Cell Migration Induced by Native Type IV Collagen Requires PI3K/Akt2 and EGFR Activity in MDA-MB-231 Breast Cancer Cells
Cancer Research Journal
Volume 3, Issue 3, May 2015, Pages: 52-62
Received: May 6, 2015; Accepted: May 26, 2015; Published: Jun. 16, 2015
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Emmanuel Reyes-Uribe, Departamento de Biologia Celular, Cinvestav-IPN. Mexico, Mexico
Octavio Galindo-Hernandez, Departamento de Biologia Celular, Cinvestav-IPN. Mexico, Mexico
Pedro Cortes-Reynosa, Departamento de Biologia Celular, Cinvestav-IPN. Mexico, Mexico
Eduardo Perez Salazar, Departamento de Biologia Celular, Cinvestav-IPN. Mexico, Mexico
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Basement membrane (BM) is a specialized extracellular matrix (ECM) that separates epithelial cells from surrounding stroma and regulates various biological processes including morphology, growth, differentiation, adhesion and motility. Type IV collagen is the major component of BM, provides structural framework of all BMs and interacts with cell surface receptors including integrins and discoidin domain receptors (DDRs). The DDRs are receptor tyrosine kinases that get activated by collagens in their native triple-helical form and present sustained and slow activation kinetics. Particularly, DDR1 signaling mediates differentiation, immune response, migration and wound healing. However, the signal transduction pathways involved in cell migration induced by native IV collagen in breast cancer cells has been poorly studied. Here we demonstrate that native type IV collagen induces Akt2 and FAK activation through a DDR1, PI3K and epidermal growth factor receptor (EGFR)-dependent pathway in MDA-MB-231 breast cancer cells. In addition, cell migration induced by native type IV collagen requires PI3K, Akt2 and EGFR activity, whereas collagen IV also induces NFκB-DNA binding activity through a DDR1, PI3K, Akt2 and EGFR-dependent pathway. In summary, we demonstrate that migration induced by native type IV collagen requires PI3K/Akt2 and EGFR activity in MDA-MB-231 breast cancer cells.
Collagen IV, DDR1, Akt2, PI3K, EGFR, Breast Cancer
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Emmanuel Reyes-Uribe, Octavio Galindo-Hernandez, Pedro Cortes-Reynosa, Eduardo Perez Salazar, Cell Migration Induced by Native Type IV Collagen Requires PI3K/Akt2 and EGFR Activity in MDA-MB-231 Breast Cancer Cells, Cancer Research Journal. Vol. 3, No. 3, 2015, pp. 52-62. doi: 10.11648/j.crj.20150303.12
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