicinal-importance-of-bitter-leaf.html Retrieved September 20th 2015.
Izevbigie, E. A., Bryant J. L., Walker A. A novel naturally inhibitor of extracellular signal – regulated kinases and human breast cancer cell growth. Exptal. Biol. Med 229(2): 163 – 169, 2009.
Dallas John. Royal College of Physicians of Edinburgh. Diabetes, Doctor and Dogs: An exhibition on diabetes and endocrinology. 10: 220-23, 2011.
Dwivedi, Girish & Dwivedi Shridhar History of medicine: Sushruta - the Clinician -Teacher par Excellence. National Informatic Centre, pp. 200-204, 2007.
Ekam V. S., 1Ebong P. E., 2Johnson J. T., 2Dasofunjo, K. 1Effect of Activity Directed Fractions of Vernonia amygdalina on Total Body Weight and Blood Glucose Levels of Diabetic Wistar Albino Rats International Journal of Science and Technology ,Volume 2 No. 1, 153-157, 2013.
B. W. Smith and L. A. Adams, “Nonalcoholic fatty liver disease and diabetes mellitus: pathogenesis and treatment,” Nature Reviews Endocrinology, vol. 7, no. 8, pp. 456–465, 2011.
H. Ahmadieh and S. T. Azar, “Liver disease and diabetes: association, pathophysiology, and management,” Diabetes Research and Clinical Practice, vol. 104, no. 1, pp. 53–62, 2014.
Amanda Natália Lucchesi, Lucas Langoni Cassettari, and César Tadeu Spadella Alloxan-Induced Diabetes Causes Morphological and Ultrastructural Changes in Rat Liver that Resemble the Natural History of Chronic Fatty Liver Disease in Humans Journal of Diabetes Research Volume 2015 (2015), Article ID 494578, 11 pages http://dx.doi.org/10.1155/2015/494578.
V. G. Kozyritskij and A. G. Minchenko, “Ultrastructural alterations in rat hepatocytes under diabetes and insulin administration,” Tsitologiya i Genetika, vol. 12, no. 5, pp. 397–401, 1978.
K. Welt, J. Weiss, R. Martin et al., “Ultrastructural, immunohistochemical and biochemical investigations of the rat liver exposed to experimental diabetes and acute hypoxia with and without application of Ginkgo extract,” Experimental and Toxicologic Pathology, vol. 55, no. 5, pp. 331–345, 2004.
R. N. Remedio, A. Castellar, R. A. Barbosa, R. J. Gomes, and F. H. Caetano, “Morphology and protein content of hepatocytes in type I diabetic rats submitted to physical exercises,” Micron, vol. 42, no. 5, pp. 484–491, 2011.
MS Mir, MM Darzi, HM Khan, SA Kamil, AH Sofi, SA Wani Pathomorphological effects of Alloxan induced acute hypoglycaemia in rabbits Alexandria Journal of Medicine, Vol 49, No 4; 343-35, 2013.
P. Evelson, C. Susemihl, I. Villarreal et al., “Hepatic morphological changes and oxidative stress in chronic streptozotocin-diabetic rats,” Annals of Hepatology, vol. 4, no. 2, pp. 115–120, 2005.
N. C. Leite, C. A. Villela-Nogueira, V. L. N. Pannain et al., “Histopathological stages of nonalcoholic fatty liver disease in type 2 diabetes: prevalences and correlated factors,” Liver International, vol. 31, no. 5, pp. 700–706, 2011.
S. E. Regnell and A. Lernmark, “Hepatic steatosis in type 1 diabetes,” Review of Diabetic Studies, vol. 84, no. 4, pp. 454–467, 2011.
J. P. Verderese and Z. Younossi, “Interaction of Type 2 diabetes and nonalcoholic fatty liver disease,” Expert Review of Gastroenterology and Hepatology, vol. 7, no. 5, pp. 405–407, 2013.
O. O. Azu, I. A. Edagha, A. I. Peter, I. Umoh and U. Abia. Extracts of Gongronema latifolium and Vernonia amygdalina improve glycaemia and histomorphology of testes of diabetic Wistar rats African Journal of Pharmacy and Pharmacology, vol 8 (43) 1093 – 1102, 2014.
http://aloksinghpatel.blogspot.com/2012/12/hepatoprotective-activity.html Retrieved September 20th 2015.
Dehmlow, Carola; Erhard, Jochen; De Groot, Herbert. Hepatology Inhibition of Kupffer cell functions as an explanation for the hepatoprotective properties of silibinin. 23 (4), 749-54. 1996.
Jia X.-Y., Zhang Q.-A., Zhang Z.-Q., Wang Y., Yuan J.-F., Wang H.-Y., Zhao D."Hepatoprotective effects of almond oil against carbon tetrachloride induced liver injury in rats" Food Chemistry 2011 125: 2 (673-678).
P.A. Akah, J.A Alemji, O.A Salawu, T.C. Okoye and N.V. OffiahEffects of Vernonia amygdalina on Biochemical and Hematological Parameters in Diabetic Rats Asian Journal of Medical Sciences, 1(3): 108-113. 2009.
P. C. Ekeocha1, T.R. Fasola1, A. H. Ekeocha The effect of vernonia amygdalina on alloxan induced diabetic albino rats African Journal of Food Science and Technology Vol. 3(3) pp. 73-77. 2012.
http://www.mayoclinic.org/diseases-conditions/diabetes/in-depth/diabetes-treatment/art-20044084. Retrieved September 20th 2015.