Effects of a Single Dose of Ethanol on Survival Rate and Angiogenesis of Chick Embryo
Animal and Veterinary Sciences
Volume 3, Issue 1, January 2015, Pages: 8-11
Received: Sep. 5, 2014; Accepted: Dec. 3, 2014; Published: Jan. 20, 2015
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Authors
Zulifqar Ali Laghari, Department of Physiology, University of Sindh, Jamshoro, Sindh, Pakistan
Ayaz Ali Samo, Department of Physiology, University of Sindh, Jamshoro, Sindh, Pakistan
Baradi Waryani, Department of Fresh Water Biology and fisheries, University of Sindh, Jamshoro, Sindh, Pakistan
Zameer Ali Palh, Department of Fresh Water Biology and fisheries, University of Sindh, Jamshoro, Sindh, Pakistan
Khalid Hussain Lashari, Department of Fresh Water Biology and fisheries, University of Sindh, Jamshoro, Sindh, Pakistan
Ghulam Murtaza Mastoi, Centre for Environmental Sciences, University of Sindh, Jamshoro, Sindh, Pakistan
Gulshan Ara Sahato, Department of Fresh Water Biology and fisheries, University of Sindh, Jamshoro, Sindh, Pakistan
Tahira Jabeen Ursani, Department of Zoology, University of Sindh, Jamshoro, Sindh, Pakistan
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Abstract
The developing Chick has been widely used as a model organism for research studies in developmental biology. Various aspects of ethanol (EtOH) toxicity during embryonic development have been documented in experimental studies. The studies on chick embryo address the effects of EtOH on craniofacial abnormalities and angiogenesis. The purpose of this study was to assess the effects of a single dose of ethanol on survival rate and angiogenesis of chick embryos during early stages of development. Chick eggs were incubated at 37ºC in the humidified incubator, after 72 hours of incubation these eggs were removed from incubator, and treated with a single doses of 1%, 5%, 10%, 13% and 15% EtOH. The effects on survival rate and angiogenesis were recorded on ethanol treated chick embryos. The survival rate was slightly reduced with 1% and 5% EtOH, however with increasing dose of 10% the survival rate was reduced to 64% and at 15% EtOH complete death was observed. Our results also indicate that treatment of EtOH with 1% and 5% did not have any obvious effects on vessels formation in comparison with the BSS treated embryos. However, treatment of chick embryo with 10%, 13% and 15% EtOH severely inhibited the blood vessels formation.
Keywords
Chick Embryo, Angiogenesis, EtOH
To cite this article
Zulifqar Ali Laghari, Ayaz Ali Samo, Baradi Waryani, Zameer Ali Palh, Khalid Hussain Lashari, Ghulam Murtaza Mastoi, Gulshan Ara Sahato, Tahira Jabeen Ursani, Effects of a Single Dose of Ethanol on Survival Rate and Angiogenesis of Chick Embryo, Animal and Veterinary Sciences. Vol. 3, No. 1, 2015, pp. 8-11. doi: 10.11648/j.avs.20150301.12
References
[1]
Biau, S., S. Bayle, et al. (2007). "The chick embryo: an animal model for detection of the effects of hormonal compounds." Anal Bioanal Chem 387(4): 1397-403.
[2]
Bilotta, J., J. A. Barnett, et al. (2004). "Ethanol exposure alters zebrafish development: a novel model of fetal alcohol syndrome." Neurotoxicol Teratol 26(6): 737-43.
[3]
Chaudhuri, J. D. (2004). "Effect of a single dose of ethanol on developing skeletal muscle of chick embryos." Alcohol 34(2-3): 279-83.
[4]
Cimpean, A. M., D. Ribatti, et al. (2008). "The chick embryo chorioallantoic membrane as a model to study tumor metastasis." Angiogenesis 11(4): 311-9.
[5]
Deryugina, E. I. and J. P. Quigley (2008). "Chapter 2. Chick embryo chorioallantoic membrane models to quantify angiogenesis induced by inflammatory and tumor cells or purified effector molecules." Methods Enzymol 444: 21-41.
[6]
Eckstein, L. W., I. A. Shibley, Jr., et al. (1997). "Changes in brain glucose levels and glucose transporter protein isoforms in alcohol- or nicotine-treated chick embryos." Brain Res Dev Brain Res 103(1): 59-65.
[7]
Garic-Stankovic, A., M. R. Hernandez, et al. (2005). "Ethanol triggers neural crest apoptosis through the selective activation of a pertussis toxin-sensitive G protein and a phospholipase Cbeta-dependent Ca2+ transient." Alcohol Clin Exp Res 29(7): 1237-46
[8]
Ginter, E. and V. Simko (2008). "Ethanol and cardiovascular diseases: epidemiological, biochemical and clinical aspects." Bratisl Lek Listy 109(12): 590-4.
[9]
Gu, J. W., A. P. Bailey, et al. (2005). "Ethanol stimulates tumor progression and expression of vascular endothelial growth factor in chick embryos." Cancer 103(2): 422-31.
[10]
Gu, J. W., J. Elam, et al. (2001). "Moderate levels of ethanol induce expression of vascular endothelial growth factor and stimulate angiogenesis." Am J Physiol Regul Integr Comp Physiol 281(1): R365-72
[11]
He, R. R., Y. Li, et al. (2013). "A new oxidative stress model, 2,2-azobis(2-amidinopropane) dihydrochloride induces cardiovascular damages in chicken embryo." PLoS One 8(3): e57732.
[12]
Kamran, K., M. Y. Khan, et al. (2011). "Teratogenic effects of ethanol vapour exposure on chick embryos." J Pak Med Assoc 61(4): 328-31.
[13]
Kamran, K., M. Y. Khan, et al. (2013). "Ethanol vapour induced dilated cardiomyopathy in chick embryos." J Pak Med Assoc 63(9): 1084-8.
[14]
Karunamuni, G., S. Gu, et al. (2014). "Ethanol exposure alters early cardiac function in the looping heart: a mechanism for congenital heart defects?" Am J Physiol Heart Circ Physiol 306(3): H414-21.
[15]
Kennelly, K., D. Brennan, et al. (2011). "Histological characterisation of the ethanol-induced microphthalmia phenotype in a chick embryo model system." Reprod Toxicol 32(2): 227-34.
[16]
Lange, S., K. Shield, et al. (2013). "Prevalence of fetal alcohol spectrum disorders in child care settings: a meta-analysis." Pediatrics 132(4): e980-95.
[17]
Nakatsuji, N. (1983). "Craniofacial malformation in Xenopus laevis tadpoles caused by the exposure of early embryos to ethanol." Teratology 28(2): 299-305.
[18]
Nakatsuji, N. and K. E. Johnson (1984). "Effects of ethanol on the primitive streak stage mouse embryo." Teratology 29(3): 369-75.
[19]
Popova, S., S. Lange, et al. (2011). "Fetal alcohol spectrum disorder prevalence estimates in correctional systems: a systematic literature review." Can J Public Health 102(5): 336-40.
[20]
Pourquie, O. (2004). "The chick embryo: a leading model in somitogenesis studies." Mech Dev 121(9): 1069-79.
[21]
Radek, K. A., E. J. Kovacs, et al. (2008). "Acute ethanol exposure disrupts VEGF receptor cell signaling in endothelial cells." Am J Physiol Heart Circ Physiol 295(1): H174-84.
[22]
Radek, K. A., A. M. Matthies, et al. (2005). "Acute ethanol exposure impairs angiogenesis and the proliferative phase of wound healing." Am J Physiol Heart Circ Physiol 289(3): H1084-90.
[23]
Ribatti, D. (2008). "Chick embryo chorioallantoic membrane as a useful tool to study angiogenesis." Int Rev Cell Mol Biol 270: 181-224.
[24]
Ribatti, D. (2012). "Chicken chorioallantoic membrane angiogenesis model." Methods Mol Biol 843: 47-57.
[25]
Ribatti, D., A. Gualandris, et al. (1997). "New model for the study of angiogenesis and antiangiogenesis in the chick embryo chorioallantoic membrane: the gelatin sponge/chorioallantoic membrane assay." J Vasc Res 34(6): 455-63.
[26]
Richardson, M. and G. Singh (2003). "Observations on the use of the avian chorioallantoic membrane (CAM) model in investigations into angiogenesis." Curr Drug Targets Cardiovasc Haematol Disord 3(2): 155-85.
[27]
Rosenbruch, M. and A. Holst (1990). "The chick embryo yolk-sac blood vessel system as an experimental model for irritation and inflammation." Toxicol In Vitro 4(4-5): 327-31.
[28]
Smith, S. M. (1997). "Alcohol-induced cell death in the embryo." Alcohol Health Res World 21(4): 287-97.
[29]
Smith, S. M. (2008). "The avian embryo in fetal alcohol research." Methods Mol Biol 447: 75-84.
[30]
Spies, C. D., M. Sander, et al. (2001). "Effects of alcohol on the heart." Curr Opin Crit Care 7(5): 337-43.
[31]
Tay, S. L., P. W. Heng, et al. (2012). "The chick chorioallantoic membrane imaging method as a platform to evaluate vasoactivity and assess irritancy of compounds." J Pharm Pharmacol 64(8): 1128-37.
[32]
Tufan, A. C., G. Abban, et al. (2007). "The effect of in ovo ethanol exposure on retina and optic nerve in a chick embryo model system." Reprod Toxicol 23(1): 75-82.
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