Organ Culture Model of Liver for the Study of Cancer Treatment for Hepatocellular Carcinoma

Torricelli Piera; Antonelli Francesco; Ferorelli Pasquale; De Martino Angelo; Shevchenko Anna; Siciliano Alberto; Beninati Simone

doi:doi:10.11648/j.crj.20160402.13

Organ Culture Model of Liver for the Study of Cancer Treatment for Hepatocellular Carcinoma

Torricelli Piera, Antonelli Francesco, Ferorelli Pasquale, De Martino Angelo, Shevchenko Anna, Siciliano Alberto, Beninati Simone

Published in Cancer Research Journal (Volume 4, Issue 2)

Received: 22 February 2016 Accepted: 1 March 2016 Published: 16 March 2016

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Abstract

The liver, the largest organ of the human body, is a multifunctional organ with various metabolic activities that plays a fundamental role in maintaining the body and in sustaining life. Although the liver has great regenerative capacity and recovery, the damage caused by chronic diseases such as cancer or viral infections can lead to permanent loss of liver function. Studies on the mechanism of liver disease, have focused on the selection of cell and tissue culture techniques, including strategies based on in vitro models. The organ culture is a promising tool for the study of liver diseases, because it can mimic the complex of the microenvironment in vivo using a three-dimensional model of human liver tissue. These models allow a better study of the specific functions of the liver. In this context, we have analyzed the development of a hepatocarcinoma, obtained by inoculating a murine hepatocarcinoma cell line, Hepa 1/A1s, in the liver of 10 mice of the strain C57BL / 6. After 20 days from the inoculation, the portion of liver invaded by the tumor was removed from the animals and cultured. A group of 5 liver explants were used as a control and other 5 explants were cultured for 4 weeks in a complete medium containing 10% Citozym, a food supplement with reported antioxidant properties. The cancer-invaded hepatic lobes, treated with Citozym, showed a clear reduction of the weight and the volume of the hepatic tumors, when compared with the control explants.

Published in	Cancer Research Journal (Volume 4, Issue 2)
DOI	10.11648/j.crj.20160402.13
Page(s)	37-42
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Food Supplements, Antioxidants, Hepatocarcinoma, Organ Cultures, Oxidative Stress

References

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APA Style

Torricelli Piera, Antonelli Francesco, Ferorelli Pasquale, De Martino Angelo, Shevchenko Anna, et al. (2016). Organ Culture Model of Liver for the Study of Cancer Treatment for Hepatocellular Carcinoma. Cancer Research Journal, 4(2), 37-42. https://doi.org/10.11648/j.crj.20160402.13

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ACS Style

Torricelli Piera; Antonelli Francesco; Ferorelli Pasquale; De Martino Angelo; Shevchenko Anna, et al. Organ Culture Model of Liver for the Study of Cancer Treatment for Hepatocellular Carcinoma. Cancer Res. J. 2016, 4(2), 37-42. doi: 10.11648/j.crj.20160402.13

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AMA Style

Torricelli Piera, Antonelli Francesco, Ferorelli Pasquale, De Martino Angelo, Shevchenko Anna, et al. Organ Culture Model of Liver for the Study of Cancer Treatment for Hepatocellular Carcinoma. Cancer Res J. 2016;4(2):37-42. doi: 10.11648/j.crj.20160402.13

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@article{10.11648/j.crj.20160402.13,
  author = {Torricelli Piera and Antonelli Francesco and Ferorelli Pasquale and De Martino Angelo and Shevchenko Anna and Siciliano Alberto and Beninati Simone},
  title = {Organ Culture Model of Liver for the Study of Cancer Treatment for Hepatocellular Carcinoma},
  journal = {Cancer Research Journal},
  volume = {4},
  number = {2},
  pages = {37-42},
  doi = {10.11648/j.crj.20160402.13},
  url = {https://doi.org/10.11648/j.crj.20160402.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.crj.20160402.13},
  abstract = {The liver, the largest organ of the human body, is a multifunctional organ with various metabolic activities that plays a fundamental role in maintaining the body and in sustaining life. Although the liver has great regenerative capacity and recovery, the damage caused by chronic diseases such as cancer or viral infections can lead to permanent loss of liver function. Studies on the mechanism of liver disease, have focused on the selection of cell and tissue culture techniques, including strategies based on in vitro models. The organ culture is a promising tool for the study of liver diseases, because it can mimic the complex of the microenvironment in vivo using a three-dimensional model of human liver tissue. These models allow a better study of the specific functions of the liver. In this context, we have analyzed the development of a hepatocarcinoma, obtained by inoculating a murine hepatocarcinoma cell line, Hepa 1/A1s, in the liver of 10 mice of the strain C57BL / 6. After 20 days from the inoculation, the portion of liver invaded by the tumor was removed from the animals and cultured. A group of 5 liver explants were used as a control and other 5 explants were cultured for 4 weeks in a complete medium containing 10% Citozym, a food supplement with reported antioxidant properties. The cancer-invaded hepatic lobes, treated with Citozym, showed a clear reduction of the weight and the volume of the hepatic tumors, when compared with the control explants.},
 year = {2016}
}

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TY  - JOUR
T1  - Organ Culture Model of Liver for the Study of Cancer Treatment for Hepatocellular Carcinoma
AU  - Torricelli Piera
AU  - Antonelli Francesco
AU  - Ferorelli Pasquale
AU  - De Martino Angelo
AU  - Shevchenko Anna
AU  - Siciliano Alberto
AU  - Beninati Simone
Y1  - 2016/03/16
PY  - 2016
N1  - https://doi.org/10.11648/j.crj.20160402.13
DO  - 10.11648/j.crj.20160402.13
T2  - Cancer Research Journal
JF  - Cancer Research Journal
JO  - Cancer Research Journal
SP  - 37
EP  - 42
PB  - Science Publishing Group
SN  - 2330-8214
UR  - https://doi.org/10.11648/j.crj.20160402.13
AB  - The liver, the largest organ of the human body, is a multifunctional organ with various metabolic activities that plays a fundamental role in maintaining the body and in sustaining life. Although the liver has great regenerative capacity and recovery, the damage caused by chronic diseases such as cancer or viral infections can lead to permanent loss of liver function. Studies on the mechanism of liver disease, have focused on the selection of cell and tissue culture techniques, including strategies based on in vitro models. The organ culture is a promising tool for the study of liver diseases, because it can mimic the complex of the microenvironment in vivo using a three-dimensional model of human liver tissue. These models allow a better study of the specific functions of the liver. In this context, we have analyzed the development of a hepatocarcinoma, obtained by inoculating a murine hepatocarcinoma cell line, Hepa 1/A1s, in the liver of 10 mice of the strain C57BL / 6. After 20 days from the inoculation, the portion of liver invaded by the tumor was removed from the animals and cultured. A group of 5 liver explants were used as a control and other 5 explants were cultured for 4 weeks in a complete medium containing 10% Citozym, a food supplement with reported antioxidant properties. The cancer-invaded hepatic lobes, treated with Citozym, showed a clear reduction of the weight and the volume of the hepatic tumors, when compared with the control explants.
VL  - 4
IS  - 2
ER  -

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Author Information

Torricelli Piera

Department SPES, University of Molise, Campobasso, Italy
Antonelli Francesco

Department of Biology, University of Tor Vergata, Rome, Italy
Ferorelli Pasquale

Department of Biology, University of Tor Vergata, Rome, Italy
De Martino Angelo

Department of Biology, University of Tor Vergata, Rome, Italy
Shevchenko Anna

People’s Friendship University of Russia, Moscow, Russia
Siciliano Alberto

Department of Biology, University of Tor Vergata, Rome, Italy
Beninati Simone

Department of Biology, University of Tor Vergata, Rome, Italy

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APA Style

Torricelli Piera, Antonelli Francesco, Ferorelli Pasquale, De Martino Angelo, Shevchenko Anna, et al. (2016). Organ Culture Model of Liver for the Study of Cancer Treatment for Hepatocellular Carcinoma. Cancer Research Journal, 4(2), 37-42. https://doi.org/10.11648/j.crj.20160402.13

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ACS Style

Torricelli Piera; Antonelli Francesco; Ferorelli Pasquale; De Martino Angelo; Shevchenko Anna, et al. Organ Culture Model of Liver for the Study of Cancer Treatment for Hepatocellular Carcinoma. Cancer Res. J. 2016, 4(2), 37-42. doi: 10.11648/j.crj.20160402.13

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AMA Style

Torricelli Piera, Antonelli Francesco, Ferorelli Pasquale, De Martino Angelo, Shevchenko Anna, et al. Organ Culture Model of Liver for the Study of Cancer Treatment for Hepatocellular Carcinoma. Cancer Res J. 2016;4(2):37-42. doi: 10.11648/j.crj.20160402.13

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@article{10.11648/j.crj.20160402.13,
  author = {Torricelli Piera and Antonelli Francesco and Ferorelli Pasquale and De Martino Angelo and Shevchenko Anna and Siciliano Alberto and Beninati Simone},
  title = {Organ Culture Model of Liver for the Study of Cancer Treatment for Hepatocellular Carcinoma},
  journal = {Cancer Research Journal},
  volume = {4},
  number = {2},
  pages = {37-42},
  doi = {10.11648/j.crj.20160402.13},
  url = {https://doi.org/10.11648/j.crj.20160402.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.crj.20160402.13},
  abstract = {The liver, the largest organ of the human body, is a multifunctional organ with various metabolic activities that plays a fundamental role in maintaining the body and in sustaining life. Although the liver has great regenerative capacity and recovery, the damage caused by chronic diseases such as cancer or viral infections can lead to permanent loss of liver function. Studies on the mechanism of liver disease, have focused on the selection of cell and tissue culture techniques, including strategies based on in vitro models. The organ culture is a promising tool for the study of liver diseases, because it can mimic the complex of the microenvironment in vivo using a three-dimensional model of human liver tissue. These models allow a better study of the specific functions of the liver. In this context, we have analyzed the development of a hepatocarcinoma, obtained by inoculating a murine hepatocarcinoma cell line, Hepa 1/A1s, in the liver of 10 mice of the strain C57BL / 6. After 20 days from the inoculation, the portion of liver invaded by the tumor was removed from the animals and cultured. A group of 5 liver explants were used as a control and other 5 explants were cultured for 4 weeks in a complete medium containing 10% Citozym, a food supplement with reported antioxidant properties. The cancer-invaded hepatic lobes, treated with Citozym, showed a clear reduction of the weight and the volume of the hepatic tumors, when compared with the control explants.},
 year = {2016}
}

Copy | Download

TY  - JOUR
T1  - Organ Culture Model of Liver for the Study of Cancer Treatment for Hepatocellular Carcinoma
AU  - Torricelli Piera
AU  - Antonelli Francesco
AU  - Ferorelli Pasquale
AU  - De Martino Angelo
AU  - Shevchenko Anna
AU  - Siciliano Alberto
AU  - Beninati Simone
Y1  - 2016/03/16
PY  - 2016
N1  - https://doi.org/10.11648/j.crj.20160402.13
DO  - 10.11648/j.crj.20160402.13
T2  - Cancer Research Journal
JF  - Cancer Research Journal
JO  - Cancer Research Journal
SP  - 37
EP  - 42
PB  - Science Publishing Group
SN  - 2330-8214
UR  - https://doi.org/10.11648/j.crj.20160402.13
AB  - The liver, the largest organ of the human body, is a multifunctional organ with various metabolic activities that plays a fundamental role in maintaining the body and in sustaining life. Although the liver has great regenerative capacity and recovery, the damage caused by chronic diseases such as cancer or viral infections can lead to permanent loss of liver function. Studies on the mechanism of liver disease, have focused on the selection of cell and tissue culture techniques, including strategies based on in vitro models. The organ culture is a promising tool for the study of liver diseases, because it can mimic the complex of the microenvironment in vivo using a three-dimensional model of human liver tissue. These models allow a better study of the specific functions of the liver. In this context, we have analyzed the development of a hepatocarcinoma, obtained by inoculating a murine hepatocarcinoma cell line, Hepa 1/A1s, in the liver of 10 mice of the strain C57BL / 6. After 20 days from the inoculation, the portion of liver invaded by the tumor was removed from the animals and cultured. A group of 5 liver explants were used as a control and other 5 explants were cultured for 4 weeks in a complete medium containing 10% Citozym, a food supplement with reported antioxidant properties. The cancer-invaded hepatic lobes, treated with Citozym, showed a clear reduction of the weight and the volume of the hepatic tumors, when compared with the control explants.
VL  - 4
IS  - 2
ER  -

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