International Journal of Nutrition and Food Sciences
Volume 4, Issue 6, November 2015, Pages: 688-694
Received: Oct. 19, 2015;
Accepted: Nov. 9, 2015;
Published: Dec. 21, 2015
Views 13936 Downloads 111
Mahendra Kumar Trivedi, Trivedi Global Inc., Henderson, USA
Alice Branton, Trivedi Global Inc., Henderson, USA
Dahryn Trivedi, Trivedi Global Inc., Henderson, USA
Gopal Nayak, Trivedi Global Inc., Henderson, USA
Mayank Gangwar, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
Snehasis Jana, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
The objective of the study was to assess the growth contributing characters of biofield treated bottle gourd (Lagenaria siceraria) and okra (Abelmoschus esculentus) seeds. The seeds of both crops were divided into two groups, one was kept aside and denoted as untreated, while the other group was subjected biofield energy treatment. The variabilities in growth contributing parameters were studied and compared with their control. Further the level of glutathione (GSH) in okra leaves, along with DNA fingerprinting in bottle gourd were analyzed using RAPD method. After germination, the plants of bottle gourd were reported to be strong and erect with better canopy as compared with the control. The vegetative growth of okra plants after biofield energy treatment was found to be stout with small canopy, strong steam, and more fruits per nodes, that contributed high yield as compared with the control. However, endogenous level of GSH in the leaves of okra was increased by 47.65% as compared to the untreated group, which may suggest an improved immunity of okra crops. Besides, the DNA fingerprinting data, showed polymorphism (42%) between treated and untreated samples of bottle gourd. The overall results suggest that the biofield energy treatment on bottle gourd and okra seeds, results an improved overall growth of plant and yield, which may enhance flowering and fruiting per plant. Study results conclude that the biofield energy treatment could be an alternate method to improve the crop yield in agricultural science.
Mahendra Kumar Trivedi,
Evaluation of Vegetative Growth Parameters in Biofield Treated Bottle Gourd (Lagenaria siceraria) and Okra (Abelmoschus esculentus), International Journal of Nutrition and Food Sciences.
Vol. 4, No. 6,
2015, pp. 688-694.
Petrovska BB (2012) Historical review of medicinal plants’ usage. Pharmacogn Rev 6: 1-5.
Decker-Walters DS, Wilkins-Ellert M, Chung SM, Staub JE (2004) Discovery and genetic assessment of wild bottle gourd [Lagenaraia siceraria (Mol.) Standley; Cucurbitaceae] from Zimbabwe. Econ Bot 58: 501-508.
Whitaker TW, Kelly JC, Pennington CW, Rands RL (1971) Men across the sea. University of Texas Press, Austin.
Sirohi PS, Sivakami N (1991) Genetic variability in cucurbits bottle gourd. Indian Hortic 36: 44-45.
Prajapati RP, Kalariya M, Parmar SK, Sheth NR (2010) Phytochemical and pharmacological review of Lagenaria sicereria. J Ayurveda Integr Med 1: 266-272.
Hammon S, Van Sloten DH (1989) Characterization and evaluation of okra. In The Use of Plant Genetic Resources. Cambridge University Press, Cambridge, UK.
Rashid MM (1999) Sabji Biggan. Rashid Publishing House, Dhaka.
Chopra RN, Nayar SL, Chopra IC (1956) Glossary of Indian Medicinal Plants, Council of Scientific & Industrial Research, New Delhi, India.
Mohan S, Devasenapathy D, Venilla C, Gill MS (2010) Pest and disease management in organic ecosystem. IPM Booklet, Tamil Nadu University, India.
Begum M (2006) Studies on seed-borne fungal diseases of okra [Abelmoschus esculentus L. (Moench)] and their management. PhD Thesis, University of Mysore, India.
Begum M, Lokesh S, Ravishankar RV, Shailaja MD, Kumar TV, et al (2005) Evaluation of certain storage conditions for okra (Abelmoschus esculentus (L.) Moench) seeds against potential fungal pathogens. Int J Agric Biol 7: 550-554.
Sances F, Flora E, Patil S, Spence A, Shinde V (2013) Impact of biofield treatment on ginseng and organic blueberry yield. Agrivita J Agric Sci 35: 22-29.
Shinde V, Sances F, Patil S, Spence A (2012) Impact of biofield treatment on growth and yield of lettuce and tomato. Aust J Basic Appl Sci 6: 100-105.
NIH, National Center for Complementary and Alternative Medicine. CAM Basics. Publication 347. [October 2, 2008]. Available at: http://nccam.nih.gov/health/whatiscam/
Movaffaghi Z, Hassanpoor M, Farsi M, Hooshmand P, Abrishami F (2006) Effects of therapeutic touch on blood hemoglobin and hematocrit level. J Holist Nurs 24: 41-48.
Olson M, Sneed N, LaVia M, Virella G, Bonadonna R, et al. (1997) Stress-induced immunosuppression and therapeutic touch. Altern Ther Health Med 3: 68-74.
Wirth DP (1990) The effect of none contact therapeutic touch on the healing rate of full thickness dermal wounds. Subtle Energies 1: 1-20.
Lenssen AW (2013) Biofield and fungicide seed treatment influences on soybean productivity, seed quality and weed community. Agricultural Journal 8: 138-143.
Nayak G, Altekar N (2015) Effect of biofield treatment on plant growth and adaptation. J Environ Health Sci 1: 1-9.
Moron MS, Depierre JW, Mannervik B (1979) Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lung and liver. Biochim Biophys Acta 582: 67-78.
Green MR, Sambrook J (2012) Molecular cloning: A laboratory manual. (3rdedn), Cold Spring Harbor, Cold Spring Harbor Laboratory Press, NY.
Borges A, Rosa MS, Recchia GH, QueirozSilva JRD, Bressan EDA, et al. (2009) CTAB methods for DNA extraction of sweet potato for microsatellite analysis. Sci Agric (Piracicaba Braz) 66: 529-534.
Welsh JW, McClelland M (1990) Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res 18: 7213-7218.
Aladjadjiyan A (2010) Influence of stationary magnetic field on lentil seeds. Int Agrophys 24: 321-324.
Yonas M, Garedew W, Debela A (2014) Multivariate analysis among okra (Abelmoschus esculentus (l.) moench) collection in South Western Ethiopia. J Plant Sci 9: 43-50.
Gemede HF, Ratta N, Haki GD, Woldegiorgis AZ, Beyene F (2015) Nutritional quality and health benefits of okra (Abelmoschus esculentus): A review. J Food Process Technol 6: 458.
Iturbe-Ormaetxe I, Escuredo PR, Arrese-Igor C, Becana M (1998) Oxidative damage in pea plants exposed to water deficit or paraquat. Plant Physiol 116: 173-181.
Grill D, Esterbauer H, Hellig K (1982) Further studies on the plants by catalase inhibitors. Plant Physiol 79: 1044-1047.
Loggini B, Scartazza A, Brugnoli E, Navari-Izzo F (1999) Antioxidative defense system, pigment composition, and photosynthetic efficiency in two wheat cultivars subjected to drought. Plant Physiol 119: 1091-1100.
Subrahmanyam GV, Sushma M, Alekya A, Neeraja CH, Harsha HSS, et al. (2011) Antidiabetic activity of Abelmoschus esculentus fruit extract. Int J Res Pharm Chem 1: 17-20.
Ngoc TH, Ngo QN, Van ATT, Phung NV (2008) Hypolipidemic effect of extracts from Abelmoschus esculentus L. (Malvaceae) on tyloxapol-induced hyperlipidemia in mice. Mahidol University Journal of Pharmaceutical Sciences 35: 42-46.
Khomsug P, Thongjaroenbuangam W, Pakdeenarong N, Suttajit M, Chantiratikul P (2010) Antioxidative activities and phenolic content from okra (Abelmoschus esculentus L.) Res J Biol Sci 5: 310-313.
Sabitha V, Ramachandran S, Naveen KR, Panneerselvam K (2012) Investigation of in vivo antioxidant property of Abelmoschus esculentus (L) moench. fruit seed and peel powders in streptozotocin-induced diabetic rats. J Ayurveda Integr Med 3: 188-193.
Nybom H, Weising K, Rotter B (2014) DNA fingerprinting in botany: Past, present, future. Investig Genet 5: 1.
Srivastava D, Khan NA, Shamim M, Yadav P, Pandey P, et al. (2014) Assessment of the genetic diversity in bottle gourd (Lagenaria siceraria [Molina] Standl.) genotypes using SDS-PAGE and RAPD Markers. Natl Acad Sci Lett 37: 155-161.
Raj M, Prasanna NKP, Peter KB (1993) Bitter gourd Momordica ssp. In: Berg BO, Kalo G (eds) Genetic improvement of vegetable crops. Pergmon Press, Oxford.
Archak S, Karihaloo JL, Jain A (2002) RAPD markers reveal narrowing genetic base of Indian tomato cultivars. Curr Sci 82: 1139-1143.
Naz A, Jamil Y, ul Haq Z, Iqbal M, Ahmad MR, et al. (2012) Enhancement in the germination, growth and yield of okra (Abelmoschus esculentus) using pre-sowing magnetic treatment of seeds. Indian J Biochem Biophys 49: 211-214.
Anand A, Nagarajan S, Verma AP, Joshi DK, Pathak PC, et al. (2012) Pre-treatment of seeds with static magnetic field ameliorates soil water stress in seedlings of maize (Zea mays L.). Indian J Biochem Biophysics 49: 63-70.
Alexander MP, Doijode SD (1995) Electromagnetic field, a novel tool to increase germination and seedling vigour of conserved onion (Allium cepa L.) and rice (Oryza sativa L.) seeds with low viability. Plant Genet Resour Newslett 104: 1-5.
Schwartz GE, Simon WL, Carmona R (2007) The energy healing experiments: Science reveals our natural power to heal. (1stedn), Atria Books.
Podlesny J (2004) The effect of magnetic stimulation of seeds on growth, development and yielding of crops. Acta Agrophysica 4: 459-473.