American Journal of Bioscience and Bioengineering
Volume 3, Issue 5, October 2015, Pages: 40-46
Received: Aug. 31, 2015;
Accepted: Sep. 13, 2015;
Published: Sep. 26, 2015
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Majid Hussein Al-Jailawi, Department of Molecular & Medical Biotechnology, College of Biotechnology, Al-Nahrain University, Baghdad, Iraq
Albab Fawaz Al-Faraas, Department of Biotechnology, College of Science, Al-Nahrain University, Baghdad, Iraq
Abdelghani Ibrahem Yahia, Department of Molecular & Medical Biotechnology, College of Biotechnology, Al-Nahrain University, Baghdad, Iraq
This study aimed to obtain efficient bacteria capable of desulfurizing dibenzothiophene (DBT). For this purpose forty oil contaminated soil samples were collected from different sites in Iraq. It was found that three isolates (M9, M19 and S25) had the ability to desulfurize DBT (cleave C-S bond) and converted it to 2-hydroxybiphenel (2-HBP) or other phenolic end products. This suggests the involvement of the 4S pathway in the desulfurizing of DBT via a specific cleavage of only the C-S bond by these isolates. These isolates were identified as Pseudomonas aeruginosa. The result also showed that P. aeruginosa S25 was the most efficient one for removing sulfur from DBT. The GC/MS analysis for DBT after growth of P. aeruginosa S25, indicated that 12.89 % of DBT was consumed (consumption of sulfur), and the product (2-HBP) was further converted to 2-MBP (addition of a methyl group) and this could be a novel pathway for consuming DBT.
Majid Hussein Al-Jailawi,
Albab Fawaz Al-Faraas,
Abdelghani Ibrahem Yahia,
Isolation and Identification of Dibenzothiophene Biodesulfurizing Bacteria, American Journal of Bioscience and Bioengineering.
Vol. 3, No. 5,
2015, pp. 40-46.
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