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The mtlD Gene-overexpressed Transgenic Wheat Tolerates Salt Stress Through Accumulation of Mannitol and Sugars

Received: 17 September 2016    Accepted: 12 October 2016    Published: 31 October 2016
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Abstract

The mtlD gene-contained transgenic wheat has established the role of mannitol and sugars accumulation in alleviating the abiotic stresses, including salinity. This study was conducted to determine whether the 85 mM NaCl-salinity could be tolerated by wheat (genotype 235/3) plants of which seeds were transformed with mtlD gene (from Escherichia coli). The effects of mtlD gene transformation into wheat seeds on growth traits, physio-biochemical attributes, and yield and its quality of transgenic wheat genotype were investigated compared to non-transgenic wheat genotype under 85 mM NaCl-salinity. Results showed that mtlD gene-contained transgenic plants had improved salt tolerance over non-transgenics, showing by better growth traits (i.e., number of leaves and leaf area per plant, root system size and plant dry weights), physio-biochemical attributes (i.e., levels of leaf chlorophylls, shoot free proline, total soluble sugars, soluble sugar fractions and mannitol, activities of enzymatic and non-enzymatic antioxidants, and contents of nutrient elements), yield (i.e., number of spikes and grain weight per plant, and 1000-grain weight) and yield quality (i.e., grain contents of starch, protein and soluble sugars). The mtlD gene transformation into wheat seeds appears to a better strategy to increase salt tolerance of plants through increased performance of mannitol and sugar accumulation, showing more of their salt stress-protecting role. The best performing mtlD transgenics could be incorporated in a breeding program to accumulate transgenes for stress tolerance in elite wheat genotypes in a step to commercialize these transgenics with the proper level of salt tolerance.

DOI 10.11648/j.plant.20160406.15
Published in Plant (Volume 4, Issue 6, November 2016)
Page(s) 78-90
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This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Physio-Biochemical Attributes, Salt Stress, Transgenic Wheat, Yield and Its Quality

References
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Author Information
  • Botany Department, Faculty of Agriculture, Fayoum University, Fayoum, Egypt

  • Agricultural Genetic Engineering Research Institute (AGERI), Agriculture Research Center (ARC), Giza, Egypt

  • Botany Department, Faculty of Agriculture, Fayoum University, Fayoum, Egypt

  • Biology Department, Faculty of Sciences, Albaha University, Albaha, Saudi Arabia

  • Department of Water Relations and Field Irrigation, National Research Centre, Dokki, Cairo, Egypt

  • Botany Department, Faculty of Agriculture, Fayoum University, Fayoum, Egypt

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    Mohamed Ahmed Seif El-Yazal, Hala Fawzi Eissa, Safia Mahmoud Abd El-Mageed Ahmed, Saad Mohamed Howladar, Safi-naz Sabet Zaki, et al. (2016). The mtlD Gene-overexpressed Transgenic Wheat Tolerates Salt Stress Through Accumulation of Mannitol and Sugars. Plant, 4(6), 78-90. https://doi.org/10.11648/j.plant.20160406.15

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    Mohamed Ahmed Seif El-Yazal; Hala Fawzi Eissa; Safia Mahmoud Abd El-Mageed Ahmed; Saad Mohamed Howladar; Safi-naz Sabet Zaki, et al. The mtlD Gene-overexpressed Transgenic Wheat Tolerates Salt Stress Through Accumulation of Mannitol and Sugars. Plant. 2016, 4(6), 78-90. doi: 10.11648/j.plant.20160406.15

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    Mohamed Ahmed Seif El-Yazal, Hala Fawzi Eissa, Safia Mahmoud Abd El-Mageed Ahmed, Saad Mohamed Howladar, Safi-naz Sabet Zaki, et al. The mtlD Gene-overexpressed Transgenic Wheat Tolerates Salt Stress Through Accumulation of Mannitol and Sugars. Plant. 2016;4(6):78-90. doi: 10.11648/j.plant.20160406.15

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  • @article{10.11648/j.plant.20160406.15,
      author = {Mohamed Ahmed Seif El-Yazal and Hala Fawzi Eissa and Safia Mahmoud Abd El-Mageed Ahmed and Saad Mohamed Howladar and Safi-naz Sabet Zaki and Mostafa Mohamed Rady},
      title = {The mtlD Gene-overexpressed Transgenic Wheat Tolerates Salt Stress Through Accumulation of Mannitol and Sugars},
      journal = {Plant},
      volume = {4},
      number = {6},
      pages = {78-90},
      doi = {10.11648/j.plant.20160406.15},
      url = {https://doi.org/10.11648/j.plant.20160406.15},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.plant.20160406.15},
      abstract = {The mtlD gene-contained transgenic wheat has established the role of mannitol and sugars accumulation in alleviating the abiotic stresses, including salinity. This study was conducted to determine whether the 85 mM NaCl-salinity could be tolerated by wheat (genotype 235/3) plants of which seeds were transformed with mtlD gene (from Escherichia coli). The effects of mtlD gene transformation into wheat seeds on growth traits, physio-biochemical attributes, and yield and its quality of transgenic wheat genotype were investigated compared to non-transgenic wheat genotype under 85 mM NaCl-salinity. Results showed that mtlD gene-contained transgenic plants had improved salt tolerance over non-transgenics, showing by better growth traits (i.e., number of leaves and leaf area per plant, root system size and plant dry weights), physio-biochemical attributes (i.e., levels of leaf chlorophylls, shoot free proline, total soluble sugars, soluble sugar fractions and mannitol, activities of enzymatic and non-enzymatic antioxidants, and contents of nutrient elements), yield (i.e., number of spikes and grain weight per plant, and 1000-grain weight) and yield quality (i.e., grain contents of starch, protein and soluble sugars). The mtlD gene transformation into wheat seeds appears to a better strategy to increase salt tolerance of plants through increased performance of mannitol and sugar accumulation, showing more of their salt stress-protecting role. The best performing mtlD transgenics could be incorporated in a breeding program to accumulate transgenes for stress tolerance in elite wheat genotypes in a step to commercialize these transgenics with the proper level of salt tolerance.},
     year = {2016}
    }
    

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    T1  - The mtlD Gene-overexpressed Transgenic Wheat Tolerates Salt Stress Through Accumulation of Mannitol and Sugars
    AU  - Mohamed Ahmed Seif El-Yazal
    AU  - Hala Fawzi Eissa
    AU  - Safia Mahmoud Abd El-Mageed Ahmed
    AU  - Saad Mohamed Howladar
    AU  - Safi-naz Sabet Zaki
    AU  - Mostafa Mohamed Rady
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    T2  - Plant
    JF  - Plant
    JO  - Plant
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    EP  - 90
    PB  - Science Publishing Group
    SN  - 2331-0677
    UR  - https://doi.org/10.11648/j.plant.20160406.15
    AB  - The mtlD gene-contained transgenic wheat has established the role of mannitol and sugars accumulation in alleviating the abiotic stresses, including salinity. This study was conducted to determine whether the 85 mM NaCl-salinity could be tolerated by wheat (genotype 235/3) plants of which seeds were transformed with mtlD gene (from Escherichia coli). The effects of mtlD gene transformation into wheat seeds on growth traits, physio-biochemical attributes, and yield and its quality of transgenic wheat genotype were investigated compared to non-transgenic wheat genotype under 85 mM NaCl-salinity. Results showed that mtlD gene-contained transgenic plants had improved salt tolerance over non-transgenics, showing by better growth traits (i.e., number of leaves and leaf area per plant, root system size and plant dry weights), physio-biochemical attributes (i.e., levels of leaf chlorophylls, shoot free proline, total soluble sugars, soluble sugar fractions and mannitol, activities of enzymatic and non-enzymatic antioxidants, and contents of nutrient elements), yield (i.e., number of spikes and grain weight per plant, and 1000-grain weight) and yield quality (i.e., grain contents of starch, protein and soluble sugars). The mtlD gene transformation into wheat seeds appears to a better strategy to increase salt tolerance of plants through increased performance of mannitol and sugar accumulation, showing more of their salt stress-protecting role. The best performing mtlD transgenics could be incorporated in a breeding program to accumulate transgenes for stress tolerance in elite wheat genotypes in a step to commercialize these transgenics with the proper level of salt tolerance.
    VL  - 4
    IS  - 6
    ER  - 

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