Histological Changes in Calcium Regulating Endocrine Gland (Ultimobranchial) After Dimethoate Exposure in Freshwater Catfish Heteropneustes Fossilis
American Journal of Bioscience and Bioengineering
Volume 3, Issue 5, October 2015, Pages: 91-98
Received: Sep. 14, 2015;
Accepted: Sep. 30, 2015;
Published: Oct. 28, 2015
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Rakesh Kumar Pandey, Department of Zoology, Kamla Nehru Institute of Physical and Social Sciences, Sultanpur U.P., India
Vijai Krishna Das, Department of Zoology, Kamla Nehru Institute of Physical and Social Sciences, Sultanpur U.P., India
Ultimobranchial gland in fishes is associated primarily with calcium regulation. The study was aimed to find effect of an organophosphate pesticide dimethoate on serum Ca2+ and ultimobranchial gland in freshwater catfish Heteropneustes fossilis. Fish were exposed to dimethoate at 2.24 mg/l (75% of 96 h LC50) for short-term (24, 48, 72 and 96 h), and 25% of 96 h LC50 (1.00 mg/l) for long-term (6, 12, 24 and 36 d). The blood samples collected at assigned durations from the anaesthetized fish were used to measure serum Ca2+ levels. At the same time UBG were also extricated from the fish by removing the tissues adjoining esophagus, liver and sinus venosus of the heart for subsequent histological examination. A significant (P<0.05) increase in serum Ca2+ levels of fish following 24 h and 6d dimethoate exposure was observed. However, after 48 h and 12 d the Ca2+ level gradually begins to decline exhibiting significant (P<0.0001) hypocalcaemia after 96 h and 36 d in the exposed fishes. The histological examination of exposed fish glands exhibit significant changes in their cellular activity, staining property, nuclear diameter and nuclear volume, vacuolization and degeneration. It is concluded that dimethoate severely hampers the calcium homeostasis in Heteropneustes fossilis; therefore the pesticide should be cautiously used near aquatic bodies.
Rakesh Kumar Pandey,
Vijai Krishna Das,
Histological Changes in Calcium Regulating Endocrine Gland (Ultimobranchial) After Dimethoate Exposure in Freshwater Catfish Heteropneustes Fossilis, American Journal of Bioscience and Bioengineering.
Vol. 3, No. 5,
2015, pp. 91-98.
Pandey RK, Malviya A and Das VK: Toxicity of cypermethrin, effects on serum electrolytes (Ca+2, Mg+2 and Pi) levels and recovery response in fresh water catfish Heteropneustes fossilis (Bloch). Int. J. Biol. Chem. Sci. 3 (5): 1182-1191, 2009a.
Pandey RK, Singh RN, Singh S, Singh NN and Das VK: Acute toxicity bioassay of dimethoate on freshwater air breathing catfish, Heteropneustes fossilis (Bloch) and its behavioural studies. J. Environ. Biol. 30 (3): 437-440, 2009 b.
Mishra D, Srivastav SK, Suzuki N and Srivastav AK: Corpuscles of Stannius of a teleost Heteropneustes fossilis following intoxication with a pyrethroid (Cypermethrin). North-Western J. Zool. 6 (2): 203-208, 2010.
Das VK, Malviya A and Pandey RK: Alterations in serum electrolytes (calcium, magnesium and phosphate) levels after dimethoate exposure and pesticide withdrawal recovery in the freshwater air-breathing catfish Heteropneustes fossilis (Bloch): Toxicol. Environ .Chem. 95(7): 1176–1182, 2013.
Pandey RK and Das VK: Dimethoate induced changes in serum Ca2+ and Corpuscles of Stannius in freshwater catfish Heteropneustes fossilis, after short-term and long-term exposure. Am. J. Life Sci. 3(1): 17-21, 2015.
Chakrabarti P and Mukherjee D: Studies on the hypocalcemic actions of salmon calcitonin and ultimobranchial gland extracts in the freshwater teleost, Cyprinus carpio. Gen. Comp. Endocrinol. 90: 267-273, 1993.
Srivastav AK, Singh S, Mishra D and Srivastav SK: Ultimobranchial gland of freshwater catfish, Heteropneustes fossilis, in response to calcitonin administration. Pesq. Vet. Bras. 29 (12): 963-968, 2009.
Srivastav AK, Srivastava SK, Mishra D, Srivastav S, Srivastav SK: Ultimobranchial gland of freshwater catfish, Heteropneustes fossilis in response to deltamethrin treatment. Bull. Environ. Contam. Toxicol. 68 (4): 584-591, 2002.
Srivastav AK, Srivastava SK, Mishra D, Srivastav SK: Histological alterations in the ultimobranchial gland of teleost Heteropneustes fossilis in response to chlorpyrifos treatment. J.Basic Clin. Physiol. Pharmacol. 22 (1-2): 23-28, 2011.
Mishra D, Srivastav SK and Srivastav AK: Effects of the insecticide cypermethrin on plasma calcium and ultimobranchial gland of a teleost, Heteropneustes fossilis. Ecotoxicol. Environ. Saf. 60 (2): 193-197, 2005.
Rai R, Mishra D,Srivastav SKand Srivastav AK: Ultimobranchial gland of a freshwater teleost, Heteropneustes fossilis, in response to cadmium treatment. Environ. Toxicol. 24 (6): 589-593, 2009.
Kumar A, Prasad MR, Suzuki N, Srivastava SK and Srivastava AK: Influence of a botanocal pesticide, azadirachtin, on ultimobranchial gland of the freshwater catfish Heteropneustes fossilis. Toxicol. Environ. Chem. 95 (10): 2013.
Suzuki N, Tabata MJ, Kambegawa A, Srivastav AK, Shimada A, Takeda H, Kobayashi M, Wada S and Catsumata T: Tributylin inhibits osteoblastic activity and disrupts calcium metabolism through an increase in plasma calcium and calcitonin levels in teleosts. Life Sci. 78 (21): 2533-25410, 2006.
APHA: Standard Methods for the Examination of Water and Wastewater, (21st Edn.), 2005. APHA, AWWA, WPCF: Washington DC, USA.
Swarup K and Norman AW: Depletion of Ca and Pi in scales, bones and muscles associated with vitamin D3 induced hypercalcemia and hyperphosphatemia in Cyprinus carpio. Proc. Natl. Acad. Sci. India. 66: 243-252, 1996.
Cengiz EI, and Unlu E: Sublethal effect of commercial Deltamethrin on the structure of the gills, liver and gut tissues of mosquito fish Gambusia affinis: a microscopic study. Environ. Toxicol. Pharm. 21: 246-253, 2006.
Yildirim MZ, Benli AC, Selvi M, Ozkul A, Erkoc F and Kocak O: Acute toxicity, behavioral changes, and histopathological effects of deltamethrin on tissues (gills, liver, brain, spleen, kidney, muscle, skin) of Nile tilapia (Oreochromis nilkoticus L.) fingerlings. Environ. Toxicol. 21: 614-620, 2006.
Velmurugan B, Selvanayagam M, Cengiz EI, Unlu E: The effects of monocrotophos to different tissues of freshwater fish Cirrhinus mrigala. Bull. Environ. Contam. Toxicol. 78: 450-454, 2007.
Peebua P, Kruatrachue M, Pokethitiyook P and Singhakaew S: Histopathological alterations of Nile tilapia, Oreochromis niloticus in acute and subchronic alachlor exposure. J. Environ. Biol. 29 (3): 325-331, 2008.
Parikh PH, Rangrez A, Bagchi RA and Desai BN: Effect of dimethoate on some histoarchitecture of freshwater fish Oreocromis mossambicus (Peters, 1852). Bioscan. 5: 55-58, 2010.
Singh NN, VK Das and Singh S: Effect of aldrin on carbohydrate, protein and ionic metabolism of a freshwater catfish, Heteropneustes fossilis. Bull. Environ. Contam. Toxicol. 57: 204-210, 1996.
Singh NN, Das VK and Srivastav AK: Formothion and propoxur induced ionic imbalance and skeletal deformity in a catfish, Heteropneustes fossilis. J. Environ. Biol. 18: 357-363, 1997.
Mishra D, Srivastav S, Srivastav SK and Srivastav AK: Plasma calcium and inorganic phosphate levels of a freshwater catfish Heteropneustes fossilis in response to Cypermethrin treatment. J. Ecophysiol. Occup. Hlth. 1: 131-138, 2001.
Mishra D, Srivastav SK and Srivastav AK: Plasma calcium and inorganic phosphate levels of a teleost, Heteropneustes fossilis exposed to metacid-50. Malaysian App. Biol. 33: 19-25, 2004.
Logaswamy S, Radha G, Subhashini S and Logankumar K: Alterations in the levels of ions in blood and liver of freshwater fish, Cyprinus carpio var. communis exposed to dimethoate. Environ. Monit. Assess. 131: 439–444, 2007.
Velisek J, Dobsikova R, Svobodova Z, Modra H and Luskova V: Effect of deltamethrin on the biochemical profile of common carp (Cyprinus carpio L.). Bull. Environ. Contam. Toxicol. 76: 992-998, 2006.
Thangavel P, Sumathiral K, Karthikeyan S and Ramaswamy M: Endocrine response of the freshwater teleost, Sarotherodon mossambicus (Peters) to dimecron exposure. Chemosphere. 61: 1083-1092, 2005.
Prasad MR, Kumar A, Mishra D, Srivastav SK and Srivastav AK: Blood electrolytes of the freshwater catfish Heteropneustes fossilis in response to treatment with a botanical pesticide (latex of Euphorbia royleana). Integrat. Zool. 6 (2): 150-156, 2011.
Thangavel P, Sumathiral K, Maheswari S, Rita S and Ramaswamy M: Hormone profile of an edible, freshwater teleost, Sarotherodon mossambicus (Peters) under endosulfan toxicity. Pestic. Biochem. Physiol. 97: 229-234, 2010.
Wendelaar Bonga, SE: Effect of synthetic salman calcitonin on plasma calcium, ultimobranchial cells, Stannius bodies and prolactin cells in the teleost Gasterosteus aculeatus. Gen. Comp. Endocrinol. 40: 99-108, 1980.
Srivastav SP, Swarup K, Singh S and Srivastav AK: Effects of calcitonin administration on ultimobranchial gland, Stannius corpuscles and prolactin cells in male catfish, Clarias batrachus. Arch. Biol. 100: 385-392, 1989.