In vitro Propagation of Dendrobium jerdonianum Wight Through Flower Stalk, Leaf, Nodes/ Internodes as Explants
American Journal of Life Sciences
Volume 4, Issue 3, June 2016, Pages: 76-81
Received: Apr. 29, 2016;
Accepted: May 20, 2016;
Published: Jun. 17, 2016
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Sr. Sagaya Mary B., Plant Tissue Culture Division, Department of Botany, St. Joseph’s Post-Graduate Studies and Research Centre, Bangalore, India
Divakar K. M., Plant Tissue Culture Division, Department of Botany, St. Joseph’s Post-Graduate Studies and Research Centre, Bangalore, India
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Dendrobium jerdonianum Wight is an Epiphytic tufted orchid at higher elevations. The maximum percentage establishment of Flower stalk, leaf, node and internode explants was observed on MS, VW and KC Medium. However, MS media fortified with activated charcoal exhibited maximum multiplication rate for in vitro rooting. MS, VW and KC media supplemented with various concentrations of auxins and cytokinins were used in flower stalk, leaf, nodes and internodes for plantlet formation. In the evaluation of the media MS basal medium fortified with 0.5mg 2, 4, D-dichloro phenoxy acetic acid +5mg BAP + 50mlsuitable for flower stalk culture. KC medium equipped with 0.5mg 2, 4, D-dichloro phenoxy acetic acid was found to be suitable for Leaf and internodes Culture. VW medium equipped with 0.5mg 2, 4, D-dichloro phenoxy acetic acid + 3mg BAP + 50ml CM, was found to be suitable for nodal segments. The entire above medium used with different composition gives highest percentage of 80-95 percent results for plantlet formation. In vitro rooting was successful with MS medium supplemented with 0.5mg 2, 4, D-dichloro phenoxy acetic acid + 5mg IAA + 50ml CM and 500mg of activated charcoal. 90 days old in vitro plantlets were hardened and transferred to green house after ex vitro rooting technique. Significance of the present work is discussed here.
Plantlet Formation, Explants, Dendrobium jerdonianum, Propagation, Establishment
To cite this article
Sr. Sagaya Mary B.,
Divakar K. M.,
In vitro Propagation of Dendrobium jerdonianum Wight Through Flower Stalk, Leaf, Nodes/ Internodes as Explants, American Journal of Life Sciences.
Vol. 4, No. 3,
2016, pp. 76-81.
Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/
) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Ahmedullah, M. & Nayar M. P. (1987). Endemic Plants of the Indian region- Vol 1. Peninsular India, Botanical Survey of India, Calcutta, 262pp.
Aktat, S. Nasiruddin, K. M. & Huq, H. (2007). In vitro root formation in Dendrobium orchid plantlets with IBA. J Agr. Rural Dev. 5: 48–51.
Arditti, J. (1977). Clonal propagation of orchids by means of tissue culture-a manual. In J. Arditti (ed.). Orchid biology. vol. I, 203-293. Cornell University Press. New York. USA.
Arditti, J. and Ernst, R. (1993). Micropropagation of Orchids. Wiley and Sons. New York. USA.
Blatter, E. (1928). A list of orchids with some new species from High Wavy Mountain (Madurai District). Journal of the Bombay Natural History Society 32: 518–523
CSS451 Plant Tissue Culture, (2010).
Fay, M. E. (1994). In what situations is in vitro appropriate to plant conservation. Biodiv. Conserv. 3: 176–183.
Govaerts et al., (2012). Endemic orchids of Western Ghats.
Hooker, J. D. (1888–1890). Flora of British India. Vols. 5 & 6. L. Reeve & Co. London. 667–858 & 1–198pp.
Jeewan Singh Jalal, & Jayanthi, J. (2012). Endemic orchids of peninsular India: a review, Botanical Survey of India, Western Regional Centre, Maharashtra 4 (15): 3415-3425.
Mittermeier, (2000). Hotspots: Earth’s Biologically Richest and Most Endangered Terrestrial Eco regions. Cemex/Conservation International 431 pp.
Mohanan, M. and Balakrishna, N. P. (1991). Endangered orchids of Nilgiris Biosphere Reserve India. Kerala Forest Department, Thiruvananthapuram. Pp. 187-199.
Nayar, M. P. (1996). Hot Spots of Endemic Plants of India, Nepal and Bhutan. Tropical Botanic Garden and Research Institute. Thiruvananthapuram. 252pp.
Orange Kerala Dendrobium, http://www.flowersofindia.net/)
Park, SY., Murthy, H. N & Paek, K. Y (2002), Rapid propagation of Phalaenopsis from floral stalk-derived leaves. In vitro Cell Dev Biol Plant. 38 (2002) 168.
Subramanian, K. & Nayar, M. P. (1974). Vegetation and phytogeography of the Western Ghats, pp. 178–196.
W3 Tropicos. Kew Monocot list, International Plant Names Index (IPNI).