Journal of Plant Sciences

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Characterization of chemical groups, and investigation of cytotoxic and antioxidant activity of Litsea glutinosa leaves

Received: 14 November 2014    Accepted: 19 November 2014    Published: 23 June 2015
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Abstract

The present study was conducted to investigate possible phytochemicals, cytotoxic activity, total phenolic content and antioxidant property of different extracts of Litsea glutinosa leaves. To determine different pytochemicals, various standard group tests were done. For cytotoxicity test, brine shrimp lethality bioassay was carried out. To evaluate the antioxidant properties, some complementary test systems, namely DPPH free-radical scavenging assay and reducing power assay and determination of total phenolic content were conducted. The phytochemical analysis revealed the presence of alkaloids, carbohydrate, flavonoid, saponin, gum, steroid, tannins and terpinoids. In brine shrimp lethality bioassay, results showed that all the extracts possess significant (P˂0.05) activity when compared to the standard, Colchicine. Among the extracts, the n-hexane soluble fraction showed the highest activity (LC50 30.32±0.46µg/ml) which is very close to the standard (LC50 30.11±0.30µg/ml) used. In DPPH free radical scavenging test, IC50 value of the ethyl acetate extract was found fairly significant (9.68±0.15μg/ml) while compared to that of the reference standards ascorbic acid (1.82±0.15μg/ml). In reducing power assay, the maximum reducing capacity for the methanolic extract was found 257.67±4.04 at 200μg/ml while compared to standard ascorbic acid (356.33±5.68μg/ml). The total phenolic amount was also calculated as quite high in the ethyl acetate extract (69.00±0.58mg/g of gallic acid equivalent). Presence of significant antioxidant properties of different extracts would justify its traditional use. However, it would be interesting to investigate possible causes and their mechanisms responsible for the cytotoxic and antioxidant properties of the plant L. glutinosa.

DOI 10.11648/j.jps.s.2014020601.15
Published in Journal of Plant Sciences (Volume 2, Issue 6-1, December 2014)

This article belongs to the Special Issue Pharmacological and Biological Investigation of Medicinal Plants

Page(s) 24-29
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Antioxidant Activities, Cytotoxicity, DPPH Free Radical Scavenging, Litsea Glutinosa, Total Phenolic Content

References
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Author Information
  • Department of Pharmacy, Noakhali Science and Technology University, Noakhali, Bangladesh

  • Department of Pharmacy, Southeast University, Dhaka, Bangladesh

  • Department of Pharmacy, Noakhali Science and Technology University, Noakhali, Bangladesh

  • Department of Pharmacy, Noakhali Science and Technology University, Noakhali, Bangladesh

  • Department of Oncology, Ashic Oncology and Palliative Care Center, Dhaka, Bangladesh

  • Department of Pharmacy, Southeast University, Dhaka, Bangladesh

  • Department of Pharmacy, Noakhali Science and Technology University, Noakhali, Bangladesh

  • Department of Pharmacy, Noakhali Science and Technology University, Noakhali, Bangladesh

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  • APA Style

    Rumpa Bhowmick, Md. Shahid Sarwar, Syed Masudur Rahman Dewan, Abhijit Das, Binayok Das, et al. (2015). Characterization of chemical groups, and investigation of cytotoxic and antioxidant activity of Litsea glutinosa leaves. Journal of Plant Sciences, 2(6-1), 24-29. https://doi.org/10.11648/j.jps.s.2014020601.15

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    ACS Style

    Rumpa Bhowmick; Md. Shahid Sarwar; Syed Masudur Rahman Dewan; Abhijit Das; Binayok Das, et al. Characterization of chemical groups, and investigation of cytotoxic and antioxidant activity of Litsea glutinosa leaves. J. Plant Sci. 2015, 2(6-1), 24-29. doi: 10.11648/j.jps.s.2014020601.15

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    AMA Style

    Rumpa Bhowmick, Md. Shahid Sarwar, Syed Masudur Rahman Dewan, Abhijit Das, Binayok Das, et al. Characterization of chemical groups, and investigation of cytotoxic and antioxidant activity of Litsea glutinosa leaves. J Plant Sci. 2015;2(6-1):24-29. doi: 10.11648/j.jps.s.2014020601.15

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  • @article{10.11648/j.jps.s.2014020601.15,
      author = {Rumpa Bhowmick and Md. Shahid Sarwar and Syed Masudur Rahman Dewan and Abhijit Das and Binayok Das and Md. Mohiuddin and K. M. Rahat Maruf Jitu and Mohammad Safiqul Islam},
      title = {Characterization of chemical groups, and investigation of cytotoxic and antioxidant activity of Litsea glutinosa leaves},
      journal = {Journal of Plant Sciences},
      volume = {2},
      number = {6-1},
      pages = {24-29},
      doi = {10.11648/j.jps.s.2014020601.15},
      url = {https://doi.org/10.11648/j.jps.s.2014020601.15},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.jps.s.2014020601.15},
      abstract = {The present study was conducted to investigate possible phytochemicals, cytotoxic activity, total phenolic content and antioxidant property of different extracts of Litsea glutinosa leaves. To determine different pytochemicals, various standard group tests were done. For cytotoxicity test, brine shrimp lethality bioassay was carried out. To evaluate the antioxidant properties, some complementary test systems, namely DPPH free-radical scavenging assay and reducing power assay and determination of total phenolic content were conducted. The phytochemical analysis revealed the presence of alkaloids, carbohydrate, flavonoid, saponin, gum, steroid, tannins and terpinoids. In brine shrimp lethality bioassay, results showed that all the extracts possess significant (P˂0.05) activity when compared to the standard, Colchicine. Among the extracts, the n-hexane soluble fraction showed the highest activity (LC50 30.32±0.46µg/ml) which is very close to the standard (LC50 30.11±0.30µg/ml) used. In DPPH free radical scavenging test, IC50 value of the ethyl acetate extract was found fairly significant (9.68±0.15μg/ml) while compared to that of the reference standards ascorbic acid (1.82±0.15μg/ml). In reducing power assay, the maximum reducing capacity for the methanolic extract was found 257.67±4.04 at 200μg/ml while compared to standard ascorbic acid (356.33±5.68μg/ml). The total phenolic amount was also calculated as quite high in the ethyl acetate extract (69.00±0.58mg/g of gallic acid equivalent). Presence of significant antioxidant properties of different extracts would justify its traditional use. However, it would be interesting to investigate possible causes and their mechanisms responsible for the cytotoxic and antioxidant properties of the plant L. glutinosa.},
     year = {2015}
    }
    

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  • TY  - JOUR
    T1  - Characterization of chemical groups, and investigation of cytotoxic and antioxidant activity of Litsea glutinosa leaves
    AU  - Rumpa Bhowmick
    AU  - Md. Shahid Sarwar
    AU  - Syed Masudur Rahman Dewan
    AU  - Abhijit Das
    AU  - Binayok Das
    AU  - Md. Mohiuddin
    AU  - K. M. Rahat Maruf Jitu
    AU  - Mohammad Safiqul Islam
    Y1  - 2015/06/23
    PY  - 2015
    N1  - https://doi.org/10.11648/j.jps.s.2014020601.15
    DO  - 10.11648/j.jps.s.2014020601.15
    T2  - Journal of Plant Sciences
    JF  - Journal of Plant Sciences
    JO  - Journal of Plant Sciences
    SP  - 24
    EP  - 29
    PB  - Science Publishing Group
    SN  - 2331-0731
    UR  - https://doi.org/10.11648/j.jps.s.2014020601.15
    AB  - The present study was conducted to investigate possible phytochemicals, cytotoxic activity, total phenolic content and antioxidant property of different extracts of Litsea glutinosa leaves. To determine different pytochemicals, various standard group tests were done. For cytotoxicity test, brine shrimp lethality bioassay was carried out. To evaluate the antioxidant properties, some complementary test systems, namely DPPH free-radical scavenging assay and reducing power assay and determination of total phenolic content were conducted. The phytochemical analysis revealed the presence of alkaloids, carbohydrate, flavonoid, saponin, gum, steroid, tannins and terpinoids. In brine shrimp lethality bioassay, results showed that all the extracts possess significant (P˂0.05) activity when compared to the standard, Colchicine. Among the extracts, the n-hexane soluble fraction showed the highest activity (LC50 30.32±0.46µg/ml) which is very close to the standard (LC50 30.11±0.30µg/ml) used. In DPPH free radical scavenging test, IC50 value of the ethyl acetate extract was found fairly significant (9.68±0.15μg/ml) while compared to that of the reference standards ascorbic acid (1.82±0.15μg/ml). In reducing power assay, the maximum reducing capacity for the methanolic extract was found 257.67±4.04 at 200μg/ml while compared to standard ascorbic acid (356.33±5.68μg/ml). The total phenolic amount was also calculated as quite high in the ethyl acetate extract (69.00±0.58mg/g of gallic acid equivalent). Presence of significant antioxidant properties of different extracts would justify its traditional use. However, it would be interesting to investigate possible causes and their mechanisms responsible for the cytotoxic and antioxidant properties of the plant L. glutinosa.
    VL  - 2
    IS  - 6-1
    ER  - 

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