Characterization of chemical groups, and investigation of cytotoxic and antioxidant activity of Litsea glutinosa leaves
Journal of Plant Sciences
Volume 2, Issue 6-1, December 2014, Pages: 24-29
Received: Nov. 14, 2014;
Accepted: Nov. 19, 2014;
Published: Jun. 23, 2015
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Rumpa Bhowmick, Department of Pharmacy, Noakhali Science and Technology University, Noakhali, Bangladesh
Md. Shahid Sarwar, Department of Pharmacy, Southeast University, Dhaka, Bangladesh
Syed Masudur Rahman Dewan, Department of Pharmacy, Noakhali Science and Technology University, Noakhali, Bangladesh
Abhijit Das, Department of Pharmacy, Noakhali Science and Technology University, Noakhali, Bangladesh
Binayok Das, Department of Oncology, Ashic Oncology and Palliative Care Center, Dhaka, Bangladesh
Md. Mohiuddin, Department of Pharmacy, Southeast University, Dhaka, Bangladesh
K. M. Rahat Maruf Jitu, Department of Pharmacy, Noakhali Science and Technology University, Noakhali, Bangladesh
Mohammad Safiqul Islam, Department of Pharmacy, Noakhali Science and Technology University, Noakhali, Bangladesh
The present study was conducted to investigate possible phytochemicals, cytotoxic activity, total phenolic content and antioxidant property of different extracts of Litsea glutinosa leaves. To determine different pytochemicals, various standard group tests were done. For cytotoxicity test, brine shrimp lethality bioassay was carried out. To evaluate the antioxidant properties, some complementary test systems, namely DPPH free-radical scavenging assay and reducing power assay and determination of total phenolic content were conducted. The phytochemical analysis revealed the presence of alkaloids, carbohydrate, flavonoid, saponin, gum, steroid, tannins and terpinoids. In brine shrimp lethality bioassay, results showed that all the extracts possess significant (P˂0.05) activity when compared to the standard, Colchicine. Among the extracts, the n-hexane soluble fraction showed the highest activity (LC50 30.32±0.46µg/ml) which is very close to the standard (LC50 30.11±0.30µg/ml) used. In DPPH free radical scavenging test, IC50 value of the ethyl acetate extract was found fairly significant (9.68±0.15μg/ml) while compared to that of the reference standards ascorbic acid (1.82±0.15μg/ml). In reducing power assay, the maximum reducing capacity for the methanolic extract was found 257.67±4.04 at 200μg/ml while compared to standard ascorbic acid (356.33±5.68μg/ml). The total phenolic amount was also calculated as quite high in the ethyl acetate extract (69.00±0.58mg/g of gallic acid equivalent). Presence of significant antioxidant properties of different extracts would justify its traditional use. However, it would be interesting to investigate possible causes and their mechanisms responsible for the cytotoxic and antioxidant properties of the plant L. glutinosa.
Md. Shahid Sarwar,
Syed Masudur Rahman Dewan,
K. M. Rahat Maruf Jitu,
Mohammad Safiqul Islam,
Characterization of chemical groups, and investigation of cytotoxic and antioxidant activity of Litsea glutinosa leaves, Journal of Plant Sciences. Special Issue: Pharmacological and Biological Investigation of Medicinal Plants.
Vol. 2, No. 6-1,
2014, pp. 24-29.
A. Ghani, “Medicinal plants of Bangladesh: Chemical constituents and uses”, Nimtali, Dhaka: Asiatic Society of Bangladesh, 2003, pp. 285.
K. Kirtikar, and B. Basu, “Indian Medicinal Plants (revised issue)”, vol.3, Allahabad, 1981, pp. 2156-2161.
U. Chomkamon, S. Nongluksna, De-E. Wanchai, and R. Nijsiri, “Chemical composition and antimicrobial activity of essential oils from plants in Litsea spp.”, Technical information services (TIS)/MUTT, 2000, pp. 18-20,
A. Chatterjee, and S. Pakrashi, “The Treatise of Indian Medicinal Plants”, vol. I, New Delhi, India: INSA, 1994, pp. 107.
J. Loliger, “Natural antioxidants”, Lipid. Technol., vol. 3, pp. 58-61, 1991.
C. Van Wagenen, R. Larsen, J. H. Cardellina, D. Ran dazzo, Z. C. Lidert, and C. Swithenbank, “Ulosantoin, a potent insecticide from the sponge Ulosa ruetzleri”, J. Org. Chem., vol. 58, pp. 335-337, 1993.
S. M. R. Dewan, and A. Das, “Investigation of in vitro thrombolytic potential and phytochemical nature of Crinum latifolium L. leaves growing in coastal region of Bangladesh”, Int. J. Bio. Pharm. Res., vol. 4, pp. 1-7, 2013.
T.S. Roopashree, R. Dang, S. R. H. Rani, and C. Narendra, “Antibacterial activity of anti-psoriatic herbs: Cassiatora, Momordica charantia and Calendula officinalis”, Int. J. App. Res. Nat. Prod., vol. 1, pp. 20-28, 2008.
N. Meyer, N. R. Ferrighi, J. E. Putnam, L. B. Jacobsen, D. E. Nichols, J. L. McLaughlin, “Brine shrimp: A convenient general bioassay for active plant constituents”, Planta. Medica., vol. 45, pp. 31-34, 1982.
S. T. Chang, J. H. Wu, S. Y. Wang, P. L. Kang, N. S. Yang, and L. F. Shyur, “Antioxidant activity of extracts from Acacia confusa bark and heartwood”, J. Agric. Food. Chem., vol. 49, pp. 3420–3424, 2001.
A. Dehpour, M. A. Ebrahimzadeh, S. F. Nabavi, and S. M. Nabavi, “Antioxidant activity of methanol extract of Ferula assafoetida and its essential oil composition”, Grasas. Aceites., vol. 60, pp. 405-412, 2009.
K. Wolfe, X. Wu, and R. H. Liu, “Antioxidant activity of apple peels”, J. Agric. Food. Chem., vol. 51, pp. 609-614, 2003.
M. O. Ullah, M. Haque, K. F. Urmi, A. H. M. Zulfiker, E. S. Anita, M. Begum, and K. Hamid, “Anti-bacterial activity and brine shrimp lethality bioassay of methanolic extracts of fourteen different edible vegetables from Bangladesh”, Asian. Pac. J. Trop. Biomed., vol. 3, pp. 1-7, 2013.
P. H. Parikh, and A. Y. Rangrez, “Extraction and Phytochemical Evaluation of Litsea Glutinosa Bark Methanolic Extract”, J. App. Pharm. Sci., vol. 2, pp. 71-78, 2012.
N. Benariba, R. Djaziri, W. Bellakhdar, N. Belkacem, M. Kadiata, W. J. Malaisse, and A. Sener, “Phytochemical screening and free radical scavenging activity of Citrullus colocynthis seeds extracts”, Asian. Pac. J. Trop. Biomed., vol. 3, pp. 35-40, 2013.
P. Tiwari, B. Kumar, M. Kaur, G. Kaur, and H. Kaur, “Phytochemical screening and extraction”, Int. Pharm. Sciencia., vol. 1, pp. 98-106, 2011.
M. Firdaus, A. A. Prihanto, and R. Nurdiani, “Antioxidant and cytotoxic activity of Acanthus ilicifolius flower”, Asian. Pac. J. Trop. Biomed., vol. 3, pp. 17-21, 2013.
S. Man, W. Gao, Y. Zhang, L. Huang, and C. Liu, “Chemical study and medical application of saponins as anti-cancer agents”, Fitoterapia., vol. 81, pp. 703-714, 2010.
M. Imai, H. Kikuchi, T. Denda, K. Ohyama, C. Hirobe, and H. Toyoda, “Cytotoxic effects of flavonoids against a human colon cancer derived cell line, COLO 201: A potential natural anti-cancer substance”, Cancer. Letters., vol. 276, pp. 74-80, 2009.
A. Gonzalez-Sarrias, L. Li, and N. P. Seeram, “Anticancer effects of maple syrup phenolics and extracts on proliferation, apoptosis, and cell cycle arrest of human colon cells”, J. Functional. Foods., vol. 4, pp. 185-196, 2012.
S. Muruhan, S. Selvaraj, and P. K. Viswanathan, “In vitro antioxidant activities of Solanum surattense leaf extract”, Asian. Pac. J. Trop. Biomed., vol. 3, pp. 28-34, 2013.
M. Elmastas, O. Isildak, I. Turkekul, and N. Temur, “Determination of antioxidant activity and antioxidant compounds in wild edible mushrooms”, J. Food. Comp. Anal., vol. 20, pp. 337-345, 2007.
A. Rezaeizadeh, Z. B. A. B. Zakaria, M. Abdollahi, G. Y. Meng, N. M. Mustapha, M. B. Hamid, and T. A. B.T. Ibrahim, “Antioxidant and antihyperglycaemic effects of an aqueous extract from Momordica charantia fruit in a type II diabetic rat model”, J. Med. Plant. Res., vol. 5, pp. 2990-2901, 2011.
M. Wani, A. Sharma, J. Deshpande, S. Mathew, and M. B. Khetmalas, “Qualitative phytochemical screening of three indigenous medicinal plants”, Res. J. Pharm. Biol. Chem. Sci., vol. 4, pp. 287-291, 2013.
L. Tomsone, Z. Kruma, and R. Galoburda, “Comparison of different solvents and extraction methods for isolation of phenolic compounds from Horseradish roots (Armoracia rusticana)”, World. Academy. Sci. Eng. Technol., vol. 6, pp. 903-908, 2012.
X. Duan, G. Wu, and Y. Jiang, “Evaluation of antioxidant properties of phenolics from litchi fruit in relation to pericarp browning prevention”, Molecules., vol. 12, pp. 759-771, 2007.
M. Ajila, K. A. Naidu, S. G. Bhat, and U. J. S. P. Rao, “Bioactive compounds of mango peel extract”, Food. Chem., vol. 105, pp. 982-988, 2007.
A. Roy, K. Khanra, A. Mishra, and N. Bhattacharyya, “General analysis and antioxidant study of traditional fermented drink Handia, its concentrate and volatiles”, Adv. Life. Sci. Appl., vol. 1, pp. 54–57, 2012.
Y. C. Chung, C. T. Chang, W. W. Chao, C. F. Lin, and S. T. Chou, “Antioxidative activity and safety of the 50% ethanolic extract from red bean fermented by Bacillus subtilis IMR-NK1”, J. Agric. Food. Chem., vol. 50, pp. 2454–2458, 2002.
S. R. Valvi, V. S. Rathod, D. P. Yesane, “Screening of three wild edible fruits for their antioxidant potential”, Curr. Bot., vol. 2, pp. 48-52, 2011.
M. Naczk, and F. Shahidi, “Phenolic in cereals, fruit and vegetables: Occurrence, extraction and analysis”, J. Pharm. Biomed. Anal., vol. 41, pp. 1523-1542, 2006.
N. Nićiforović, V. Mihailović, P. Mašković, S. Solujić, A. Stojković, and D. P. Muratspahić, “Antioxidant activity of selected plant species; potential new sources of natural antioxidants”, Food. Chem. Toxicol., vol. 48, pp. 3125-3130, 2010.
R. Horax, N. Hettiarachchy, and S. Islam, “Total Phenolic contents and phenolic acid constituents in 4 varieties of bitter melons (Momordica charantia) and antioxidant activities of their extracts”, J. Food. Sci., vol. 70, pp. 275-280, 2005.