Restraint-Induced Glucocorticoid Receptor Downregulation is Dysregulated in High Fat Diet-Fed Rats Likely from Impairment of miR-142-3p Expression in the Hypothalamus and Hippocampus

Takahiro Nemoto; Yoshihiko Kakinuma; Tamotsu Sibasaki

doi:doi:10.11648/j.ajls.s.2015030302.15

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Restraint-Induced Glucocorticoid Receptor Downregulation is Dysregulated in High Fat Diet-Fed Rats Likely from Impairment of miR-142-3p Expression in the Hypothalamus and Hippocampus

Takahiro Nemoto, Yoshihiko Kakinuma, Tamotsu Sibasaki

Published in American Journal of Life Sciences (Volume 3, Issue 3-2)

Received: 25 February 2015 Accepted: 25 March 2015 Published: 6 May 2015

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Abstract

High fat diet (HFD) induces dysregulation of the hypothalamic-pituitary-adrenal (HPA) axis function. The HPA axis is controlled by the feedback of glucocortioids on the hypothalamus, hippocampus and pituitary. At least three miRNAs (miR-101a, miR-124, miR-142-3p) have been reported to suppress glucocorticoid receptor (GR) translation. Because their relation to stress-induced downregulation of GR expression and dysregulation of its expression in HFD feeding are unclear, we studied to identify which miRNAs are involved in restraint-induced downregulation of GR expression in the hypothalamus and hippocampus, and to compare the basal and restraint-modified miRNA expressions in these tissues in HFD-fed rats. Rats exposed to HFD were divided into two groups, HFD-induced obese (HFD-ob) and obesity resistant (HFD-obR) rats. Basal plasma corticosterone concentrations were higher in HFD-ob than in standard chow-fed (SC) rats and in HFD-obR. Restraint-induced elevation of plasma corticosterone was higher in HFD-obR than in the other groups. Restraint decreased GR expressions and increased miR-142-3p expression in the hypothalamus and hippocampus without affecting others expressions. miR-142-3p expressions in both areas were increased by dexamethasone and restraint-induced miR-142-3p expression was blocked in adrenalectomy. The basal expression of GR or miR-142-3p expression in both areas of HFD-fed rats did not differ from those of SC, and restraint induced no changes in GR or miR-142-3p expression in both areas in HFD-ob and HFD-obR. These results suggest that impairment of glucocorticoid-induced increase in miR-142-3p may be involved in dysregulation of stress-induced downregulation of GR expression in the hypothalamus and hippocampus of HFD-fed rats.

Published in	American Journal of Life Sciences (Volume 3, Issue 3-2) This article belongs to the Special Issue Biology and Medicine of Peptide and Steroid Hormones
DOI	10.11648/j.ajls.s.2015030302.15
Page(s)	24-30
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Stress, High Fat Diet, Hypothalamus, Hippocampus, microRNA

References

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Takahiro Nemoto, Yoshihiko Kakinuma, Tamotsu Sibasaki. (2015). Restraint-Induced Glucocorticoid Receptor Downregulation is Dysregulated in High Fat Diet-Fed Rats Likely from Impairment of miR-142-3p Expression in the Hypothalamus and Hippocampus. American Journal of Life Sciences, 3(3-2), 24-30. https://doi.org/10.11648/j.ajls.s.2015030302.15

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Takahiro Nemoto; Yoshihiko Kakinuma; Tamotsu Sibasaki. Restraint-Induced Glucocorticoid Receptor Downregulation is Dysregulated in High Fat Diet-Fed Rats Likely from Impairment of miR-142-3p Expression in the Hypothalamus and Hippocampus. Am. J. Life Sci. 2015, 3(3-2), 24-30. doi: 10.11648/j.ajls.s.2015030302.15

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AMA Style

Takahiro Nemoto, Yoshihiko Kakinuma, Tamotsu Sibasaki. Restraint-Induced Glucocorticoid Receptor Downregulation is Dysregulated in High Fat Diet-Fed Rats Likely from Impairment of miR-142-3p Expression in the Hypothalamus and Hippocampus. Am J Life Sci. 2015;3(3-2):24-30. doi: 10.11648/j.ajls.s.2015030302.15

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@article{10.11648/j.ajls.s.2015030302.15,
  author = {Takahiro Nemoto and Yoshihiko Kakinuma and Tamotsu Sibasaki},
  title = {Restraint-Induced Glucocorticoid Receptor Downregulation is Dysregulated in High Fat Diet-Fed Rats Likely from Impairment of miR-142-3p Expression in the Hypothalamus and Hippocampus},
  journal = {American Journal of Life Sciences},
  volume = {3},
  number = {3-2},
  pages = {24-30},
  doi = {10.11648/j.ajls.s.2015030302.15},
  url = {https://doi.org/10.11648/j.ajls.s.2015030302.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.s.2015030302.15},
  abstract = {High fat diet (HFD) induces dysregulation of the hypothalamic-pituitary-adrenal (HPA) axis function. The HPA axis is controlled by the feedback of glucocortioids on the hypothalamus, hippocampus and pituitary. At least three miRNAs (miR-101a, miR-124, miR-142-3p) have been reported to suppress glucocorticoid receptor (GR) translation. Because their relation to stress-induced downregulation of GR expression and dysregulation of its expression in HFD feeding are unclear, we studied to identify which miRNAs are involved in restraint-induced downregulation of GR expression in the hypothalamus and hippocampus, and to compare the basal and restraint-modified miRNA expressions in these tissues in HFD-fed rats. Rats exposed to HFD were divided into two groups, HFD-induced obese (HFD-ob) and obesity resistant (HFD-obR) rats. Basal plasma corticosterone concentrations were higher in HFD-ob than in standard chow-fed (SC) rats and in HFD-obR. Restraint-induced elevation of plasma corticosterone was higher in HFD-obR than in the other groups. Restraint decreased GR expressions and increased miR-142-3p expression in the hypothalamus and hippocampus without affecting others expressions. miR-142-3p expressions in both areas were increased by dexamethasone and restraint-induced miR-142-3p expression was blocked in adrenalectomy. The basal expression of GR or miR-142-3p expression in both areas of HFD-fed rats did not differ from those of SC, and restraint induced no changes in GR or miR-142-3p expression in both areas in HFD-ob and HFD-obR. These results suggest that impairment of glucocorticoid-induced increase in miR-142-3p may be involved in dysregulation of stress-induced downregulation of GR expression in the hypothalamus and hippocampus of HFD-fed rats.},
 year = {2015}
}

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TY  - JOUR
T1  - Restraint-Induced Glucocorticoid Receptor Downregulation is Dysregulated in High Fat Diet-Fed Rats Likely from Impairment of miR-142-3p Expression in the Hypothalamus and Hippocampus
AU  - Takahiro Nemoto
AU  - Yoshihiko Kakinuma
AU  - Tamotsu Sibasaki
Y1  - 2015/05/06
PY  - 2015
N1  - https://doi.org/10.11648/j.ajls.s.2015030302.15
DO  - 10.11648/j.ajls.s.2015030302.15
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 24
EP  - 30
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.s.2015030302.15
AB  - High fat diet (HFD) induces dysregulation of the hypothalamic-pituitary-adrenal (HPA) axis function. The HPA axis is controlled by the feedback of glucocortioids on the hypothalamus, hippocampus and pituitary. At least three miRNAs (miR-101a, miR-124, miR-142-3p) have been reported to suppress glucocorticoid receptor (GR) translation. Because their relation to stress-induced downregulation of GR expression and dysregulation of its expression in HFD feeding are unclear, we studied to identify which miRNAs are involved in restraint-induced downregulation of GR expression in the hypothalamus and hippocampus, and to compare the basal and restraint-modified miRNA expressions in these tissues in HFD-fed rats. Rats exposed to HFD were divided into two groups, HFD-induced obese (HFD-ob) and obesity resistant (HFD-obR) rats. Basal plasma corticosterone concentrations were higher in HFD-ob than in standard chow-fed (SC) rats and in HFD-obR. Restraint-induced elevation of plasma corticosterone was higher in HFD-obR than in the other groups. Restraint decreased GR expressions and increased miR-142-3p expression in the hypothalamus and hippocampus without affecting others expressions. miR-142-3p expressions in both areas were increased by dexamethasone and restraint-induced miR-142-3p expression was blocked in adrenalectomy. The basal expression of GR or miR-142-3p expression in both areas of HFD-fed rats did not differ from those of SC, and restraint induced no changes in GR or miR-142-3p expression in both areas in HFD-ob and HFD-obR. These results suggest that impairment of glucocorticoid-induced increase in miR-142-3p may be involved in dysregulation of stress-induced downregulation of GR expression in the hypothalamus and hippocampus of HFD-fed rats.
VL  - 3
IS  - 3-2
ER  -

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Author Information

Takahiro Nemoto

Dept. Physiology, Nippon Medical School, Tokyo, Japan
Yoshihiko Kakinuma

Dept. Physiology, Nippon Medical School, Tokyo, Japan
Tamotsu Sibasaki

Dept. Physiology, Nippon Medical School, Tokyo, Japan

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Takahiro Nemoto, Yoshihiko Kakinuma, Tamotsu Sibasaki. (2015). Restraint-Induced Glucocorticoid Receptor Downregulation is Dysregulated in High Fat Diet-Fed Rats Likely from Impairment of miR-142-3p Expression in the Hypothalamus and Hippocampus. American Journal of Life Sciences, 3(3-2), 24-30. https://doi.org/10.11648/j.ajls.s.2015030302.15

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Takahiro Nemoto; Yoshihiko Kakinuma; Tamotsu Sibasaki. Restraint-Induced Glucocorticoid Receptor Downregulation is Dysregulated in High Fat Diet-Fed Rats Likely from Impairment of miR-142-3p Expression in the Hypothalamus and Hippocampus. Am. J. Life Sci. 2015, 3(3-2), 24-30. doi: 10.11648/j.ajls.s.2015030302.15

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AMA Style

Takahiro Nemoto, Yoshihiko Kakinuma, Tamotsu Sibasaki. Restraint-Induced Glucocorticoid Receptor Downregulation is Dysregulated in High Fat Diet-Fed Rats Likely from Impairment of miR-142-3p Expression in the Hypothalamus and Hippocampus. Am J Life Sci. 2015;3(3-2):24-30. doi: 10.11648/j.ajls.s.2015030302.15

Copy | Download

@article{10.11648/j.ajls.s.2015030302.15,
  author = {Takahiro Nemoto and Yoshihiko Kakinuma and Tamotsu Sibasaki},
  title = {Restraint-Induced Glucocorticoid Receptor Downregulation is Dysregulated in High Fat Diet-Fed Rats Likely from Impairment of miR-142-3p Expression in the Hypothalamus and Hippocampus},
  journal = {American Journal of Life Sciences},
  volume = {3},
  number = {3-2},
  pages = {24-30},
  doi = {10.11648/j.ajls.s.2015030302.15},
  url = {https://doi.org/10.11648/j.ajls.s.2015030302.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.s.2015030302.15},
  abstract = {High fat diet (HFD) induces dysregulation of the hypothalamic-pituitary-adrenal (HPA) axis function. The HPA axis is controlled by the feedback of glucocortioids on the hypothalamus, hippocampus and pituitary. At least three miRNAs (miR-101a, miR-124, miR-142-3p) have been reported to suppress glucocorticoid receptor (GR) translation. Because their relation to stress-induced downregulation of GR expression and dysregulation of its expression in HFD feeding are unclear, we studied to identify which miRNAs are involved in restraint-induced downregulation of GR expression in the hypothalamus and hippocampus, and to compare the basal and restraint-modified miRNA expressions in these tissues in HFD-fed rats. Rats exposed to HFD were divided into two groups, HFD-induced obese (HFD-ob) and obesity resistant (HFD-obR) rats. Basal plasma corticosterone concentrations were higher in HFD-ob than in standard chow-fed (SC) rats and in HFD-obR. Restraint-induced elevation of plasma corticosterone was higher in HFD-obR than in the other groups. Restraint decreased GR expressions and increased miR-142-3p expression in the hypothalamus and hippocampus without affecting others expressions. miR-142-3p expressions in both areas were increased by dexamethasone and restraint-induced miR-142-3p expression was blocked in adrenalectomy. The basal expression of GR or miR-142-3p expression in both areas of HFD-fed rats did not differ from those of SC, and restraint induced no changes in GR or miR-142-3p expression in both areas in HFD-ob and HFD-obR. These results suggest that impairment of glucocorticoid-induced increase in miR-142-3p may be involved in dysregulation of stress-induced downregulation of GR expression in the hypothalamus and hippocampus of HFD-fed rats.},
 year = {2015}
}

Copy | Download

TY  - JOUR
T1  - Restraint-Induced Glucocorticoid Receptor Downregulation is Dysregulated in High Fat Diet-Fed Rats Likely from Impairment of miR-142-3p Expression in the Hypothalamus and Hippocampus
AU  - Takahiro Nemoto
AU  - Yoshihiko Kakinuma
AU  - Tamotsu Sibasaki
Y1  - 2015/05/06
PY  - 2015
N1  - https://doi.org/10.11648/j.ajls.s.2015030302.15
DO  - 10.11648/j.ajls.s.2015030302.15
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 24
EP  - 30
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.s.2015030302.15
AB  - High fat diet (HFD) induces dysregulation of the hypothalamic-pituitary-adrenal (HPA) axis function. The HPA axis is controlled by the feedback of glucocortioids on the hypothalamus, hippocampus and pituitary. At least three miRNAs (miR-101a, miR-124, miR-142-3p) have been reported to suppress glucocorticoid receptor (GR) translation. Because their relation to stress-induced downregulation of GR expression and dysregulation of its expression in HFD feeding are unclear, we studied to identify which miRNAs are involved in restraint-induced downregulation of GR expression in the hypothalamus and hippocampus, and to compare the basal and restraint-modified miRNA expressions in these tissues in HFD-fed rats. Rats exposed to HFD were divided into two groups, HFD-induced obese (HFD-ob) and obesity resistant (HFD-obR) rats. Basal plasma corticosterone concentrations were higher in HFD-ob than in standard chow-fed (SC) rats and in HFD-obR. Restraint-induced elevation of plasma corticosterone was higher in HFD-obR than in the other groups. Restraint decreased GR expressions and increased miR-142-3p expression in the hypothalamus and hippocampus without affecting others expressions. miR-142-3p expressions in both areas were increased by dexamethasone and restraint-induced miR-142-3p expression was blocked in adrenalectomy. The basal expression of GR or miR-142-3p expression in both areas of HFD-fed rats did not differ from those of SC, and restraint induced no changes in GR or miR-142-3p expression in both areas in HFD-ob and HFD-obR. These results suggest that impairment of glucocorticoid-induced increase in miR-142-3p may be involved in dysregulation of stress-induced downregulation of GR expression in the hypothalamus and hippocampus of HFD-fed rats.
VL  - 3
IS  - 3-2
ER  -

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