Purification and Toxicity Study of a Saponin from Seeds of <i>Albizia odorata</i>, a Fabaceae from Madagascar

Clara Fredeline Rajemiarimoelisoa; Danielle Aurore Doll Rakoto; Hanitra Ranjana Randrianarivo; Victor Louis Jeannoda

doi:doi:10.11648/j.jps.20150305.14

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Purification and Toxicity Study of a Saponin from Seeds of Albizia odorata, a Fabaceae from Madagascar

Clara Fredeline Rajemiarimoelisoa, Danielle Aurore Doll Rakoto, Hanitra Ranjana Randrianarivo, Victor Louis Jeannoda

Published in Journal of Plant Sciences (Volume 3, Issue 5)

Received: 19 September 2015 Accepted: 7 October 2015 Published: 19 October 2015

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Abstract

In order to continue the research of natural compounds of interest such as pesticides and therapeutic molecules in endemic species of Albizia from Madagascar, potentials of Albizia odorata seed extract were assessed. A toxic saponin (saponoside), named Albodorine, was isolated by extraction with hot ethanol or distilled water followed by purification procedure comprising n-butanol partition, precipitation by aceton-diethyl ether (50/50), Sephadex LH-20 gel chromatography and silica gel chromatography. All these methods were guided by toxicity tests on mice and homogeneity tests by Thin Layer Chromatography (TLC). Albodorine was thermostable, soluble in water and organic solvents and tasted bitter. Its acidic hydrolysis released glucose, arabinose and rhamnose. Tested on different experimental animal models, it was toxic to warm and cold blooded animals. In mouse, when intraperitoneally administered, it caused acute intoxication mainly presented as hyperpnea, ataxia and terminal seizures before the animal died. Its LD50 was about 9 mg/kg of mouse body weight by intraperitoneal route. In different organs, it caused histopathological lesions characterized by vascular congestions and important hemorrhage in liver, lungs and kidneys. In vitro, it reduced the heart rate and force of contraction of isolated rat atria. It had hemolytic activity. Albodorine showed toxicological properties that could be exploited under certain conditions for the control of harmful organisms.

Published in	Journal of Plant Sciences (Volume 3, Issue 5)
DOI	10.11648/j.jps.20150305.14
Page(s)	264-271
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Albizia odorata, Toxin, Albodorine, Saponin, Hemolysis, Histopathology, Isolated Atria, Inotropic Effect

References

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Clara Fredeline Rajemiarimoelisoa, Danielle Aurore Doll Rakoto, Hanitra Ranjana Randrianarivo, Victor Louis Jeannoda. (2015). Purification and Toxicity Study of a Saponin from Seeds of Albizia odorata, a Fabaceae from Madagascar. Journal of Plant Sciences, 3(5), 264-271. https://doi.org/10.11648/j.jps.20150305.14

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ACS Style

Clara Fredeline Rajemiarimoelisoa; Danielle Aurore Doll Rakoto; Hanitra Ranjana Randrianarivo; Victor Louis Jeannoda. Purification and Toxicity Study of a Saponin from Seeds of Albizia odorata, a Fabaceae from Madagascar. J. Plant Sci. 2015, 3(5), 264-271. doi: 10.11648/j.jps.20150305.14

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AMA Style

Clara Fredeline Rajemiarimoelisoa, Danielle Aurore Doll Rakoto, Hanitra Ranjana Randrianarivo, Victor Louis Jeannoda. Purification and Toxicity Study of a Saponin from Seeds of Albizia odorata, a Fabaceae from Madagascar. J Plant Sci. 2015;3(5):264-271. doi: 10.11648/j.jps.20150305.14

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@article{10.11648/j.jps.20150305.14,
  author = {Clara Fredeline Rajemiarimoelisoa and Danielle Aurore Doll Rakoto and Hanitra Ranjana Randrianarivo and Victor Louis Jeannoda},
  title = {Purification and Toxicity Study of a Saponin from Seeds of Albizia odorata, a Fabaceae from Madagascar},
  journal = {Journal of Plant Sciences},
  volume = {3},
  number = {5},
  pages = {264-271},
  doi = {10.11648/j.jps.20150305.14},
  url = {https://doi.org/10.11648/j.jps.20150305.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20150305.14},
  abstract = {In order to continue the research of natural compounds of interest such as pesticides and therapeutic molecules in endemic species of Albizia from Madagascar, potentials of Albizia odorata seed extract were assessed. A toxic saponin (saponoside), named Albodorine, was isolated by extraction with hot ethanol or distilled water followed by purification procedure comprising n-butanol partition, precipitation by aceton-diethyl ether (50/50), Sephadex LH-20 gel chromatography and silica gel chromatography. All these methods were guided by toxicity tests on mice and homogeneity tests by Thin Layer Chromatography (TLC). Albodorine was thermostable, soluble in water and organic solvents and tasted bitter. Its acidic hydrolysis released glucose, arabinose and rhamnose. Tested on different experimental animal models, it was toxic to warm and cold blooded animals. In mouse, when intraperitoneally administered, it caused acute intoxication mainly presented as hyperpnea, ataxia and terminal seizures before the animal died. Its LD50 was about 9 mg/kg of mouse body weight by intraperitoneal route. In different organs, it caused histopathological lesions characterized by vascular congestions and important hemorrhage in liver, lungs and kidneys. In vitro, it reduced the heart rate and force of contraction of isolated rat atria. It had hemolytic activity. Albodorine showed toxicological properties that could be exploited under certain conditions for the control of harmful organisms.},
 year = {2015}
}

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TY - JOUR
T1 - Purification and Toxicity Study of a Saponin from Seeds of Albizia odorata, a Fabaceae from Madagascar
AU - Clara Fredeline Rajemiarimoelisoa
AU - Danielle Aurore Doll Rakoto
AU - Hanitra Ranjana Randrianarivo
AU - Victor Louis Jeannoda
Y1 - 2015/10/19
PY - 2015
N1 - https://doi.org/10.11648/j.jps.20150305.14
DO - 10.11648/j.jps.20150305.14
T2 - Journal of Plant Sciences
JF - Journal of Plant Sciences
JO - Journal of Plant Sciences
SP - 264
EP - 271
PB - Science Publishing Group
SN - 2331-0731
UR - https://doi.org/10.11648/j.jps.20150305.14
AB - In order to continue the research of natural compounds of interest such as pesticides and therapeutic molecules in endemic species of Albizia from Madagascar, potentials of Albizia odorata seed extract were assessed. A toxic saponin (saponoside), named Albodorine, was isolated by extraction with hot ethanol or distilled water followed by purification procedure comprising n-butanol partition, precipitation by aceton-diethyl ether (50/50), Sephadex LH-20 gel chromatography and silica gel chromatography. All these methods were guided by toxicity tests on mice and homogeneity tests by Thin Layer Chromatography (TLC). Albodorine was thermostable, soluble in water and organic solvents and tasted bitter. Its acidic hydrolysis released glucose, arabinose and rhamnose. Tested on different experimental animal models, it was toxic to warm and cold blooded animals. In mouse, when intraperitoneally administered, it caused acute intoxication mainly presented as hyperpnea, ataxia and terminal seizures before the animal died. Its LD50 was about 9 mg/kg of mouse body weight by intraperitoneal route. In different organs, it caused histopathological lesions characterized by vascular congestions and important hemorrhage in liver, lungs and kidneys. In vitro, it reduced the heart rate and force of contraction of isolated rat atria. It had hemolytic activity. Albodorine showed toxicological properties that could be exploited under certain conditions for the control of harmful organisms.
VL - 3
IS - 5
ER -

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Author Information

Clara Fredeline Rajemiarimoelisoa

Department of Pharmacy, Faculty of Medicine, Antananarivo, University of Antananarivo, Madagascar
Danielle Aurore Doll Rakoto

Laboratory of Applied Biochemistry to Medical Sciences, Fundamental and Applied Biochemistry Department, Faculty of Sciences, University of Antananarivo, Antananarivo, Madagascar
Hanitra Ranjana Randrianarivo

Laboratory of Applied Biochemistry to Medical Sciences, Fundamental and Applied Biochemistry Department, Faculty of Sciences, University of Antananarivo, Antananarivo, Madagascar
Victor Louis Jeannoda

Laboratory of Applied Biochemistry to Medical Sciences, Fundamental and Applied Biochemistry Department, Faculty of Sciences, University of Antananarivo, Antananarivo, Madagascar

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Clara Fredeline Rajemiarimoelisoa, Danielle Aurore Doll Rakoto, Hanitra Ranjana Randrianarivo, Victor Louis Jeannoda. (2015). Purification and Toxicity Study of a Saponin from Seeds of Albizia odorata, a Fabaceae from Madagascar. Journal of Plant Sciences, 3(5), 264-271. https://doi.org/10.11648/j.jps.20150305.14

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Clara Fredeline Rajemiarimoelisoa; Danielle Aurore Doll Rakoto; Hanitra Ranjana Randrianarivo; Victor Louis Jeannoda. Purification and Toxicity Study of a Saponin from Seeds of Albizia odorata, a Fabaceae from Madagascar. J. Plant Sci. 2015, 3(5), 264-271. doi: 10.11648/j.jps.20150305.14

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AMA Style

Clara Fredeline Rajemiarimoelisoa, Danielle Aurore Doll Rakoto, Hanitra Ranjana Randrianarivo, Victor Louis Jeannoda. Purification and Toxicity Study of a Saponin from Seeds of Albizia odorata, a Fabaceae from Madagascar. J Plant Sci. 2015;3(5):264-271. doi: 10.11648/j.jps.20150305.14

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@article{10.11648/j.jps.20150305.14,
  author = {Clara Fredeline Rajemiarimoelisoa and Danielle Aurore Doll Rakoto and Hanitra Ranjana Randrianarivo and Victor Louis Jeannoda},
  title = {Purification and Toxicity Study of a Saponin from Seeds of Albizia odorata, a Fabaceae from Madagascar},
  journal = {Journal of Plant Sciences},
  volume = {3},
  number = {5},
  pages = {264-271},
  doi = {10.11648/j.jps.20150305.14},
  url = {https://doi.org/10.11648/j.jps.20150305.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20150305.14},
  abstract = {In order to continue the research of natural compounds of interest such as pesticides and therapeutic molecules in endemic species of Albizia from Madagascar, potentials of Albizia odorata seed extract were assessed. A toxic saponin (saponoside), named Albodorine, was isolated by extraction with hot ethanol or distilled water followed by purification procedure comprising n-butanol partition, precipitation by aceton-diethyl ether (50/50), Sephadex LH-20 gel chromatography and silica gel chromatography. All these methods were guided by toxicity tests on mice and homogeneity tests by Thin Layer Chromatography (TLC). Albodorine was thermostable, soluble in water and organic solvents and tasted bitter. Its acidic hydrolysis released glucose, arabinose and rhamnose. Tested on different experimental animal models, it was toxic to warm and cold blooded animals. In mouse, when intraperitoneally administered, it caused acute intoxication mainly presented as hyperpnea, ataxia and terminal seizures before the animal died. Its LD50 was about 9 mg/kg of mouse body weight by intraperitoneal route. In different organs, it caused histopathological lesions characterized by vascular congestions and important hemorrhage in liver, lungs and kidneys. In vitro, it reduced the heart rate and force of contraction of isolated rat atria. It had hemolytic activity. Albodorine showed toxicological properties that could be exploited under certain conditions for the control of harmful organisms.},
 year = {2015}
}

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