Animal and Veterinary Sciences

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Detection of Toxoplasma gondii from Native Cattle in Southwest of Iran

Received: 03 February 2015    Accepted: 11 February 2015    Published: 12 June 2015
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Abstract

Infections by protozoan parasite Toxoplasma gondii are prevalent worldwide in animals and human. T. gondii is the causative agent of toxoplasmosis, one of the most prevalent parasitic infections to humans and domestic animals. If first be during pregnancy, T. gondii may be transferred vertically by tachyzoites that are passed to the embryo via the placenta. T. gondii may be transmitted horizontally in three phases of the life cycle, ingesting infectious oocysts from the environment or tissue cysts or tachyzoites which are contained in gastrointestinal of many different animals. Transmission may also occur via tachyzoites contained in blood products, texture transplants or non-pasteurized milk. Like rest of the world toxoplasmosis is prevalent in Iran. The present study aimed to determine T. gondii isolates from native cattle in Chaharmahal Va Bakhtiari province located in south west of Iran by molecular methods. In this study, 155 blood samples were collected from native cattle. Genomic DNA was extracted using DNA extraction Kit (Cinna Gen, Iran) according to the manufacturer protocol and PCR was performed using specific primers (ITS-F and ITS-R). Sixteen (6.95%) cattle were positive to T. gondii infection. The positive control samples showed the excepted amplification product specific for T. gondii (171 bp). Although the present results showed relatively low prevalence of T.gondii infection in Chaharmahal Va Bakhtiari native cattle, control and eradication programs seem to be still necessary to prevent the prevalence of this infection factor and economic losses.

DOI 10.11648/j.avs.20150304.11
Published in Animal and Veterinary Sciences (Volume 3, Issue 4, July 2015)
Page(s) 102-105
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Toxoplasma gondii, Toxoplasmosis, Native Cattle, PCR, Iran

References
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Author Information
  • Biotechnology Research Center, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran

  • Biotechnology Research Center, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran

  • Biotechnology Research Center, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran

  • Biotechnology Research Center, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran

Cite This Article
  • APA Style

    Asghar Arshi, Esmaeil Mahmoudi, Mohammad-Saeid Jami, Abbas Doosti. (2015). Detection of Toxoplasma gondii from Native Cattle in Southwest of Iran. Animal and Veterinary Sciences, 3(4), 102-105. https://doi.org/10.11648/j.avs.20150304.11

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    ACS Style

    Asghar Arshi; Esmaeil Mahmoudi; Mohammad-Saeid Jami; Abbas Doosti. Detection of Toxoplasma gondii from Native Cattle in Southwest of Iran. Anim. Vet. Sci. 2015, 3(4), 102-105. doi: 10.11648/j.avs.20150304.11

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    AMA Style

    Asghar Arshi, Esmaeil Mahmoudi, Mohammad-Saeid Jami, Abbas Doosti. Detection of Toxoplasma gondii from Native Cattle in Southwest of Iran. Anim Vet Sci. 2015;3(4):102-105. doi: 10.11648/j.avs.20150304.11

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  • @article{10.11648/j.avs.20150304.11,
      author = {Asghar Arshi and Esmaeil Mahmoudi and Mohammad-Saeid Jami and Abbas Doosti},
      title = {Detection of Toxoplasma gondii from Native Cattle in Southwest of Iran},
      journal = {Animal and Veterinary Sciences},
      volume = {3},
      number = {4},
      pages = {102-105},
      doi = {10.11648/j.avs.20150304.11},
      url = {https://doi.org/10.11648/j.avs.20150304.11},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.avs.20150304.11},
      abstract = {Infections by protozoan parasite Toxoplasma gondii are prevalent worldwide in animals and human. T. gondii is the causative agent of toxoplasmosis, one of the most prevalent parasitic infections to humans and domestic animals. If first be during pregnancy, T. gondii may be transferred vertically by tachyzoites that are passed to the embryo via the placenta. T. gondii may be transmitted horizontally in three phases of the life cycle, ingesting infectious oocysts from the environment or tissue cysts or tachyzoites which are contained in gastrointestinal of many different animals. Transmission may also occur via tachyzoites contained in blood products, texture transplants or non-pasteurized milk. Like rest of the world toxoplasmosis is prevalent in Iran. The present study aimed to determine T. gondii isolates from native cattle in Chaharmahal Va Bakhtiari province located in south west of Iran by molecular methods. In this study, 155 blood samples were collected from native cattle. Genomic DNA was extracted using DNA extraction Kit (Cinna Gen, Iran) according to the manufacturer protocol and PCR was performed using specific primers (ITS-F and ITS-R). Sixteen (6.95%) cattle were positive to T. gondii infection. The positive control samples showed the excepted amplification product specific for T. gondii (171 bp). Although the present results showed relatively low prevalence of T.gondii infection in Chaharmahal Va Bakhtiari native cattle, control and eradication programs seem to be still necessary to prevent the prevalence of this infection factor and economic losses.},
     year = {2015}
    }
    

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  • TY  - JOUR
    T1  - Detection of Toxoplasma gondii from Native Cattle in Southwest of Iran
    AU  - Asghar Arshi
    AU  - Esmaeil Mahmoudi
    AU  - Mohammad-Saeid Jami
    AU  - Abbas Doosti
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    JF  - Animal and Veterinary Sciences
    JO  - Animal and Veterinary Sciences
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    EP  - 105
    PB  - Science Publishing Group
    SN  - 2328-5850
    UR  - https://doi.org/10.11648/j.avs.20150304.11
    AB  - Infections by protozoan parasite Toxoplasma gondii are prevalent worldwide in animals and human. T. gondii is the causative agent of toxoplasmosis, one of the most prevalent parasitic infections to humans and domestic animals. If first be during pregnancy, T. gondii may be transferred vertically by tachyzoites that are passed to the embryo via the placenta. T. gondii may be transmitted horizontally in three phases of the life cycle, ingesting infectious oocysts from the environment or tissue cysts or tachyzoites which are contained in gastrointestinal of many different animals. Transmission may also occur via tachyzoites contained in blood products, texture transplants or non-pasteurized milk. Like rest of the world toxoplasmosis is prevalent in Iran. The present study aimed to determine T. gondii isolates from native cattle in Chaharmahal Va Bakhtiari province located in south west of Iran by molecular methods. In this study, 155 blood samples were collected from native cattle. Genomic DNA was extracted using DNA extraction Kit (Cinna Gen, Iran) according to the manufacturer protocol and PCR was performed using specific primers (ITS-F and ITS-R). Sixteen (6.95%) cattle were positive to T. gondii infection. The positive control samples showed the excepted amplification product specific for T. gondii (171 bp). Although the present results showed relatively low prevalence of T.gondii infection in Chaharmahal Va Bakhtiari native cattle, control and eradication programs seem to be still necessary to prevent the prevalence of this infection factor and economic losses.
    VL  - 3
    IS  - 4
    ER  - 

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