Animal and Veterinary Sciences

| Peer-Reviewed |

Anti-coccidial Activity of Moringa Oleifera Plant

Received: 03 May 2016    Accepted:     Published: 04 May 2016
Views:       Downloads:

Share This Article

Abstract

Moringa Oleifera leaves were screened for its toxicological effect and some pharmacological activities in broiler chicken. Toxicological effect was studied using upgrading oral doses of 75% ethanolic extract in different groups of mice with monitoring the toxicological symptoms and mortality rate. The anti-coccidal activity of Moringa Oleifera powdered leaves for prevention and its 75% ethanolic extract for treatment of coccidiosis were studied in broilers. The obtained result proved that there were no toxicological symptoms or mortalities obtained till oral administration of 6000 mg/kg b. wt. Inclusion of Moringa olifera powder (0.5 and 1%) to broiler's feed were very efficacious and equal to diclazuril (1ppm) in preventing symptoms of coccidiosis associated with experimental infection with mixed Eimeria species. The anti-coccidal activity of Moringa Oleifera powdered leaves and its 75% ethanolic extract were studied on one-day old chicks. The experimental infected chicks with mixed Eimeria species induced severe coccidiosis in non medicated chicks and led to high mortality reached to (26.76%). Addition of 200 and 400ppm of Moringa Oleifera ethanolic extract induced marked curative effect of cocci diosis manifested by reduced mortality percent and improved PM lesion to be 13.3 and 10% respectively compared to 13.3% for group treated with the reference drug (25ppm) toltrazuril.

DOI 10.11648/j.avs.20160402.12
Published in Animal and Veterinary Sciences (Volume 4, Issue 2, March 2016)
Page(s) 19-25
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Moringa Oleifera- prophylactic and treatment - Coccidiosis

References
[1] Allen, P. C., Danforth, H. D. & Augustine, P. C. (1998): Dietary modulation of avian coccidiosis. Interenational Journal of Parasitology 28, 1131–1140.
[2] Chapman, H. D. (1997): “Biochemical, genetic and applied aspects of drug resistance in Eimeria parasites of the fowl,” Avian Pathology 26, 221–244.
[3] Youn, H. J. and Noh, J. W. 2001. Screening of the anticoccidial effects of herb extracts against Eimeria tenella. Veterinary Parasitology 96, 257–263.
[4] Mishra, G.; Singh, P.; Verma, R.; Kumar, S.; Saurabh, S.; Jha, K. & Khosa, R. L.(2011): Traditional Uses, Phytochemistry and Pharmacological Properties of Moringa Oleifera plant: An Overview. Scholars Research Library. Der Pharmacia Lettre 3(2), 141-164.
[5] Farooq, A.; Sajid, L.; Muhammad, A. & Anwarul-Hassan, G. (2007): Moringa Oleifera : a food plant with multiple medicinal uses. PhytotherRes, 21, 17–25.
[6] Ola-Fadunsin, S. D. & Ademola, I. O. (2013): Direct effects of Moringa Oleifera Lam (Moringaceae) acetone leaf extract on broiler chickens naturally infected with Eimeria species. Trop Anim Health Prod.
[7] Singh M K, Paul J, De T, Chakraborti T. (2015): Bioactivity guided fractionation of Moringa Oleifera Lam. flower targeting Leishmania donovani. Indian J Exp Biol.; 53(11): 747-52.
[8] Handa, S. S. (2008): An Overview of Extraction Techniques for Medicinal and Aromatic Plants. In Handa, S. S.; Khanuja, S. P. S.; Longo, G. & Rakesh, D. V., Extraction Technologies for Medicinal and Aromatic Plants, p.p 26. International Centre for Science and High Technology, Italy.
[9] -Kerber, G. (1941): Pharmakologische Methoden Zur Auffindung Von Arzneimitten und Gifter und Analyse ihrer Wirkungsweise Vor. Dr. Med. Leopold Ther.Wissenschaftliche verlage Gerlarge Gesse Gesellschaft. M. B. H.
[10] Long, P. L. (1971): Maintenance of intestinal protozoa in-vivo with particular reference to Eimeria and Histomonas. In Isolation and Maintenance of Parasites in-vivo, 9thSymposium of the British Society for Parasitology, 65–75. Oxford: Blackwell.
[11] Norton, C. C., and L. P. Joyner (1968): The freeze preservation of coccidia. Res Vet Sci 9:598–600.
[12] Long, P. L.; Joyner, L. P. & Millard, B. J. (1976): A guide to laboratory techniques used in the study and diagnosis of Av.coccidisis. Folia Vet. Lat., (6), 201-217.
[13] Davis, L. R. (1973): Techniques. In The Coccidia, ed. D. M. Hammond and P. L. Long, 411–58. Baltimore, MD: Univ. Park Press.
[14] Johnson, J. E. & Reid, W. M. (1970): Anticoccidial drugs: Lesion scoring technique in battery and floor pen experiment with chickens. Exprel.Parasitol., 28,30-36.
[15] Hodgson, J. N. (1970): Coccidiosis: Oocysts counting technique for coccidiostat evaluation. Exprel.parasitol, 28, 99-102.
[16] Awodele, O.; Oreagba, I. A.; Odoma, S.; da Silva, J. A. & Osunkalu, V. O. (2012): Toxicological evaluation of the aqueous leaf extract of Moringa Oleifera Lam. (Moringaceae). J Ethnopharmacol, 139(2), 330-336.
[17] Adedapo, A. A.; Mogbojuri, O. M.; Emikpe, B. O. (2009): Safety evaluations of the aqueous extract of the leaves of Moringa Oleifera in rats. J Med. Plants Res., 3, 586–591.
[18] Diallo, A.; Eklu-Gadegkeku, K.; Mobio, T.; Moukha, S. & Agbonon, A.; (2009): Protective effect of Moringa Oleifera Lam. and Lannea kerstingii extracts against cadmium and ethanol-induced lipid peroxidation. J Pharmacology and Toxicology, 4, 160–166.
[19] 1Lu, F. C.; Jessup, D. C. and Lavallée, A. (1965): Toxicity of pesticides in young versus adult rats. Food Cosmet. Toxicol., 3, 591–596.
[20] Siddhuraju, P. & Becker, K. (2003): Antioxidant properities of various solvent extracts of total phenolic constituents from three different agroclimatic origins of drumstick tree (M. Oleifera Lam.) Leaves. J agric. food chem., 51, 2144-2155.
[21] Dillard, C. J. & German, J. B. (2000): Phytochemicals: nutraceuticals and human health: A review. J Sci., Food Agric., 80, 1744–1756.
[22] Vladimirov YA, (2004); Reactive oxygen and nitrogen species diagnostic, preventive and therapeutic values. Biochemistry (Moscow), 69: 1-3.
[23] Allen PC, J Lydon and HD Danforth, (1997): Effects of components of Artemisia annua on coccidian infections in chickens. Poult Sci, 76: 1156-1163.
[24] Evans P and B Halliwell, (2001): Micronutrients: oxidant/antioxidantstatus. J Nut, 121: 324-338.
[25] Ovington KS and NC Smith, (1992): Cytokines, free radicals and resistance to Eimeria. Parasitol. Today, 8: 422-426.
[26] Georgieva NV, M Gabrashanska, V Koinarski and Z Yaneva, (2011). Zinc supplementation against Eimeria acervulina-induced oxidative damage in broiler chickens. Vet Med Int, http://dx.doi.org/10.4061/2011/647124
[27] Georgieva NV, M Gabrashanska and V Koinarski, (2011): Ermidou-Pollet S Antioxidant status in Eimeria acervulina infected chickens after dietary selenium treatment. Trace Elem Elec, 28: 42-48.
[28] Ogbe, A. O. & John P. A. (2012): Effect of Polyherbal Aqueous Extracts (Moringa Oleifera , Gum arabic and wild Ganoderma lucidum) in Comparison with Antibiotic on Growth Performance and Haematological Parameters of Broiler Chickens. Res. J. Recent Sci., 1(7), 10–18.
[29] Booth, F. E. M. & Wickens, G. E. (1988): Non-timber uses of selected arid zone trees and shrubs in Africa. FAO conservation guide, Rome, pp 92–101.
[30] Oduro, I.; Ellis, W. O. & Owusu, D. (2008): Nutritional potential of two leafy vegetables: Moringa Oleifera and Impomea batatas leaves. Science Research Essay, 3(2), 57–60.
Author Information
  • Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt

  • Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt

  • Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt

Cite This Article
  • APA Style

    El Banna H. A., M. Atef, Ghazal Nabil. (2016). Anti-coccidial Activity of Moringa Oleifera Plant. Animal and Veterinary Sciences, 4(2), 19-25. https://doi.org/10.11648/j.avs.20160402.12

    Copy | Download

    ACS Style

    El Banna H. A.; M. Atef; Ghazal Nabil. Anti-coccidial Activity of Moringa Oleifera Plant. Anim. Vet. Sci. 2016, 4(2), 19-25. doi: 10.11648/j.avs.20160402.12

    Copy | Download

    AMA Style

    El Banna H. A., M. Atef, Ghazal Nabil. Anti-coccidial Activity of Moringa Oleifera Plant. Anim Vet Sci. 2016;4(2):19-25. doi: 10.11648/j.avs.20160402.12

    Copy | Download

  • @article{10.11648/j.avs.20160402.12,
      author = {El Banna H. A. and M. Atef and Ghazal Nabil},
      title = {Anti-coccidial Activity of Moringa Oleifera Plant},
      journal = {Animal and Veterinary Sciences},
      volume = {4},
      number = {2},
      pages = {19-25},
      doi = {10.11648/j.avs.20160402.12},
      url = {https://doi.org/10.11648/j.avs.20160402.12},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.avs.20160402.12},
      abstract = {Moringa Oleifera leaves were screened for its toxicological effect and some pharmacological activities in broiler chicken. Toxicological effect was studied using upgrading oral doses of 75% ethanolic extract in different groups of mice with monitoring the toxicological symptoms and mortality rate. The anti-coccidal activity of Moringa Oleifera powdered leaves for prevention and its 75% ethanolic extract for treatment of coccidiosis were studied in broilers. The obtained result proved that there were no toxicological symptoms or mortalities obtained till oral administration of 6000 mg/kg b. wt. Inclusion of Moringa olifera powder (0.5 and 1%) to broiler's feed were very efficacious and equal to diclazuril (1ppm) in preventing symptoms of coccidiosis associated with experimental infection with mixed Eimeria species. The anti-coccidal activity of Moringa Oleifera powdered leaves and its 75% ethanolic extract were studied on one-day old chicks. The experimental infected chicks with mixed Eimeria species induced severe coccidiosis in non medicated chicks and led to high mortality reached to (26.76%). Addition of 200 and 400ppm of Moringa Oleifera ethanolic extract induced marked curative effect of cocci diosis manifested by reduced mortality percent and improved PM lesion to be 13.3 and 10% respectively compared to 13.3% for group treated with the reference drug (25ppm) toltrazuril.},
     year = {2016}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - Anti-coccidial Activity of Moringa Oleifera Plant
    AU  - El Banna H. A.
    AU  - M. Atef
    AU  - Ghazal Nabil
    Y1  - 2016/05/04
    PY  - 2016
    N1  - https://doi.org/10.11648/j.avs.20160402.12
    DO  - 10.11648/j.avs.20160402.12
    T2  - Animal and Veterinary Sciences
    JF  - Animal and Veterinary Sciences
    JO  - Animal and Veterinary Sciences
    SP  - 19
    EP  - 25
    PB  - Science Publishing Group
    SN  - 2328-5850
    UR  - https://doi.org/10.11648/j.avs.20160402.12
    AB  - Moringa Oleifera leaves were screened for its toxicological effect and some pharmacological activities in broiler chicken. Toxicological effect was studied using upgrading oral doses of 75% ethanolic extract in different groups of mice with monitoring the toxicological symptoms and mortality rate. The anti-coccidal activity of Moringa Oleifera powdered leaves for prevention and its 75% ethanolic extract for treatment of coccidiosis were studied in broilers. The obtained result proved that there were no toxicological symptoms or mortalities obtained till oral administration of 6000 mg/kg b. wt. Inclusion of Moringa olifera powder (0.5 and 1%) to broiler's feed were very efficacious and equal to diclazuril (1ppm) in preventing symptoms of coccidiosis associated with experimental infection with mixed Eimeria species. The anti-coccidal activity of Moringa Oleifera powdered leaves and its 75% ethanolic extract were studied on one-day old chicks. The experimental infected chicks with mixed Eimeria species induced severe coccidiosis in non medicated chicks and led to high mortality reached to (26.76%). Addition of 200 and 400ppm of Moringa Oleifera ethanolic extract induced marked curative effect of cocci diosis manifested by reduced mortality percent and improved PM lesion to be 13.3 and 10% respectively compared to 13.3% for group treated with the reference drug (25ppm) toltrazuril.
    VL  - 4
    IS  - 2
    ER  - 

    Copy | Download

  • Sections