Molecular Characterization of Indian Potato (Solanum tuberosum L.) Varieties for Cold-Induced Sweetening Using SSR Markers
Journal of Plant Sciences
Volume 3, Issue 4, August 2015, Pages: 191-196
Received: Jun. 27, 2015;
Accepted: Jul. 4, 2015;
Published: Jul. 14, 2015
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Galani Yamdeu Joseph Hubert, Department of Agriculture and Veterinary Medicine, Université des Montagnes, Bangangté, Cameroon
Pooja H. Gupta, Department of Biochemistry, B.A. College of Agriculture, Anand Agricultural University, Gujarat, India
Nilesh J. Patel, Department of Biochemistry, B.A. College of Agriculture, Anand Agricultural University, Gujarat, India
Avadh K. Shah, Department of Biochemistry, B.A. College of Agriculture, Anand Agricultural University, Gujarat, India
Rajeshkumar R. Acharya, Main Vegetable Research Station, Anand Agricultural University, Gujarat, India
Jayantkumar G. Talati, Department of Biochemistry, B.A. College of Agriculture, Anand Agricultural University, Gujarat, India
Cold-induced sweetening developed during storage of potatoes (Solanum tuberosum L.) at low temperature is a crucial factor influencing the processing quality of potato tubers and remains one of the principal concerns of potato processing industry. Developing CIS-resistant genotypes is the most effective method to cope with this stress. In this study, the genetic diversity of 11 Indian potato varieties with different reactions to CIS was assessed using 10 SSR primers. The primers detected a total of 42 alleles arranged in 44 different configurations, among which 37 alleles (88%) were polymorphic. The polymorphic information content (PIC) value of the SSR locus ranged from 0.473 to 0.787 thus indicating a high utility of these markers for study of genetic diversity in potato. A number of polymorphic fragments appeared to be specific to a given sugar-forming group. Primer Sti007 generated one fragment Sti007131bp present only in all the high sugar-forming varieties. The dendrogram derived from Dice’s similarity coefficients among the 11 varieties could partially but efficiently differentiate close parents and sugar-forming groups among the varieties. These findings demonstrate the effectiveness of SSR markers to assess the genetic variation among potato cultivars in order to develop molecular markers associated with CIS to improve potato breeding programs.
Galani Yamdeu Joseph Hubert,
Pooja H. Gupta,
Nilesh J. Patel,
Avadh K. Shah,
Rajeshkumar R. Acharya,
Jayantkumar G. Talati,
Molecular Characterization of Indian Potato (Solanum tuberosum L.) Varieties for Cold-Induced Sweetening Using SSR Markers, Journal of Plant Sciences.
Vol. 3, No. 4,
2015, pp. 191-196.
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