Monostyrene Intake in Albino Rats: Accumulation in Organs and Effects on Growth Performance and Oxidative Stress
International Journal of Nutrition and Food Sciences
Volume 5, Issue 1, January 2016, Pages: 72-79
Received: Jan. 25, 2016; Accepted: Feb. 4, 2016; Published: Feb. 26, 2016
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Authors
Mohamed G. El-Ziney, Department of Dairy Science and Technology, Faculty of Agriculture - Al Shatby, Alexandria University, Alexandria, Egypt
Hana BaAbdoulah, Department of Nutrition, King Saud University, Riyadh, Saudi Arabia
Manal S. Tawfik, Department of Food Science and Technology, Faculty of Agriculture - Al Shatby, Alexandria University, Alexandria, Egypt
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Abstract
Monostyrene (MS) residuals are daily migrating from different polystyrene shaping containers to our foods. In the present study, the distribution, accumulation and clearness rate of MS in different organs were done by one oral gavage of sub-lethal dose of 500 mg/kg bwt in male albino rates. Following MS in kidney, liver and brain indicated that the highest concentration peak was reached after 3 hr. Meanwhile, the maximum and minimum levels were detected in kidney (190.6 µg/100g) and brain (12.8 µg/100g), respectively. After 3 hr of styrene administration, the same decline rate was observed in the three organs. The clearness and metabolites rates of MS after 7 hr of administration were 93%, 76.9% and 71.1% in kidney, liver and brain, respectively. Further, this study investigated the effects of different MS at doses (0.1, 0.3 and 1.0 mg/kg) on growth performance parameters included weight gain (WG), relative liver weight, food efficiency ratio (FER), protein efficiency ratio (PER) and on oxidative stress biomarker; malondialdehyde (MDA) in the plasma of male and female albino rats after 12 weeks of feeding. All tested MS concentrations showed significant effects (P >0.05) on WG, FER and PER of either male or female rats compared to the control group. All these indices were more pronounced in both male and female that administrated MS at dose of 1.0 mg MS/kg bwt. Only in female, relative liver weight was affected significantly at doses of 0.3 and 1.0 mg/kg bwt of MS. Significant differences (P >0.05) were found among the experimental groups in malondialdehyde concentration in plasma of male and female rats; but not in nonlinear behavior. MDA concentration increased significantly (P >0.05) in female plasma compared to male at all tested doses. Styrene concentration in male liver after three months at oral administration of 1 mg/kg was 1.72 µg/100g. It is concluded that MS at tested levels had affected growth performance parameters and led to increase oxidative stress which could have significant health risks. Kidney is the main organ responsible for MS elimination. More precaution should be addressed to food packaging industry to control and monitor MS release from PS food contact materials.
Keywords
Monostyrene, Dairy Polystyrene Packages, Oxidative Stress, Malonaldialdehyde, Weight Gain, Food Efficiency, Styrene Distribution in Organs
To cite this article
Mohamed G. El-Ziney, Hana BaAbdoulah, Manal S. Tawfik, Monostyrene Intake in Albino Rats: Accumulation in Organs and Effects on Growth Performance and Oxidative Stress, International Journal of Nutrition and Food Sciences. Vol. 5, No. 1, 2016, pp. 72-79. doi: 10.11648/j.ijnfs.20160501.21
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Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
References
[1]
American Chemistry Council. The Resin Review: The Annual Statistical Report of the North American Plastic Council. [Internet]. Washington (DC): ACC. [cited 2013 Sept 5], 2013. Available from: http://www.americanchemistry.com/Jobs/EconomicStatistics/Plastics-Statistics.
[2]
Tang W., Hemm I., Eisenbrand, G, Estimation of human exposure to styrene and ethylbenzene. J Toxicology 144: 39-50, 2000.
[3]
ASTDR’s Toxicological Profiles. Toxicological Profile for Styrene. Atlanta, GA: CRC Press, 2010.
[4]
Title 21 - Food and Drugs. Code of Federal Regulations 21CFR771640, 2013.
[5]
Modern Plastic. Encyclopedia modern plastics. McGraw-Hill, 1991.
[6]
Varner S. L., Breder C. V, Headspace sampling and gas chromatographic determination of styrene migration from food-contact polystyrene cups into beverages and food stimulants. J Assoc Off Anal Chem 64: 1122-1130, 1981.
[7]
Varner S. L., Breder C. V., Fazio T, Determination of styrene migration from food-contact polymers into margarine, using Azeotropic distillation and head space gas chromatography. J Ass Offic Anal Chem Int 66: 1067–1073, 1983.
[8]
Muratak A., Avakis S., Yokoyama K, Assessment of the peripheral, central and autonomic nervous system function in styrene workers. Am J Index Medicus 20: 775–784, 1991.
[9]
Cohen T., Carlson G., Charnley G., Coggon D., Delzell E., Graham J. D, A comprehensive evaluation of the potential health risks associated with occupational and environmental exposure to styrene. J Toxicol Env Health B 5: 1–263, 2002
[10]
Benignus V. A., Geller A. M., Boyes W. K., Philip J., Bushnell J, Human neuroben avioral effects of long-term exposure to styrene: A meta-analysis. Environ Health Perspective 113: 532-538, 2005.
[11]
DHHS US. 12th Report on Carcinogens [cited 2013 Sep 5]. Available from: http://ntp.niehs.nih.gov/ntp/roc/ twelfth/roc12.pdf. 2001.
[12]
Vandepaet M. L., Bogaert M. D., Meester C. D., Noel G., Poncelet F., Mercler M, Styrene induced modifications of some fat liver enzymes involved in the activation and inactivation of xenobiotics. J Biochem Pharm 28: 1653-1659, 1978.
[13]
Institute for Local Self- Reliance, Washington. Are styrene food and beverage containers a health hazard. 1990.
[14]
Figge K, Migration of additives from plastic films into edible oils and fat stimulants. Food Cosmet Toxicol 6: 815-828, 1972.
[15]
Dowty B. J., Laseter J. L., Storet J, The tranplacental migration accumulation in blood of volatile constituents. Pediatric Res 10: 96- 101, 1976.
[16]
Polystyrene Fact sheet: Support Document. Prevention and Toxics. Available from: http://www.epa.gov/opptintr/chemfct/styresd.txt. 1994.
[17]
Davies J. T, Referred to in Twelfth Quarterly Technical Progress Report July 1, 1980 to September 30, 1980 on a Study of Indirect Food Additive Migration. FDA Contract 223-77- 2360, October 1980, (Cambridge, MA: A. D. Little, Inc.), pp. 46, 48-50, 1979.
[18]
Withey J. R., Collins P. G, Styrene monomer in foods: a limited Canadian survey. Bulletin Environ Contamination Toxicol 19: 86-94, 1978.
[19]
Tawfik M. S., Huyghebaert A, Polystyrene cups and containers: styrene migration. Food Addit Contam 15: 592-599, 1998.
[20]
WHO. Toxicological evaluation of certain food additives and food contaminants. 28th Report of the Joint FAO/WHO Expert Committee on Food Additives. WHO Food Additives Series Number19. World Health Organization, Geneva. 1984.
[21]
Biedermann M., Grob K., Morhio G, On the origin of benzene, toluene, ethylbenzene and xylene in extra virgin olive oil. Z Lebensm Unters Forsch 200: 266-272, 1995.
[22]
Tawfik M, S., BaAbdoullah H, Migration levels of monostyrene in most vulnerable foods handled and stored in polystyrene containers and their impact on the daily intake. Pak J Food Sci 24: 57-63, 2014.
[23]
Benignus V. A., Geller A. M., Boyes W. K., Philip J., Bushnell J, Human neuroben avioral effects of long-term exposure to styrene: A meta-analysis. Environ Health Perspective 113: 532-538, 2005.
[24]
Das M., Srivastava S. P., Seth P. K, Effect of styrene on glutathione content and some xenobiotic metabolizing enzymes of rat kidney. Acta Pharmacol Toxicol 52: 47-50, 1983.
[25]
Leibman K. C, Metabolism and toxicity of styrene. Environ Health Perspectives 11: 115-119, 1975.
[26]
Flanjak J., Sharrad J, Quantitative analysis of styrene monomer in foods. A limited East Australian Survey. J Sci Food Agric 35: 457-462, 1984.
[27]
Reeves P. G, Components of the AIN-93 diets as improvements in the AIN-76A diet. J Nutr 127: 808S-814S, 1997.
[28]
Esterbauer H., Cheeseman K. H, Determination of aldehydic lipid peroxidation products malondialdehyde and 4-hydroxynonenal. Method Enzymes 186: 407-421, 1990.
[29]
Berode M., Droz P. O., Boillat M. A., Guillemin M, Effect of alcohol on kinetics of styrene and its metabolites in volunteers and in workers. Appl Ind Hyg 1: 26-28, 1986.
[30]
Gold L. S., Slone T. H., Manley N. B., Bernstein L, Target organs in chronic bioassays of 533 chemical carcinogens. Environ Health Perspect 98: 233-246, 1991.
[31]
IARC. Some industrial chemicals. In: IARC Monographs, the evaluation of carcinogenic risk chemicals to human. Vol. 60, IARC, Lyon, France, 233- 320. 1994.
[32]
Vodicka P., Tuimala J., Stetina R., Kumar R., Manini P., Naccarati A., Maqstri L., Vodickov L., Kuricova M., Mutti A., Imbiani M, Cytogenetic Markers, DNA single-strand breaks, urinary metabolites and DNA repair rates in styrene-exposed lamination workers. Environ Health Persectiv 112: 867-871. 2004.
[33]
Carlson G. P, Comparison of the susceptibility of wild and CYP2E1 knockout mice to the hepatotoxic and pneumotoxic effects of styrene and styrene oxide. Toxicol Lett 150: 335-339, 2004.
[34]
Baratsch H., Malaveille C., Montesano R, Human, rat and mouse liver-mutagenicty of vinyl chloride in S. tyhimurium strains. Int J Cancer 15: 429-437, 1975.
[35]
WHO. Styrene chapter. 5: 12: 1-31, 2002.
[36]
National Cancer Institute. Carcinogenesis technical report series. Bioassay of Styrene for Possible Carcinogenicity. No. 185. 1978.
[37]
Ponomarkov V., Tomatis L, Effects of long-term oral administration of styrene to mice and rats. Scand J Work Environ Health 4: 127-135, 1978.
[38]
IARC. Summaries and Evaluations. Styrene is possibly carcinogenic to humans (Group 2B). 60: 233. CAS, No. 100-42-5. 1994.
[39]
Ottawa Styrene (Canadian Environmental Protection Act. Priority Substances List assessment report, PSL-24E), Canada Communication Group. 1993.
[40]
Katoh T., Higashi K., Lnouve N, Sub-chronic effects of styrene and styrene oxide on lipid peroxidation and the metabolism of glutathione in rat liver and brain. J Toxicol Sci 14: 1-9, 1989.
[41]
Wolf M. A., Rowe V. K., Mccollister D. D., Hollingsworth R. L., Oyen F, Toxicological studies of certain alkylated benzenes and benzene-experiments on laboratory animals. Am Arch Int Health 14: 387-398, 1956.
[42]
NTP. Pathology working group's chairperson's report. 13-Week toxicity study of styrene (CO2200B) in F344 Rats and B6C3F1 Mice by inhalation. Report dated 28 April, 1992.
[43]
Ogleznev G, Comparative characteristics of the toxic action of aqueous solution of methylostyrene and styrene. Tr Omskogo Med Inst 98: 164-174, 1963.
[44]
Cruzan G, Chronic toxicity carcinogenicity study of styrene in CD-1 mice by inhalation exposure for 104 weeks. Appl Toxicol 21: 185-198, 2001.
[45]
Lovri J., Mesi M., Macan M., Koprivanac M., Kelava M., Bradamante V, Measurement of malondialdehyde (MDA) level in rat plasma after simvastatin treatment using two different analytical methods. Periodicum Biologorum 110: 63–67, 2008.
[46]
Gray J. I, Measurement of lipid oxidation: A review. J Am Oil Chem Soc 55: 539–46, 1978.
[47]
Frankel E. N., Neff W. E, Formation of malonaldehyde from lipid oxidation product. Biochim Biophys Acta 754: 264–70, 1983.
[48]
Melton S. L, Methodology for following lipid oxidation in muscle foods. Food Technol 37: 105–9, 1983.
[49]
Anguloa J., Romera J. M., Ramirez M., Gil A, Effects of storage conditions on lipid oxidation in infant formulas based on several protein sources. J Am Oil Chem Soc 75: 1603–07, 1998.
[50]
Srivastava S. P., Das M., Seth P. K, Enhancement of lipid peroxidation in rat liver on acute exposure to acrylamide and styrene a consequence of glutathione depletion. Chem Obiol Interact 45: 373–380, 1983.
[51]
Sperlingova I., Dabrowska L., Stransky J., Cerar K. U., Tichy M, Priliminary study to preparc a reference a reference material of styrene metabolites-mandelic acid and phenylglyoxylic in human urine. Quality, Comparability and Reliability in Chemical Measarent 8: 113-116, 2004.
[52]
Radeva M., Krustev L, Studies on the effect of styrene monomer on albino rats after long-term oral administration. Khig Zdraveopaz 25: 464-468, 1982.
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