American Journal of Bioscience and Bioengineering

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Histological Changes in Calcium Regulating Endocrine Gland (Ultimobranchial) After Dimethoate Exposure in Freshwater Catfish Heteropneustes Fossilis

Received: 14 September 2015    Accepted: 30 September 2015    Published: 28 October 2015
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Abstract

Ultimobranchial gland in fishes is associated primarily with calcium regulation. The study was aimed to find effect of an organophosphate pesticide dimethoate on serum Ca2+ and ultimobranchial gland in freshwater catfish Heteropneustes fossilis. Fish were exposed to dimethoate at 2.24 mg/l (75% of 96 h LC50) for short-term (24, 48, 72 and 96 h), and 25% of 96 h LC50 (1.00 mg/l) for long-term (6, 12, 24 and 36 d). The blood samples collected at assigned durations from the anaesthetized fish were used to measure serum Ca2+ levels. At the same time UBG were also extricated from the fish by removing the tissues adjoining esophagus, liver and sinus venosus of the heart for subsequent histological examination. A significant (P<0.05) increase in serum Ca2+ levels of fish following 24 h and 6d dimethoate exposure was observed. However, after 48 h and 12 d the Ca2+ level gradually begins to decline exhibiting significant (P<0.0001) hypocalcaemia after 96 h and 36 d in the exposed fishes. The histological examination of exposed fish glands exhibit significant changes in their cellular activity, staining property, nuclear diameter and nuclear volume, vacuolization and degeneration. It is concluded that dimethoate severely hampers the calcium homeostasis in Heteropneustes fossilis; therefore the pesticide should be cautiously used near aquatic bodies.

DOI 10.11648/j.bio.20150305.22
Published in American Journal of Bioscience and Bioengineering (Volume 3, Issue 5, October 2015)
Page(s) 91-98
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Ultimobranchial Gland, Dimethoate, Calcium, Heteropneustes fossilis

References
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[2] Pandey RK, Singh RN, Singh S, Singh NN and Das VK: Acute toxicity bioassay of dimethoate on freshwater air breathing catfish, Heteropneustes fossilis (Bloch) and its behavioural studies. J. Environ. Biol. 30 (3): 437-440, 2009 b.
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[5] Pandey RK and Das VK: Dimethoate induced changes in serum Ca2+ and Corpuscles of Stannius in freshwater catfish Heteropneustes fossilis, after short-term and long-term exposure. Am. J. Life Sci. 3(1): 17-21, 2015.
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[17] Yildirim MZ, Benli AC, Selvi M, Ozkul A, Erkoc F and Kocak O: Acute toxicity, behavioral changes, and histopathological effects of deltamethrin on tissues (gills, liver, brain, spleen, kidney, muscle, skin) of Nile tilapia (Oreochromis nilkoticus L.) fingerlings. Environ. Toxicol. 21: 614-620, 2006.
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[19] Peebua P, Kruatrachue M, Pokethitiyook P and Singhakaew S: Histopathological alterations of Nile tilapia, Oreochromis niloticus in acute and subchronic alachlor exposure. J. Environ. Biol. 29 (3): 325-331, 2008.
[20] Parikh PH, Rangrez A, Bagchi RA and Desai BN: Effect of dimethoate on some histoarchitecture of freshwater fish Oreocromis mossambicus (Peters, 1852). Bioscan. 5: 55-58, 2010.
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[28] Prasad MR, Kumar A, Mishra D, Srivastav SK and Srivastav AK: Blood electrolytes of the freshwater catfish Heteropneustes fossilis in response to treatment with a botanical pesticide (latex of Euphorbia royleana). Integrat. Zool. 6 (2): 150-156, 2011.
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Author Information
  • Department of Zoology, Kamla Nehru Institute of Physical and Social Sciences, Sultanpur U.P., India

  • Department of Zoology, Kamla Nehru Institute of Physical and Social Sciences, Sultanpur U.P., India

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    Rakesh Kumar Pandey, Vijai Krishna Das. (2015). Histological Changes in Calcium Regulating Endocrine Gland (Ultimobranchial) After Dimethoate Exposure in Freshwater Catfish Heteropneustes Fossilis. American Journal of Bioscience and Bioengineering, 3(5), 91-98. https://doi.org/10.11648/j.bio.20150305.22

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    Rakesh Kumar Pandey; Vijai Krishna Das. Histological Changes in Calcium Regulating Endocrine Gland (Ultimobranchial) After Dimethoate Exposure in Freshwater Catfish Heteropneustes Fossilis. Am. J. BioSci. Bioeng. 2015, 3(5), 91-98. doi: 10.11648/j.bio.20150305.22

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    AMA Style

    Rakesh Kumar Pandey, Vijai Krishna Das. Histological Changes in Calcium Regulating Endocrine Gland (Ultimobranchial) After Dimethoate Exposure in Freshwater Catfish Heteropneustes Fossilis. Am J BioSci Bioeng. 2015;3(5):91-98. doi: 10.11648/j.bio.20150305.22

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  • @article{10.11648/j.bio.20150305.22,
      author = {Rakesh Kumar Pandey and Vijai Krishna Das},
      title = {Histological Changes in Calcium Regulating Endocrine Gland (Ultimobranchial) After Dimethoate Exposure in Freshwater Catfish Heteropneustes Fossilis},
      journal = {American Journal of Bioscience and Bioengineering},
      volume = {3},
      number = {5},
      pages = {91-98},
      doi = {10.11648/j.bio.20150305.22},
      url = {https://doi.org/10.11648/j.bio.20150305.22},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.bio.20150305.22},
      abstract = {Ultimobranchial gland in fishes is associated primarily with calcium regulation. The study was aimed to find effect of an organophosphate pesticide dimethoate on serum Ca2+ and ultimobranchial gland in freshwater catfish Heteropneustes fossilis. Fish were exposed to dimethoate at 2.24 mg/l (75% of 96 h LC50) for short-term (24, 48, 72 and 96 h), and 25% of 96 h LC50 (1.00 mg/l) for long-term (6, 12, 24 and 36 d). The blood samples collected at assigned durations from the anaesthetized fish were used to measure serum Ca2+ levels. At the same time UBG were also extricated from the fish by removing the tissues adjoining esophagus, liver and sinus venosus of the heart for subsequent histological examination. A significant (P<0.05) increase in serum Ca2+ levels of fish following 24 h and 6d dimethoate exposure was observed. However, after 48 h and 12 d the Ca2+ level gradually begins to decline exhibiting significant (P<0.0001) hypocalcaemia after 96 h and 36 d in the exposed fishes. The histological examination of exposed fish glands exhibit significant changes in their cellular activity, staining property, nuclear diameter and nuclear volume, vacuolization and degeneration. It is concluded that dimethoate severely hampers the calcium homeostasis in Heteropneustes fossilis; therefore the pesticide should be cautiously used near aquatic bodies.},
     year = {2015}
    }
    

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  • TY  - JOUR
    T1  - Histological Changes in Calcium Regulating Endocrine Gland (Ultimobranchial) After Dimethoate Exposure in Freshwater Catfish Heteropneustes Fossilis
    AU  - Rakesh Kumar Pandey
    AU  - Vijai Krishna Das
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    DO  - 10.11648/j.bio.20150305.22
    T2  - American Journal of Bioscience and Bioengineering
    JF  - American Journal of Bioscience and Bioengineering
    JO  - American Journal of Bioscience and Bioengineering
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    PB  - Science Publishing Group
    SN  - 2328-5893
    UR  - https://doi.org/10.11648/j.bio.20150305.22
    AB  - Ultimobranchial gland in fishes is associated primarily with calcium regulation. The study was aimed to find effect of an organophosphate pesticide dimethoate on serum Ca2+ and ultimobranchial gland in freshwater catfish Heteropneustes fossilis. Fish were exposed to dimethoate at 2.24 mg/l (75% of 96 h LC50) for short-term (24, 48, 72 and 96 h), and 25% of 96 h LC50 (1.00 mg/l) for long-term (6, 12, 24 and 36 d). The blood samples collected at assigned durations from the anaesthetized fish were used to measure serum Ca2+ levels. At the same time UBG were also extricated from the fish by removing the tissues adjoining esophagus, liver and sinus venosus of the heart for subsequent histological examination. A significant (P<0.05) increase in serum Ca2+ levels of fish following 24 h and 6d dimethoate exposure was observed. However, after 48 h and 12 d the Ca2+ level gradually begins to decline exhibiting significant (P<0.0001) hypocalcaemia after 96 h and 36 d in the exposed fishes. The histological examination of exposed fish glands exhibit significant changes in their cellular activity, staining property, nuclear diameter and nuclear volume, vacuolization and degeneration. It is concluded that dimethoate severely hampers the calcium homeostasis in Heteropneustes fossilis; therefore the pesticide should be cautiously used near aquatic bodies.
    VL  - 3
    IS  - 5
    ER  - 

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