Inhibitory Effect of Extracts from <i>Datura metel</i> Leaf on Mushroom Tyrosinase

David Morakinyo Sanni; Oluwasegun Victor Omotoyinbo

doi:doi:10.11648/j.ajls.20160402.15

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Inhibitory Effect of Extracts from Datura metel Leaf on Mushroom Tyrosinase

David Morakinyo Sanni, Oluwasegun Victor Omotoyinbo

Published in American Journal of Life Sciences (Volume 4, Issue 2)

Received: 5 February 2016 Accepted: 27 February 2016 Published: 6 May 2016

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Abstract

This study evaluated the tyrosinase-inhibition activities of three extracts; methanol, acetone and dichloromethane of Datura metel leaves. The bioactive components were extracted and then evaluated for their inhibitory effect in vitro on mushroom tyrosinase, using a colorimetric procedure. The Methanolic extract of the plant leaf had the highest inhibition of tyrosinase conversion of substrate L-DOPA with 72.14% inhibition at peak concentration of 400 µg/ml considered unlike their respective acetone (51.08%) and dichloromethane (65.57%) extracts. All the Datura metel extracts had below 50% inhibition between concentrations of 3.1 µg/ml to 200 µg/ml except the methanolic extract which had 53.46% inhibition at 200 µg/ml. Although, there was a steady increase in tyrosinase inhibition for all plant extracts, however none of the plants extracts inhibition exceeded kojic acids inhibition at all concentrations considered.

Published in	American Journal of Life Sciences (Volume 4, Issue 2)
DOI	10.11648/j.ajls.20160402.15
Page(s)	47-50
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Datura metel, Tyrosinase, Inhibition

References

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David Morakinyo Sanni, Oluwasegun Victor Omotoyinbo. (2016). Inhibitory Effect of Extracts from Datura metel Leaf on Mushroom Tyrosinase. American Journal of Life Sciences, 4(2), 47-50. https://doi.org/10.11648/j.ajls.20160402.15

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ACS Style

David Morakinyo Sanni; Oluwasegun Victor Omotoyinbo. Inhibitory Effect of Extracts from Datura metel Leaf on Mushroom Tyrosinase. Am. J. Life Sci. 2016, 4(2), 47-50. doi: 10.11648/j.ajls.20160402.15

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AMA Style

David Morakinyo Sanni, Oluwasegun Victor Omotoyinbo. Inhibitory Effect of Extracts from Datura metel Leaf on Mushroom Tyrosinase. Am J Life Sci. 2016;4(2):47-50. doi: 10.11648/j.ajls.20160402.15

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@article{10.11648/j.ajls.20160402.15,
  author = {David Morakinyo Sanni and Oluwasegun Victor Omotoyinbo},
  title = {Inhibitory Effect of Extracts from Datura metel Leaf on Mushroom Tyrosinase},
  journal = {American Journal of Life Sciences},
  volume = {4},
  number = {2},
  pages = {47-50},
  doi = {10.11648/j.ajls.20160402.15},
  url = {https://doi.org/10.11648/j.ajls.20160402.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20160402.15},
  abstract = {This study evaluated the tyrosinase-inhibition activities of three extracts; methanol, acetone and dichloromethane of Datura metel leaves. The bioactive components were extracted and then evaluated for their inhibitory effect in vitro on mushroom tyrosinase, using a colorimetric procedure. The Methanolic extract of the plant leaf had the highest inhibition of tyrosinase conversion of substrate L-DOPA with 72.14% inhibition at peak concentration of 400 µg/ml considered unlike their respective acetone (51.08%) and dichloromethane (65.57%) extracts. All the Datura metel extracts had below 50% inhibition between concentrations of 3.1 µg/ml to 200 µg/ml except the methanolic extract which had 53.46% inhibition at 200 µg/ml. Although, there was a steady increase in tyrosinase inhibition for all plant extracts, however none of the plants extracts inhibition exceeded kojic acids inhibition at all concentrations considered.},
 year = {2016}
}

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TY  - JOUR
T1  - Inhibitory Effect of Extracts from Datura metel Leaf on Mushroom Tyrosinase
AU  - David Morakinyo Sanni
AU  - Oluwasegun Victor Omotoyinbo
Y1  - 2016/05/06
PY  - 2016
N1  - https://doi.org/10.11648/j.ajls.20160402.15
DO  - 10.11648/j.ajls.20160402.15
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 47
EP  - 50
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.20160402.15
AB  - This study evaluated the tyrosinase-inhibition activities of three extracts; methanol, acetone and dichloromethane of Datura metel leaves. The bioactive components were extracted and then evaluated for their inhibitory effect in vitro on mushroom tyrosinase, using a colorimetric procedure. The Methanolic extract of the plant leaf had the highest inhibition of tyrosinase conversion of substrate L-DOPA with 72.14% inhibition at peak concentration of 400 µg/ml considered unlike their respective acetone (51.08%) and dichloromethane (65.57%) extracts. All the Datura metel extracts had below 50% inhibition between concentrations of 3.1 µg/ml to 200 µg/ml except the methanolic extract which had 53.46% inhibition at 200 µg/ml. Although, there was a steady increase in tyrosinase inhibition for all plant extracts, however none of the plants extracts inhibition exceeded kojic acids inhibition at all concentrations considered.
VL  - 4
IS  - 2
ER  -

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Author Information

David Morakinyo Sanni

Department of Biochemistry, Federal University of Technology, Akure, Ondo State, Nigeria
Oluwasegun Victor Omotoyinbo

Department of Biochemistry, Federal University of Technology, Akure, Ondo State, Nigeria

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Plain Text BibTeX RIS

APA Style

David Morakinyo Sanni, Oluwasegun Victor Omotoyinbo. (2016). Inhibitory Effect of Extracts from Datura metel Leaf on Mushroom Tyrosinase. American Journal of Life Sciences, 4(2), 47-50. https://doi.org/10.11648/j.ajls.20160402.15

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ACS Style

David Morakinyo Sanni; Oluwasegun Victor Omotoyinbo. Inhibitory Effect of Extracts from Datura metel Leaf on Mushroom Tyrosinase. Am. J. Life Sci. 2016, 4(2), 47-50. doi: 10.11648/j.ajls.20160402.15

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AMA Style

David Morakinyo Sanni, Oluwasegun Victor Omotoyinbo. Inhibitory Effect of Extracts from Datura metel Leaf on Mushroom Tyrosinase. Am J Life Sci. 2016;4(2):47-50. doi: 10.11648/j.ajls.20160402.15

Copy | Download

@article{10.11648/j.ajls.20160402.15,
  author = {David Morakinyo Sanni and Oluwasegun Victor Omotoyinbo},
  title = {Inhibitory Effect of Extracts from Datura metel Leaf on Mushroom Tyrosinase},
  journal = {American Journal of Life Sciences},
  volume = {4},
  number = {2},
  pages = {47-50},
  doi = {10.11648/j.ajls.20160402.15},
  url = {https://doi.org/10.11648/j.ajls.20160402.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20160402.15},
  abstract = {This study evaluated the tyrosinase-inhibition activities of three extracts; methanol, acetone and dichloromethane of Datura metel leaves. The bioactive components were extracted and then evaluated for their inhibitory effect in vitro on mushroom tyrosinase, using a colorimetric procedure. The Methanolic extract of the plant leaf had the highest inhibition of tyrosinase conversion of substrate L-DOPA with 72.14% inhibition at peak concentration of 400 µg/ml considered unlike their respective acetone (51.08%) and dichloromethane (65.57%) extracts. All the Datura metel extracts had below 50% inhibition between concentrations of 3.1 µg/ml to 200 µg/ml except the methanolic extract which had 53.46% inhibition at 200 µg/ml. Although, there was a steady increase in tyrosinase inhibition for all plant extracts, however none of the plants extracts inhibition exceeded kojic acids inhibition at all concentrations considered.},
 year = {2016}
}

Copy | Download

TY  - JOUR
T1  - Inhibitory Effect of Extracts from Datura metel Leaf on Mushroom Tyrosinase
AU  - David Morakinyo Sanni
AU  - Oluwasegun Victor Omotoyinbo
Y1  - 2016/05/06
PY  - 2016
N1  - https://doi.org/10.11648/j.ajls.20160402.15
DO  - 10.11648/j.ajls.20160402.15
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 47
EP  - 50
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.20160402.15
AB  - This study evaluated the tyrosinase-inhibition activities of three extracts; methanol, acetone and dichloromethane of Datura metel leaves. The bioactive components were extracted and then evaluated for their inhibitory effect in vitro on mushroom tyrosinase, using a colorimetric procedure. The Methanolic extract of the plant leaf had the highest inhibition of tyrosinase conversion of substrate L-DOPA with 72.14% inhibition at peak concentration of 400 µg/ml considered unlike their respective acetone (51.08%) and dichloromethane (65.57%) extracts. All the Datura metel extracts had below 50% inhibition between concentrations of 3.1 µg/ml to 200 µg/ml except the methanolic extract which had 53.46% inhibition at 200 µg/ml. Although, there was a steady increase in tyrosinase inhibition for all plant extracts, however none of the plants extracts inhibition exceeded kojic acids inhibition at all concentrations considered.
VL  - 4
IS  - 2
ER  -

Copy | Download