American Journal of Life Sciences

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Biological Potentials of Ginger Associated Streptomyces Compared with Ginger Essential Oil

Received: 11 October 2016    Accepted: 25 October 2016    Published: 02 December 2016
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Abstract

Medicinal plants and associated microorganisms are recognized to have beneficial relationship. These two organisms are well known for their ability to produce bioactive secondary metabolites which the similarity has been demonstrated in a few works. This study had for objective to assess biological potentials of actinomycetes isolated from ginger rhizomes and its rhizospheric soil and to determine their similarity and efficiency with ginger essential oil. Among the 63 actinomycetes strains isolated from the rhizomes and rhizospheric soils of two ginger countries plantations of Soavinandriana Itasy-Madagascar, biological activity tests showed that 16 strains (2 endophytes and 14 from rhizospheric soils of ginger) exhibited antimicrobial activity against at least one germ. The strains are more active against Gram+ bacteria and fungi than Gram- bacteria. Only, one strain isolated from ginger rhizospheric soil of the site n°2 (AHO 18) inhibited the development of all tests germs. The tests conducted on six representative strains selected on the basis of antimicrobial assay showed that extracts from the isolates AHO 3 and AHO 43 have strong antiproliferative activity on cells HT-20 (colon cancer) with IC50 values of 5µg/ml and 2,2μg/ml, respectively; strong antimalaria activity against the chloroquino-resistant Plasmodium falciparum strain (IC50=1,25μg/ml for AHO 3 extract and 2,5<IC50<5µg/ml for AHO 43 extract) and antioxidant activity (IC50=15mg/ml for AHO 3 extract and 10,6mg/ml for AHO 43 extract). The 2 isolates based on phenotypic and molecular characterization using their 16S rRNA gene were identified as Streptomyces chrysomallus (isolate AHO 3) and Streptomyces sp (isolate AHO 43). Moreover, the two essential oils of ginger tested showed antimicrobial activity against all tests germs used and antioxidant activity. Only, ginger essential oil from the site n°2 exhibited moderate antiproliferative potential (IC50=14μg/ml) on colon cancer cells and high antiplasmodial activity (2,5<IC50<5µg/ml). Streptomyces sp showed similar and strong biological activities than those of ginger essential oil from the site n°2. Chemical screening of the Streptomyces sp extract and the essential oil H2 revealed the common presence of terpens and phenolic compounds.

DOI 10.11648/j.ajls.20160406.13
Published in American Journal of Life Sciences (Volume 4, Issue 6, December 2016)
Page(s) 152-163
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This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Actinomycetes, Endophytes, Rhizospheric Soil, Rhizome, Ginger, Essential Oil, Antimicrobial, Antioxidant, Antimalaria, Antiproliferative

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Author Information
  • National Centre of Environmental Research, Antananarivo, Madagascar

  • National Centre of Environmental Research, Antananarivo, Madagascar

  • National Centre of Environmental Research, Antananarivo, Madagascar

  • National Centre of Environmental Research, Antananarivo, Madagascar

  • Department of Fundamental and Applied Biochemistry, Faculty of Sciences, University of Antananarivo, Antananarivo, Madagascar

  • Ministry of Health, Antananarivo, Madagascar

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    Herivony Onja Andriambeloson, Rado Rasolomampianina, Rahanira Ralambondrahety, Rigobert Andrianantenaina, Marson Raherimandimby, et al. (2016). Biological Potentials of Ginger Associated Streptomyces Compared with Ginger Essential Oil. American Journal of Life Sciences, 4(6), 152-163. https://doi.org/10.11648/j.ajls.20160406.13

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    Herivony Onja Andriambeloson; Rado Rasolomampianina; Rahanira Ralambondrahety; Rigobert Andrianantenaina; Marson Raherimandimby, et al. Biological Potentials of Ginger Associated Streptomyces Compared with Ginger Essential Oil. Am. J. Life Sci. 2016, 4(6), 152-163. doi: 10.11648/j.ajls.20160406.13

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    Herivony Onja Andriambeloson, Rado Rasolomampianina, Rahanira Ralambondrahety, Rigobert Andrianantenaina, Marson Raherimandimby, et al. Biological Potentials of Ginger Associated Streptomyces Compared with Ginger Essential Oil. Am J Life Sci. 2016;4(6):152-163. doi: 10.11648/j.ajls.20160406.13

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  • @article{10.11648/j.ajls.20160406.13,
      author = {Herivony Onja Andriambeloson and Rado Rasolomampianina and Rahanira Ralambondrahety and Rigobert Andrianantenaina and Marson Raherimandimby and Fidèle Randriamiharisoa},
      title = {Biological Potentials of Ginger Associated Streptomyces Compared with Ginger Essential Oil},
      journal = {American Journal of Life Sciences},
      volume = {4},
      number = {6},
      pages = {152-163},
      doi = {10.11648/j.ajls.20160406.13},
      url = {https://doi.org/10.11648/j.ajls.20160406.13},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ajls.20160406.13},
      abstract = {Medicinal plants and associated microorganisms are recognized to have beneficial relationship. These two organisms are well known for their ability to produce bioactive secondary metabolites which the similarity has been demonstrated in a few works. This study had for objective to assess biological potentials of actinomycetes isolated from ginger rhizomes and its rhizospheric soil and to determine their similarity and efficiency with ginger essential oil. Among the 63 actinomycetes strains isolated from the rhizomes and rhizospheric soils of two ginger countries plantations of Soavinandriana Itasy-Madagascar, biological activity tests showed that 16 strains (2 endophytes and 14 from rhizospheric soils of ginger) exhibited antimicrobial activity against at least one germ. The strains are more active against Gram+ bacteria and fungi than Gram- bacteria. Only, one strain isolated from ginger rhizospheric soil of the site n°2 (AHO 18) inhibited the development of all tests germs. The tests conducted on six representative strains selected on the basis of antimicrobial assay showed that extracts from the isolates AHO 3 and AHO 43 have strong antiproliferative activity on cells HT-20 (colon cancer) with IC50 values of 5µg/ml and 2,2μg/ml, respectively; strong antimalaria activity against the chloroquino-resistant Plasmodium falciparum strain (IC50=1,25μg/ml for AHO 3 extract and 2,5<IC50<5µg/ml for AHO 43 extract) and antioxidant activity (IC50=15mg/ml for AHO 3 extract and 10,6mg/ml for AHO 43 extract). The 2 isolates based on phenotypic and molecular characterization using their 16S rRNA gene were identified as Streptomyces chrysomallus (isolate AHO 3) and Streptomyces sp (isolate AHO 43). Moreover, the two essential oils of ginger tested showed antimicrobial activity against all tests germs used and antioxidant activity. Only, ginger essential oil from the site n°2 exhibited moderate antiproliferative potential (IC50=14μg/ml) on colon cancer cells and high antiplasmodial activity (2,5<IC50<5µg/ml). Streptomyces sp showed similar and strong biological activities than those of ginger essential oil from the site n°2. Chemical screening of the Streptomyces sp extract and the essential oil H2 revealed the common presence of terpens and phenolic compounds.},
     year = {2016}
    }
    

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  • TY  - JOUR
    T1  - Biological Potentials of Ginger Associated Streptomyces Compared with Ginger Essential Oil
    AU  - Herivony Onja Andriambeloson
    AU  - Rado Rasolomampianina
    AU  - Rahanira Ralambondrahety
    AU  - Rigobert Andrianantenaina
    AU  - Marson Raherimandimby
    AU  - Fidèle Randriamiharisoa
    Y1  - 2016/12/02
    PY  - 2016
    N1  - https://doi.org/10.11648/j.ajls.20160406.13
    DO  - 10.11648/j.ajls.20160406.13
    T2  - American Journal of Life Sciences
    JF  - American Journal of Life Sciences
    JO  - American Journal of Life Sciences
    SP  - 152
    EP  - 163
    PB  - Science Publishing Group
    SN  - 2328-5737
    UR  - https://doi.org/10.11648/j.ajls.20160406.13
    AB  - Medicinal plants and associated microorganisms are recognized to have beneficial relationship. These two organisms are well known for their ability to produce bioactive secondary metabolites which the similarity has been demonstrated in a few works. This study had for objective to assess biological potentials of actinomycetes isolated from ginger rhizomes and its rhizospheric soil and to determine their similarity and efficiency with ginger essential oil. Among the 63 actinomycetes strains isolated from the rhizomes and rhizospheric soils of two ginger countries plantations of Soavinandriana Itasy-Madagascar, biological activity tests showed that 16 strains (2 endophytes and 14 from rhizospheric soils of ginger) exhibited antimicrobial activity against at least one germ. The strains are more active against Gram+ bacteria and fungi than Gram- bacteria. Only, one strain isolated from ginger rhizospheric soil of the site n°2 (AHO 18) inhibited the development of all tests germs. The tests conducted on six representative strains selected on the basis of antimicrobial assay showed that extracts from the isolates AHO 3 and AHO 43 have strong antiproliferative activity on cells HT-20 (colon cancer) with IC50 values of 5µg/ml and 2,2μg/ml, respectively; strong antimalaria activity against the chloroquino-resistant Plasmodium falciparum strain (IC50=1,25μg/ml for AHO 3 extract and 2,5<IC50<5µg/ml for AHO 43 extract) and antioxidant activity (IC50=15mg/ml for AHO 3 extract and 10,6mg/ml for AHO 43 extract). The 2 isolates based on phenotypic and molecular characterization using their 16S rRNA gene were identified as Streptomyces chrysomallus (isolate AHO 3) and Streptomyces sp (isolate AHO 43). Moreover, the two essential oils of ginger tested showed antimicrobial activity against all tests germs used and antioxidant activity. Only, ginger essential oil from the site n°2 exhibited moderate antiproliferative potential (IC50=14μg/ml) on colon cancer cells and high antiplasmodial activity (2,5<IC50<5µg/ml). Streptomyces sp showed similar and strong biological activities than those of ginger essential oil from the site n°2. Chemical screening of the Streptomyces sp extract and the essential oil H2 revealed the common presence of terpens and phenolic compounds.
    VL  - 4
    IS  - 6
    ER  - 

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